Nitrincola phage 1M3-16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; unclassified Caudovirales

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 144 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023NGS7|A0A023NGS7_9CAUD NADAR domain-containing protein OS=Nitrincola phage 1M3-16 OX=1472912 GN=M316_0088 PE=4 SV=1
MM1 pKa = 6.65THH3 pKa = 6.46SLALIVEE10 pKa = 4.57VEE12 pKa = 4.35TTSYY16 pKa = 11.72DD17 pKa = 3.49CLTVEE22 pKa = 5.46KK23 pKa = 10.88VIDD26 pKa = 5.26LINNPQDD33 pKa = 4.0EE34 pKa = 4.9DD35 pKa = 4.42DD36 pKa = 4.06SDD38 pKa = 4.46YY39 pKa = 11.65AQHH42 pKa = 6.71PCNRR46 pKa = 11.84VWDD49 pKa = 3.96RR50 pKa = 11.84TEE52 pKa = 4.56DD53 pKa = 3.68QVCLLRR59 pKa = 11.84GII61 pKa = 4.54

Molecular weight: 7.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023NH00|A0A023NH00_9CAUD Uncharacterized protein OS=Nitrincola phage 1M3-16 OX=1472912 GN=M316_0082 PE=4 SV=1
MM1 pKa = 6.83HH2 pKa = 7.42TNTGFEE8 pKa = 4.0RR9 pKa = 11.84SEE11 pKa = 4.44KK12 pKa = 8.69PMKK15 pKa = 9.71MKK17 pKa = 10.16QRR19 pKa = 11.84EE20 pKa = 3.86YY21 pKa = 10.42ADD23 pKa = 3.5RR24 pKa = 11.84YY25 pKa = 8.06EE26 pKa = 4.44RR27 pKa = 11.84KK28 pKa = 9.39VSKK31 pKa = 10.52KK32 pKa = 8.64MRR34 pKa = 11.84DD35 pKa = 3.02RR36 pKa = 11.84KK37 pKa = 9.93RR38 pKa = 11.84SKK40 pKa = 10.65KK41 pKa = 10.57GDD43 pKa = 3.2WAA45 pKa = 4.81

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

144

0

144

24094

30

1472

167.3

19.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.943 ± 0.418

1.419 ± 0.16

6.475 ± 0.201

7.919 ± 0.305

3.715 ± 0.14

6.753 ± 0.235

1.938 ± 0.154

6.595 ± 0.19

6.537 ± 0.419

8.284 ± 0.192

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.814 ± 0.152

4.752 ± 0.148

3.383 ± 0.197

3.594 ± 0.234

4.914 ± 0.177

6.458 ± 0.191

5.815 ± 0.3

6.865 ± 0.263

1.602 ± 0.103

4.225 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski