Amino acid dipepetide frequency for Anaeromassilibacillus sp. An200

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.043AlaAla: 11.043 ± 0.139	1.546AlaCys: 1.546 ± 0.045	4.775AlaAsp: 4.775 ± 0.077	6.258AlaGlu: 6.258 ± 0.085	3.44AlaPhe: 3.44 ± 0.057	6.999AlaGly: 6.999 ± 0.1	1.469AlaHis: 1.469 ± 0.039	4.576AlaIle: 4.576 ± 0.073	4.0AlaLys: 4.0 ± 0.071	9.012AlaLeu: 9.012 ± 0.112	2.592AlaMet: 2.592 ± 0.061	2.475AlaAsn: 2.475 ± 0.046	3.267AlaPro: 3.267 ± 0.07	3.592AlaGln: 3.592 ± 0.063	4.725AlaArg: 4.725 ± 0.069	4.92AlaSer: 4.92 ± 0.076	3.1AlaThr: 3.1 ± 0.06	7.704AlaVal: 7.704 ± 0.102	0.87AlaTrp: 0.87 ± 0.032	2.726AlaTyr: 2.726 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	1.553CysAla: 1.553 ± 0.042	0.374CysCys: 0.374 ± 0.019	0.882CysAsp: 0.882 ± 0.029	1.062CysGlu: 1.062 ± 0.038	0.7CysPhe: 0.7 ± 0.027	1.795CysGly: 1.795 ± 0.041	0.321CysHis: 0.321 ± 0.017	0.851CysIle: 0.851 ± 0.027	0.618CysLys: 0.618 ± 0.025	1.456CysLeu: 1.456 ± 0.037	0.425CysMet: 0.425 ± 0.02	0.475CysAsn: 0.475 ± 0.02	0.885CysPro: 0.885 ± 0.033	0.477CysGln: 0.477 ± 0.023	1.218CysArg: 1.218 ± 0.036	1.073CysSer: 1.073 ± 0.031	0.922CysThr: 0.922 ± 0.032	1.238CysVal: 1.238 ± 0.033	0.175CysTrp: 0.175 ± 0.013	0.541CysTyr: 0.541 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	4.764AspAla: 4.764 ± 0.065	0.938AspCys: 0.938 ± 0.029	2.517AspAsp: 2.517 ± 0.057	3.971AspGlu: 3.971 ± 0.061	2.666AspPhe: 2.666 ± 0.05	4.761AspGly: 4.761 ± 0.076	0.945AspHis: 0.945 ± 0.036	3.014AspIle: 3.014 ± 0.057	2.329AspLys: 2.329 ± 0.046	4.83AspLeu: 4.83 ± 0.065	1.412AspMet: 1.412 ± 0.035	1.662AspAsn: 1.662 ± 0.042	2.689AspPro: 2.689 ± 0.056	1.424AspGln: 1.424 ± 0.03	3.101AspArg: 3.101 ± 0.06	3.069AspSer: 3.069 ± 0.049	2.874AspThr: 2.874 ± 0.048	3.475AspVal: 3.475 ± 0.056	0.771AspTrp: 0.771 ± 0.023	2.237AspTyr: 2.237 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	5.92GluAla: 5.92 ± 0.088	0.777GluCys: 0.777 ± 0.028	3.777GluAsp: 3.777 ± 0.058	6.852GluGlu: 6.852 ± 0.102	2.324GluPhe: 2.324 ± 0.053	4.71GluGly: 4.71 ± 0.061	1.449GluHis: 1.449 ± 0.041	4.776GluIle: 4.776 ± 0.072	5.154GluLys: 5.154 ± 0.082	7.194GluLeu: 7.194 ± 0.098	2.102GluMet: 2.102 ± 0.039	3.448GluAsn: 3.448 ± 0.056	2.583GluPro: 2.583 ± 0.052	3.562GluGln: 3.562 ± 0.065	4.404GluArg: 4.404 ± 0.075	3.695GluSer: 3.695 ± 0.062	4.11GluThr: 4.11 ± 0.063	3.787GluVal: 3.787 ± 0.07	0.768GluTrp: 0.768 ± 0.026	2.589GluTyr: 2.589 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	3.521PheAla: 3.521 ± 0.064	0.922PheCys: 0.922 ± 0.032	2.403PheAsp: 2.403 ± 0.046	2.447PheGlu: 2.447 ± 0.049	2.039PhePhe: 2.039 ± 0.056	3.04PheGly: 3.04 ± 0.062	0.898PheHis: 0.898 ± 0.03	1.912PheIle: 1.912 ± 0.047	1.219PheLys: 1.219 ± 0.036	4.539PheLeu: 4.539 ± 0.08	0.834PheMet: 0.834 ± 0.029	1.133PheAsn: 1.133 ± 0.034	1.768PhePro: 1.768 ± 0.047	1.367PheGln: 1.367 ± 0.038	2.33PheArg: 2.33 ± 0.045	3.423PheSer: 3.423 ± 0.066	2.373PheThr: 2.373 ± 0.051	2.637PheVal: 2.637 ± 0.051	0.484PheTrp: 0.484 ± 0.022	1.545PheTyr: 1.545 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	6.028GlyAla: 6.028 ± 0.095	1.437GlyCys: 1.437 ± 0.038	3.81GlyAsp: 3.81 ± 0.058	5.469GlyGlu: 5.469 ± 0.081	2.946GlyPhe: 2.946 ± 0.054	5.788GlyGly: 5.788 ± 0.095	1.285GlyHis: 1.285 ± 0.038	4.73GlyIle: 4.73 ± 0.079	4.678GlyLys: 4.678 ± 0.071	6.524GlyLeu: 6.524 ± 0.082	2.443GlyMet: 2.443 ± 0.051	2.662GlyAsn: 2.662 ± 0.055	1.795GlyPro: 1.795 ± 0.045	2.357GlyGln: 2.357 ± 0.048	4.112GlyArg: 4.112 ± 0.07	4.446GlySer: 4.446 ± 0.069	4.293GlyThr: 4.293 ± 0.074	5.496GlyVal: 5.496 ± 0.072	0.955GlyTrp: 0.955 ± 0.031	2.92GlyTyr: 2.92 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.48HisAla: 1.48 ± 0.039	0.368HisCys: 0.368 ± 0.02	0.959HisAsp: 0.959 ± 0.032	1.104HisGlu: 1.104 ± 0.035	0.93HisPhe: 0.93 ± 0.033	1.58HisGly: 1.58 ± 0.043	0.448HisHis: 0.448 ± 0.018	1.182HisIle: 1.182 ± 0.031	0.67HisLys: 0.67 ± 0.026	1.758HisLeu: 1.758 ± 0.044	0.453HisMet: 0.453 ± 0.019	0.638HisAsn: 0.638 ± 0.024	1.161HisPro: 1.161 ± 0.034	0.587HisGln: 0.587 ± 0.024	1.113HisArg: 1.113 ± 0.037	1.068HisSer: 1.068 ± 0.03	1.096HisThr: 1.096 ± 0.037	1.166HisVal: 1.166 ± 0.036	0.244HisTrp: 0.244 ± 0.014	0.749HisTyr: 0.749 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.126IleAla: 5.126 ± 0.079	1.126IleCys: 1.126 ± 0.028	3.297IleAsp: 3.297 ± 0.065	3.373IleGlu: 3.373 ± 0.066	2.151IlePhe: 2.151 ± 0.051	4.035IleGly: 4.035 ± 0.063	1.184IleHis: 1.184 ± 0.038	2.739IleIle: 2.739 ± 0.063	2.083IleLys: 2.083 ± 0.049	5.992IleLeu: 5.992 ± 0.079	1.218IleMet: 1.218 ± 0.038	1.822IleAsn: 1.822 ± 0.043	3.122IlePro: 3.122 ± 0.056	2.024IleGln: 2.024 ± 0.05	3.73IleArg: 3.73 ± 0.063	4.023IleSer: 4.023 ± 0.067	3.245IleThr: 3.245 ± 0.061	3.892IleVal: 3.892 ± 0.06	0.56IleTrp: 0.56 ± 0.021	1.761IleTyr: 1.761 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	4.38LysAla: 4.38 ± 0.076	0.532LysCys: 0.532 ± 0.025	2.422LysAsp: 2.422 ± 0.049	4.286LysGlu: 4.286 ± 0.079	1.297LysPhe: 1.297 ± 0.036	3.134LysGly: 3.134 ± 0.054	0.778LysHis: 0.778 ± 0.027	3.001LysIle: 3.001 ± 0.066	3.716LysLys: 3.716 ± 0.078	4.414LysLeu: 4.414 ± 0.062	1.365LysMet: 1.365 ± 0.038	2.2LysAsn: 2.2 ± 0.055	1.982LysPro: 1.982 ± 0.046	2.004LysGln: 2.004 ± 0.047	2.928LysArg: 2.928 ± 0.048	2.58LysSer: 2.58 ± 0.049	2.901LysThr: 2.901 ± 0.057	2.66LysVal: 2.66 ± 0.056	0.504LysTrp: 0.504 ± 0.02	1.721LysTyr: 1.721 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	8.62LeuAla: 8.62 ± 0.115	2.164LeuCys: 2.164 ± 0.044	5.415LeuAsp: 5.415 ± 0.066	6.487LeuGlu: 6.487 ± 0.08	4.347LeuPhe: 4.347 ± 0.084	6.294LeuGly: 6.294 ± 0.103	2.007LeuHis: 2.007 ± 0.049	5.086LeuIle: 5.086 ± 0.078	4.24LeuLys: 4.24 ± 0.068	10.878LeuLeu: 10.878 ± 0.155	2.455LeuMet: 2.455 ± 0.045	3.165LeuAsn: 3.165 ± 0.058	5.013LeuPro: 5.013 ± 0.081	3.13LeuGln: 3.13 ± 0.053	6.37LeuArg: 6.37 ± 0.099	7.431LeuSer: 7.431 ± 0.102	5.79LeuThr: 5.79 ± 0.068	5.704LeuVal: 5.704 ± 0.083	1.034LeuTrp: 1.034 ± 0.036	3.328LeuTyr: 3.328 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	2.527MetAla: 2.527 ± 0.043	0.318MetCys: 0.318 ± 0.016	1.572MetAsp: 1.572 ± 0.042	2.327MetGlu: 2.327 ± 0.047	0.812MetPhe: 0.812 ± 0.027	1.897MetGly: 1.897 ± 0.045	0.416MetHis: 0.416 ± 0.018	1.427MetIle: 1.427 ± 0.04	1.77MetLys: 1.77 ± 0.047	2.551MetLeu: 2.551 ± 0.049	0.835MetMet: 0.835 ± 0.029	1.136MetAsn: 1.136 ± 0.034	1.15MetPro: 1.15 ± 0.032	1.1MetGln: 1.1 ± 0.032	1.511MetArg: 1.511 ± 0.037	1.483MetSer: 1.483 ± 0.037	1.587MetThr: 1.587 ± 0.036	1.68MetVal: 1.68 ± 0.036	0.201MetTrp: 0.201 ± 0.013	0.646MetTyr: 0.646 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.954AsnAla: 2.954 ± 0.061	0.531AsnCys: 0.531 ± 0.024	1.675AsnAsp: 1.675 ± 0.045	2.147AsnGlu: 2.147 ± 0.045	1.355AsnPhe: 1.355 ± 0.029	3.248AsnGly: 3.248 ± 0.067	0.659AsnHis: 0.659 ± 0.028	2.224AsnIle: 2.224 ± 0.048	1.386AsnLys: 1.386 ± 0.033	3.268AsnLeu: 3.268 ± 0.056	0.943AsnMet: 0.943 ± 0.029	1.126AsnAsn: 1.126 ± 0.039	1.966AsnPro: 1.966 ± 0.045	1.242AsnGln: 1.242 ± 0.04	2.079AsnArg: 2.079 ± 0.049	1.944AsnSer: 1.944 ± 0.043	1.915AsnThr: 1.915 ± 0.046	2.196AsnVal: 2.196 ± 0.045	0.383AsnTrp: 0.383 ± 0.021	1.218AsnTyr: 1.218 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	3.825ProAla: 3.825 ± 0.08	0.664ProCys: 0.664 ± 0.028	3.019ProAsp: 3.019 ± 0.057	4.243ProGlu: 4.243 ± 0.077	1.906ProPhe: 1.906 ± 0.04	3.13ProGly: 3.13 ± 0.067	0.801ProHis: 0.801 ± 0.029	2.106ProIle: 2.106 ± 0.04	1.777ProLys: 1.777 ± 0.043	3.694ProLeu: 3.694 ± 0.065	1.003ProMet: 1.003 ± 0.032	1.379ProAsn: 1.379 ± 0.04	1.57ProPro: 1.57 ± 0.045	1.77ProGln: 1.77 ± 0.043	1.791ProArg: 1.791 ± 0.039	2.519ProSer: 2.519 ± 0.055	1.87ProThr: 1.87 ± 0.041	3.625ProVal: 3.625 ± 0.058	0.479ProTrp: 0.479 ± 0.02	1.498ProTyr: 1.498 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	3.571GlnAla: 3.571 ± 0.066	0.407GlnCys: 0.407 ± 0.018	1.676GlnAsp: 1.676 ± 0.041	3.348GlnGlu: 3.348 ± 0.073	1.173GlnPhe: 1.173 ± 0.028	2.29GlnGly: 2.29 ± 0.05	0.555GlnHis: 0.555 ± 0.025	2.343GlnIle: 2.343 ± 0.047	2.458GlnLys: 2.458 ± 0.054	3.202GlnLeu: 3.202 ± 0.055	1.113GlnMet: 1.113 ± 0.034	1.68GlnAsn: 1.68 ± 0.048	1.424GlnPro: 1.424 ± 0.044	1.62GlnGln: 1.62 ± 0.05	2.1GlnArg: 2.1 ± 0.045	1.949GlnSer: 1.949 ± 0.045	2.066GlnThr: 2.066 ± 0.053	2.02GlnVal: 2.02 ± 0.043	0.408GlnTrp: 0.408 ± 0.017	1.49GlnTyr: 1.49 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	4.71ArgAla: 4.71 ± 0.075	0.961ArgCys: 0.961 ± 0.031	2.934ArgAsp: 2.934 ± 0.057	5.256ArgGlu: 5.256 ± 0.093	2.643ArgPhe: 2.643 ± 0.052	3.512ArgGly: 3.512 ± 0.058	1.13ArgHis: 1.13 ± 0.038	3.55ArgIle: 3.55 ± 0.055	3.134ArgLys: 3.134 ± 0.064	6.146ArgLeu: 6.146 ± 0.085	1.761ArgMet: 1.761 ± 0.04	1.851ArgAsn: 1.851 ± 0.042	2.097ArgPro: 2.097 ± 0.048	2.271ArgGln: 2.271 ± 0.048	4.363ArgArg: 4.363 ± 0.09	3.037ArgSer: 3.037 ± 0.056	2.883ArgThr: 2.883 ± 0.061	3.71ArgVal: 3.71 ± 0.058	0.716ArgTrp: 0.716 ± 0.026	2.196ArgTyr: 2.196 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	5.335SerAla: 5.335 ± 0.083	1.063SerCys: 1.063 ± 0.031	3.048SerAsp: 3.048 ± 0.049	3.792SerGlu: 3.792 ± 0.067	2.998SerPhe: 2.998 ± 0.049	5.521SerGly: 5.521 ± 0.078	1.195SerHis: 1.195 ± 0.036	3.441SerIle: 3.441 ± 0.063	2.294SerLys: 2.294 ± 0.051	6.3SerLeu: 6.3 ± 0.09	1.723SerMet: 1.723 ± 0.044	1.765SerAsn: 1.765 ± 0.045	2.572SerPro: 2.572 ± 0.053	2.144SerGln: 2.144 ± 0.055	3.547SerArg: 3.547 ± 0.06	4.082SerSer: 4.082 ± 0.084	3.041SerThr: 3.041 ± 0.059	4.558SerVal: 4.558 ± 0.069	0.627SerTrp: 0.627 ± 0.027	2.181SerTyr: 2.181 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	5.28ThrAla: 5.28 ± 0.078	0.665ThrCys: 0.665 ± 0.025	2.91ThrAsp: 2.91 ± 0.056	3.601ThrGlu: 3.601 ± 0.064	2.059ThrPhe: 2.059 ± 0.046	4.787ThrGly: 4.787 ± 0.074	0.943ThrHis: 0.943 ± 0.031	3.199ThrIle: 3.199 ± 0.058	2.147ThrLys: 2.147 ± 0.045	5.341ThrLeu: 5.341 ± 0.075	1.368ThrMet: 1.368 ± 0.039	1.652ThrAsn: 1.652 ± 0.049	2.838ThrPro: 2.838 ± 0.053	1.857ThrGln: 1.857 ± 0.039	2.5ThrArg: 2.5 ± 0.051	2.824ThrSer: 2.824 ± 0.063	2.629ThrThr: 2.629 ± 0.058	4.625ThrVal: 4.625 ± 0.074	0.495ThrTrp: 0.495 ± 0.023	1.722ThrTyr: 1.722 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	4.771ValAla: 4.771 ± 0.073	1.42ValCys: 1.42 ± 0.037	3.54ValAsp: 3.54 ± 0.064	4.507ValGlu: 4.507 ± 0.067	2.957ValPhe: 2.957 ± 0.057	4.19ValGly: 4.19 ± 0.069	1.176ValHis: 1.176 ± 0.033	3.952ValIle: 3.952 ± 0.062	3.146ValLys: 3.146 ± 0.059	7.146ValLeu: 7.146 ± 0.098	1.802ValMet: 1.802 ± 0.04	2.368ValAsn: 2.368 ± 0.046	3.001ValPro: 3.001 ± 0.059	2.404ValGln: 2.404 ± 0.048	3.998ValArg: 3.998 ± 0.067	5.071ValSer: 5.071 ± 0.087	4.188ValThr: 4.188 ± 0.082	4.625ValVal: 4.625 ± 0.07	0.825ValTrp: 0.825 ± 0.026	2.434ValTyr: 2.434 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.735TrpAla: 0.735 ± 0.025	0.198TrpCys: 0.198 ± 0.015	0.642TrpAsp: 0.642 ± 0.029	0.944TrpGlu: 0.944 ± 0.031	0.495TrpPhe: 0.495 ± 0.019	0.789TrpGly: 0.789 ± 0.024	0.249TrpHis: 0.249 ± 0.015	0.629TrpIle: 0.629 ± 0.024	0.691TrpLys: 0.691 ± 0.032	1.089TrpLeu: 1.089 ± 0.038	0.344TrpMet: 0.344 ± 0.02	0.558TrpAsn: 0.558 ± 0.024	0.324TrpPro: 0.324 ± 0.017	0.52TrpGln: 0.52 ± 0.023	0.591TrpArg: 0.591 ± 0.024	0.588TrpSer: 0.588 ± 0.024	0.504TrpThr: 0.504 ± 0.022	0.586TrpVal: 0.586 ± 0.023	0.134TrpTrp: 0.134 ± 0.013	0.421TrpTyr: 0.421 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.945TyrAla: 2.945 ± 0.054	0.632TyrCys: 0.632 ± 0.023	2.178TyrAsp: 2.178 ± 0.058	2.508TyrGlu: 2.508 ± 0.053	1.596TyrPhe: 1.596 ± 0.039	2.675TyrGly: 2.675 ± 0.053	0.846TyrHis: 0.846 ± 0.03	1.767TyrIle: 1.767 ± 0.047	1.295TyrLys: 1.295 ± 0.033	3.626TyrLeu: 3.626 ± 0.073	0.754TyrMet: 0.754 ± 0.026	1.264TyrAsn: 1.264 ± 0.036	1.459TyrPro: 1.459 ± 0.042	1.441TyrGln: 1.441 ± 0.039	2.353TyrArg: 2.353 ± 0.049	2.001TyrSer: 2.001 ± 0.045	2.053TyrThr: 2.053 ± 0.048	2.137TyrVal: 2.137 ± 0.047	0.394TyrTrp: 0.394 ± 0.019	1.442TyrTyr: 1.442 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3479 proteins (1072320 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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