Desulfotomaculum arcticum DSM 17038
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5051 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I2PJJ3|A0A1I2PJJ3_9FIRM ABC-2 type transport system permease protein OS=Desulfotomaculum arcticum DSM 17038 OX=1121424 GN=SAMN05660649_00907 PE=4 SV=1
MM1 pKa = 7.23 YY2 pKa = 10.32 IFVKK6 pKa = 10.48 GYY8 pKa = 10.71 NLAKK12 pKa = 10.38 DD13 pKa = 3.51 RR14 pKa = 11.84 NGRR17 pKa = 11.84 LINIIVRR24 pKa = 11.84 TLRR27 pKa = 11.84 KK28 pKa = 9.61 YY29 pKa = 7.68 IHH31 pKa = 7.01 KK32 pKa = 10.61 YY33 pKa = 10.45 GILFLLLCLFSTMTACEE50 pKa = 4.24 SKK52 pKa = 10.81 DD53 pKa = 3.15 ILGDD57 pKa = 3.76 DD58 pKa = 4.11 EE59 pKa = 5.84 PEE61 pKa = 4.0 VSQNATEE68 pKa = 4.68 DD69 pKa = 3.47 GTMGSSNDD77 pKa = 3.69 GVNTEE82 pKa = 4.06 ADD84 pKa = 3.26 KK85 pKa = 11.38 GAEE88 pKa = 3.83 QSTAPNEE95 pKa = 4.22 LEE97 pKa = 4.33 DD98 pKa = 4.14 TVSGQDD104 pKa = 3.96 GEE106 pKa = 4.38 EE107 pKa = 4.12 TQMNEE112 pKa = 3.52 ADD114 pKa = 3.65 IDD116 pKa = 3.85 RR117 pKa = 11.84 EE118 pKa = 4.27 AMMNEE123 pKa = 4.07 YY124 pKa = 10.69 KK125 pKa = 10.76 SILKK129 pKa = 10.36 GIYY132 pKa = 9.44 DD133 pKa = 3.5 NHH135 pKa = 6.7 IFPDD139 pKa = 4.37 GQACDD144 pKa = 3.13 WDD146 pKa = 3.92 GRR148 pKa = 11.84 SDD150 pKa = 3.44 VTQNQFAVYY159 pKa = 10.22 DD160 pKa = 4.02 IDD162 pKa = 5.61 QDD164 pKa = 4.09 TQDD167 pKa = 4.34 EE168 pKa = 5.12 LILAYY173 pKa = 9.41 TNSSMAGMIEE183 pKa = 5.15 LIYY186 pKa = 11.14 DD187 pKa = 3.95 FDD189 pKa = 5.41 NDD191 pKa = 3.38 TKK193 pKa = 10.56 TANEE197 pKa = 4.12 EE198 pKa = 3.85 FRR200 pKa = 11.84 EE201 pKa = 4.46 FPTLTYY207 pKa = 10.19 YY208 pKa = 11.24 DD209 pKa = 3.58 NGIIKK214 pKa = 10.32 ADD216 pKa = 3.23 WSHH219 pKa = 5.59 NQGLAGDD226 pKa = 4.19 FWPYY230 pKa = 10.32 TLYY233 pKa = 10.77 QYY235 pKa = 11.03 DD236 pKa = 4.35 KK237 pKa = 11.11 KK238 pKa = 10.93 SDD240 pKa = 3.6 SYY242 pKa = 11.84 VDD244 pKa = 3.4 VGMVDD249 pKa = 2.56 AWDD252 pKa = 4.23 RR253 pKa = 11.84 SLSATDD259 pKa = 3.7 YY260 pKa = 11.4 NGDD263 pKa = 3.85 PFPAEE268 pKa = 4.22 SDD270 pKa = 3.3 TDD272 pKa = 3.76 GDD274 pKa = 4.34 GIVYY278 pKa = 10.27 YY279 pKa = 10.31 IMQDD283 pKa = 3.06 GEE285 pKa = 4.6 YY286 pKa = 10.61 KK287 pKa = 10.23 LDD289 pKa = 3.69 HH290 pKa = 7.17 PIDD293 pKa = 3.96 GAEE296 pKa = 3.87 YY297 pKa = 8.46 EE298 pKa = 4.16 QWRR301 pKa = 11.84 DD302 pKa = 3.46 FYY304 pKa = 10.92 IGNAKK309 pKa = 9.28 EE310 pKa = 3.97 VNIPFINLTEE320 pKa = 4.02 EE321 pKa = 4.45 NIDD324 pKa = 3.97 NIQQ327 pKa = 3.01
Molecular weight: 37.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.872
IPC_protein 3.897
Toseland 3.681
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.24
Thurlkill 3.719
EMBOSS 3.821
Sillero 4.012
Patrickios 1.914
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|A0A1I2ZC58|A0A1I2ZC58_9FIRM Transposase DDE domain-containing protein (Fragment) OS=Desulfotomaculum arcticum DSM 17038 OX=1121424 GN=SAMN05660649_04876 PE=4 SV=1
MM1 pKa = 7.68 PKK3 pKa = 9.97 IKK5 pKa = 8.1 THH7 pKa = 6.4 RR8 pKa = 11.84 GAAKK12 pKa = 10.0 RR13 pKa = 11.84 FKK15 pKa = 9.91 KK16 pKa = 9.16 TGSGKK21 pKa = 10.45 FKK23 pKa = 10.02 TSHH26 pKa = 6.54 AFHH29 pKa = 6.38 SHH31 pKa = 5.49 ILGKK35 pKa = 8.81 KK36 pKa = 5.69 TPKK39 pKa = 9.96 RR40 pKa = 11.84 KK41 pKa = 9.57 RR42 pKa = 11.84 KK43 pKa = 9.1 LRR45 pKa = 11.84 KK46 pKa = 9.37 ALIVHH51 pKa = 6.96 PANAANLQRR60 pKa = 11.84 LLPP63 pKa = 4.35
Molecular weight: 7.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.445
IPC2_protein 11.038
IPC_protein 12.544
Toseland 12.735
ProMoST 13.203
Dawson 12.735
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.749
Grimsley 12.778
Solomon 13.217
Lehninger 13.115
Nozaki 12.735
DTASelect 12.705
Thurlkill 12.735
EMBOSS 13.232
Sillero 12.735
Patrickios 12.486
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 8.972
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5051
0
5051
1486842
39
3953
294.4
32.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.343 ± 0.037
1.232 ± 0.018
5.067 ± 0.034
6.55 ± 0.032
3.915 ± 0.026
7.754 ± 0.03
1.738 ± 0.017
7.329 ± 0.031
6.026 ± 0.028
10.057 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.718 ± 0.018
4.374 ± 0.027
4.133 ± 0.02
3.511 ± 0.024
5.002 ± 0.032
5.466 ± 0.023
5.091 ± 0.032
7.347 ± 0.033
1.015 ± 0.014
3.331 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here