Amino acid dipepetide frequency for Massilia sp. WF1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.202AlaAla: 18.202 ± 0.157
1.312AlaCys: 1.312 ± 0.031
6.442AlaAsp: 6.442 ± 0.068
6.469AlaGlu: 6.469 ± 0.08
4.174AlaPhe: 4.174 ± 0.061
11.58AlaGly: 11.58 ± 0.103
2.561AlaHis: 2.561 ± 0.041
5.692AlaIle: 5.692 ± 0.062
3.858AlaLys: 3.858 ± 0.055
13.948AlaLeu: 13.948 ± 0.123
3.531AlaMet: 3.531 ± 0.048
3.296AlaAsn: 3.296 ± 0.055
6.474AlaPro: 6.474 ± 0.085
5.093AlaGln: 5.093 ± 0.066
9.189AlaArg: 9.189 ± 0.087
6.972AlaSer: 6.972 ± 0.068
5.709AlaThr: 5.709 ± 0.056
8.638AlaVal: 8.638 ± 0.083
1.826AlaTrp: 1.826 ± 0.041
2.725AlaTyr: 2.725 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
1.229CysAla: 1.229 ± 0.031
0.114CysCys: 0.114 ± 0.009
0.47CysAsp: 0.47 ± 0.017
0.445CysGlu: 0.445 ± 0.02
0.298CysPhe: 0.298 ± 0.014
0.919CysGly: 0.919 ± 0.03
0.244CysHis: 0.244 ± 0.013
0.382CysIle: 0.382 ± 0.016
0.225CysLys: 0.225 ± 0.013
0.778CysLeu: 0.778 ± 0.019
0.19CysMet: 0.19 ± 0.011
0.238CysAsn: 0.238 ± 0.013
0.411CysPro: 0.411 ± 0.017
0.227CysGln: 0.227 ± 0.012
0.566CysArg: 0.566 ± 0.019
0.508CysSer: 0.508 ± 0.019
0.433CysThr: 0.433 ± 0.018
0.633CysVal: 0.633 ± 0.02
0.125CysTrp: 0.125 ± 0.008
0.227CysTyr: 0.227 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.955AspAla: 6.955 ± 0.063
0.451AspCys: 0.451 ± 0.016
2.739AspAsp: 2.739 ± 0.049
3.27AspGlu: 3.27 ± 0.053
2.053AspPhe: 2.053 ± 0.035
4.932AspGly: 4.932 ± 0.079
1.037AspHis: 1.037 ± 0.027
2.656AspIle: 2.656 ± 0.043
1.965AspLys: 1.965 ± 0.038
5.299AspLeu: 5.299 ± 0.059
1.292AspMet: 1.292 ± 0.028
1.406AspAsn: 1.406 ± 0.032
3.23AspPro: 3.23 ± 0.047
1.739AspGln: 1.739 ± 0.033
3.304AspArg: 3.304 ± 0.043
2.398AspSer: 2.398 ± 0.041
2.682AspThr: 2.682 ± 0.049
3.818AspVal: 3.818 ± 0.052
0.933AspTrp: 0.933 ± 0.028
1.656AspTyr: 1.656 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
6.664GluAla: 6.664 ± 0.081
0.366GluCys: 0.366 ± 0.013
2.349GluAsp: 2.349 ± 0.036
2.937GluGlu: 2.937 ± 0.047
1.796GluPhe: 1.796 ± 0.031
3.702GluGly: 3.702 ± 0.051
1.394GluHis: 1.394 ± 0.032
2.714GluIle: 2.714 ± 0.046
1.991GluLys: 1.991 ± 0.041
6.159GluLeu: 6.159 ± 0.071
1.38GluMet: 1.38 ± 0.03
1.504GluAsn: 1.504 ± 0.03
2.28GluPro: 2.28 ± 0.04
2.817GluGln: 2.817 ± 0.047
4.718GluArg: 4.718 ± 0.064
2.398GluSer: 2.398 ± 0.039
2.568GluThr: 2.568 ± 0.046
3.781GluVal: 3.781 ± 0.048
0.716GluTrp: 0.716 ± 0.022
1.186GluTyr: 1.186 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.279PheAla: 4.279 ± 0.055
0.371PheCys: 0.371 ± 0.017
2.505PheAsp: 2.505 ± 0.038
1.99PheGlu: 1.99 ± 0.029
1.358PhePhe: 1.358 ± 0.034
3.621PheGly: 3.621 ± 0.063
0.758PheHis: 0.758 ± 0.023
1.579PheIle: 1.579 ± 0.034
1.177PheLys: 1.177 ± 0.028
3.078PheLeu: 3.078 ± 0.046
0.863PheMet: 0.863 ± 0.024
1.165PheAsn: 1.165 ± 0.029
1.492PhePro: 1.492 ± 0.029
1.061PheGln: 1.061 ± 0.023
2.012PheArg: 2.012 ± 0.036
2.284PheSer: 2.284 ± 0.039
2.019PheThr: 2.019 ± 0.031
2.714PheVal: 2.714 ± 0.047
0.488PheTrp: 0.488 ± 0.017
0.962PheTyr: 0.962 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
10.172GlyAla: 10.172 ± 0.091
0.833GlyCys: 0.833 ± 0.027
4.161GlyAsp: 4.161 ± 0.056
4.378GlyGlu: 4.378 ± 0.057
3.49GlyPhe: 3.49 ± 0.052
7.274GlyGly: 7.274 ± 0.104
1.899GlyHis: 1.899 ± 0.035
4.362GlyIle: 4.362 ± 0.058
3.739GlyLys: 3.739 ± 0.057
8.564GlyLeu: 8.564 ± 0.081
2.555GlyMet: 2.555 ± 0.045
2.498GlyAsn: 2.498 ± 0.056
3.152GlyPro: 3.152 ± 0.045
3.271GlyGln: 3.271 ± 0.049
5.624GlyArg: 5.624 ± 0.056
4.803GlySer: 4.803 ± 0.063
4.247GlyThr: 4.247 ± 0.061
6.375GlyVal: 6.375 ± 0.07
1.433GlyTrp: 1.433 ± 0.032
2.473GlyTyr: 2.473 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.77HisAla: 2.77 ± 0.038
0.25HisCys: 0.25 ± 0.012
1.197HisAsp: 1.197 ± 0.029
1.218HisGlu: 1.218 ± 0.029
0.888HisPhe: 0.888 ± 0.025
2.233HisGly: 2.233 ± 0.04
0.587HisHis: 0.587 ± 0.023
0.958HisIle: 0.958 ± 0.026
0.631HisLys: 0.631 ± 0.021
2.117HisLeu: 2.117 ± 0.036
0.544HisMet: 0.544 ± 0.019
0.528HisAsn: 0.528 ± 0.019
1.46HisPro: 1.46 ± 0.031
0.725HisGln: 0.725 ± 0.022
1.344HisArg: 1.344 ± 0.031
0.979HisSer: 0.979 ± 0.023
1.012HisThr: 1.012 ± 0.027
1.522HisVal: 1.522 ± 0.037
0.352HisTrp: 0.352 ± 0.015
0.691HisTyr: 0.691 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
6.352IleAla: 6.352 ± 0.065
0.374IleCys: 0.374 ± 0.014
3.293IleAsp: 3.293 ± 0.049
3.069IleGlu: 3.069 ± 0.041
1.256IlePhe: 1.256 ± 0.033
4.597IleGly: 4.597 ± 0.05
0.894IleHis: 0.894 ± 0.023
1.637IleIle: 1.637 ± 0.035
1.592IleLys: 1.592 ± 0.035
3.81IleLeu: 3.81 ± 0.046
0.912IleMet: 0.912 ± 0.023
1.47IleAsn: 1.47 ± 0.032
2.213IlePro: 2.213 ± 0.036
1.213IleGln: 1.213 ± 0.028
2.719IleArg: 2.719 ± 0.035
2.384IleSer: 2.384 ± 0.044
2.344IleThr: 2.344 ± 0.044
3.922IleVal: 3.922 ± 0.057
0.444IleTrp: 0.444 ± 0.019
0.994IleTyr: 0.994 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
4.089LysAla: 4.089 ± 0.067
0.163LysCys: 0.163 ± 0.01
1.851LysAsp: 1.851 ± 0.038
1.83LysGlu: 1.83 ± 0.039
1.001LysPhe: 1.001 ± 0.026
2.572LysGly: 2.572 ± 0.041
0.726LysHis: 0.726 ± 0.023
1.617LysIle: 1.617 ± 0.032
1.429LysLys: 1.429 ± 0.032
3.859LysLeu: 3.859 ± 0.05
0.968LysMet: 0.968 ± 0.026
1.156LysAsn: 1.156 ± 0.027
2.129LysPro: 2.129 ± 0.043
1.391LysGln: 1.391 ± 0.034
2.295LysArg: 2.295 ± 0.036
1.745LysSer: 1.745 ± 0.033
1.903LysThr: 1.903 ± 0.041
2.507LysVal: 2.507 ± 0.039
0.362LysTrp: 0.362 ± 0.015
0.78LysTyr: 0.78 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
14.882LeuAla: 14.882 ± 0.14
0.954LeuCys: 0.954 ± 0.027
6.159LeuAsp: 6.159 ± 0.066
5.44LeuGlu: 5.44 ± 0.064
3.587LeuPhe: 3.587 ± 0.053
8.619LeuGly: 8.619 ± 0.074
2.244LeuHis: 2.244 ± 0.035
4.072LeuIle: 4.072 ± 0.051
3.555LeuLys: 3.555 ± 0.05
11.082LeuLeu: 11.082 ± 0.115
2.459LeuMet: 2.459 ± 0.034
2.829LeuAsn: 2.829 ± 0.045
5.864LeuPro: 5.864 ± 0.069
3.787LeuGln: 3.787 ± 0.055
7.508LeuArg: 7.508 ± 0.082
6.092LeuSer: 6.092 ± 0.06
4.917LeuThr: 4.917 ± 0.054
7.792LeuVal: 7.792 ± 0.087
1.141LeuTrp: 1.141 ± 0.029
2.223LeuTyr: 2.223 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
3.112MetAla: 3.112 ± 0.038
0.176MetCys: 0.176 ± 0.009
1.212MetAsp: 1.212 ± 0.029
1.165MetGlu: 1.165 ± 0.029
0.814MetPhe: 0.814 ± 0.023
1.772MetGly: 1.772 ± 0.04
0.604MetHis: 0.604 ± 0.017
1.125MetIle: 1.125 ± 0.025
1.125MetLys: 1.125 ± 0.025
2.916MetLeu: 2.916 ± 0.045
0.702MetMet: 0.702 ± 0.023
0.968MetAsn: 0.968 ± 0.023
1.567MetPro: 1.567 ± 0.032
1.073MetGln: 1.073 ± 0.026
1.848MetArg: 1.848 ± 0.036
1.659MetSer: 1.659 ± 0.035
1.487MetThr: 1.487 ± 0.032
1.726MetVal: 1.726 ± 0.031
0.208MetTrp: 0.208 ± 0.011
0.489MetTyr: 0.489 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.491AsnAla: 3.491 ± 0.047
0.275AsnCys: 0.275 ± 0.013
1.494AsnAsp: 1.494 ± 0.04
1.348AsnGlu: 1.348 ± 0.028
1.092AsnPhe: 1.092 ± 0.024
2.728AsnGly: 2.728 ± 0.052
0.573AsnHis: 0.573 ± 0.019
1.418AsnIle: 1.418 ± 0.031
0.969AsnLys: 0.969 ± 0.027
2.862AsnLeu: 2.862 ± 0.043
0.688AsnMet: 0.688 ± 0.022
0.993AsnAsn: 0.993 ± 0.032
1.936AsnPro: 1.936 ± 0.039
0.987AsnGln: 0.987 ± 0.032
1.768AsnArg: 1.768 ± 0.031
1.351AsnSer: 1.351 ± 0.032
1.563AsnThr: 1.563 ± 0.035
2.164AsnVal: 2.164 ± 0.044
0.447AsnTrp: 0.447 ± 0.016
0.853AsnTyr: 0.853 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
7.223ProAla: 7.223 ± 0.092
0.326ProCys: 0.326 ± 0.014
3.293ProAsp: 3.293 ± 0.044
3.287ProGlu: 3.287 ± 0.051
1.837ProPhe: 1.837 ± 0.033
4.808ProGly: 4.808 ± 0.063
1.064ProHis: 1.064 ± 0.028
1.929ProIle: 1.929 ± 0.037
1.593ProLys: 1.593 ± 0.034
5.154ProLeu: 5.154 ± 0.058
1.22ProMet: 1.22 ± 0.029
1.452ProAsn: 1.452 ± 0.032
2.423ProPro: 2.423 ± 0.05
2.049ProGln: 2.049 ± 0.039
2.775ProArg: 2.775 ± 0.047
2.691ProSer: 2.691 ± 0.039
2.262ProThr: 2.262 ± 0.039
4.169ProVal: 4.169 ± 0.053
0.662ProTrp: 0.662 ± 0.023
1.184ProTyr: 1.184 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
5.42GlnAla: 5.42 ± 0.07
0.266GlnCys: 0.266 ± 0.012
1.775GlnAsp: 1.775 ± 0.031
1.758GlnGlu: 1.758 ± 0.034
1.244GlnPhe: 1.244 ± 0.027
2.735GlnGly: 2.735 ± 0.045
0.891GlnHis: 0.891 ± 0.024
1.597GlnIle: 1.597 ± 0.03
1.228GlnLys: 1.228 ± 0.029
4.053GlnLeu: 4.053 ± 0.052
1.035GlnMet: 1.035 ± 0.024
0.996GlnAsn: 0.996 ± 0.029
2.074GlnPro: 2.074 ± 0.04
1.977GlnGln: 1.977 ± 0.043
2.928GlnArg: 2.928 ± 0.044
1.882GlnSer: 1.882 ± 0.034
1.714GlnThr: 1.714 ± 0.036
2.996GlnVal: 2.996 ± 0.043
0.449GlnTrp: 0.449 ± 0.016
0.892GlnTyr: 0.892 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
7.818ArgAla: 7.818 ± 0.083
0.525ArgCys: 0.525 ± 0.02
3.638ArgAsp: 3.638 ± 0.042
4.145ArgGlu: 4.145 ± 0.05
2.809ArgPhe: 2.809 ± 0.036
4.479ArgGly: 4.479 ± 0.054
1.811ArgHis: 1.811 ± 0.033
3.774ArgIle: 3.774 ± 0.046
2.312ArgLys: 2.312 ± 0.037
7.625ArgLeu: 7.625 ± 0.079
1.921ArgMet: 1.921 ± 0.031
1.965ArgAsn: 1.965 ± 0.039
3.183ArgPro: 3.183 ± 0.049
2.953ArgGln: 2.953 ± 0.044
5.152ArgArg: 5.152 ± 0.066
3.418ArgSer: 3.418 ± 0.043
3.19ArgThr: 3.19 ± 0.046
4.711ArgVal: 4.711 ± 0.05
0.97ArgTrp: 0.97 ± 0.031
2.02ArgTyr: 2.02 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
6.383SerAla: 6.383 ± 0.07
0.441SerCys: 0.441 ± 0.017
2.59SerAsp: 2.59 ± 0.045
2.457SerGlu: 2.457 ± 0.037
2.188SerPhe: 2.188 ± 0.039
5.291SerGly: 5.291 ± 0.067
1.222SerHis: 1.222 ± 0.03
2.527SerIle: 2.527 ± 0.043
1.775SerLys: 1.775 ± 0.037
5.768SerLeu: 5.768 ± 0.061
1.504SerMet: 1.504 ± 0.031
1.588SerAsn: 1.588 ± 0.033
2.671SerPro: 2.671 ± 0.044
1.802SerGln: 1.802 ± 0.035
3.251SerArg: 3.251 ± 0.042
3.314SerSer: 3.314 ± 0.049
2.924SerThr: 2.924 ± 0.038
3.911SerVal: 3.911 ± 0.051
0.782SerTrp: 0.782 ± 0.025
1.411SerTyr: 1.411 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
5.696ThrAla: 5.696 ± 0.074
0.376ThrCys: 0.376 ± 0.015
2.426ThrAsp: 2.426 ± 0.044
2.214ThrGlu: 2.214 ± 0.038
1.642ThrPhe: 1.642 ± 0.035
4.786ThrGly: 4.786 ± 0.065
0.988ThrHis: 0.988 ± 0.024
2.471ThrIle: 2.471 ± 0.045
1.29ThrLys: 1.29 ± 0.03
5.683ThrLeu: 5.683 ± 0.061
1.279ThrMet: 1.279 ± 0.032
1.389ThrAsn: 1.389 ± 0.034
3.176ThrPro: 3.176 ± 0.046
1.616ThrGln: 1.616 ± 0.031
3.251ThrArg: 3.251 ± 0.043
2.631ThrSer: 2.631 ± 0.048
2.632ThrThr: 2.632 ± 0.052
4.286ThrVal: 4.286 ± 0.051
0.664ThrTrp: 0.664 ± 0.021
1.228ThrTyr: 1.228 ± 0.03
0.0ThrXaa: 0.0 ± 0.0
Val
8.926ValAla: 8.926 ± 0.073
0.691ValCys: 0.691 ± 0.023
4.19ValAsp: 4.19 ± 0.049
4.17ValGlu: 4.17 ± 0.055
2.674ValPhe: 2.674 ± 0.046
5.514ValGly: 5.514 ± 0.061
1.608ValHis: 1.608 ± 0.032
3.394ValIle: 3.394 ± 0.05
2.592ValLys: 2.592 ± 0.041
8.175ValLeu: 8.175 ± 0.081
1.8ValMet: 1.8 ± 0.034
2.343ValAsn: 2.343 ± 0.045
3.788ValPro: 3.788 ± 0.052
2.568ValGln: 2.568 ± 0.041
5.04ValArg: 5.04 ± 0.052
4.175ValSer: 4.175 ± 0.049
4.044ValThr: 4.044 ± 0.054
5.765ValVal: 5.765 ± 0.065
0.849ValTrp: 0.849 ± 0.022
1.699ValTyr: 1.699 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
1.155TrpAla: 1.155 ± 0.029
0.144TrpCys: 0.144 ± 0.009
0.662TrpAsp: 0.662 ± 0.025
0.585TrpGlu: 0.585 ± 0.02
0.573TrpPhe: 0.573 ± 0.019
0.856TrpGly: 0.856 ± 0.025
0.344TrpHis: 0.344 ± 0.015
0.693TrpIle: 0.693 ± 0.022
0.495TrpLys: 0.495 ± 0.017
1.745TrpLeu: 1.745 ± 0.04
0.416TrpMet: 0.416 ± 0.017
0.482TrpAsn: 0.482 ± 0.02
0.627TrpPro: 0.627 ± 0.019
0.622TrpGln: 0.622 ± 0.02
1.15TrpArg: 1.15 ± 0.027
0.795TrpSer: 0.795 ± 0.023
0.735TrpThr: 0.735 ± 0.023
0.825TrpVal: 0.825 ± 0.024
0.232TrpTrp: 0.232 ± 0.012
0.345TrpTyr: 0.345 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.873TyrAla: 2.873 ± 0.04
0.252TyrCys: 0.252 ± 0.011
1.37TyrAsp: 1.37 ± 0.037
1.244TyrGlu: 1.244 ± 0.026
1.002TyrPhe: 1.002 ± 0.026
2.285TyrGly: 2.285 ± 0.033
0.555TyrHis: 0.555 ± 0.019
0.893TyrIle: 0.893 ± 0.027
0.832TyrLys: 0.832 ± 0.023
2.532TyrLeu: 2.532 ± 0.04
0.491TyrMet: 0.491 ± 0.017
0.731TyrAsn: 0.731 ± 0.025
1.253TyrPro: 1.253 ± 0.031
0.936TyrGln: 0.936 ± 0.024
1.977TyrArg: 1.977 ± 0.036
1.321TyrSer: 1.321 ± 0.032
1.34TyrThr: 1.34 ± 0.036
1.75TyrVal: 1.75 ± 0.032
0.398TyrTrp: 0.398 ± 0.017
0.738TyrTyr: 0.738 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4927 proteins (1646058 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski