Amino acid dipepetide frequency for Sphingobium sp. Leaf26

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.858AlaAla: 17.858 ± 0.182	1.053AlaCys: 1.053 ± 0.026	8.156AlaAsp: 8.156 ± 0.081	6.377AlaGlu: 6.377 ± 0.075	4.304AlaPhe: 4.304 ± 0.059	11.077AlaGly: 11.077 ± 0.108	2.427AlaHis: 2.427 ± 0.044	7.128AlaIle: 7.128 ± 0.077	4.059AlaLys: 4.059 ± 0.063	13.993AlaLeu: 13.993 ± 0.134	4.135AlaMet: 4.135 ± 0.07	3.243AlaAsn: 3.243 ± 0.058	6.41AlaPro: 6.41 ± 0.095	5.136AlaGln: 5.136 ± 0.078	9.222AlaArg: 9.222 ± 0.102	6.596AlaSer: 6.596 ± 0.091	6.707AlaThr: 6.707 ± 0.092	8.278AlaVal: 8.278 ± 0.085	1.606AlaTrp: 1.606 ± 0.039	2.787AlaTyr: 2.787 ± 0.051	0.001AlaXaa: 0.001 ± 0.001
Cys	1.003CysAla: 1.003 ± 0.025	0.093CysCys: 0.093 ± 0.008	0.501CysAsp: 0.501 ± 0.022	0.362CysGlu: 0.362 ± 0.018	0.274CysPhe: 0.274 ± 0.015	0.843CysGly: 0.843 ± 0.026	0.208CysHis: 0.208 ± 0.011	0.354CysIle: 0.354 ± 0.018	0.158CysLys: 0.158 ± 0.01	0.702CysLeu: 0.702 ± 0.021	0.162CysMet: 0.162 ± 0.011	0.199CysAsn: 0.199 ± 0.011	0.423CysPro: 0.423 ± 0.018	0.212CysGln: 0.212 ± 0.011	0.518CysArg: 0.518 ± 0.019	0.405CysSer: 0.405 ± 0.015	0.404CysThr: 0.404 ± 0.015	0.529CysVal: 0.529 ± 0.02	0.137CysTrp: 0.137 ± 0.011	0.182CysTyr: 0.182 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	8.085AspAla: 8.085 ± 0.083	0.472AspCys: 0.472 ± 0.017	3.485AspAsp: 3.485 ± 0.049	3.109AspGlu: 3.109 ± 0.057	2.212AspPhe: 2.212 ± 0.04	6.199AspGly: 6.199 ± 0.089	1.517AspHis: 1.517 ± 0.033	3.345AspIle: 3.345 ± 0.054	1.918AspLys: 1.918 ± 0.042	5.899AspLeu: 5.899 ± 0.069	1.748AspMet: 1.748 ± 0.039	1.516AspAsn: 1.516 ± 0.03	3.69AspPro: 3.69 ± 0.054	1.995AspGln: 1.995 ± 0.039	5.062AspArg: 5.062 ± 0.067	2.496AspSer: 2.496 ± 0.05	2.627AspThr: 2.627 ± 0.05	4.205AspVal: 4.205 ± 0.056	1.238AspTrp: 1.238 ± 0.03	1.822AspTyr: 1.822 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	6.979GluAla: 6.979 ± 0.076	0.264GluCys: 0.264 ± 0.015	2.622GluAsp: 2.622 ± 0.044	2.674GluGlu: 2.674 ± 0.059	1.308GluPhe: 1.308 ± 0.031	4.255GluGly: 4.255 ± 0.067	0.945GluHis: 0.945 ± 0.027	2.605GluIle: 2.605 ± 0.05	1.934GluLys: 1.934 ± 0.04	4.237GluLeu: 4.237 ± 0.057	1.326GluMet: 1.326 ± 0.036	1.24GluAsn: 1.24 ± 0.031	2.239GluPro: 2.239 ± 0.04	2.106GluGln: 2.106 ± 0.042	4.279GluArg: 4.279 ± 0.063	2.134GluSer: 2.134 ± 0.04	2.86GluThr: 2.86 ± 0.05	3.075GluVal: 3.075 ± 0.05	0.671GluTrp: 0.671 ± 0.021	0.928GluTyr: 0.928 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.705PheAla: 4.705 ± 0.067	0.316PheCys: 0.316 ± 0.014	2.737PheAsp: 2.737 ± 0.04	1.784PheGlu: 1.784 ± 0.039	1.272PhePhe: 1.272 ± 0.037	3.623PheGly: 3.623 ± 0.066	0.753PheHis: 0.753 ± 0.025	1.522PheIle: 1.522 ± 0.038	0.873PheLys: 0.873 ± 0.027	3.074PheLeu: 3.074 ± 0.053	0.826PheMet: 0.826 ± 0.025	1.069PheAsn: 1.069 ± 0.032	1.567PhePro: 1.567 ± 0.033	0.898PheGln: 0.898 ± 0.029	2.217PheArg: 2.217 ± 0.039	2.12PheSer: 2.12 ± 0.043	2.102PheThr: 2.102 ± 0.042	2.413PheVal: 2.413 ± 0.047	0.497PheTrp: 0.497 ± 0.018	0.904PheTyr: 0.904 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	10.033GlyAla: 10.033 ± 0.113	0.783GlyCys: 0.783 ± 0.023	5.159GlyAsp: 5.159 ± 0.079	4.475GlyGlu: 4.475 ± 0.059	3.713GlyPhe: 3.713 ± 0.054	8.121GlyGly: 8.121 ± 0.139	1.934GlyHis: 1.934 ± 0.041	4.382GlyIle: 4.382 ± 0.063	3.394GlyLys: 3.394 ± 0.06	8.866GlyLeu: 8.866 ± 0.088	2.415GlyMet: 2.415 ± 0.044	2.478GlyAsn: 2.478 ± 0.072	3.603GlyPro: 3.603 ± 0.054	3.348GlyGln: 3.348 ± 0.048	6.358GlyArg: 6.358 ± 0.067	4.923GlySer: 4.923 ± 0.068	4.928GlyThr: 4.928 ± 0.106	6.238GlyVal: 6.238 ± 0.07	1.725GlyTrp: 1.725 ± 0.036	2.528GlyTyr: 2.528 ± 0.047	0.001GlyXaa: 0.001 ± 0.001
His	2.433HisAla: 2.433 ± 0.047	0.207HisCys: 0.207 ± 0.012	1.353HisAsp: 1.353 ± 0.032	0.919HisGlu: 0.919 ± 0.023	0.88HisPhe: 0.88 ± 0.027	2.112HisGly: 2.112 ± 0.041	0.596HisHis: 0.596 ± 0.021	1.037HisIle: 1.037 ± 0.029	0.477HisLys: 0.477 ± 0.019	1.941HisLeu: 1.941 ± 0.042	0.581HisMet: 0.581 ± 0.02	0.453HisAsn: 0.453 ± 0.019	1.253HisPro: 1.253 ± 0.031	0.598HisGln: 0.598 ± 0.019	1.46HisArg: 1.46 ± 0.032	0.93HisSer: 0.93 ± 0.025	0.564HisThr: 0.564 ± 0.022	1.658HisVal: 1.658 ± 0.037	0.41HisTrp: 0.41 ± 0.02	0.64HisTyr: 0.64 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	8.064IleAla: 8.064 ± 0.089	0.442IleCys: 0.442 ± 0.019	4.049IleAsp: 4.049 ± 0.051	3.157IleGlu: 3.157 ± 0.049	1.736IlePhe: 1.736 ± 0.043	5.364IleGly: 5.364 ± 0.068	0.97IleHis: 0.97 ± 0.025	2.336IleIle: 2.336 ± 0.044	1.265IleLys: 1.265 ± 0.033	4.43IleLeu: 4.43 ± 0.063	1.012IleMet: 1.012 ± 0.032	1.396IleAsn: 1.396 ± 0.035	2.249IlePro: 2.249 ± 0.032	1.243IleGln: 1.243 ± 0.03	3.251IleArg: 3.251 ± 0.048	2.716IleSer: 2.716 ± 0.047	2.456IleThr: 2.456 ± 0.046	4.194IleVal: 4.194 ± 0.058	0.665IleTrp: 0.665 ± 0.024	1.04IleTyr: 1.04 ± 0.03	0.001IleXaa: 0.001 ± 0.001
Lys	4.423LysAla: 4.423 ± 0.069	0.16LysCys: 0.16 ± 0.011	1.71LysAsp: 1.71 ± 0.039	1.226LysGlu: 1.226 ± 0.037	0.811LysPhe: 0.811 ± 0.023	2.903LysGly: 2.903 ± 0.054	0.47LysHis: 0.47 ± 0.018	1.553LysIle: 1.553 ± 0.032	1.094LysLys: 1.094 ± 0.036	3.107LysLeu: 3.107 ± 0.051	0.765LysMet: 0.765 ± 0.025	0.751LysAsn: 0.751 ± 0.024	2.025LysPro: 2.025 ± 0.046	0.912LysGln: 0.912 ± 0.028	2.146LysArg: 2.146 ± 0.039	1.575LysSer: 1.575 ± 0.038	1.634LysThr: 1.634 ± 0.04	2.191LysVal: 2.191 ± 0.042	0.411LysTrp: 0.411 ± 0.017	0.612LysTyr: 0.612 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.184LeuAla: 13.184 ± 0.125	0.863LeuCys: 0.863 ± 0.024	6.114LeuAsp: 6.114 ± 0.075	4.375LeuGlu: 4.375 ± 0.069	3.798LeuPhe: 3.798 ± 0.06	8.102LeuGly: 8.102 ± 0.076	1.875LeuHis: 1.875 ± 0.041	5.107LeuIle: 5.107 ± 0.068	2.997LeuLys: 2.997 ± 0.047	9.991LeuLeu: 9.991 ± 0.131	2.49LeuMet: 2.49 ± 0.115	2.52LeuAsn: 2.52 ± 0.046	5.785LeuPro: 5.785 ± 0.076	2.433LeuGln: 2.433 ± 0.056	6.598LeuArg: 6.598 ± 0.077	6.357LeuSer: 6.357 ± 0.071	5.934LeuThr: 5.934 ± 0.07	6.787LeuVal: 6.787 ± 0.08	1.343LeuTrp: 1.343 ± 0.032	2.117LeuTyr: 2.117 ± 0.038	0.0LeuXaa: 0.0 ± 0.0
Met	3.614MetAla: 3.614 ± 0.059	0.133MetCys: 0.133 ± 0.009	1.363MetAsp: 1.363 ± 0.035	1.098MetGlu: 1.098 ± 0.032	0.696MetPhe: 0.696 ± 0.024	2.119MetGly: 2.119 ± 0.04	0.403MetHis: 0.403 ± 0.017	1.401MetIle: 1.401 ± 0.033	0.967MetLys: 0.967 ± 0.026	2.954MetLeu: 2.954 ± 0.138	0.745MetMet: 0.745 ± 0.025	0.695MetAsn: 0.695 ± 0.021	1.648MetPro: 1.648 ± 0.078	1.022MetGln: 1.022 ± 0.093	1.944MetArg: 1.944 ± 0.092	1.478MetSer: 1.478 ± 0.033	1.896MetThr: 1.896 ± 0.042	1.755MetVal: 1.755 ± 0.038	0.222MetTrp: 0.222 ± 0.013	0.274MetTyr: 0.274 ± 0.014	0.003MetXaa: 0.003 ± 0.002
Asn	3.283AsnAla: 3.283 ± 0.057	0.214AsnCys: 0.214 ± 0.013	1.567AsnAsp: 1.567 ± 0.039	1.048AsnGlu: 1.048 ± 0.032	0.975AsnPhe: 0.975 ± 0.031	2.674AsnGly: 2.674 ± 0.059	0.499AsnHis: 0.499 ± 0.018	1.465AsnIle: 1.465 ± 0.034	0.686AsnLys: 0.686 ± 0.026	2.478AsnLeu: 2.478 ± 0.051	0.606AsnMet: 0.606 ± 0.022	0.732AsnAsn: 0.732 ± 0.029	1.764AsnPro: 1.764 ± 0.041	0.755AsnGln: 0.755 ± 0.025	1.864AsnArg: 1.864 ± 0.037	1.415AsnSer: 1.415 ± 0.044	0.998AsnThr: 0.998 ± 0.038	1.911AsnVal: 1.911 ± 0.049	0.487AsnTrp: 0.487 ± 0.02	0.732AsnTyr: 0.732 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.121ProAla: 7.121 ± 0.087	0.31ProCys: 0.31 ± 0.017	4.117ProAsp: 4.117 ± 0.061	2.894ProGlu: 2.894 ± 0.051	1.98ProPhe: 1.98 ± 0.04	4.645ProGly: 4.645 ± 0.056	1.107ProHis: 1.107 ± 0.029	2.597ProIle: 2.597 ± 0.04	1.493ProLys: 1.493 ± 0.039	4.963ProLeu: 4.963 ± 0.067	1.413ProMet: 1.413 ± 0.07	1.263ProAsn: 1.263 ± 0.035	2.723ProPro: 2.723 ± 0.066	1.822ProGln: 1.822 ± 0.032	2.924ProArg: 2.924 ± 0.049	2.694ProSer: 2.694 ± 0.048	2.694ProThr: 2.694 ± 0.051	4.411ProVal: 4.411 ± 0.07	0.729ProTrp: 0.729 ± 0.024	1.166ProTyr: 1.166 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.691GlnAla: 4.691 ± 0.065	0.246GlnCys: 0.246 ± 0.012	1.752GlnAsp: 1.752 ± 0.041	1.384GlnGlu: 1.384 ± 0.029	1.1GlnPhe: 1.1 ± 0.033	2.793GlnGly: 2.793 ± 0.049	0.624GlnHis: 0.624 ± 0.024	1.852GlnIle: 1.852 ± 0.039	1.004GlnLys: 1.004 ± 0.031	2.994GlnLeu: 2.994 ± 0.053	1.099GlnMet: 1.099 ± 0.089	0.811GlnAsn: 0.811 ± 0.024	1.943GlnPro: 1.943 ± 0.042	1.295GlnGln: 1.295 ± 0.033	2.591GlnArg: 2.591 ± 0.051	1.887GlnSer: 1.887 ± 0.039	1.735GlnThr: 1.735 ± 0.032	2.356GlnVal: 2.356 ± 0.04	0.536GlnTrp: 0.536 ± 0.018	0.674GlnTyr: 0.674 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.443ArgAla: 8.443 ± 0.092	0.51ArgCys: 0.51 ± 0.021	4.376ArgAsp: 4.376 ± 0.058	3.427ArgGlu: 3.427 ± 0.058	3.012ArgPhe: 3.012 ± 0.049	4.807ArgGly: 4.807 ± 0.063	1.802ArgHis: 1.802 ± 0.036	4.197ArgIle: 4.197 ± 0.061	2.095ArgLys: 2.095 ± 0.041	7.871ArgLeu: 7.871 ± 0.093	2.086ArgMet: 2.086 ± 0.107	1.88ArgAsn: 1.88 ± 0.042	3.714ArgPro: 3.714 ± 0.056	2.655ArgGln: 2.655 ± 0.05	5.409ArgArg: 5.409 ± 0.08	3.618ArgSer: 3.618 ± 0.055	3.561ArgThr: 3.561 ± 0.055	4.39ArgVal: 4.39 ± 0.054	1.277ArgTrp: 1.277 ± 0.03	1.971ArgTyr: 1.971 ± 0.034	0.001ArgXaa: 0.001 ± 0.001
Ser	6.54SerAla: 6.54 ± 0.1	0.405SerCys: 0.405 ± 0.017	3.266SerAsp: 3.266 ± 0.051	2.263SerGlu: 2.263 ± 0.044	2.238SerPhe: 2.238 ± 0.039	5.498SerGly: 5.498 ± 0.078	1.017SerHis: 1.017 ± 0.027	2.859SerIle: 2.859 ± 0.051	1.396SerLys: 1.396 ± 0.038	5.322SerLeu: 5.322 ± 0.07	1.234SerMet: 1.234 ± 0.031	1.409SerAsn: 1.409 ± 0.039	2.897SerPro: 2.897 ± 0.047	1.601SerGln: 1.601 ± 0.034	3.529SerArg: 3.529 ± 0.053	2.736SerSer: 2.736 ± 0.063	2.627SerThr: 2.627 ± 0.059	3.751SerVal: 3.751 ± 0.054	0.807SerTrp: 0.807 ± 0.023	1.497SerTyr: 1.497 ± 0.038	0.001SerXaa: 0.001 ± 0.001
Thr	6.324ThrAla: 6.324 ± 0.084	0.373ThrCys: 0.373 ± 0.018	3.18ThrAsp: 3.18 ± 0.051	2.109ThrGlu: 2.109 ± 0.037	1.67ThrPhe: 1.67 ± 0.036	5.391ThrGly: 5.391 ± 0.074	1.025ThrHis: 1.025 ± 0.025	3.12ThrIle: 3.12 ± 0.056	1.344ThrLys: 1.344 ± 0.033	6.147ThrLeu: 6.147 ± 0.07	1.191ThrMet: 1.191 ± 0.03	1.367ThrAsn: 1.367 ± 0.044	3.528ThrPro: 3.528 ± 0.06	1.654ThrGln: 1.654 ± 0.037	3.31ThrArg: 3.31 ± 0.06	2.628ThrSer: 2.628 ± 0.057	2.679ThrThr: 2.679 ± 0.062	4.134ThrVal: 4.134 ± 0.073	0.581ThrTrp: 0.581 ± 0.022	1.216ThrTyr: 1.216 ± 0.037	0.004ThrXaa: 0.004 ± 0.002
Val	9.54ValAla: 9.54 ± 0.093	0.469ValCys: 0.469 ± 0.018	4.606ValAsp: 4.606 ± 0.066	4.267ValGlu: 4.267 ± 0.062	1.859ValPhe: 1.859 ± 0.04	5.619ValGly: 5.619 ± 0.07	1.383ValHis: 1.383 ± 0.036	3.564ValIle: 3.564 ± 0.06	2.165ValLys: 2.165 ± 0.042	5.841ValLeu: 5.841 ± 0.068	1.691ValMet: 1.691 ± 0.034	1.988ValAsn: 1.988 ± 0.048	3.781ValPro: 3.781 ± 0.054	2.265ValGln: 2.265 ± 0.046	5.041ValArg: 5.041 ± 0.065	3.995ValSer: 3.995 ± 0.058	4.602ValThr: 4.602 ± 0.066	4.952ValVal: 4.952 ± 0.072	0.79ValTrp: 0.79 ± 0.024	1.339ValTyr: 1.339 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.446TrpAla: 1.446 ± 0.033	0.126TrpCys: 0.126 ± 0.009	0.79TrpAsp: 0.79 ± 0.027	0.587TrpGlu: 0.587 ± 0.019	0.541TrpPhe: 0.541 ± 0.023	0.969TrpGly: 0.969 ± 0.025	0.374TrpHis: 0.374 ± 0.015	0.704TrpIle: 0.704 ± 0.025	0.504TrpLys: 0.504 ± 0.02	1.764TrpLeu: 1.764 ± 0.035	0.411TrpMet: 0.411 ± 0.016	0.489TrpAsn: 0.489 ± 0.02	0.735TrpPro: 0.735 ± 0.021	0.626TrpGln: 0.626 ± 0.023	1.359TrpArg: 1.359 ± 0.036	0.948TrpSer: 0.948 ± 0.03	0.935TrpThr: 0.935 ± 0.023	0.871TrpVal: 0.871 ± 0.028	0.262TrpTrp: 0.262 ± 0.015	0.318TrpTyr: 0.318 ± 0.014	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.783TyrAla: 2.783 ± 0.047	0.229TyrCys: 0.229 ± 0.013	1.735TyrAsp: 1.735 ± 0.046	1.146TyrGlu: 1.146 ± 0.032	0.893TyrPhe: 0.893 ± 0.026	2.292TyrGly: 2.292 ± 0.047	0.572TyrHis: 0.572 ± 0.022	0.91TyrIle: 0.91 ± 0.024	0.655TyrLys: 0.655 ± 0.024	2.194TyrLeu: 2.194 ± 0.036	0.492TyrMet: 0.492 ± 0.017	0.654TyrAsn: 0.654 ± 0.025	1.089TyrPro: 1.089 ± 0.029	0.789TyrGln: 0.789 ± 0.025	2.018TyrArg: 2.018 ± 0.04	1.243TyrSer: 1.243 ± 0.032	1.032TyrThr: 1.032 ± 0.031	1.659TyrVal: 1.659 ± 0.038	0.366TyrTrp: 0.366 ± 0.015	0.735TyrTyr: 0.735 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4284 proteins (1403714 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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