Trichomalopsis sarcophagae
Average proteome isoelectric point is 7.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16064 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A232FGZ8|A0A232FGZ8_9HYME Coiled-coil domain-containing protein 86 OS=Trichomalopsis sarcophagae OX=543379 GN=TSAR_010731 PE=4 SV=1
MM1 pKa = 7.39 LRR3 pKa = 11.84 DD4 pKa = 3.84 ANDD7 pKa = 5.09 DD8 pKa = 3.22 IHH10 pKa = 8.6 ICICDD15 pKa = 3.86 VIFLLHH21 pKa = 5.34 VQEE24 pKa = 5.99 DD25 pKa = 3.64 ITIFQIDD32 pKa = 3.77 STYY35 pKa = 8.76 EE36 pKa = 3.79 TVPEE40 pKa = 4.14 VEE42 pKa = 5.44 GAMQLCTLIVIYY54 pKa = 10.23 HH55 pKa = 5.15 GHH57 pKa = 6.09 EE58 pKa = 5.5 DD59 pKa = 3.05 ITIFQIDD66 pKa = 3.77 STYY69 pKa = 8.76 EE70 pKa = 3.79 TVPEE74 pKa = 4.14 VEE76 pKa = 5.44 GAMQLCTLIVIYY88 pKa = 10.16 HH89 pKa = 6.15 GHH91 pKa = 7.7 PDD93 pKa = 3.61 PDD95 pKa = 4.28 LDD97 pKa = 4.11 PEE99 pKa = 4.44 LVIEE103 pKa = 4.7 PEE105 pKa = 4.11 FEE107 pKa = 4.46 LEE109 pKa = 4.27 PEE111 pKa = 4.19 RR112 pKa = 11.84 QLEE115 pKa = 4.3 PEE117 pKa = 4.63 LEE119 pKa = 4.2 LEE121 pKa = 4.45 PEE123 pKa = 4.18 RR124 pKa = 11.84 EE125 pKa = 3.86 LDD127 pKa = 4.5 LTLEE131 pKa = 4.87 DD132 pKa = 4.78 CDD134 pKa = 5.03 SLIPLDD140 pKa = 5.26 AITDD144 pKa = 3.71 HH145 pKa = 7.0 FLFLALPLLTEE156 pKa = 5.12 PDD158 pKa = 3.92 PDD160 pKa = 4.51 LDD162 pKa = 4.19 PEE164 pKa = 4.73 PEE166 pKa = 4.37 LEE168 pKa = 4.89 LEE170 pKa = 4.59 PEE172 pKa = 4.14 RR173 pKa = 11.84 QLEE176 pKa = 4.24 PEE178 pKa = 4.1 RR179 pKa = 11.84 QLEE182 pKa = 4.3 PEE184 pKa = 4.59 LEE186 pKa = 4.21 LEE188 pKa = 4.61 PEE190 pKa = 4.39 LEE192 pKa = 4.52 LDD194 pKa = 4.83 LTLEE198 pKa = 4.73 DD199 pKa = 4.64 CGSLIPLDD207 pKa = 5.06 AITDD211 pKa = 3.67 RR212 pKa = 11.84 RR213 pKa = 11.84 GGG215 pKa = 3.46
Molecular weight: 24.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.668
IPC_protein 3.656
Toseland 3.465
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.516
Rodwell 3.49
Grimsley 3.376
Solomon 3.617
Lehninger 3.567
Nozaki 3.732
DTASelect 3.897
Thurlkill 3.49
EMBOSS 3.528
Sillero 3.77
Patrickios 0.871
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.71
Protein with the highest isoelectric point:
>tr|A0A232F0I2|A0A232F0I2_9HYME SMC_N domain-containing protein OS=Trichomalopsis sarcophagae OX=543379 GN=TSAR_000364 PE=3 SV=1
MM1 pKa = 7.55 LSLHH5 pKa = 6.55 GSRR8 pKa = 11.84 SGQHH12 pKa = 6.22 APNKK16 pKa = 10.04 LRR18 pKa = 11.84 LSKK21 pKa = 10.3 PNRR24 pKa = 11.84 VRR26 pKa = 11.84 AGKK29 pKa = 10.04 SRR31 pKa = 11.84 LFLAPLSLQLPGVSTRR47 pKa = 11.84 GRR49 pKa = 11.84 GKK51 pKa = 10.64 VILIVSTQVATHH63 pKa = 5.13 TTRR66 pKa = 11.84 PTRR69 pKa = 11.84 HH70 pKa = 5.62 FAA72 pKa = 3.72
Molecular weight: 7.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.155
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.574
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.31
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.145
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16054
10
16064
6836071
8
7979
425.6
48.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.279 ± 0.022
1.98 ± 0.018
5.481 ± 0.015
6.772 ± 0.024
3.834 ± 0.016
5.121 ± 0.025
2.397 ± 0.009
5.897 ± 0.02
6.863 ± 0.025
9.187 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.259 ± 0.01
5.227 ± 0.019
4.794 ± 0.021
4.223 ± 0.021
5.463 ± 0.016
8.182 ± 0.026
5.66 ± 0.017
6.086 ± 0.017
1.052 ± 0.008
3.216 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here