Sphingobium sp. Leaf26

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4J302|A0A0Q4J302_9SPHN Ubiquinone biosynthesis O-methyltransferase OS=Sphingobium sp. Leaf26 OX=1735693 GN=ubiG PE=3 SV=1
MM1 pKa = 7.69ANFSGTSGNDD11 pKa = 2.43IFTGGDD17 pKa = 3.41EE18 pKa = 5.11DD19 pKa = 5.39DD20 pKa = 4.04VATGGLGNDD29 pKa = 3.48RR30 pKa = 11.84LTGGEE35 pKa = 4.17GSDD38 pKa = 3.58ILSGDD43 pKa = 3.59EE44 pKa = 5.33GADD47 pKa = 3.46TLNGGGGDD55 pKa = 4.34DD56 pKa = 3.96YY57 pKa = 11.5LFSAGRR63 pKa = 11.84YY64 pKa = 8.43YY65 pKa = 11.05DD66 pKa = 3.88FSFSGIYY73 pKa = 9.8YY74 pKa = 10.0LNKK77 pKa = 9.68PVLDD81 pKa = 4.04TGAEE85 pKa = 4.02ADD87 pKa = 4.01TVLGGNGDD95 pKa = 3.98DD96 pKa = 5.26VIFAGYY102 pKa = 10.57NDD104 pKa = 4.69VIDD107 pKa = 4.49GGAGGEE113 pKa = 4.07DD114 pKa = 3.03TLYY117 pKa = 10.68FSLMGATHH125 pKa = 6.96GVTMDD130 pKa = 3.5FRR132 pKa = 11.84LPVIANGTGSISGIEE147 pKa = 4.04NIGWVQGSNYY157 pKa = 10.2GDD159 pKa = 3.67TLILDD164 pKa = 3.85QQGSYY169 pKa = 11.39GPFGVVYY176 pKa = 10.95AMGGDD181 pKa = 3.72DD182 pKa = 4.65HH183 pKa = 8.17VVASYY188 pKa = 8.97YY189 pKa = 9.91TDD191 pKa = 4.12HH192 pKa = 7.33IYY194 pKa = 11.08GGGGNDD200 pKa = 3.44ILDD203 pKa = 4.24GRR205 pKa = 11.84PSGYY209 pKa = 10.65LDD211 pKa = 3.69ILDD214 pKa = 4.85GGDD217 pKa = 4.15GNDD220 pKa = 3.38TLYY223 pKa = 11.47SNDD226 pKa = 3.5GSGEE230 pKa = 4.16AYY232 pKa = 10.19GGNGDD237 pKa = 3.64DD238 pKa = 4.02TIYY241 pKa = 11.06AGGLVRR247 pKa = 11.84GGAGNDD253 pKa = 3.64RR254 pKa = 11.84IVMQSSYY261 pKa = 9.34YY262 pKa = 9.71HH263 pKa = 6.24SGVFGDD269 pKa = 4.16EE270 pKa = 4.26GNDD273 pKa = 3.53EE274 pKa = 4.06IRR276 pKa = 11.84TSRR279 pKa = 11.84EE280 pKa = 3.72GNVVSGGSGADD291 pKa = 3.54RR292 pKa = 11.84LIGDD296 pKa = 4.78AGSDD300 pKa = 3.36TLIAGDD306 pKa = 4.29YY307 pKa = 10.89LPGPYY312 pKa = 9.78YY313 pKa = 10.77VGSHH317 pKa = 6.97DD318 pKa = 4.04MGTEE322 pKa = 3.83RR323 pKa = 11.84DD324 pKa = 3.93VLSGDD329 pKa = 3.66GGDD332 pKa = 4.6DD333 pKa = 3.51ILWAGWGDD341 pKa = 4.04SVDD344 pKa = 3.96GGSGTDD350 pKa = 2.82RR351 pKa = 11.84LYY353 pKa = 11.33YY354 pKa = 10.57SLGGATAGVALSTGIFASASPQALGGGTVQNVEE387 pKa = 4.2RR388 pKa = 11.84LVEE391 pKa = 4.03LMGSAFADD399 pKa = 4.92RR400 pKa = 11.84ITVATQDD407 pKa = 3.39SLLTIHH413 pKa = 7.18AGAGDD418 pKa = 4.07DD419 pKa = 4.07VVTTGDD425 pKa = 3.96SSVTLNGGDD434 pKa = 3.64GNDD437 pKa = 3.39RR438 pKa = 11.84LVSGAAADD446 pKa = 4.09SFDD449 pKa = 3.71GGAGVDD455 pKa = 3.85TIDD458 pKa = 3.32YY459 pKa = 9.44DD460 pKa = 4.07AYY462 pKa = 10.87ASGVSVNLLVGKK474 pKa = 10.01GAGGDD479 pKa = 3.41SLRR482 pKa = 11.84NVEE485 pKa = 4.81NIVGSAFADD494 pKa = 3.66TLVGDD499 pKa = 4.59GSANLITAGAGNDD512 pKa = 3.96SLDD515 pKa = 3.75GGAGADD521 pKa = 3.57RR522 pKa = 11.84LVGGLGNDD530 pKa = 3.25TYY532 pKa = 11.79YY533 pKa = 10.99VDD535 pKa = 3.66NVGDD539 pKa = 3.89VVVEE543 pKa = 4.09QAGEE547 pKa = 4.37GYY549 pKa = 10.73DD550 pKa = 3.88VVITNIDD557 pKa = 3.46YY558 pKa = 9.28TLGANVEE565 pKa = 4.53GIKK568 pKa = 10.57LLNLTKK574 pKa = 10.46GVEE577 pKa = 3.93VHH579 pKa = 7.0GNGLNNSVNAAYY591 pKa = 10.29ASAPLAAGTQIRR603 pKa = 11.84MFGEE607 pKa = 4.76GGDD610 pKa = 3.84DD611 pKa = 3.31TLLGSRR617 pKa = 11.84HH618 pKa = 6.96DD619 pKa = 4.74DD620 pKa = 3.63YY621 pKa = 11.91LDD623 pKa = 3.81GGTGVDD629 pKa = 3.68TMTGGLGNDD638 pKa = 3.57SYY640 pKa = 12.02VVDD643 pKa = 4.22DD644 pKa = 4.38ARR646 pKa = 11.84DD647 pKa = 3.7VVVEE651 pKa = 3.99QAGQGYY657 pKa = 9.9DD658 pKa = 3.5VVITSIDD665 pKa = 3.53YY666 pKa = 8.84TLGANVEE673 pKa = 4.54GIKK676 pKa = 10.47LLNVTRR682 pKa = 11.84GVEE685 pKa = 3.8VHH687 pKa = 6.67GNSLNNSFNAAYY699 pKa = 10.33VGASLAAGTQIRR711 pKa = 11.84MFGEE715 pKa = 4.63GGNDD719 pKa = 3.09TLLGSRR725 pKa = 11.84HH726 pKa = 6.08GDD728 pKa = 3.62YY729 pKa = 11.15LDD731 pKa = 4.14GGAGIDD737 pKa = 3.56TMTGGFGNDD746 pKa = 3.08TYY748 pKa = 11.83VVDD751 pKa = 3.81NKK753 pKa = 11.02GDD755 pKa = 3.76VVVEE759 pKa = 4.04QAGQGYY765 pKa = 10.09DD766 pKa = 3.49VVITNIDD773 pKa = 3.46YY774 pKa = 9.28TLGANVEE781 pKa = 4.53GIKK784 pKa = 10.54LLNLTRR790 pKa = 11.84GVEE793 pKa = 3.91VHH795 pKa = 6.92GNGLNNSLNAAYY807 pKa = 10.48ANGPLAAGTKK817 pKa = 9.24IQMFGEE823 pKa = 4.77GGNDD827 pKa = 3.14TLLGSRR833 pKa = 11.84NDD835 pKa = 4.08DD836 pKa = 3.75YY837 pKa = 11.92LDD839 pKa = 3.95GGTGVDD845 pKa = 3.42TMTGGFGNDD854 pKa = 3.02TYY856 pKa = 11.78VVDD859 pKa = 3.77NAGDD863 pKa = 3.5IVVEE867 pKa = 4.15QGGQGYY873 pKa = 9.88DD874 pKa = 3.33VVVTSIDD881 pKa = 3.6YY882 pKa = 9.39MLGANVEE889 pKa = 4.22GLKK892 pKa = 10.68LLNLTKK898 pKa = 10.55GVEE901 pKa = 3.87AHH903 pKa = 7.05GNGLNNSLNAAYY915 pKa = 10.91VNTPLAAGTTIRR927 pKa = 11.84LFGEE931 pKa = 4.68GGSDD935 pKa = 3.21SLLGSRR941 pKa = 11.84YY942 pKa = 10.36GDD944 pKa = 3.75FLDD947 pKa = 5.22GGAGNDD953 pKa = 3.67TLQGGGGNDD962 pKa = 2.98RR963 pKa = 11.84FHH965 pKa = 7.65FGDD968 pKa = 4.07GLHH971 pKa = 6.73ALSNVDD977 pKa = 4.04TILDD981 pKa = 3.79FQLGDD986 pKa = 3.86MIEE989 pKa = 4.7LDD991 pKa = 3.4DD992 pKa = 4.88AVFNGLSTGALGAGAFVANAAGAATSAEE1020 pKa = 3.73QRR1022 pKa = 11.84IVYY1025 pKa = 8.28DD1026 pKa = 3.62TDD1028 pKa = 3.28SGQLFFDD1035 pKa = 4.6ADD1037 pKa = 3.55GSGAGGAVLFAVLNGHH1053 pKa = 6.23PAIGAADD1060 pKa = 3.72FVVVV1064 pKa = 4.09

Molecular weight: 107.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4III5|A0A0Q4III5_9SPHN Uncharacterized protein OS=Sphingobium sp. Leaf26 OX=1735693 GN=ASE85_08780 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.06SLSAA44 pKa = 3.93

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4284

0

4284

1403714

29

3092

327.7

35.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.055 ± 0.059

0.767 ± 0.01

6.264 ± 0.031

4.868 ± 0.039

3.527 ± 0.023

8.941 ± 0.042

2.05 ± 0.019

5.204 ± 0.023

3.048 ± 0.035

9.877 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.038

2.615 ± 0.028

5.307 ± 0.032

3.337 ± 0.024

7.11 ± 0.041

5.289 ± 0.036

5.298 ± 0.04

6.976 ± 0.029

1.476 ± 0.017

2.349 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski