Cruoricaptor ignavus
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2166 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6BJH3|A0A1M6BJH3_9FLAO Uncharacterized protein OS=Cruoricaptor ignavus OX=1118202 GN=SAMN05443429_10229 PE=4 SV=1
MM1 pKa = 7.23 YY2 pKa = 10.14 YY3 pKa = 10.19 YY4 pKa = 10.61 YY5 pKa = 10.85 QFFFFSCNEE14 pKa = 4.14 DD15 pKa = 3.2 EE16 pKa = 6.31 SLDD19 pKa = 3.51 QSPINEE25 pKa = 4.01 NLITSFNKK33 pKa = 10.31 SSTTSKK39 pKa = 10.84 NSDD42 pKa = 2.77 ISEE45 pKa = 4.35 VIVFSDD51 pKa = 3.37 MTSSDD56 pKa = 3.71 EE57 pKa = 4.18 NLSGMLEE64 pKa = 4.02 GLIVSVDD71 pKa = 3.92 LDD73 pKa = 3.62 AMKK76 pKa = 9.75 ATFTNEE82 pKa = 3.42 NEE84 pKa = 4.05 AGSIEE89 pKa = 4.07 FDD91 pKa = 4.27 LIEE94 pKa = 4.79 NEE96 pKa = 3.82 NSTLSLSGYY105 pKa = 5.52 TAKK108 pKa = 10.85 NGLSDD113 pKa = 3.67 RR114 pKa = 11.84 TPRR117 pKa = 11.84 WLCGASCVGQGFLISLWDD135 pKa = 4.11 GPAPLMDD142 pKa = 3.48 IAAAAFKK149 pKa = 10.72 LYY151 pKa = 10.97
Molecular weight: 16.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.592
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.617
Grimsley 3.503
Solomon 3.757
Lehninger 3.706
Nozaki 3.897
DTASelect 4.075
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.91
Patrickios 0.896
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|A0A1M6GYM3|A0A1M6GYM3_9FLAO Protein involved in gliding motility GldC OS=Cruoricaptor ignavus OX=1118202 GN=SAMN05443429_11077 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.59 RR7 pKa = 11.84 KK8 pKa = 7.05 RR9 pKa = 11.84 HH10 pKa = 5.11 KK11 pKa = 10.59 VATHH15 pKa = 5.21 KK16 pKa = 10.3 RR17 pKa = 11.84 KK18 pKa = 9.62 KK19 pKa = 9.04 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 ANRR25 pKa = 11.84 HH26 pKa = 4.77 KK27 pKa = 10.58 KK28 pKa = 9.8 KK29 pKa = 10.51 KK30 pKa = 10.04
Molecular weight: 3.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2166
0
2166
683640
26
2358
315.6
35.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.325 ± 0.058
0.721 ± 0.015
5.304 ± 0.037
7.223 ± 0.061
5.388 ± 0.046
6.68 ± 0.051
1.676 ± 0.023
7.307 ± 0.05
7.468 ± 0.053
9.076 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.384 ± 0.022
5.669 ± 0.05
3.634 ± 0.028
3.668 ± 0.031
4.421 ± 0.036
6.22 ± 0.038
5.008 ± 0.036
5.967 ± 0.036
1.039 ± 0.018
3.821 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here