Anaerococcus octavius
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1795 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I1M9U8|A0A2I1M9U8_9FIRM Type I-E CRISPR-associated protein Cse1/CasA OS=Anaerococcus octavius OX=54007 GN=CYJ34_03465 PE=4 SV=1
MM1 pKa = 7.01 TEE3 pKa = 3.98 KK4 pKa = 10.37 IEE6 pKa = 4.11 LHH8 pKa = 4.46 GHH10 pKa = 4.71 EE11 pKa = 6.11 LEE13 pKa = 4.16 FQKK16 pKa = 11.18 NSGKK20 pKa = 10.43 AVIEE24 pKa = 3.89 IDD26 pKa = 3.32 LGEE29 pKa = 4.42 VSDD32 pKa = 3.83 EE33 pKa = 4.47 CYY35 pKa = 10.58 LVDD38 pKa = 3.86 VFSVDD43 pKa = 2.97 GTDD46 pKa = 3.58 YY47 pKa = 11.16 VALISSEE54 pKa = 4.17 SNEE57 pKa = 3.69 IYY59 pKa = 10.23 IFYY62 pKa = 10.85 YY63 pKa = 10.38 EE64 pKa = 5.05 DD65 pKa = 3.34 SFEE68 pKa = 4.46 NDD70 pKa = 3.4 EE71 pKa = 4.4 IDD73 pKa = 4.44 LKK75 pKa = 11.52 VVDD78 pKa = 5.39 DD79 pKa = 4.44 EE80 pKa = 4.96 EE81 pKa = 4.42 EE82 pKa = 4.14 LDD84 pKa = 3.89 EE85 pKa = 4.41 VFHH88 pKa = 7.06 LFTHH92 pKa = 5.82 YY93 pKa = 10.33 WDD95 pKa = 5.49 DD96 pKa = 3.59 EE97 pKa = 4.55 SLDD100 pKa = 4.04 KK101 pKa = 11.4 LVDD104 pKa = 4.47 DD105 pKa = 4.42 YY106 pKa = 12.17 DD107 pKa = 4.23 NDD109 pKa = 3.8 IEE111 pKa = 5.18 HH112 pKa = 6.76 FADD115 pKa = 4.34 DD116 pKa = 4.01 EE117 pKa = 4.72 QVIEE121 pKa = 5.63 DD122 pKa = 4.47 NDD124 pKa = 4.25 TLDD127 pKa = 3.49 EE128 pKa = 4.26
Molecular weight: 15.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.554
IPC_protein 3.554
Toseland 3.35
ProMoST 3.706
Dawson 3.554
Bjellqvist 3.732
Wikipedia 3.478
Rodwell 3.389
Grimsley 3.261
Solomon 3.541
Lehninger 3.49
Nozaki 3.668
DTASelect 3.872
Thurlkill 3.401
EMBOSS 3.49
Sillero 3.681
Patrickios 0.731
IPC_peptide 3.528
IPC2_peptide 3.656
IPC2.peptide.svr19 3.678
Protein with the highest isoelectric point:
>tr|A0A2I1MC08|A0A2I1MC08_9FIRM R3H domain-containing protein OS=Anaerococcus octavius OX=54007 GN=CYJ34_02360 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.21 QPNRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.77 RR12 pKa = 11.84 AKK14 pKa = 10.12 DD15 pKa = 2.74 HH16 pKa = 6.15 GFRR19 pKa = 11.84 KK20 pKa = 9.99 RR21 pKa = 11.84 MSSPGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLKK32 pKa = 10.55 ARR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.13 KK37 pKa = 9.79 NRR39 pKa = 11.84 KK40 pKa = 8.94 RR41 pKa = 11.84 LSAA44 pKa = 4.01
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.906
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.252
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.974
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.058
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1795
0
1795
584478
30
3976
325.6
36.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.892 ± 0.054
0.687 ± 0.018
6.951 ± 0.054
7.529 ± 0.076
4.464 ± 0.048
5.964 ± 0.058
1.295 ± 0.019
9.456 ± 0.072
8.963 ± 0.069
9.05 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.433 ± 0.028
6.582 ± 0.056
2.73 ± 0.03
2.566 ± 0.03
3.398 ± 0.041
6.342 ± 0.043
4.892 ± 0.037
5.857 ± 0.044
0.59 ± 0.016
4.36 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here