Amino acid dipepetide frequency for Nannochloropsis gaditana

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.365AlaAla: 10.365 ± 0.076	1.721AlaCys: 1.721 ± 0.023	3.667AlaAsp: 3.667 ± 0.029	5.396AlaGlu: 5.396 ± 0.046	3.725AlaPhe: 3.725 ± 0.029	7.475AlaGly: 7.475 ± 0.046	2.092AlaHis: 2.092 ± 0.023	3.164AlaIle: 3.164 ± 0.031	3.691AlaLys: 3.691 ± 0.037	10.034AlaLeu: 10.034 ± 0.067	2.225AlaMet: 2.225 ± 0.026	1.987AlaAsn: 1.987 ± 0.022	5.074AlaPro: 5.074 ± 0.041	3.186AlaGln: 3.186 ± 0.031	6.473AlaArg: 6.473 ± 0.046	7.998AlaSer: 7.998 ± 0.049	4.905AlaThr: 4.905 ± 0.037	6.295AlaVal: 6.295 ± 0.04	1.333AlaTrp: 1.333 ± 0.02	2.048AlaTyr: 2.048 ± 0.025	0.0AlaXaa: 0.0 ± 0.0
Cys	1.27CysAla: 1.27 ± 0.021	0.384CysCys: 0.384 ± 0.012	0.693CysAsp: 0.693 ± 0.014	0.781CysGlu: 0.781 ± 0.013	0.684CysPhe: 0.684 ± 0.012	1.264CysGly: 1.264 ± 0.019	0.434CysHis: 0.434 ± 0.012	0.649CysIle: 0.649 ± 0.013	0.599CysLys: 0.599 ± 0.014	1.94CysLeu: 1.94 ± 0.023	0.387CysMet: 0.387 ± 0.01	0.451CysAsn: 0.451 ± 0.011	1.042CysPro: 1.042 ± 0.017	0.602CysGln: 0.602 ± 0.013	1.204CysArg: 1.204 ± 0.019	1.217CysSer: 1.217 ± 0.017	0.861CysThr: 0.861 ± 0.015	1.119CysVal: 1.119 ± 0.016	0.228CysTrp: 0.228 ± 0.007	0.365CysTyr: 0.365 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	4.372AspAla: 4.372 ± 0.034	0.661AspCys: 0.661 ± 0.013	2.689AspAsp: 2.689 ± 0.035	3.635AspGlu: 3.635 ± 0.029	1.783AspPhe: 1.783 ± 0.019	4.255AspGly: 4.255 ± 0.033	0.952AspHis: 0.952 ± 0.016	1.822AspIle: 1.822 ± 0.023	2.046AspLys: 2.046 ± 0.024	4.517AspLeu: 4.517 ± 0.035	1.268AspMet: 1.268 ± 0.018	1.133AspAsn: 1.133 ± 0.016	2.665AspPro: 2.665 ± 0.024	1.36AspGln: 1.36 ± 0.019	2.968AspArg: 2.968 ± 0.027	2.891AspSer: 2.891 ± 0.026	2.199AspThr: 2.199 ± 0.022	3.768AspVal: 3.768 ± 0.035	0.668AspTrp: 0.668 ± 0.014	1.122AspTyr: 1.122 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	6.95GluAla: 6.95 ± 0.054	0.776GluCys: 0.776 ± 0.015	4.293GluAsp: 4.293 ± 0.037	9.147GluGlu: 9.147 ± 0.099	1.369GluPhe: 1.369 ± 0.018	8.129GluGly: 8.129 ± 0.069	1.335GluHis: 1.335 ± 0.018	2.326GluIle: 2.326 ± 0.023	3.799GluLys: 3.799 ± 0.036	4.73GluLeu: 4.73 ± 0.039	1.667GluMet: 1.667 ± 0.021	1.877GluAsn: 1.877 ± 0.021	2.022GluPro: 2.022 ± 0.026	2.213GluGln: 2.213 ± 0.024	4.895GluArg: 4.895 ± 0.047	3.706GluSer: 3.706 ± 0.031	3.054GluThr: 3.054 ± 0.023	4.305GluVal: 4.305 ± 0.035	0.814GluTrp: 0.814 ± 0.014	1.246GluTyr: 1.246 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	2.746PheAla: 2.746 ± 0.028	0.66PheCys: 0.66 ± 0.014	1.692PheAsp: 1.692 ± 0.021	1.781PheGlu: 1.781 ± 0.022	1.724PhePhe: 1.724 ± 0.023	2.369PheGly: 2.369 ± 0.028	0.946PheHis: 0.946 ± 0.015	1.331PheIle: 1.331 ± 0.018	1.306PheLys: 1.306 ± 0.022	4.428PheLeu: 4.428 ± 0.039	0.753PheMet: 0.753 ± 0.012	0.964PheAsn: 0.964 ± 0.014	2.282PhePro: 2.282 ± 0.021	1.345PheGln: 1.345 ± 0.018	2.078PheArg: 2.078 ± 0.02	2.956PheSer: 2.956 ± 0.028	1.652PheThr: 1.652 ± 0.02	2.45PheVal: 2.45 ± 0.025	0.515PheTrp: 0.515 ± 0.013	0.986PheTyr: 0.986 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	7.07GlyAla: 7.07 ± 0.045	1.19GlyCys: 1.19 ± 0.017	3.708GlyAsp: 3.708 ± 0.032	6.176GlyGlu: 6.176 ± 0.054	2.483GlyPhe: 2.483 ± 0.027	11.422GlyGly: 11.422 ± 0.127	1.87GlyHis: 1.87 ± 0.02	2.879GlyIle: 2.879 ± 0.029	4.721GlyLys: 4.721 ± 0.037	6.956GlyLeu: 6.956 ± 0.043	2.121GlyMet: 2.121 ± 0.026	2.423GlyAsn: 2.423 ± 0.033	3.816GlyPro: 3.816 ± 0.039	2.864GlyGln: 2.864 ± 0.029	7.483GlyArg: 7.483 ± 0.061	5.967GlySer: 5.967 ± 0.046	4.013GlyThr: 4.013 ± 0.032	5.321GlyVal: 5.321 ± 0.043	1.194GlyTrp: 1.194 ± 0.018	1.654GlyTyr: 1.654 ± 0.023	0.0GlyXaa: 0.0 ± 0.0
His	2.39HisAla: 2.39 ± 0.025	0.437HisCys: 0.437 ± 0.01	1.071HisAsp: 1.071 ± 0.016	1.259HisGlu: 1.259 ± 0.016	0.963HisPhe: 0.963 ± 0.018	1.928HisGly: 1.928 ± 0.022	0.952HisHis: 0.952 ± 0.021	0.888HisIle: 0.888 ± 0.015	0.88HisLys: 0.88 ± 0.015	2.516HisLeu: 2.516 ± 0.027	0.524HisMet: 0.524 ± 0.012	0.594HisAsn: 0.594 ± 0.011	1.781HisPro: 1.781 ± 0.025	1.015HisGln: 1.015 ± 0.02	1.843HisArg: 1.843 ± 0.024	1.707HisSer: 1.707 ± 0.019	1.177HisThr: 1.177 ± 0.018	1.856HisVal: 1.856 ± 0.023	0.303HisTrp: 0.303 ± 0.008	0.624HisTyr: 0.624 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	2.972IleAla: 2.972 ± 0.033	0.667IleCys: 0.667 ± 0.012	1.829IleAsp: 1.829 ± 0.023	2.046IleGlu: 2.046 ± 0.022	1.544IlePhe: 1.544 ± 0.023	2.353IleGly: 2.353 ± 0.028	0.91IleHis: 0.91 ± 0.016	1.462IleIle: 1.462 ± 0.022	1.725IleLys: 1.725 ± 0.022	3.867IleLeu: 3.867 ± 0.033	0.859IleMet: 0.859 ± 0.015	1.134IleAsn: 1.134 ± 0.018	2.098IlePro: 2.098 ± 0.023	1.355IleGln: 1.355 ± 0.017	2.277IleArg: 2.277 ± 0.022	2.709IleSer: 2.709 ± 0.03	1.794IleThr: 1.794 ± 0.023	2.406IleVal: 2.406 ± 0.024	0.448IleTrp: 0.448 ± 0.01	0.958IleTyr: 0.958 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	4.454LysAla: 4.454 ± 0.041	0.568LysCys: 0.568 ± 0.013	2.376LysAsp: 2.376 ± 0.024	4.184LysGlu: 4.184 ± 0.043	1.059LysPhe: 1.059 ± 0.016	3.987LysGly: 3.987 ± 0.036	1.026LysHis: 1.026 ± 0.016	1.7LysIle: 1.7 ± 0.024	3.096LysLys: 3.096 ± 0.044	3.356LysLeu: 3.356 ± 0.028	1.134LysMet: 1.134 ± 0.015	1.373LysAsn: 1.373 ± 0.02	1.888LysPro: 1.888 ± 0.023	1.575LysGln: 1.575 ± 0.019	3.521LysArg: 3.521 ± 0.031	2.737LysSer: 2.737 ± 0.027	2.312LysThr: 2.312 ± 0.023	2.823LysVal: 2.823 ± 0.029	0.506LysTrp: 0.506 ± 0.012	1.027LysTyr: 1.027 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	9.147LeuAla: 9.147 ± 0.053	1.721LeuCys: 1.721 ± 0.018	4.549LeuAsp: 4.549 ± 0.034	5.971LeuGlu: 5.971 ± 0.045	3.549LeuPhe: 3.549 ± 0.033	6.798LeuGly: 6.798 ± 0.041	2.609LeuHis: 2.609 ± 0.024	3.14LeuIle: 3.14 ± 0.035	4.023LeuLys: 4.023 ± 0.033	11.194LeuLeu: 11.194 ± 0.084	1.951LeuMet: 1.951 ± 0.022	2.499LeuAsn: 2.499 ± 0.025	7.235LeuPro: 7.235 ± 0.055	4.101LeuGln: 4.101 ± 0.035	7.089LeuArg: 7.089 ± 0.047	7.994LeuSer: 7.994 ± 0.053	4.872LeuThr: 4.872 ± 0.035	6.498LeuVal: 6.498 ± 0.04	1.221LeuTrp: 1.221 ± 0.018	2.278LeuTyr: 2.278 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	2.451MetAla: 2.451 ± 0.025	0.321MetCys: 0.321 ± 0.008	1.291MetAsp: 1.291 ± 0.018	1.779MetGlu: 1.779 ± 0.018	0.573MetPhe: 0.573 ± 0.012	1.861MetGly: 1.861 ± 0.023	0.559MetHis: 0.559 ± 0.012	0.762MetIle: 0.762 ± 0.014	1.042MetLys: 1.042 ± 0.015	2.082MetLeu: 2.082 ± 0.021	0.599MetMet: 0.599 ± 0.012	0.622MetAsn: 0.622 ± 0.013	1.257MetPro: 1.257 ± 0.018	0.884MetGln: 0.884 ± 0.016	1.578MetArg: 1.578 ± 0.021	1.623MetSer: 1.623 ± 0.021	1.314MetThr: 1.314 ± 0.019	1.527MetVal: 1.527 ± 0.018	0.257MetTrp: 0.257 ± 0.007	0.498MetTyr: 0.498 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.451AsnAla: 2.451 ± 0.022	0.379AsnCys: 0.379 ± 0.01	1.243AsnAsp: 1.243 ± 0.016	1.578AsnGlu: 1.578 ± 0.021	1.068AsnPhe: 1.068 ± 0.018	2.258AsnGly: 2.258 ± 0.03	0.628AsnHis: 0.628 ± 0.012	1.191AsnIle: 1.191 ± 0.017	1.26AsnLys: 1.26 ± 0.018	2.558AsnLeu: 2.558 ± 0.027	0.666AsnMet: 0.666 ± 0.014	0.881AsnAsn: 0.881 ± 0.041	1.571AsnPro: 1.571 ± 0.02	0.907AsnGln: 0.907 ± 0.028	1.649AsnArg: 1.649 ± 0.02	1.772AsnSer: 1.772 ± 0.024	1.379AsnThr: 1.379 ± 0.019	1.982AsnVal: 1.982 ± 0.022	0.322AsnTrp: 0.322 ± 0.011	0.668AsnTyr: 0.668 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	5.392ProAla: 5.392 ± 0.043	0.956ProCys: 0.956 ± 0.016	2.293ProAsp: 2.293 ± 0.023	2.999ProGlu: 2.999 ± 0.029	2.319ProPhe: 2.319 ± 0.025	4.183ProGly: 4.183 ± 0.038	1.497ProHis: 1.497 ± 0.022	1.69ProIle: 1.69 ± 0.023	1.777ProLys: 1.777 ± 0.021	5.752ProLeu: 5.752 ± 0.037	1.0ProMet: 1.0 ± 0.015	1.275ProAsn: 1.275 ± 0.021	6.842ProPro: 6.842 ± 0.099	1.945ProGln: 1.945 ± 0.023	3.762ProArg: 3.762 ± 0.035	7.392ProSer: 7.392 ± 0.072	3.388ProThr: 3.388 ± 0.044	3.633ProVal: 3.633 ± 0.031	0.721ProTrp: 0.721 ± 0.013	1.252ProTyr: 1.252 ± 0.017	0.0ProXaa: 0.0 ± 0.0
Gln	4.051GlnAla: 4.051 ± 0.033	0.527GlnCys: 0.527 ± 0.012	1.861GlnAsp: 1.861 ± 0.024	2.911GlnGlu: 2.911 ± 0.028	0.845GlnPhe: 0.845 ± 0.013	3.155GlnGly: 3.155 ± 0.032	1.159GlnHis: 1.159 ± 0.02	1.256GlnIle: 1.256 ± 0.019	1.632GlnLys: 1.632 ± 0.023	2.951GlnLeu: 2.951 ± 0.029	0.696GlnMet: 0.696 ± 0.013	0.989GlnAsn: 0.989 ± 0.017	1.884GlnPro: 1.884 ± 0.026	2.64GlnGln: 2.64 ± 0.107	2.764GlnArg: 2.764 ± 0.025	2.376GlnSer: 2.376 ± 0.026	1.72GlnThr: 1.72 ± 0.02	2.353GlnVal: 2.353 ± 0.023	0.452GlnTrp: 0.452 ± 0.018	0.836GlnTyr: 0.836 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	6.289ArgAla: 6.289 ± 0.044	1.109ArgCys: 1.109 ± 0.018	3.201ArgAsp: 3.201 ± 0.032	5.699ArgGlu: 5.699 ± 0.047	2.24ArgPhe: 2.24 ± 0.02	6.145ArgGly: 6.145 ± 0.045	1.924ArgHis: 1.924 ± 0.022	2.47ArgIle: 2.47 ± 0.024	3.666ArgLys: 3.666 ± 0.033	6.946ArgLeu: 6.946 ± 0.043	1.677ArgMet: 1.677 ± 0.019	1.894ArgAsn: 1.894 ± 0.021	3.948ArgPro: 3.948 ± 0.034	2.958ArgGln: 2.958 ± 0.03	7.147ArgArg: 7.147 ± 0.064	4.731ArgSer: 4.731 ± 0.041	3.314ArgThr: 3.314 ± 0.03	4.588ArgVal: 4.588 ± 0.033	0.996ArgTrp: 0.996 ± 0.017	1.541ArgTyr: 1.541 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	6.372SerAla: 6.372 ± 0.041	1.343SerCys: 1.343 ± 0.021	2.954SerAsp: 2.954 ± 0.028	3.618SerGlu: 3.618 ± 0.028	3.305SerPhe: 3.305 ± 0.027	5.635SerGly: 5.635 ± 0.039	1.999SerHis: 1.999 ± 0.024	2.981SerIle: 2.981 ± 0.028	3.064SerLys: 3.064 ± 0.027	8.592SerLeu: 8.592 ± 0.056	1.771SerMet: 1.771 ± 0.021	2.096SerAsn: 2.096 ± 0.025	5.83SerPro: 5.83 ± 0.058	2.566SerGln: 2.566 ± 0.025	5.208SerArg: 5.208 ± 0.039	10.136SerSer: 10.136 ± 0.098	4.973SerThr: 4.973 ± 0.036	4.591SerVal: 4.591 ± 0.033	0.961SerTrp: 0.961 ± 0.017	1.678SerTyr: 1.678 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	4.988ThrAla: 4.988 ± 0.039	0.976ThrCys: 0.976 ± 0.016	2.134ThrAsp: 2.134 ± 0.022	2.769ThrGlu: 2.769 ± 0.026	1.968ThrPhe: 1.968 ± 0.024	4.287ThrGly: 4.287 ± 0.032	1.195ThrHis: 1.195 ± 0.016	1.898ThrIle: 1.898 ± 0.028	1.957ThrLys: 1.957 ± 0.023	5.241ThrLeu: 5.241 ± 0.033	1.127ThrMet: 1.127 ± 0.018	1.344ThrAsn: 1.344 ± 0.02	3.403ThrPro: 3.403 ± 0.037	1.706ThrGln: 1.706 ± 0.019	3.357ThrArg: 3.357 ± 0.028	4.732ThrSer: 4.732 ± 0.044	3.145ThrThr: 3.145 ± 0.04	3.307ThrVal: 3.307 ± 0.027	0.668ThrTrp: 0.668 ± 0.013	1.24ThrTyr: 1.24 ± 0.016	0.0ThrXaa: 0.0 ± 0.0
Val	6.189ValAla: 6.189 ± 0.036	1.121ValCys: 1.121 ± 0.016	3.411ValAsp: 3.411 ± 0.03	4.676ValGlu: 4.676 ± 0.038	2.38ValPhe: 2.38 ± 0.023	5.152ValGly: 5.152 ± 0.041	1.607ValHis: 1.607 ± 0.02	2.404ValIle: 2.404 ± 0.024	2.86ValLys: 2.86 ± 0.029	7.114ValLeu: 7.114 ± 0.047	1.559ValMet: 1.559 ± 0.019	1.739ValAsn: 1.739 ± 0.017	3.666ValPro: 3.666 ± 0.033	2.388ValGln: 2.388 ± 0.023	4.44ValArg: 4.44 ± 0.035	4.644ValSer: 4.644 ± 0.028	3.395ValThr: 3.395 ± 0.033	5.117ValVal: 5.117 ± 0.046	0.896ValTrp: 0.896 ± 0.015	1.564ValTyr: 1.564 ± 0.021	0.0ValXaa: 0.0 ± 0.0
Trp	1.084TrpAla: 1.084 ± 0.015	0.229TrpCys: 0.229 ± 0.007	0.646TrpAsp: 0.646 ± 0.012	0.976TrpGlu: 0.976 ± 0.016	0.371TrpPhe: 0.371 ± 0.01	1.013TrpGly: 1.013 ± 0.018	0.326TrpHis: 0.326 ± 0.009	0.477TrpIle: 0.477 ± 0.011	0.676TrpLys: 0.676 ± 0.013	1.194TrpLeu: 1.194 ± 0.018	0.347TrpMet: 0.347 ± 0.009	0.438TrpAsn: 0.438 ± 0.026	0.579TrpPro: 0.579 ± 0.013	0.531TrpGln: 0.531 ± 0.01	1.169TrpArg: 1.169 ± 0.019	0.902TrpSer: 0.902 ± 0.015	0.759TrpThr: 0.759 ± 0.013	0.822TrpVal: 0.822 ± 0.014	0.248TrpTrp: 0.248 ± 0.009	0.294TrpTyr: 0.294 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.854TyrAla: 1.854 ± 0.022	0.426TyrCys: 0.426 ± 0.011	1.174TyrAsp: 1.174 ± 0.017	1.27TyrGlu: 1.27 ± 0.018	1.012TyrPhe: 1.012 ± 0.015	1.662TyrGly: 1.662 ± 0.022	0.688TyrHis: 0.688 ± 0.012	0.924TyrIle: 0.924 ± 0.015	0.88TyrLys: 0.88 ± 0.014	2.47TyrLeu: 2.47 ± 0.028	0.549TyrMet: 0.549 ± 0.013	0.7TyrAsn: 0.7 ± 0.014	1.187TyrPro: 1.187 ± 0.02	0.916TyrGln: 0.916 ± 0.014	1.578TyrArg: 1.578 ± 0.019	1.484TyrSer: 1.484 ± 0.018	1.212TyrThr: 1.212 ± 0.018	1.559TyrVal: 1.559 ± 0.019	0.333TyrTrp: 0.333 ± 0.008	0.686TyrTyr: 0.686 ± 0.013	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10819 proteins (4433024 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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