Vibrio phage VP93
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3VVR0|C3VVR0_9CAUD Uncharacterized protein OS=Vibrio phage VP93 OX=641832 GN=VPP93_gp20 PE=4 SV=1
MM1 pKa = 8.21 DD2 pKa = 4.86 EE3 pKa = 4.22 KK4 pKa = 10.78 QAQNVAEE11 pKa = 4.19 QEE13 pKa = 4.56 VQVVAYY19 pKa = 10.1 NCGEE23 pKa = 4.12 GLCEE27 pKa = 3.86 LFGKK31 pKa = 9.49 IRR33 pKa = 11.84 EE34 pKa = 4.21 WAGARR39 pKa = 11.84 NILKK43 pKa = 10.21 GCTPLAQACKK53 pKa = 10.17 LSEE56 pKa = 4.09 EE57 pKa = 4.22 MNEE60 pKa = 4.09 VITAIVKK67 pKa = 10.46 GKK69 pKa = 10.33 LDD71 pKa = 3.65 SLKK74 pKa = 11.03 DD75 pKa = 3.93 GIGDD79 pKa = 3.82 SVVVLDD85 pKa = 6.11 IIAAQLGADD94 pKa = 4.0 FEE96 pKa = 5.56 DD97 pKa = 3.98 VVNTSDD103 pKa = 3.7 YY104 pKa = 10.76 TQMLAQLEE112 pKa = 4.43 AVAALHH118 pKa = 6.19 PEE120 pKa = 4.08 KK121 pKa = 10.8 NRR123 pKa = 11.84 AGIIGTLNAQTSDD136 pKa = 3.89 IIGALYY142 pKa = 10.58 NYY144 pKa = 10.28 DD145 pKa = 3.3 IDD147 pKa = 4.52 NEE149 pKa = 4.06 EE150 pKa = 4.19 SLADD154 pKa = 3.36 AVIAIEE160 pKa = 3.97 HH161 pKa = 6.32 LRR163 pKa = 11.84 RR164 pKa = 11.84 MYY166 pKa = 9.39 CTLYY170 pKa = 10.4 IIAHH174 pKa = 6.47 HH175 pKa = 6.8 YY176 pKa = 11.0 DD177 pKa = 3.15 LTLYY181 pKa = 10.34 EE182 pKa = 4.88 CVNFAYY188 pKa = 10.47 GEE190 pKa = 4.0 IKK192 pKa = 10.27 DD193 pKa = 4.24 RR194 pKa = 11.84 KK195 pKa = 9.56 GEE197 pKa = 3.88 MRR199 pKa = 11.84 DD200 pKa = 3.34 GVFVKK205 pKa = 10.47 EE206 pKa = 4.09 ADD208 pKa = 3.43 LL209 pKa = 4.38
Molecular weight: 23.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.373
IPC2_protein 4.469
IPC_protein 4.406
Toseland 4.24
ProMoST 4.52
Dawson 4.355
Bjellqvist 4.507
Wikipedia 4.228
Rodwell 4.24
Grimsley 4.151
Solomon 4.355
Lehninger 4.304
Nozaki 4.469
DTASelect 4.622
Thurlkill 4.253
EMBOSS 4.253
Sillero 4.52
Patrickios 3.3
IPC_peptide 4.355
IPC2_peptide 4.507
IPC2.peptide.svr19 4.441
Protein with the highest isoelectric point:
>tr|C3VVP9|C3VVP9_9CAUD Uncharacterized protein OS=Vibrio phage VP93 OX=641832 GN=VPP93_gp9 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.15 CCLSSGLRR10 pKa = 11.84 VNLKK14 pKa = 10.17 CLSGCRR20 pKa = 11.84 RR21 pKa = 11.84 IHH23 pKa = 5.95 YY24 pKa = 7.54 LQQVSCTWQVIRR36 pKa = 11.84 QRR38 pKa = 11.84 VVVLVKK44 pKa = 9.52 TSVVLRR50 pKa = 11.84 MTRR53 pKa = 11.84 GATLTTT59 pKa = 3.93
Molecular weight: 6.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.306
IPC2_protein 9.75
IPC_protein 10.804
Toseland 11.023
ProMoST 11.038
Dawson 11.067
Bjellqvist 10.891
Wikipedia 11.374
Rodwell 11.155
Grimsley 11.111
Solomon 11.345
Lehninger 11.286
Nozaki 11.052
DTASelect 10.877
Thurlkill 11.023
EMBOSS 11.462
Sillero 11.038
Patrickios 10.95
IPC_peptide 11.345
IPC2_peptide 10.423
IPC2.peptide.svr19 8.796
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
44
0
44
13171
30
1284
299.3
33.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.551 ± 0.614
1.359 ± 0.259
6.674 ± 0.233
6.37 ± 0.268
3.432 ± 0.157
7.091 ± 0.286
2.02 ± 0.194
4.449 ± 0.222
5.087 ± 0.295
8.838 ± 0.293
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.946 ± 0.207
4.244 ± 0.298
3.994 ± 0.226
4.252 ± 0.25
5.899 ± 0.275
5.451 ± 0.284
6.347 ± 0.231
6.818 ± 0.229
1.427 ± 0.173
3.751 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here