Massilia sp. WF1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia; unclassified Massilia

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4927 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J1DI89|A0A0J1DI89_9BURK Uncharacterized protein OS=Massilia sp. WF1 OX=1406431 GN=AB595_01900 PE=4 SV=1
MM1 pKa = 7.58CLICGWVYY9 pKa = 11.38DD10 pKa = 4.41EE11 pKa = 5.35AAGSPEE17 pKa = 4.56DD18 pKa = 4.81GIPPGTRR25 pKa = 11.84WADD28 pKa = 3.16VPMNWTCPEE37 pKa = 3.86CGARR41 pKa = 11.84KK42 pKa = 9.91DD43 pKa = 3.98DD44 pKa = 4.69FEE46 pKa = 4.27MTAII50 pKa = 4.25

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J1DD95|A0A0J1DD95_9BURK Uncharacterized protein OS=Massilia sp. WF1 OX=1406431 GN=AB595_10085 PE=4 SV=1
MM1 pKa = 7.19ATSFSARR8 pKa = 11.84RR9 pKa = 11.84TAVRR13 pKa = 11.84PSTHH17 pKa = 6.68LCNAMIGALLGAAAPLHH34 pKa = 5.53AQSLFEE40 pKa = 4.28SMRR43 pKa = 11.84AGAAEE48 pKa = 3.89QPGGYY53 pKa = 9.68VFVSEE58 pKa = 4.7RR59 pKa = 11.84VTDD62 pKa = 3.54TALIPLARR70 pKa = 11.84DD71 pKa = 2.95ARR73 pKa = 11.84AAGFTIVINGFWGDD87 pKa = 3.64LNATRR92 pKa = 11.84QRR94 pKa = 11.84VARR97 pKa = 11.84INEE100 pKa = 4.01ACCGKK105 pKa = 10.25NGARR109 pKa = 11.84WQINPVLFQRR119 pKa = 11.84YY120 pKa = 6.95QVTAVPSFVISVGPGDD136 pKa = 4.11APADD140 pKa = 3.83FSKK143 pKa = 11.17VSGEE147 pKa = 3.86MSVANALKK155 pKa = 10.5FFTQQSRR162 pKa = 11.84VPAVRR167 pKa = 11.84KK168 pKa = 8.86AAQGAYY174 pKa = 8.0TRR176 pKa = 11.84AFATQQ181 pKa = 2.87

Molecular weight: 19.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4927

0

4927

1646058

37

2042

334.1

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.769 ± 0.056

0.866 ± 0.01

5.381 ± 0.026

5.342 ± 0.034

3.578 ± 0.021

8.53 ± 0.034

2.2 ± 0.017

4.549 ± 0.022

3.437 ± 0.031

10.521 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.019

2.9 ± 0.027

5.14 ± 0.028

3.791 ± 0.019

6.842 ± 0.032

5.38 ± 0.026

4.972 ± 0.029

7.401 ± 0.028

1.348 ± 0.015

2.478 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski