Amino acid dipepetide frequency for Enterococcus phage EFRM31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.717AlaAla: 0.717 ± 0.451
0.478AlaCys: 0.478 ± 0.416
4.303AlaAsp: 4.303 ± 1.034
4.303AlaGlu: 4.303 ± 0.983
2.152AlaPhe: 2.152 ± 0.63
1.913AlaGly: 1.913 ± 0.616
0.717AlaHis: 0.717 ± 0.419
3.586AlaIle: 3.586 ± 0.903
5.977AlaLys: 5.977 ± 1.046
6.455AlaLeu: 6.455 ± 0.904
4.303AlaMet: 4.303 ± 0.994
4.064AlaAsn: 4.064 ± 1.007
1.673AlaPro: 1.673 ± 0.545
1.434AlaGln: 1.434 ± 0.453
1.673AlaArg: 1.673 ± 0.533
4.781AlaSer: 4.781 ± 0.878
3.825AlaThr: 3.825 ± 0.955
4.303AlaVal: 4.303 ± 1.222
0.239AlaTrp: 0.239 ± 0.199
1.673AlaTyr: 1.673 ± 0.418
0.0AlaXaa: 0.0 ± 0.0
Cys
0.239CysAla: 0.239 ± 0.296
0.0CysCys: 0.0 ± 0.0
0.239CysAsp: 0.239 ± 0.23
1.195CysGlu: 1.195 ± 0.629
0.0CysPhe: 0.0 ± 0.0
0.478CysGly: 0.478 ± 0.314
0.478CysHis: 0.478 ± 0.322
0.239CysIle: 0.239 ± 0.272
0.956CysLys: 0.956 ± 0.523
0.717CysLeu: 0.717 ± 0.357
0.0CysMet: 0.0 ± 0.0
0.717CysAsn: 0.717 ± 0.489
0.0CysPro: 0.0 ± 0.0
0.478CysGln: 0.478 ± 0.324
0.239CysArg: 0.239 ± 0.258
0.478CysSer: 0.478 ± 0.317
0.956CysThr: 0.956 ± 0.453
0.717CysVal: 0.717 ± 0.437
0.239CysTrp: 0.239 ± 0.234
0.239CysTyr: 0.239 ± 0.296
0.0CysXaa: 0.0 ± 0.0
Asp
3.347AspAla: 3.347 ± 0.914
0.478AspCys: 0.478 ± 0.289
3.586AspAsp: 3.586 ± 0.982
3.825AspGlu: 3.825 ± 1.12
3.108AspPhe: 3.108 ± 0.7
4.542AspGly: 4.542 ± 0.898
2.152AspHis: 2.152 ± 0.771
4.542AspIle: 4.542 ± 1.137
5.977AspLys: 5.977 ± 1.083
5.977AspLeu: 5.977 ± 1.235
1.913AspMet: 1.913 ± 0.794
4.064AspAsn: 4.064 ± 0.892
2.63AspPro: 2.63 ± 1.024
0.956AspGln: 0.956 ± 0.349
2.152AspArg: 2.152 ± 0.773
3.586AspSer: 3.586 ± 0.746
3.825AspThr: 3.825 ± 1.077
2.63AspVal: 2.63 ± 0.745
0.239AspTrp: 0.239 ± 0.237
3.586AspTyr: 3.586 ± 1.021
0.0AspXaa: 0.0 ± 0.0
Glu
5.738GluAla: 5.738 ± 1.442
0.717GluCys: 0.717 ± 0.413
5.259GluAsp: 5.259 ± 0.846
9.563GluGlu: 9.563 ± 3.563
2.869GluPhe: 2.869 ± 0.762
4.542GluGly: 4.542 ± 0.938
1.673GluHis: 1.673 ± 0.651
4.064GluIle: 4.064 ± 1.311
5.738GluLys: 5.738 ± 1.063
7.411GluLeu: 7.411 ± 1.289
1.434GluMet: 1.434 ± 0.758
3.347GluAsn: 3.347 ± 0.814
3.586GluPro: 3.586 ± 1.355
4.542GluGln: 4.542 ± 1.205
2.869GluArg: 2.869 ± 0.671
3.825GluSer: 3.825 ± 1.01
5.02GluThr: 5.02 ± 1.232
7.411GluVal: 7.411 ± 1.34
2.63GluTrp: 2.63 ± 0.73
4.781GluTyr: 4.781 ± 1.08
0.0GluXaa: 0.0 ± 0.0
Phe
1.434PheAla: 1.434 ± 0.395
0.0PheCys: 0.0 ± 0.0
2.391PheAsp: 2.391 ± 0.776
2.869PheGlu: 2.869 ± 0.908
0.956PhePhe: 0.956 ± 0.519
2.391PheGly: 2.391 ± 0.513
0.0PheHis: 0.0 ± 0.0
5.259PheIle: 5.259 ± 1.364
2.63PheLys: 2.63 ± 0.749
1.673PheLeu: 1.673 ± 0.564
1.195PheMet: 1.195 ± 0.535
2.152PheAsn: 2.152 ± 0.547
0.717PhePro: 0.717 ± 0.342
1.913PheGln: 1.913 ± 0.505
1.434PheArg: 1.434 ± 0.467
1.913PheSer: 1.913 ± 0.7
3.825PheThr: 3.825 ± 1.122
2.152PheVal: 2.152 ± 0.816
0.717PheTrp: 0.717 ± 0.317
0.956PheTyr: 0.956 ± 0.485
0.0PheXaa: 0.0 ± 0.0
Gly
1.913GlyAla: 1.913 ± 0.609
0.0GlyCys: 0.0 ± 0.0
2.391GlyAsp: 2.391 ± 0.466
5.259GlyGlu: 5.259 ± 1.047
3.108GlyPhe: 3.108 ± 0.723
2.869GlyGly: 2.869 ± 1.044
0.717GlyHis: 0.717 ± 0.394
4.064GlyIle: 4.064 ± 0.994
5.02GlyLys: 5.02 ± 0.954
5.259GlyLeu: 5.259 ± 1.153
1.434GlyMet: 1.434 ± 0.638
2.63GlyAsn: 2.63 ± 0.767
0.478GlyPro: 0.478 ± 0.26
2.63GlyGln: 2.63 ± 0.672
3.825GlyArg: 3.825 ± 0.977
1.673GlySer: 1.673 ± 0.783
3.586GlyThr: 3.586 ± 0.751
3.586GlyVal: 3.586 ± 1.125
0.717GlyTrp: 0.717 ± 0.48
2.63GlyTyr: 2.63 ± 1.11
0.0GlyXaa: 0.0 ± 0.0
His
0.717HisAla: 0.717 ± 0.436
0.239HisCys: 0.239 ± 0.258
0.717HisAsp: 0.717 ± 0.337
0.956HisGlu: 0.956 ± 0.713
0.956HisPhe: 0.956 ± 0.456
0.956HisGly: 0.956 ± 0.444
0.239HisHis: 0.239 ± 0.258
1.913HisIle: 1.913 ± 0.54
1.195HisLys: 1.195 ± 0.559
0.956HisLeu: 0.956 ± 0.599
0.478HisMet: 0.478 ± 0.269
0.956HisAsn: 0.956 ± 0.449
0.478HisPro: 0.478 ± 0.376
0.956HisGln: 0.956 ± 0.38
1.913HisArg: 1.913 ± 1.108
0.956HisSer: 0.956 ± 0.53
0.956HisThr: 0.956 ± 0.673
1.195HisVal: 1.195 ± 0.476
0.0HisTrp: 0.0 ± 0.0
0.956HisTyr: 0.956 ± 0.444
0.0HisXaa: 0.0 ± 0.0
Ile
3.825IleAla: 3.825 ± 0.837
0.956IleCys: 0.956 ± 0.476
4.781IleAsp: 4.781 ± 1.14
7.172IleGlu: 7.172 ± 1.466
1.434IlePhe: 1.434 ± 0.63
2.391IleGly: 2.391 ± 0.665
0.956IleHis: 0.956 ± 0.503
4.064IleIle: 4.064 ± 0.671
5.738IleLys: 5.738 ± 0.876
5.02IleLeu: 5.02 ± 1.152
1.195IleMet: 1.195 ± 0.585
4.064IleAsn: 4.064 ± 0.908
3.347IlePro: 3.347 ± 0.625
2.869IleGln: 2.869 ± 0.7
2.869IleArg: 2.869 ± 0.847
3.825IleSer: 3.825 ± 1.011
3.586IleThr: 3.586 ± 0.826
5.02IleVal: 5.02 ± 0.877
0.717IleTrp: 0.717 ± 0.415
1.913IleTyr: 1.913 ± 0.686
0.0IleXaa: 0.0 ± 0.0
Lys
6.933LysAla: 6.933 ± 1.446
0.239LysCys: 0.239 ± 0.23
5.259LysAsp: 5.259 ± 1.159
9.323LysGlu: 9.323 ± 1.754
3.586LysPhe: 3.586 ± 0.858
4.303LysGly: 4.303 ± 0.897
1.434LysHis: 1.434 ± 0.718
4.303LysIle: 4.303 ± 0.952
5.02LysLys: 5.02 ± 0.964
7.65LysLeu: 7.65 ± 1.736
3.347LysMet: 3.347 ± 0.879
4.064LysAsn: 4.064 ± 0.897
2.152LysPro: 2.152 ± 0.471
5.02LysGln: 5.02 ± 0.966
4.542LysArg: 4.542 ± 1.165
4.303LysSer: 4.303 ± 1.052
6.694LysThr: 6.694 ± 1.227
6.216LysVal: 6.216 ± 0.921
0.0LysTrp: 0.0 ± 0.0
3.108LysTyr: 3.108 ± 0.91
0.0LysXaa: 0.0 ± 0.0
Leu
3.586LeuAla: 3.586 ± 0.94
0.956LeuCys: 0.956 ± 0.546
5.977LeuAsp: 5.977 ± 1.138
8.845LeuGlu: 8.845 ± 1.397
1.913LeuPhe: 1.913 ± 0.664
5.738LeuGly: 5.738 ± 1.462
1.195LeuHis: 1.195 ± 0.48
5.02LeuIle: 5.02 ± 1.181
8.367LeuLys: 8.367 ± 1.419
7.411LeuLeu: 7.411 ± 2.115
1.195LeuMet: 1.195 ± 0.477
4.542LeuAsn: 4.542 ± 0.828
3.825LeuPro: 3.825 ± 1.05
3.586LeuGln: 3.586 ± 1.136
3.825LeuArg: 3.825 ± 0.755
5.259LeuSer: 5.259 ± 0.822
3.586LeuThr: 3.586 ± 0.598
4.781LeuVal: 4.781 ± 1.618
0.956LeuTrp: 0.956 ± 0.511
2.63LeuTyr: 2.63 ± 0.772
0.0LeuXaa: 0.0 ± 0.0
Met
1.673MetAla: 1.673 ± 0.637
0.478MetCys: 0.478 ± 0.306
1.195MetAsp: 1.195 ± 0.461
2.391MetGlu: 2.391 ± 0.76
0.717MetPhe: 0.717 ± 0.407
1.913MetGly: 1.913 ± 0.746
0.478MetHis: 0.478 ± 0.318
2.152MetIle: 2.152 ± 0.649
1.434MetLys: 1.434 ± 0.576
1.913MetLeu: 1.913 ± 0.569
0.478MetMet: 0.478 ± 0.295
1.913MetAsn: 1.913 ± 0.747
1.195MetPro: 1.195 ± 0.504
0.717MetGln: 0.717 ± 0.394
1.913MetArg: 1.913 ± 0.775
2.152MetSer: 2.152 ± 0.637
1.195MetThr: 1.195 ± 0.514
1.673MetVal: 1.673 ± 0.803
0.478MetTrp: 0.478 ± 0.34
1.434MetTyr: 1.434 ± 0.584
0.0MetXaa: 0.0 ± 0.0
Asn
4.064AsnAla: 4.064 ± 1.366
0.239AsnCys: 0.239 ± 0.267
2.391AsnAsp: 2.391 ± 0.882
3.825AsnGlu: 3.825 ± 0.871
1.913AsnPhe: 1.913 ± 0.523
4.542AsnGly: 4.542 ± 0.959
1.673AsnHis: 1.673 ± 0.869
3.825AsnIle: 3.825 ± 1.023
6.933AsnLys: 6.933 ± 1.014
4.542AsnLeu: 4.542 ± 1.069
2.152AsnMet: 2.152 ± 0.63
2.869AsnAsn: 2.869 ± 0.787
2.152AsnPro: 2.152 ± 0.508
0.717AsnGln: 0.717 ± 0.436
0.956AsnArg: 0.956 ± 0.395
2.391AsnSer: 2.391 ± 1.129
4.781AsnThr: 4.781 ± 1.001
3.586AsnVal: 3.586 ± 1.117
0.717AsnTrp: 0.717 ± 0.438
2.152AsnTyr: 2.152 ± 0.858
0.0AsnXaa: 0.0 ± 0.0
Pro
1.673ProAla: 1.673 ± 0.704
0.0ProCys: 0.0 ± 0.0
2.63ProAsp: 2.63 ± 0.952
3.825ProGlu: 3.825 ± 1.051
0.956ProPhe: 0.956 ± 0.442
0.0ProGly: 0.0 ± 0.0
0.239ProHis: 0.239 ± 0.194
1.434ProIle: 1.434 ± 0.757
4.064ProLys: 4.064 ± 1.441
2.63ProLeu: 2.63 ± 0.996
0.956ProMet: 0.956 ± 0.44
2.391ProAsn: 2.391 ± 0.705
0.239ProPro: 0.239 ± 0.23
1.913ProGln: 1.913 ± 0.69
0.956ProArg: 0.956 ± 0.47
1.434ProSer: 1.434 ± 0.4
3.586ProThr: 3.586 ± 1.016
1.913ProVal: 1.913 ± 0.722
0.0ProTrp: 0.0 ± 0.0
1.434ProTyr: 1.434 ± 0.812
0.0ProXaa: 0.0 ± 0.0
Gln
2.63GlnAla: 2.63 ± 0.866
0.956GlnCys: 0.956 ± 0.631
2.391GlnAsp: 2.391 ± 0.524
1.913GlnGlu: 1.913 ± 0.75
1.195GlnPhe: 1.195 ± 0.469
2.391GlnGly: 2.391 ± 0.485
0.717GlnHis: 0.717 ± 0.368
3.108GlnIle: 3.108 ± 0.685
2.869GlnLys: 2.869 ± 0.727
3.586GlnLeu: 3.586 ± 0.925
0.717GlnMet: 0.717 ± 0.404
1.195GlnAsn: 1.195 ± 0.781
2.152GlnPro: 2.152 ± 0.573
3.347GlnGln: 3.347 ± 0.804
2.63GlnArg: 2.63 ± 0.734
2.152GlnSer: 2.152 ± 0.723
1.913GlnThr: 1.913 ± 0.56
3.825GlnVal: 3.825 ± 1.065
0.239GlnTrp: 0.239 ± 0.258
2.391GlnTyr: 2.391 ± 0.598
0.0GlnXaa: 0.0 ± 0.0
Arg
2.63ArgAla: 2.63 ± 0.683
0.717ArgCys: 0.717 ± 0.441
2.869ArgAsp: 2.869 ± 0.706
2.152ArgGlu: 2.152 ± 0.896
1.673ArgPhe: 1.673 ± 0.377
2.391ArgGly: 2.391 ± 0.903
1.195ArgHis: 1.195 ± 0.546
2.391ArgIle: 2.391 ± 0.68
4.064ArgLys: 4.064 ± 1.167
2.869ArgLeu: 2.869 ± 0.872
0.717ArgMet: 0.717 ± 0.337
2.152ArgAsn: 2.152 ± 0.597
1.673ArgPro: 1.673 ± 0.578
2.63ArgGln: 2.63 ± 0.669
2.152ArgArg: 2.152 ± 0.541
2.63ArgSer: 2.63 ± 0.869
1.195ArgThr: 1.195 ± 0.532
3.347ArgVal: 3.347 ± 1.034
0.717ArgTrp: 0.717 ± 0.325
1.913ArgTyr: 1.913 ± 0.767
0.0ArgXaa: 0.0 ± 0.0
Ser
3.347SerAla: 3.347 ± 1.146
0.239SerCys: 0.239 ± 0.24
2.63SerAsp: 2.63 ± 0.519
4.303SerGlu: 4.303 ± 0.763
2.869SerPhe: 2.869 ± 0.6
4.064SerGly: 4.064 ± 0.944
1.913SerHis: 1.913 ± 0.653
4.064SerIle: 4.064 ± 0.742
4.542SerLys: 4.542 ± 1.059
5.259SerLeu: 5.259 ± 0.96
0.717SerMet: 0.717 ± 0.358
2.391SerAsn: 2.391 ± 0.642
1.195SerPro: 1.195 ± 0.471
3.347SerGln: 3.347 ± 1.084
1.913SerArg: 1.913 ± 0.778
2.391SerSer: 2.391 ± 0.725
3.347SerThr: 3.347 ± 1.522
2.391SerVal: 2.391 ± 0.666
0.478SerTrp: 0.478 ± 0.468
2.63SerTyr: 2.63 ± 1.205
0.0SerXaa: 0.0 ± 0.0
Thr
4.781ThrAla: 4.781 ± 1.354
0.239ThrCys: 0.239 ± 0.24
3.586ThrAsp: 3.586 ± 0.751
5.02ThrGlu: 5.02 ± 1.477
1.434ThrPhe: 1.434 ± 0.744
3.825ThrGly: 3.825 ± 1.129
0.717ThrHis: 0.717 ± 0.415
4.064ThrIle: 4.064 ± 1.026
6.933ThrLys: 6.933 ± 1.263
5.498ThrLeu: 5.498 ± 1.435
2.391ThrMet: 2.391 ± 0.654
5.259ThrAsn: 5.259 ± 1.198
1.913ThrPro: 1.913 ± 0.494
2.869ThrGln: 2.869 ± 1.019
1.673ThrArg: 1.673 ± 0.672
2.63ThrSer: 2.63 ± 1.032
6.455ThrThr: 6.455 ± 1.551
4.303ThrVal: 4.303 ± 1.097
0.0ThrTrp: 0.0 ± 0.0
2.391ThrTyr: 2.391 ± 0.79
0.0ThrXaa: 0.0 ± 0.0
Val
5.977ValAla: 5.977 ± 1.068
0.478ValCys: 0.478 ± 0.346
6.455ValAsp: 6.455 ± 1.321
4.542ValGlu: 4.542 ± 1.112
3.347ValPhe: 3.347 ± 0.837
2.869ValGly: 2.869 ± 0.842
0.956ValHis: 0.956 ± 0.495
3.108ValIle: 3.108 ± 0.739
5.498ValLys: 5.498 ± 0.986
4.542ValLeu: 4.542 ± 0.867
1.673ValMet: 1.673 ± 0.478
4.542ValAsn: 4.542 ± 1.053
1.913ValPro: 1.913 ± 0.878
1.673ValGln: 1.673 ± 0.819
2.152ValArg: 2.152 ± 0.925
5.498ValSer: 5.498 ± 1.195
4.542ValThr: 4.542 ± 1.418
3.825ValVal: 3.825 ± 0.71
0.0ValTrp: 0.0 ± 0.0
2.869ValTyr: 2.869 ± 0.826
0.0ValXaa: 0.0 ± 0.0
Trp
0.239TrpAla: 0.239 ± 0.233
0.478TrpCys: 0.478 ± 0.382
0.0TrpAsp: 0.0 ± 0.0
1.434TrpGlu: 1.434 ± 0.51
0.478TrpPhe: 0.478 ± 0.371
0.239TrpGly: 0.239 ± 0.293
0.0TrpHis: 0.0 ± 0.0
0.956TrpIle: 0.956 ± 0.512
0.478TrpLys: 0.478 ± 0.307
0.717TrpLeu: 0.717 ± 0.383
0.239TrpMet: 0.239 ± 0.199
0.0TrpAsn: 0.0 ± 0.0
0.0TrpPro: 0.0 ± 0.0
0.239TrpGln: 0.239 ± 0.194
0.956TrpArg: 0.956 ± 0.415
0.478TrpSer: 0.478 ± 0.333
0.478TrpThr: 0.478 ± 0.272
1.434TrpVal: 1.434 ± 0.503
0.239TrpTrp: 0.239 ± 0.218
0.239TrpTyr: 0.239 ± 0.259
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.108TyrAla: 3.108 ± 0.9
0.717TyrCys: 0.717 ± 0.383
4.064TyrAsp: 4.064 ± 1.139
4.303TyrGlu: 4.303 ± 0.771
1.913TyrPhe: 1.913 ± 0.784
1.434TyrGly: 1.434 ± 0.569
0.239TyrHis: 0.239 ± 0.272
3.586TyrIle: 3.586 ± 0.845
4.064TyrLys: 4.064 ± 1.206
3.108TyrLeu: 3.108 ± 0.82
0.717TyrMet: 0.717 ± 0.369
3.108TyrAsn: 3.108 ± 0.822
1.195TyrPro: 1.195 ± 0.711
0.478TyrGln: 0.478 ± 0.307
1.434TyrArg: 1.434 ± 0.611
1.673TyrSer: 1.673 ± 0.921
2.63TyrThr: 2.63 ± 0.932
2.152TyrVal: 2.152 ± 0.576
0.0TyrTrp: 0.0 ± 0.0
1.913TyrTyr: 1.913 ± 0.801
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 23 proteins (4184 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski