Amino acid dipepetide frequency for Vibrio phage VP93

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.086AlaAla: 11.086 ± 1.897
1.443AlaCys: 1.443 ± 0.487
6.454AlaAsp: 6.454 ± 0.692
6.15AlaGlu: 6.15 ± 0.83
2.961AlaPhe: 2.961 ± 0.432
5.923AlaGly: 5.923 ± 1.02
1.67AlaHis: 1.67 ± 0.409
4.176AlaIle: 4.176 ± 0.562
3.721AlaLys: 3.721 ± 0.597
9.643AlaLeu: 9.643 ± 0.694
3.417AlaMet: 3.417 ± 0.722
3.797AlaAsn: 3.797 ± 0.741
3.721AlaPro: 3.721 ± 0.489
5.315AlaGln: 5.315 ± 0.758
4.935AlaArg: 4.935 ± 0.533
4.784AlaSer: 4.784 ± 0.771
5.315AlaThr: 5.315 ± 0.838
7.213AlaVal: 7.213 ± 0.808
0.683AlaTrp: 0.683 ± 0.183
3.113AlaTyr: 3.113 ± 0.542
0.0AlaXaa: 0.0 ± 0.0
Cys
1.291CysAla: 1.291 ± 0.477
0.304CysCys: 0.304 ± 0.172
0.987CysAsp: 0.987 ± 0.367
0.532CysGlu: 0.532 ± 0.201
0.456CysPhe: 0.456 ± 0.199
0.835CysGly: 0.835 ± 0.249
0.228CysHis: 0.228 ± 0.101
0.456CysIle: 0.456 ± 0.192
0.532CysLys: 0.532 ± 0.2
1.063CysLeu: 1.063 ± 0.329
0.532CysMet: 0.532 ± 0.174
0.532CysAsn: 0.532 ± 0.207
0.835CysPro: 0.835 ± 0.287
0.607CysGln: 0.607 ± 0.154
0.987CysArg: 0.987 ± 0.331
0.38CysSer: 0.38 ± 0.155
1.215CysThr: 1.215 ± 0.291
0.911CysVal: 0.911 ± 0.305
0.38CysTrp: 0.38 ± 0.151
0.532CysTyr: 0.532 ± 0.189
0.0CysXaa: 0.0 ± 0.0
Asp
7.365AspAla: 7.365 ± 0.842
0.835AspCys: 0.835 ± 0.312
4.252AspAsp: 4.252 ± 0.56
4.328AspGlu: 4.328 ± 0.609
2.658AspPhe: 2.658 ± 0.438
6.378AspGly: 6.378 ± 0.714
0.759AspHis: 0.759 ± 0.256
3.948AspIle: 3.948 ± 0.508
3.417AspLys: 3.417 ± 0.427
5.239AspLeu: 5.239 ± 0.61
1.974AspMet: 1.974 ± 0.452
2.582AspAsn: 2.582 ± 0.366
2.582AspPro: 2.582 ± 0.539
1.215AspGln: 1.215 ± 0.227
3.265AspArg: 3.265 ± 0.582
3.721AspSer: 3.721 ± 0.53
4.48AspThr: 4.48 ± 0.641
4.48AspVal: 4.48 ± 0.663
0.759AspTrp: 0.759 ± 0.232
2.506AspTyr: 2.506 ± 0.469
0.0AspXaa: 0.0 ± 0.0
Glu
5.619GluAla: 5.619 ± 0.789
0.683GluCys: 0.683 ± 0.303
3.569GluAsp: 3.569 ± 0.569
3.797GluGlu: 3.797 ± 0.499
3.113GluPhe: 3.113 ± 0.462
3.872GluGly: 3.872 ± 0.688
2.582GluHis: 2.582 ± 0.485
2.658GluIle: 2.658 ± 0.402
3.417GluLys: 3.417 ± 0.4
6.302GluLeu: 6.302 ± 0.6
2.506GluMet: 2.506 ± 0.385
2.885GluAsn: 2.885 ± 0.51
1.291GluPro: 1.291 ± 0.263
2.885GluGln: 2.885 ± 0.409
4.176GluArg: 4.176 ± 0.513
2.733GluSer: 2.733 ± 0.433
3.417GluThr: 3.417 ± 0.508
5.011GluVal: 5.011 ± 0.54
0.911GluTrp: 0.911 ± 0.23
2.278GluTyr: 2.278 ± 0.452
0.0GluXaa: 0.0 ± 0.0
Phe
3.493PheAla: 3.493 ± 0.625
0.607PheCys: 0.607 ± 0.183
2.733PheAsp: 2.733 ± 0.489
2.809PheGlu: 2.809 ± 0.434
1.443PhePhe: 1.443 ± 0.269
2.278PheGly: 2.278 ± 0.321
0.532PheHis: 0.532 ± 0.204
1.367PheIle: 1.367 ± 0.311
1.898PheLys: 1.898 ± 0.477
2.658PheLeu: 2.658 ± 0.393
1.063PheMet: 1.063 ± 0.306
1.746PheAsn: 1.746 ± 0.391
1.291PhePro: 1.291 ± 0.334
1.367PheGln: 1.367 ± 0.283
1.746PheArg: 1.746 ± 0.426
1.898PheSer: 1.898 ± 0.294
1.746PheThr: 1.746 ± 0.342
1.746PheVal: 1.746 ± 0.352
0.456PheTrp: 0.456 ± 0.243
1.443PheTyr: 1.443 ± 0.293
0.0PheXaa: 0.0 ± 0.0
Gly
6.682GlyAla: 6.682 ± 0.919
0.683GlyCys: 0.683 ± 0.222
4.328GlyAsp: 4.328 ± 0.566
5.011GlyGlu: 5.011 ± 0.641
2.43GlyPhe: 2.43 ± 0.444
5.391GlyGly: 5.391 ± 0.617
1.063GlyHis: 1.063 ± 0.301
3.797GlyIle: 3.797 ± 0.505
5.543GlyLys: 5.543 ± 0.868
5.087GlyLeu: 5.087 ± 0.864
1.974GlyMet: 1.974 ± 0.443
2.733GlyAsn: 2.733 ± 0.75
1.519GlyPro: 1.519 ± 0.259
2.809GlyGln: 2.809 ± 0.565
4.328GlyArg: 4.328 ± 0.47
3.417GlySer: 3.417 ± 0.683
5.315GlyThr: 5.315 ± 0.69
5.315GlyVal: 5.315 ± 0.69
0.835GlyTrp: 0.835 ± 0.285
2.658GlyTyr: 2.658 ± 0.479
0.0GlyXaa: 0.0 ± 0.0
His
1.367HisAla: 1.367 ± 0.301
0.304HisCys: 0.304 ± 0.139
0.987HisAsp: 0.987 ± 0.294
1.898HisGlu: 1.898 ± 0.366
0.835HisPhe: 0.835 ± 0.223
1.595HisGly: 1.595 ± 0.319
0.456HisHis: 0.456 ± 0.217
1.367HisIle: 1.367 ± 0.248
0.911HisLys: 0.911 ± 0.209
2.126HisLeu: 2.126 ± 0.362
0.532HisMet: 0.532 ± 0.222
0.456HisAsn: 0.456 ± 0.165
1.215HisPro: 1.215 ± 0.326
0.835HisGln: 0.835 ± 0.309
1.063HisArg: 1.063 ± 0.322
0.532HisSer: 0.532 ± 0.163
1.367HisThr: 1.367 ± 0.289
1.063HisVal: 1.063 ± 0.255
0.228HisTrp: 0.228 ± 0.123
1.063HisTyr: 1.063 ± 0.32
0.0HisXaa: 0.0 ± 0.0
Ile
3.417IleAla: 3.417 ± 0.415
0.152IleCys: 0.152 ± 0.097
3.417IleAsp: 3.417 ± 0.371
3.417IleGlu: 3.417 ± 0.629
0.911IlePhe: 0.911 ± 0.309
3.189IleGly: 3.189 ± 0.44
1.519IleHis: 1.519 ± 0.251
2.506IleIle: 2.506 ± 0.451
2.506IleLys: 2.506 ± 0.45
3.037IleLeu: 3.037 ± 0.523
1.063IleMet: 1.063 ± 0.368
1.974IleAsn: 1.974 ± 0.387
2.658IlePro: 2.658 ± 0.41
1.822IleGln: 1.822 ± 0.373
2.809IleArg: 2.809 ± 0.506
2.809IleSer: 2.809 ± 0.505
2.658IleThr: 2.658 ± 0.336
2.809IleVal: 2.809 ± 0.48
0.532IleTrp: 0.532 ± 0.211
1.291IleTyr: 1.291 ± 0.439
0.0IleXaa: 0.0 ± 0.0
Lys
5.467LysAla: 5.467 ± 0.863
0.911LysCys: 0.911 ± 0.335
3.872LysAsp: 3.872 ± 0.534
2.582LysGlu: 2.582 ± 0.441
1.215LysPhe: 1.215 ± 0.296
3.417LysGly: 3.417 ± 0.464
0.911LysHis: 0.911 ± 0.273
1.443LysIle: 1.443 ± 0.317
2.278LysLys: 2.278 ± 0.497
5.543LysLeu: 5.543 ± 0.663
1.215LysMet: 1.215 ± 0.329
1.746LysAsn: 1.746 ± 0.267
2.582LysPro: 2.582 ± 0.403
2.733LysGln: 2.733 ± 0.455
3.189LysArg: 3.189 ± 0.423
2.582LysSer: 2.582 ± 0.392
3.189LysThr: 3.189 ± 0.582
2.885LysVal: 2.885 ± 0.553
1.063LysTrp: 1.063 ± 0.282
2.05LysTyr: 2.05 ± 0.308
0.0LysXaa: 0.0 ± 0.0
Leu
8.428LeuAla: 8.428 ± 1.074
1.898LeuCys: 1.898 ± 0.378
6.53LeuAsp: 6.53 ± 0.624
4.784LeuGlu: 4.784 ± 0.505
2.733LeuPhe: 2.733 ± 0.311
4.708LeuGly: 4.708 ± 0.617
1.746LeuHis: 1.746 ± 0.303
2.961LeuIle: 2.961 ± 0.534
4.252LeuLys: 4.252 ± 0.54
6.454LeuLeu: 6.454 ± 0.826
2.809LeuMet: 2.809 ± 0.569
4.48LeuAsn: 4.48 ± 0.565
3.872LeuPro: 3.872 ± 0.671
4.404LeuGln: 4.404 ± 0.631
6.682LeuArg: 6.682 ± 0.949
5.391LeuSer: 5.391 ± 0.79
6.302LeuThr: 6.302 ± 0.802
4.86LeuVal: 4.86 ± 0.639
1.063LeuTrp: 1.063 ± 0.299
4.024LeuTyr: 4.024 ± 0.561
0.0LeuXaa: 0.0 ± 0.0
Met
3.265MetAla: 3.265 ± 0.58
0.152MetCys: 0.152 ± 0.103
1.822MetAsp: 1.822 ± 0.48
1.215MetGlu: 1.215 ± 0.252
1.215MetPhe: 1.215 ± 0.264
2.278MetGly: 2.278 ± 0.429
0.532MetHis: 0.532 ± 0.184
1.063MetIle: 1.063 ± 0.304
1.063MetLys: 1.063 ± 0.346
3.417MetLeu: 3.417 ± 0.669
0.911MetMet: 0.911 ± 0.332
1.291MetAsn: 1.291 ± 0.273
1.443MetPro: 1.443 ± 0.436
1.367MetGln: 1.367 ± 0.277
1.519MetArg: 1.519 ± 0.321
1.746MetSer: 1.746 ± 0.275
1.974MetThr: 1.974 ± 0.433
1.595MetVal: 1.595 ± 0.434
0.304MetTrp: 0.304 ± 0.168
1.291MetTyr: 1.291 ± 0.239
0.0MetXaa: 0.0 ± 0.0
Asn
3.948AsnAla: 3.948 ± 0.604
0.607AsnCys: 0.607 ± 0.21
1.974AsnAsp: 1.974 ± 0.377
2.354AsnGlu: 2.354 ± 0.42
1.595AsnPhe: 1.595 ± 0.298
2.658AsnGly: 2.658 ± 0.402
0.683AsnHis: 0.683 ± 0.219
2.506AsnIle: 2.506 ± 0.379
1.822AsnLys: 1.822 ± 0.375
4.252AsnLeu: 4.252 ± 0.876
1.367AsnMet: 1.367 ± 0.32
1.974AsnAsn: 1.974 ± 0.412
2.202AsnPro: 2.202 ± 0.362
2.202AsnGln: 2.202 ± 0.587
2.885AsnArg: 2.885 ± 0.65
1.974AsnSer: 1.974 ± 0.345
2.961AsnThr: 2.961 ± 0.433
2.658AsnVal: 2.658 ± 0.438
0.683AsnTrp: 0.683 ± 0.231
1.139AsnTyr: 1.139 ± 0.381
0.0AsnXaa: 0.0 ± 0.0
Pro
3.797ProAla: 3.797 ± 1.048
0.152ProCys: 0.152 ± 0.092
3.872ProAsp: 3.872 ± 0.569
3.645ProGlu: 3.645 ± 0.529
0.987ProPhe: 0.987 ± 0.191
1.595ProGly: 1.595 ± 0.377
0.456ProHis: 0.456 ± 0.139
1.519ProIle: 1.519 ± 0.336
2.506ProLys: 2.506 ± 0.358
3.113ProLeu: 3.113 ± 0.509
0.987ProMet: 0.987 ± 0.237
1.519ProAsn: 1.519 ± 0.32
1.367ProPro: 1.367 ± 0.35
1.519ProGln: 1.519 ± 0.539
2.202ProArg: 2.202 ± 0.474
1.595ProSer: 1.595 ± 0.349
2.961ProThr: 2.961 ± 0.611
3.645ProVal: 3.645 ± 0.461
0.532ProTrp: 0.532 ± 0.184
1.974ProTyr: 1.974 ± 0.499
0.0ProXaa: 0.0 ± 0.0
Gln
4.86GlnAla: 4.86 ± 0.78
0.38GlnCys: 0.38 ± 0.167
1.898GlnAsp: 1.898 ± 0.348
2.809GlnGlu: 2.809 ± 0.442
1.595GlnPhe: 1.595 ± 0.385
3.797GlnGly: 3.797 ± 0.367
0.607GlnHis: 0.607 ± 0.198
1.746GlnIle: 1.746 ± 0.291
1.898GlnLys: 1.898 ± 0.478
3.341GlnLeu: 3.341 ± 0.374
1.291GlnMet: 1.291 ± 0.316
2.354GlnAsn: 2.354 ± 0.483
0.987GlnPro: 0.987 ± 0.564
1.443GlnGln: 1.443 ± 0.461
2.658GlnArg: 2.658 ± 0.418
3.265GlnSer: 3.265 ± 0.553
2.202GlnThr: 2.202 ± 0.518
2.582GlnVal: 2.582 ± 0.479
0.532GlnTrp: 0.532 ± 0.212
2.202GlnTyr: 2.202 ± 0.506
0.0GlnXaa: 0.0 ± 0.0
Arg
5.315ArgAla: 5.315 ± 0.99
0.835ArgCys: 0.835 ± 0.239
3.189ArgAsp: 3.189 ± 0.571
4.176ArgGlu: 4.176 ± 0.524
1.898ArgPhe: 1.898 ± 0.392
3.797ArgGly: 3.797 ± 0.446
1.443ArgHis: 1.443 ± 0.305
2.885ArgIle: 2.885 ± 0.547
3.417ArgLys: 3.417 ± 0.532
5.543ArgLeu: 5.543 ± 0.742
1.974ArgMet: 1.974 ± 0.456
2.05ArgAsn: 2.05 ± 0.315
1.67ArgPro: 1.67 ± 0.401
1.898ArgGln: 1.898 ± 0.408
4.024ArgArg: 4.024 ± 0.539
3.948ArgSer: 3.948 ± 0.609
3.341ArgThr: 3.341 ± 0.425
4.48ArgVal: 4.48 ± 0.701
0.911ArgTrp: 0.911 ± 0.332
2.202ArgTyr: 2.202 ± 0.389
0.0ArgXaa: 0.0 ± 0.0
Ser
5.771SerAla: 5.771 ± 0.442
0.683SerCys: 0.683 ± 0.382
3.645SerAsp: 3.645 ± 0.409
3.645SerGlu: 3.645 ± 0.573
2.202SerPhe: 2.202 ± 0.427
4.784SerGly: 4.784 ± 0.705
0.683SerHis: 0.683 ± 0.362
2.354SerIle: 2.354 ± 0.393
2.961SerLys: 2.961 ± 0.358
4.252SerLeu: 4.252 ± 0.549
1.063SerMet: 1.063 ± 0.287
2.354SerAsn: 2.354 ± 0.321
2.733SerPro: 2.733 ± 0.452
2.05SerGln: 2.05 ± 0.609
2.658SerArg: 2.658 ± 0.543
2.733SerSer: 2.733 ± 0.451
3.569SerThr: 3.569 ± 0.635
3.797SerVal: 3.797 ± 0.619
1.139SerTrp: 1.139 ± 0.288
1.443SerTyr: 1.443 ± 0.274
0.0SerXaa: 0.0 ± 0.0
Thr
5.163ThrAla: 5.163 ± 0.65
0.683ThrCys: 0.683 ± 0.256
5.391ThrAsp: 5.391 ± 0.635
3.037ThrGlu: 3.037 ± 0.418
2.126ThrPhe: 2.126 ± 0.367
6.226ThrGly: 6.226 ± 0.757
1.443ThrHis: 1.443 ± 0.25
2.506ThrIle: 2.506 ± 0.361
2.43ThrLys: 2.43 ± 0.441
5.923ThrLeu: 5.923 ± 0.768
1.822ThrMet: 1.822 ± 0.478
2.809ThrAsn: 2.809 ± 0.473
2.05ThrPro: 2.05 ± 0.279
3.113ThrGln: 3.113 ± 0.468
3.265ThrArg: 3.265 ± 0.519
3.569ThrSer: 3.569 ± 0.501
4.1ThrThr: 4.1 ± 0.67
4.404ThrVal: 4.404 ± 0.456
1.063ThrTrp: 1.063 ± 0.402
2.354ThrTyr: 2.354 ± 0.319
0.0ThrXaa: 0.0 ± 0.0
Val
5.998ValAla: 5.998 ± 0.575
1.291ValCys: 1.291 ± 0.439
4.48ValAsp: 4.48 ± 0.493
3.569ValGlu: 3.569 ± 0.503
2.506ValPhe: 2.506 ± 0.42
4.784ValGly: 4.784 ± 0.546
1.595ValHis: 1.595 ± 0.428
2.506ValIle: 2.506 ± 0.427
4.1ValLys: 4.1 ± 0.617
6.226ValLeu: 6.226 ± 0.628
1.67ValMet: 1.67 ± 0.399
3.113ValAsn: 3.113 ± 0.341
3.265ValPro: 3.265 ± 0.589
2.658ValGln: 2.658 ± 0.344
3.645ValArg: 3.645 ± 0.53
4.328ValSer: 4.328 ± 0.582
4.1ValThr: 4.1 ± 0.506
5.163ValVal: 5.163 ± 0.615
1.215ValTrp: 1.215 ± 0.347
1.974ValTyr: 1.974 ± 0.436
0.0ValXaa: 0.0 ± 0.0
Trp
1.139TrpAla: 1.139 ± 0.347
0.456TrpCys: 0.456 ± 0.173
1.063TrpAsp: 1.063 ± 0.239
0.911TrpGlu: 0.911 ± 0.224
0.456TrpPhe: 0.456 ± 0.173
0.987TrpGly: 0.987 ± 0.323
0.607TrpHis: 0.607 ± 0.222
0.38TrpIle: 0.38 ± 0.229
0.607TrpLys: 0.607 ± 0.171
1.822TrpLeu: 1.822 ± 0.327
0.38TrpMet: 0.38 ± 0.172
0.228TrpAsn: 0.228 ± 0.113
0.304TrpPro: 0.304 ± 0.139
0.532TrpGln: 0.532 ± 0.231
0.304TrpArg: 0.304 ± 0.162
0.532TrpSer: 0.532 ± 0.179
0.835TrpThr: 0.835 ± 0.298
1.367TrpVal: 1.367 ± 0.278
0.38TrpTrp: 0.38 ± 0.182
0.987TrpTyr: 0.987 ± 0.254
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.05TyrAla: 2.05 ± 0.496
0.532TyrCys: 0.532 ± 0.215
2.278TyrAsp: 2.278 ± 0.424
3.037TyrGlu: 3.037 ± 0.676
0.987TyrPhe: 0.987 ± 0.388
2.43TyrGly: 2.43 ± 0.596
0.683TyrHis: 0.683 ± 0.182
2.354TyrIle: 2.354 ± 0.512
1.595TyrLys: 1.595 ± 0.341
3.341TyrLeu: 3.341 ± 0.516
0.835TyrMet: 0.835 ± 0.269
1.822TyrAsn: 1.822 ± 0.382
2.354TyrPro: 2.354 ± 0.378
1.746TyrGln: 1.746 ± 0.457
2.658TyrArg: 2.658 ± 0.563
2.582TyrSer: 2.582 ± 0.327
2.43TyrThr: 2.43 ± 0.452
2.202TyrVal: 2.202 ± 0.349
0.607TyrTrp: 0.607 ± 0.182
0.987TyrTyr: 0.987 ± 0.295
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (13171 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski