Salmonella phage Vi06

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus; Salmonella virus Vi06

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|E1XU86|E1XU86_9CAUD DNA ligase OS=Salmonella phage Vi06 OX=866889 GN=1.3 PE=4 SV=1
MM1 pKa = 7.01GHH3 pKa = 7.58LYY5 pKa = 10.47SGSISDD11 pKa = 4.35FKK13 pKa = 11.37AATGKK18 pKa = 10.41LLEE21 pKa = 4.55LDD23 pKa = 4.39FVVKK27 pKa = 10.7CDD29 pKa = 3.37DD30 pKa = 3.77WYY32 pKa = 10.67SSHH35 pKa = 6.38MGSNFMCLHH44 pKa = 6.59IEE46 pKa = 4.31DD47 pKa = 4.39MDD49 pKa = 4.49GNSLITNIFCHH60 pKa = 6.36YY61 pKa = 10.65DD62 pKa = 3.36EE63 pKa = 6.48DD64 pKa = 4.21ILYY67 pKa = 10.94SMCTEE72 pKa = 4.3WLNRR76 pKa = 11.84MYY78 pKa = 10.95DD79 pKa = 3.4QLKK82 pKa = 10.17DD83 pKa = 3.29WKK85 pKa = 10.79

Molecular weight:
9.99 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|E1XU89|E1XU89_9CAUD Conserved hypothetical phage protein OS=Salmonella phage Vi06 OX=866889 GN=1.7 PE=4 SV=1
MM1 pKa = 7.4FRR3 pKa = 11.84LHH5 pKa = 6.51YY6 pKa = 10.11NQSLEE11 pKa = 4.03NFTVRR16 pKa = 11.84RR17 pKa = 11.84PDD19 pKa = 3.24RR20 pKa = 11.84SIVCASEE27 pKa = 3.52YY28 pKa = 10.63HH29 pKa = 6.33RR30 pKa = 11.84AKK32 pKa = 10.43LPLMGDD38 pKa = 4.24TVPLTPGVHH47 pKa = 7.08LIITRR52 pKa = 11.84GDD54 pKa = 3.32FKK56 pKa = 11.18KK57 pKa = 10.81AIDD60 pKa = 3.64KK61 pKa = 10.23KK62 pKa = 10.93RR63 pKa = 11.84PVLSVAVTRR72 pKa = 11.84FPFACLLVKK81 pKa = 10.48RR82 pKa = 11.84IKK84 pKa = 10.89EE85 pKa = 3.93MLL87 pKa = 3.84

Molecular weight:
10.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

10943

34

1318

232.8

25.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.855 ± 0.505

1.225 ± 0.219

6.214 ± 0.269

6.707 ± 0.377

3.39 ± 0.209

7.731 ± 0.371

1.864 ± 0.186

5.209 ± 0.164

6.452 ± 0.321

7.831 ± 0.293

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.235 ± 0.212

4.396 ± 0.297

3.409 ± 0.167

3.628 ± 0.296

5.876 ± 0.245

6.516 ± 0.194

6.022 ± 0.294

6.543 ± 0.285

1.48 ± 0.162

3.418 ± 0.216

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski