Enterococcus phage EFRM31

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7PQ37|D7PQ37_9CAUD HNH endonuclease family protein OS=Enterococcus phage EFRM31 OX=767806 GN=gp11 PE=4 SV=1
MM1 pKa = 7.09GRR3 pKa = 11.84EE4 pKa = 4.18ANFSRR9 pKa = 11.84EE10 pKa = 3.77VVKK13 pKa = 10.59YY14 pKa = 9.86LKK16 pKa = 10.72SRR18 pKa = 11.84GALVNVNTANIYY30 pKa = 10.71DD31 pKa = 3.72RR32 pKa = 11.84VGRR35 pKa = 11.84ADD37 pKa = 4.42IEE39 pKa = 4.19ACYY42 pKa = 9.8KK43 pKa = 10.35GYY45 pKa = 11.0YY46 pKa = 8.68IALEE50 pKa = 4.34LKK52 pKa = 8.93TGNYY56 pKa = 9.55KK57 pKa = 10.31ADD59 pKa = 3.65KK60 pKa = 10.38LQIRR64 pKa = 11.84YY65 pKa = 8.61LQEE68 pKa = 3.32IRR70 pKa = 11.84CAGGYY75 pKa = 10.39GLLLRR80 pKa = 11.84DD81 pKa = 4.03TLDD84 pKa = 3.91DD85 pKa = 5.3LEE87 pKa = 6.23DD88 pKa = 4.2LLLHH92 pKa = 7.0LDD94 pKa = 3.47QMDD97 pKa = 3.78NGVEE101 pKa = 3.9YY102 pKa = 8.96PYY104 pKa = 10.37TYY106 pKa = 9.72EE107 pKa = 4.68QPDD110 pKa = 4.01LPEE113 pKa = 4.81INYY116 pKa = 10.52DD117 pKa = 3.76EE118 pKa = 6.04LEE120 pKa = 3.93IDD122 pKa = 3.54YY123 pKa = 10.78DD124 pKa = 3.71

Molecular weight: 14.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7PQ36|D7PQ36_9CAUD Uncharacterized protein gp10 OS=Enterococcus phage EFRM31 OX=767806 GN=gp10 PE=4 SV=1
MM1 pKa = 8.13DD2 pKa = 4.27FQIGTVKK9 pKa = 9.34KK10 pKa = 8.56TQGQVKK16 pKa = 9.66KK17 pKa = 10.05HH18 pKa = 4.88ITQCTTQEE26 pKa = 3.93KK27 pKa = 9.62EE28 pKa = 4.17QLNKK32 pKa = 10.86AKK34 pKa = 10.8DD35 pKa = 3.59RR36 pKa = 11.84VKK38 pKa = 10.88DD39 pKa = 3.37LWLKK43 pKa = 10.72RR44 pKa = 11.84GIKK47 pKa = 8.78TGYY50 pKa = 9.53HH51 pKa = 5.54LQDD54 pKa = 4.08KK55 pKa = 9.82IRR57 pKa = 11.84NGEE60 pKa = 4.11TKK62 pKa = 10.55FSYY65 pKa = 10.11LRR67 pKa = 11.84AMEE70 pKa = 4.26AMIHH74 pKa = 5.99GEE76 pKa = 3.62IIEE79 pKa = 4.15YY80 pKa = 10.93NEE82 pKa = 3.61TGADD86 pKa = 3.21KK87 pKa = 10.73RR88 pKa = 11.84VLIRR92 pKa = 11.84SGIGHH97 pKa = 6.27VGEE100 pKa = 5.09SIDD103 pKa = 5.52DD104 pKa = 3.59IVVQCIVVSLFSGKK118 pKa = 10.65VITSYY123 pKa = 11.27LNKK126 pKa = 10.42ASDD129 pKa = 3.26RR130 pKa = 11.84HH131 pKa = 5.24KK132 pKa = 9.68TLDD135 pKa = 3.18PRR137 pKa = 11.84RR138 pKa = 11.84YY139 pKa = 10.16NKK141 pKa = 9.7NLKK144 pKa = 10.04INLPKK149 pKa = 10.55HH150 pKa = 4.47LTKK153 pKa = 10.86

Molecular weight: 17.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23

0

23

4184

62

574

181.9

20.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.856 ± 0.587

0.86 ± 0.195

6.31 ± 0.383

8.7 ± 0.65

3.657 ± 0.34

5.378 ± 0.526

1.769 ± 0.277

6.573 ± 0.513

8.748 ± 0.495

8.317 ± 0.593

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.17

5.641 ± 0.607

3.25 ± 0.367

4.23 ± 0.303

4.207 ± 0.326

5.402 ± 0.4

6.597 ± 0.612

6.644 ± 0.476

0.932 ± 0.156

4.183 ± 0.43
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski