Nannochloropsis gaditana

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; Eustigmatophyceae; Eustigmatales; Monodopsidaceae; Nannochloropsis

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10819 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7T8I0|W7T8I0_9STRA Light-harvesting protein OS=Nannochloropsis gaditana OX=72520 GN=Naga_100641g3 PE=3 SV=1
MM1 pKa = 7.54EE2 pKa = 4.54VRR4 pKa = 11.84NVLDD8 pKa = 4.55DD9 pKa = 3.4SHH11 pKa = 6.81EE12 pKa = 4.39AFDD15 pKa = 5.09CYY17 pKa = 11.34SFICIRR23 pKa = 11.84ICVFHH28 pKa = 6.74EE29 pKa = 3.94AVASNFTGAGMSVTNSVKK47 pKa = 10.57VGTGFEE53 pKa = 4.26LQSGPFMATTDD64 pKa = 3.68NFDD67 pKa = 3.5VQSDD71 pKa = 4.94DD72 pKa = 2.9ITMSYY77 pKa = 11.15SNSFQLSVTSGTAMRR92 pKa = 11.84AGDD95 pKa = 3.51ISLSEE100 pKa = 4.13AAAQIYY106 pKa = 7.56TEE108 pKa = 4.36PGVLRR113 pKa = 11.84LSTGAATFAMAARR126 pKa = 11.84GPLSVLQTKK135 pKa = 10.62ADD137 pKa = 3.64VYY139 pKa = 10.86EE140 pKa = 4.11QVADD144 pKa = 3.72EE145 pKa = 4.29WSLNSGGDD153 pKa = 3.01IDD155 pKa = 4.12VTSNGTLSMQSVSGDD170 pKa = 3.44LTFDD174 pKa = 3.54EE175 pKa = 5.25LQVEE179 pKa = 4.44TDD181 pKa = 3.18TFAIEE186 pKa = 4.29SVDD189 pKa = 3.3ILVDD193 pKa = 3.63SSEE196 pKa = 4.17TLEE199 pKa = 4.1MVVGNATTLTSPFVSHH215 pKa = 7.15FSDD218 pKa = 3.44VTLVEE223 pKa = 4.37SSEE226 pKa = 4.48SITWRR231 pKa = 11.84TGNASLSADD240 pKa = 3.21AVTIDD245 pKa = 3.49SSEE248 pKa = 4.09TLEE251 pKa = 4.1MVVGNATTLTSPFVSHH267 pKa = 7.16FSDD270 pKa = 3.37VTLPLCRR277 pKa = 11.84CGCDD281 pKa = 2.88

Molecular weight: 29.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7TI78|W7TI78_9STRA Ankyrin domain protein OS=Nannochloropsis gaditana OX=72520 GN=Naga_100001g29 PE=4 SV=1
MM1 pKa = 7.13TLKK4 pKa = 9.71LTRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84WPLMTMSRR17 pKa = 11.84TMTLKK22 pKa = 9.52LTRR25 pKa = 11.84RR26 pKa = 11.84LWPLMTMSRR35 pKa = 11.84TMTLKK40 pKa = 9.23LTRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84WPPMTMSRR53 pKa = 11.84TMTSKK58 pKa = 9.24LTRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84WPLMTMSRR71 pKa = 11.84TMTSKK76 pKa = 9.24LTRR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84WPLMTMSRR89 pKa = 11.84TMTLKK94 pKa = 9.23LTRR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84WPPMTMSRR107 pKa = 11.84MRR109 pKa = 11.84SRR111 pKa = 11.84MMTRR115 pKa = 11.84RR116 pKa = 11.84AGMKK120 pKa = 8.58CTTKK124 pKa = 10.25RR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84LWPPMTMSRR136 pKa = 11.84MRR138 pKa = 11.84SRR140 pKa = 11.84MMTKK144 pKa = 10.07RR145 pKa = 11.84AGMKK149 pKa = 8.74CTTKK153 pKa = 10.25RR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84LWPPMTMSRR165 pKa = 11.84TRR167 pKa = 11.84SRR169 pKa = 11.84MMTRR173 pKa = 11.84RR174 pKa = 11.84AGMKK178 pKa = 8.58CTTKK182 pKa = 10.25RR183 pKa = 11.84RR184 pKa = 11.84RR185 pKa = 11.84LWPPMTMSRR194 pKa = 11.84TRR196 pKa = 11.84SRR198 pKa = 11.84MMTRR202 pKa = 11.84RR203 pKa = 11.84AGMKK207 pKa = 8.58CTTKK211 pKa = 10.25RR212 pKa = 11.84RR213 pKa = 11.84RR214 pKa = 11.84RR215 pKa = 3.54

Molecular weight: 26.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10493

326

10819

4433024

29

5587

409.7

44.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.286 ± 0.025

1.617 ± 0.01

4.677 ± 0.016

6.865 ± 0.03

3.497 ± 0.014

8.533 ± 0.029

2.471 ± 0.011

3.542 ± 0.016

4.47 ± 0.019

9.844 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.009

2.641 ± 0.014

6.007 ± 0.023

3.706 ± 0.02

7.248 ± 0.022

7.967 ± 0.029

5.144 ± 0.014

6.632 ± 0.018

1.308 ± 0.009

2.256 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski