Amino acid dipepetide frequency for Nitrincola phage 1M3-16

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.69AlaAla: 4.69 ± 0.977
0.706AlaCys: 0.706 ± 0.191
4.068AlaAsp: 4.068 ± 0.472
4.4AlaGlu: 4.4 ± 0.615
2.158AlaPhe: 2.158 ± 0.348
3.611AlaGly: 3.611 ± 0.568
0.996AlaHis: 0.996 ± 0.227
4.69AlaIle: 4.69 ± 0.45
3.486AlaLys: 3.486 ± 0.419
4.607AlaLeu: 4.607 ± 0.53
2.2AlaMet: 2.2 ± 0.551
2.781AlaAsn: 2.781 ± 0.412
1.453AlaPro: 1.453 ± 0.228
2.449AlaGln: 2.449 ± 0.605
2.366AlaArg: 2.366 ± 0.328
3.694AlaSer: 3.694 ± 0.441
3.403AlaThr: 3.403 ± 0.564
4.109AlaVal: 4.109 ± 0.399
1.121AlaTrp: 1.121 ± 0.209
2.449AlaTyr: 2.449 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
0.83CysAla: 0.83 ± 0.183
0.249CysCys: 0.249 ± 0.11
0.374CysAsp: 0.374 ± 0.135
0.996CysGlu: 0.996 ± 0.23
0.706CysPhe: 0.706 ± 0.187
0.996CysGly: 0.996 ± 0.227
0.166CysHis: 0.166 ± 0.086
0.747CysIle: 0.747 ± 0.172
0.996CysLys: 0.996 ± 0.258
1.162CysLeu: 1.162 ± 0.21
0.457CysMet: 0.457 ± 0.146
0.83CysAsn: 0.83 ± 0.2
0.332CysPro: 0.332 ± 0.112
0.457CysGln: 0.457 ± 0.149
0.581CysArg: 0.581 ± 0.157
0.83CysSer: 0.83 ± 0.193
0.83CysThr: 0.83 ± 0.203
1.204CysVal: 1.204 ± 0.237
0.623CysTrp: 0.623 ± 0.167
0.83CysTyr: 0.83 ± 0.207
0.0CysXaa: 0.0 ± 0.0
Asp
3.902AspAla: 3.902 ± 0.457
0.83AspCys: 0.83 ± 0.171
3.902AspAsp: 3.902 ± 0.557
4.441AspGlu: 4.441 ± 0.405
2.324AspPhe: 2.324 ± 0.39
4.898AspGly: 4.898 ± 0.458
0.83AspHis: 0.83 ± 0.165
4.856AspIle: 4.856 ± 0.464
3.985AspLys: 3.985 ± 0.513
5.769AspLeu: 5.769 ± 0.491
1.411AspMet: 1.411 ± 0.266
3.03AspAsn: 3.03 ± 0.296
2.324AspPro: 2.324 ± 0.307
2.49AspGln: 2.49 ± 0.353
2.573AspArg: 2.573 ± 0.317
4.275AspSer: 4.275 ± 0.46
3.32AspThr: 3.32 ± 0.431
4.773AspVal: 4.773 ± 0.5
1.411AspTrp: 1.411 ± 0.285
3.403AspTyr: 3.403 ± 0.415
0.0AspXaa: 0.0 ± 0.0
Glu
5.022GluAla: 5.022 ± 0.709
0.913GluCys: 0.913 ± 0.204
5.396GluAsp: 5.396 ± 0.466
7.181GluGlu: 7.181 ± 0.809
3.445GluPhe: 3.445 ± 0.335
5.437GluGly: 5.437 ± 0.349
1.951GluHis: 1.951 ± 0.251
4.732GluIle: 4.732 ± 0.365
3.736GluLys: 3.736 ± 0.456
6.931GluLeu: 6.931 ± 0.505
2.656GluMet: 2.656 ± 0.398
3.445GluAsn: 3.445 ± 0.353
1.619GluPro: 1.619 ± 0.289
2.864GluGln: 2.864 ± 0.443
4.068GluArg: 4.068 ± 0.426
4.109GluSer: 4.109 ± 0.391
4.109GluThr: 4.109 ± 0.409
5.935GluVal: 5.935 ± 0.539
1.536GluTrp: 1.536 ± 0.284
4.109GluTyr: 4.109 ± 0.361
0.0GluXaa: 0.0 ± 0.0
Phe
1.785PheAla: 1.785 ± 0.296
0.664PheCys: 0.664 ± 0.168
2.656PheAsp: 2.656 ± 0.303
3.03PheGlu: 3.03 ± 0.365
0.913PhePhe: 0.913 ± 0.206
2.117PheGly: 2.117 ± 0.296
0.706PheHis: 0.706 ± 0.205
2.573PheIle: 2.573 ± 0.351
2.905PheLys: 2.905 ± 0.395
3.32PheLeu: 3.32 ± 0.396
0.913PheMet: 0.913 ± 0.216
1.868PheAsn: 1.868 ± 0.294
1.743PhePro: 1.743 ± 0.271
1.204PheGln: 1.204 ± 0.226
1.743PheArg: 1.743 ± 0.3
2.49PheSer: 2.49 ± 0.297
2.366PheThr: 2.366 ± 0.284
1.868PheVal: 1.868 ± 0.258
0.789PheTrp: 0.789 ± 0.178
1.494PheTyr: 1.494 ± 0.269
0.0PheXaa: 0.0 ± 0.0
Gly
3.403GlyAla: 3.403 ± 0.53
1.453GlyCys: 1.453 ± 0.282
5.064GlyAsp: 5.064 ± 0.631
5.686GlyGlu: 5.686 ± 0.457
2.615GlyPhe: 2.615 ± 0.34
4.939GlyGly: 4.939 ± 0.465
1.204GlyHis: 1.204 ± 0.279
3.694GlyIle: 3.694 ± 0.36
4.358GlyLys: 4.358 ± 0.415
4.441GlyLeu: 4.441 ± 0.458
2.117GlyMet: 2.117 ± 0.274
3.57GlyAsn: 3.57 ± 0.42
0.83GlyPro: 0.83 ± 0.155
2.2GlyGln: 2.2 ± 0.337
4.234GlyArg: 4.234 ± 0.431
4.068GlySer: 4.068 ± 0.426
3.694GlyThr: 3.694 ± 0.451
5.603GlyVal: 5.603 ± 0.462
1.204GlyTrp: 1.204 ± 0.237
3.154GlyTyr: 3.154 ± 0.425
0.0GlyXaa: 0.0 ± 0.0
His
0.747HisAla: 0.747 ± 0.183
0.374HisCys: 0.374 ± 0.129
1.038HisAsp: 1.038 ± 0.199
1.536HisGlu: 1.536 ± 0.29
0.623HisPhe: 0.623 ± 0.136
1.37HisGly: 1.37 ± 0.263
0.706HisHis: 0.706 ± 0.188
1.37HisIle: 1.37 ± 0.216
1.494HisLys: 1.494 ± 0.288
1.702HisLeu: 1.702 ± 0.242
0.249HisMet: 0.249 ± 0.083
0.623HisAsn: 0.623 ± 0.165
0.996HisPro: 0.996 ± 0.169
0.498HisGln: 0.498 ± 0.154
0.913HisArg: 0.913 ± 0.208
1.287HisSer: 1.287 ± 0.28
1.287HisThr: 1.287 ± 0.247
1.121HisVal: 1.121 ± 0.24
0.166HisTrp: 0.166 ± 0.079
1.287HisTyr: 1.287 ± 0.227
0.0HisXaa: 0.0 ± 0.0
Ile
4.358IleAla: 4.358 ± 0.566
0.789IleCys: 0.789 ± 0.205
3.943IleAsp: 3.943 ± 0.345
5.728IleGlu: 5.728 ± 0.46
2.656IlePhe: 2.656 ± 0.425
4.275IleGly: 4.275 ± 0.512
1.121IleHis: 1.121 ± 0.252
4.317IleIle: 4.317 ± 0.493
4.441IleLys: 4.441 ± 0.385
5.313IleLeu: 5.313 ± 0.526
1.245IleMet: 1.245 ± 0.263
3.362IleAsn: 3.362 ± 0.39
2.822IlePro: 2.822 ± 0.421
2.117IleGln: 2.117 ± 0.338
3.362IleArg: 3.362 ± 0.448
4.275IleSer: 4.275 ± 0.423
4.69IleThr: 4.69 ± 0.557
3.985IleVal: 3.985 ± 0.404
0.664IleTrp: 0.664 ± 0.149
2.49IleTyr: 2.49 ± 0.421
0.0IleXaa: 0.0 ± 0.0
Lys
3.777LysAla: 3.777 ± 0.493
0.498LysCys: 0.498 ± 0.153
4.607LysAsp: 4.607 ± 0.433
5.105LysGlu: 5.105 ± 0.623
2.117LysPhe: 2.117 ± 0.303
4.441LysGly: 4.441 ± 0.467
2.449LysHis: 2.449 ± 0.338
4.856LysIle: 4.856 ± 0.556
5.064LysLys: 5.064 ± 0.542
4.358LysLeu: 4.358 ± 0.396
2.615LysMet: 2.615 ± 0.379
2.324LysAsn: 2.324 ± 0.354
2.449LysPro: 2.449 ± 0.304
2.615LysGln: 2.615 ± 0.362
3.196LysArg: 3.196 ± 0.364
3.486LysSer: 3.486 ± 0.424
3.237LysThr: 3.237 ± 0.314
4.441LysVal: 4.441 ± 0.403
0.789LysTrp: 0.789 ± 0.188
2.947LysTyr: 2.947 ± 0.417
0.0LysXaa: 0.0 ± 0.0
Leu
4.939LeuAla: 4.939 ± 0.511
1.328LeuCys: 1.328 ± 0.269
4.69LeuAsp: 4.69 ± 0.492
7.637LeuGlu: 7.637 ± 0.74
3.154LeuPhe: 3.154 ± 0.339
5.105LeuGly: 5.105 ± 0.443
1.702LeuHis: 1.702 ± 0.285
5.147LeuIle: 5.147 ± 0.493
6.599LeuLys: 6.599 ± 0.665
6.267LeuLeu: 6.267 ± 0.557
2.075LeuMet: 2.075 ± 0.296
4.317LeuAsn: 4.317 ± 0.414
3.237LeuPro: 3.237 ± 0.375
3.03LeuGln: 3.03 ± 0.412
3.943LeuArg: 3.943 ± 0.476
5.064LeuSer: 5.064 ± 0.484
5.52LeuThr: 5.52 ± 0.487
5.188LeuVal: 5.188 ± 0.551
1.121LeuTrp: 1.121 ± 0.215
2.781LeuTyr: 2.781 ± 0.375
0.0LeuXaa: 0.0 ± 0.0
Met
1.702MetAla: 1.702 ± 0.31
0.581MetCys: 0.581 ± 0.191
0.996MetAsp: 0.996 ± 0.231
1.66MetGlu: 1.66 ± 0.265
0.913MetPhe: 0.913 ± 0.211
1.743MetGly: 1.743 ± 0.29
0.54MetHis: 0.54 ± 0.183
1.992MetIle: 1.992 ± 0.301
2.988MetLys: 2.988 ± 0.339
2.615MetLeu: 2.615 ± 0.338
0.706MetMet: 0.706 ± 0.181
1.619MetAsn: 1.619 ± 0.262
1.287MetPro: 1.287 ± 0.222
1.37MetGln: 1.37 ± 0.21
1.121MetArg: 1.121 ± 0.22
1.577MetSer: 1.577 ± 0.262
2.158MetThr: 2.158 ± 0.301
1.204MetVal: 1.204 ± 0.261
0.291MetTrp: 0.291 ± 0.104
1.079MetTyr: 1.079 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
2.905AsnAla: 2.905 ± 0.361
0.664AsnCys: 0.664 ± 0.181
2.988AsnAsp: 2.988 ± 0.327
2.739AsnGlu: 2.739 ± 0.331
1.785AsnPhe: 1.785 ± 0.266
3.03AsnGly: 3.03 ± 0.388
0.747AsnHis: 0.747 ± 0.204
3.611AsnIle: 3.611 ± 0.405
2.656AsnLys: 2.656 ± 0.296
3.943AsnLeu: 3.943 ± 0.486
1.66AsnMet: 1.66 ± 0.295
2.158AsnAsn: 2.158 ± 0.31
2.241AsnPro: 2.241 ± 0.291
1.536AsnGln: 1.536 ± 0.293
2.573AsnArg: 2.573 ± 0.324
3.611AsnSer: 3.611 ± 0.329
2.656AsnThr: 2.656 ± 0.292
3.736AsnVal: 3.736 ± 0.387
0.664AsnTrp: 0.664 ± 0.16
1.619AsnTyr: 1.619 ± 0.277
0.0AsnXaa: 0.0 ± 0.0
Pro
1.785ProAla: 1.785 ± 0.315
0.872ProCys: 0.872 ± 0.236
2.49ProAsp: 2.49 ± 0.358
3.694ProGlu: 3.694 ± 0.394
1.328ProPhe: 1.328 ± 0.205
0.83ProGly: 0.83 ± 0.221
0.664ProHis: 0.664 ± 0.146
1.743ProIle: 1.743 ± 0.3
2.532ProLys: 2.532 ± 0.346
1.868ProLeu: 1.868 ± 0.284
0.623ProMet: 0.623 ± 0.156
2.034ProAsn: 2.034 ± 0.279
0.955ProPro: 0.955 ± 0.216
1.494ProGln: 1.494 ± 0.248
1.079ProArg: 1.079 ± 0.227
2.905ProSer: 2.905 ± 0.425
2.366ProThr: 2.366 ± 0.344
2.366ProVal: 2.366 ± 0.469
0.498ProTrp: 0.498 ± 0.142
1.702ProTyr: 1.702 ± 0.252
0.0ProXaa: 0.0 ± 0.0
Gln
2.905GlnAla: 2.905 ± 0.658
0.374GlnCys: 0.374 ± 0.142
1.826GlnAsp: 1.826 ± 0.23
3.071GlnGlu: 3.071 ± 0.361
1.204GlnPhe: 1.204 ± 0.222
2.283GlnGly: 2.283 ± 0.24
0.623GlnHis: 0.623 ± 0.13
2.49GlnIle: 2.49 ± 0.397
1.619GlnLys: 1.619 ± 0.248
3.653GlnLeu: 3.653 ± 0.589
1.121GlnMet: 1.121 ± 0.343
1.411GlnAsn: 1.411 ± 0.355
0.83GlnPro: 0.83 ± 0.161
1.785GlnGln: 1.785 ± 0.279
2.2GlnArg: 2.2 ± 0.451
2.781GlnSer: 2.781 ± 0.43
1.743GlnThr: 1.743 ± 0.266
2.283GlnVal: 2.283 ± 0.29
0.332GlnTrp: 0.332 ± 0.134
1.411GlnTyr: 1.411 ± 0.237
0.0GlnXaa: 0.0 ± 0.0
Arg
2.988ArgAla: 2.988 ± 0.475
0.623ArgCys: 0.623 ± 0.209
3.113ArgAsp: 3.113 ± 0.3
3.57ArgGlu: 3.57 ± 0.446
1.951ArgPhe: 1.951 ± 0.284
3.943ArgGly: 3.943 ± 0.36
0.83ArgHis: 0.83 ± 0.239
2.947ArgIle: 2.947 ± 0.36
2.905ArgLys: 2.905 ± 0.402
4.234ArgLeu: 4.234 ± 0.353
1.453ArgMet: 1.453 ± 0.236
2.573ArgAsn: 2.573 ± 0.37
1.121ArgPro: 1.121 ± 0.219
1.868ArgGln: 1.868 ± 0.353
2.698ArgArg: 2.698 ± 0.352
3.03ArgSer: 3.03 ± 0.409
2.739ArgThr: 2.739 ± 0.304
3.611ArgVal: 3.611 ± 0.375
0.955ArgTrp: 0.955 ± 0.191
1.992ArgTyr: 1.992 ± 0.273
0.0ArgXaa: 0.0 ± 0.0
Ser
3.902SerAla: 3.902 ± 0.329
0.623SerCys: 0.623 ± 0.201
4.151SerAsp: 4.151 ± 0.404
4.068SerGlu: 4.068 ± 0.313
2.615SerPhe: 2.615 ± 0.317
5.105SerGly: 5.105 ± 0.56
1.245SerHis: 1.245 ± 0.225
4.607SerIle: 4.607 ± 0.491
3.902SerLys: 3.902 ± 0.444
6.309SerLeu: 6.309 ± 0.477
1.66SerMet: 1.66 ± 0.27
2.905SerAsn: 2.905 ± 0.302
2.158SerPro: 2.158 ± 0.337
2.49SerGln: 2.49 ± 0.357
2.656SerArg: 2.656 ± 0.301
4.732SerSer: 4.732 ± 0.442
3.486SerThr: 3.486 ± 0.45
4.524SerVal: 4.524 ± 0.425
0.913SerTrp: 0.913 ± 0.222
2.49SerTyr: 2.49 ± 0.283
0.0SerXaa: 0.0 ± 0.0
Thr
2.698ThrAla: 2.698 ± 0.445
0.623ThrCys: 0.623 ± 0.163
3.071ThrAsp: 3.071 ± 0.304
4.192ThrGlu: 4.192 ± 0.435
2.283ThrPhe: 2.283 ± 0.303
4.815ThrGly: 4.815 ± 0.58
0.747ThrHis: 0.747 ± 0.154
3.653ThrIle: 3.653 ± 0.446
3.57ThrLys: 3.57 ± 0.377
5.437ThrLeu: 5.437 ± 0.35
1.245ThrMet: 1.245 ± 0.207
2.698ThrAsn: 2.698 ± 0.362
2.988ThrPro: 2.988 ± 0.537
1.494ThrGln: 1.494 ± 0.245
3.279ThrArg: 3.279 ± 0.417
4.441ThrSer: 4.441 ± 0.521
4.026ThrThr: 4.026 ± 0.507
4.109ThrVal: 4.109 ± 0.414
0.913ThrTrp: 0.913 ± 0.171
1.868ThrTyr: 1.868 ± 0.267
0.0ThrXaa: 0.0 ± 0.0
Val
4.151ValAla: 4.151 ± 0.448
0.955ValCys: 0.955 ± 0.211
5.769ValAsp: 5.769 ± 0.527
5.769ValGlu: 5.769 ± 0.632
2.324ValPhe: 2.324 ± 0.319
4.732ValGly: 4.732 ± 0.473
1.038ValHis: 1.038 ± 0.231
4.441ValIle: 4.441 ± 0.411
4.068ValLys: 4.068 ± 0.482
5.645ValLeu: 5.645 ± 0.518
2.324ValMet: 2.324 ± 0.306
2.988ValAsn: 2.988 ± 0.411
2.034ValPro: 2.034 ± 0.446
1.909ValGln: 1.909 ± 0.287
3.819ValArg: 3.819 ± 0.418
4.192ValSer: 4.192 ± 0.459
3.777ValThr: 3.777 ± 0.469
4.607ValVal: 4.607 ± 0.534
1.121ValTrp: 1.121 ± 0.202
2.988ValTyr: 2.988 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
0.706TrpAla: 0.706 ± 0.154
0.291TrpCys: 0.291 ± 0.099
1.577TrpAsp: 1.577 ± 0.244
1.494TrpGlu: 1.494 ± 0.255
0.747TrpPhe: 0.747 ± 0.211
1.245TrpGly: 1.245 ± 0.216
0.249TrpHis: 0.249 ± 0.108
0.996TrpIle: 0.996 ± 0.198
0.996TrpLys: 0.996 ± 0.214
1.494TrpLeu: 1.494 ± 0.262
0.208TrpMet: 0.208 ± 0.098
0.996TrpAsn: 0.996 ± 0.221
0.374TrpPro: 0.374 ± 0.116
0.457TrpGln: 0.457 ± 0.143
0.83TrpArg: 0.83 ± 0.213
0.872TrpSer: 0.872 ± 0.183
0.415TrpThr: 0.415 ± 0.114
1.204TrpVal: 1.204 ± 0.227
0.166TrpTrp: 0.166 ± 0.08
0.706TrpTyr: 0.706 ± 0.167
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.241TyrAla: 2.241 ± 0.29
0.789TyrCys: 0.789 ± 0.178
3.03TyrAsp: 3.03 ± 0.316
3.196TyrGlu: 3.196 ± 0.398
1.287TyrPhe: 1.287 ± 0.192
2.615TyrGly: 2.615 ± 0.344
0.872TyrHis: 0.872 ± 0.208
2.49TyrIle: 2.49 ± 0.401
3.071TyrLys: 3.071 ± 0.332
3.777TyrLeu: 3.777 ± 0.438
1.162TyrMet: 1.162 ± 0.235
1.992TyrAsn: 1.992 ± 0.315
2.034TyrPro: 2.034 ± 0.299
1.619TyrGln: 1.619 ± 0.294
1.909TyrArg: 1.909 ± 0.26
2.864TyrSer: 2.864 ± 0.3
2.324TyrThr: 2.324 ± 0.339
2.781TyrVal: 2.781 ± 0.381
0.747TyrTrp: 0.747 ± 0.172
1.453TyrTyr: 1.453 ± 0.283
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 144 proteins (24094 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski