Salmonella phage Vi06
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1XU86|E1XU86_9CAUD DNA ligase OS=Salmonella phage Vi06 OX=866889 GN=1.3 PE=4 SV=1
MM1 pKa = 7.01 GHH3 pKa = 7.58 LYY5 pKa = 10.47 SGSISDD11 pKa = 4.35 FKK13 pKa = 11.37 AATGKK18 pKa = 10.41 LLEE21 pKa = 4.55 LDD23 pKa = 4.39 FVVKK27 pKa = 10.7 CDD29 pKa = 3.37 DD30 pKa = 3.77 WYY32 pKa = 10.67 SSHH35 pKa = 6.38 MGSNFMCLHH44 pKa = 6.59 IEE46 pKa = 4.31 DD47 pKa = 4.39 MDD49 pKa = 4.49 GNSLITNIFCHH60 pKa = 6.36 YY61 pKa = 10.65 DD62 pKa = 3.36 EE63 pKa = 6.48 DD64 pKa = 4.21 ILYY67 pKa = 10.94 SMCTEE72 pKa = 4.3 WLNRR76 pKa = 11.84 MYY78 pKa = 10.95 DD79 pKa = 3.4 QLKK82 pKa = 10.17 DD83 pKa = 3.29 WKK85 pKa = 10.79
Molecular weight: 9.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.327
IPC2_protein 4.52
IPC_protein 4.418
Toseland 4.24
ProMoST 4.584
Dawson 4.418
Bjellqvist 4.571
Wikipedia 4.368
Rodwell 4.266
Grimsley 4.151
Solomon 4.418
Lehninger 4.368
Nozaki 4.546
DTASelect 4.787
Thurlkill 4.291
EMBOSS 4.38
Sillero 4.558
Patrickios 0.744
IPC_peptide 4.406
IPC2_peptide 4.546
IPC2.peptide.svr19 4.427
Protein with the highest isoelectric point:
>tr|E1XU89|E1XU89_9CAUD Conserved hypothetical phage protein OS=Salmonella phage Vi06 OX=866889 GN=1.7 PE=4 SV=1
MM1 pKa = 7.4 FRR3 pKa = 11.84 LHH5 pKa = 6.51 YY6 pKa = 10.11 NQSLEE11 pKa = 4.03 NFTVRR16 pKa = 11.84 RR17 pKa = 11.84 PDD19 pKa = 3.24 RR20 pKa = 11.84 SIVCASEE27 pKa = 3.52 YY28 pKa = 10.63 HH29 pKa = 6.33 RR30 pKa = 11.84 AKK32 pKa = 10.43 LPLMGDD38 pKa = 4.24 TVPLTPGVHH47 pKa = 7.08 LIITRR52 pKa = 11.84 GDD54 pKa = 3.32 FKK56 pKa = 11.18 KK57 pKa = 10.81 AIDD60 pKa = 3.64 KK61 pKa = 10.23 KK62 pKa = 10.93 RR63 pKa = 11.84 PVLSVAVTRR72 pKa = 11.84 FPFACLLVKK81 pKa = 10.48 RR82 pKa = 11.84 IKK84 pKa = 10.89 EE85 pKa = 3.93 MLL87 pKa = 3.84
Molecular weight: 10.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.283
IPC2_protein 9.516
IPC_protein 9.794
Toseland 10.555
ProMoST 10.116
Dawson 10.643
Bjellqvist 10.292
Wikipedia 10.789
Rodwell 11.052
Grimsley 10.687
Solomon 10.716
Lehninger 10.687
Nozaki 10.54
DTASelect 10.277
Thurlkill 10.54
EMBOSS 10.935
Sillero 10.57
Patrickios 10.818
IPC_peptide 10.716
IPC2_peptide 9.151
IPC2.peptide.svr19 8.556
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
10943
34
1318
232.8
25.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.855 ± 0.505
1.225 ± 0.219
6.214 ± 0.269
6.707 ± 0.377
3.39 ± 0.209
7.731 ± 0.371
1.864 ± 0.186
5.209 ± 0.164
6.452 ± 0.321
7.831 ± 0.293
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.235 ± 0.212
4.396 ± 0.297
3.409 ± 0.167
3.628 ± 0.296
5.876 ± 0.245
6.516 ± 0.194
6.022 ± 0.294
6.543 ± 0.285
1.48 ± 0.162
3.418 ± 0.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here