Panicum hallii var. hallii
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 38607 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T7EAB1|A0A2T7EAB1_9POAL MFS domain-containing protein OS=Panicum hallii var. hallii OX=1504633 GN=GQ55_3G167400 PE=3 SV=1
MM1 pKa = 8.2 DD2 pKa = 4.66 CTHH5 pKa = 6.45 HH6 pKa = 6.28 QPRR9 pKa = 11.84 PTMTPNPEE17 pKa = 4.24 PSPAPAPPTAAPQEE31 pKa = 4.13 ATGDD35 pKa = 4.01 SEE37 pKa = 4.9 SDD39 pKa = 2.97 SDD41 pKa = 4.08 TTVSALLTGDD51 pKa = 3.82 HH52 pKa = 7.06 PSVPGYY58 pKa = 10.65 DD59 pKa = 4.22 DD60 pKa = 4.61 EE61 pKa = 7.83 DD62 pKa = 4.23 DD63 pKa = 5.11 AEE65 pKa = 4.48 SCSGGDD71 pKa = 3.93 GGSGLCGGATSAAGGDD87 pKa = 4.02 GSDD90 pKa = 4.06 GDD92 pKa = 4.55 DD93 pKa = 3.63 DD94 pKa = 4.27 VGAEE98 pKa = 3.84 RR99 pKa = 11.84 GEE101 pKa = 4.43 AEE103 pKa = 4.58 VDD105 pKa = 3.14 SSMAVPWWRR114 pKa = 11.84 RR115 pKa = 11.84 MAVEE119 pKa = 4.14 EE120 pKa = 3.93 AAAAAAHH127 pKa = 7.5 DD128 pKa = 4.57 DD129 pKa = 4.27 GGWFAPAAEE138 pKa = 4.49 AGVVAGAGGHH148 pKa = 5.1 SAEE151 pKa = 4.41 SNILFWEE158 pKa = 4.3 ACIEE162 pKa = 3.98 HH163 pKa = 7.12 GYY165 pKa = 10.49
Molecular weight: 16.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.528
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.062
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.859
Patrickios 1.036
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|A0A2T7EWV5|A0A2T7EWV5_9POAL Uncharacterized protein OS=Panicum hallii var. hallii OX=1504633 GN=GQ55_2G383200 PE=4 SV=1
MM1 pKa = 7.65 ARR3 pKa = 11.84 RR4 pKa = 11.84 GWARR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 GRR12 pKa = 11.84 AWPPGLARR20 pKa = 11.84 RR21 pKa = 11.84 HH22 pKa = 4.08 GWARR26 pKa = 11.84 RR27 pKa = 11.84 PAWAQRR33 pKa = 11.84 GSAQWVMARR42 pKa = 11.84 RR43 pKa = 11.84 GWARR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 GRR51 pKa = 11.84 AWPPGLARR59 pKa = 11.84 RR60 pKa = 11.84 HH61 pKa = 4.08 GWARR65 pKa = 11.84 RR66 pKa = 11.84 PAWARR71 pKa = 11.84 WPGKK75 pKa = 10.0 ARR77 pKa = 11.84 QPGKK81 pKa = 9.43 AWRR84 pKa = 11.84 RR85 pKa = 11.84 GWARR89 pKa = 11.84 RR90 pKa = 11.84 SGSLACC96 pKa = 5.39
Molecular weight: 11.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.33
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.632
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.34
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.266
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
33011
5596
38607
15483065
29
5381
401.0
44.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.453 ± 0.019
1.923 ± 0.007
5.309 ± 0.008
6.004 ± 0.014
3.617 ± 0.008
7.358 ± 0.015
2.464 ± 0.006
4.29 ± 0.009
4.867 ± 0.011
9.491 ± 0.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.372 ± 0.005
3.458 ± 0.009
5.787 ± 0.013
3.543 ± 0.01
6.483 ± 0.013
8.38 ± 0.016
4.715 ± 0.007
6.652 ± 0.009
1.296 ± 0.005
2.537 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here