Vibrio phage VEJphi

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Fibrovirus; unclassified Fibrovirus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C3W4N9|C3W4N9_9VIRU Putative minor capsid protein OS=Vibrio phage VEJphi OX=642111 PE=4 SV=1
MM1 pKa = 8.22VMFMRR6 pKa = 11.84KK7 pKa = 8.88FFIISLVISLYY18 pKa = 9.72FTVLPAFAAYY28 pKa = 9.47QLPFDD33 pKa = 3.86QTKK36 pKa = 8.61TFPTVQAAAEE46 pKa = 4.38YY47 pKa = 9.69YY48 pKa = 10.96ANLLGGTSCKK58 pKa = 10.4ADD60 pKa = 3.16GNRR63 pKa = 11.84FKK65 pKa = 10.61IYY67 pKa = 9.57RR68 pKa = 11.84VKK70 pKa = 10.74SISDD74 pKa = 3.5PSFVIQQEE82 pKa = 4.58TYY84 pKa = 11.16LDD86 pKa = 3.67NKK88 pKa = 10.05CQVFSSKK95 pKa = 11.03GDD97 pKa = 3.25ISVTLIVVDD106 pKa = 5.71DD107 pKa = 4.16PTTCEE112 pKa = 3.7ASKK115 pKa = 10.83GQTGKK120 pKa = 10.93VGWNSYY126 pKa = 7.76FWGSATPSRR135 pKa = 11.84YY136 pKa = 9.22ICSSANGGCVALTGDD151 pKa = 4.48HH152 pKa = 6.64LCINIDD158 pKa = 3.65PEE160 pKa = 4.19ALKK163 pKa = 10.6NDD165 pKa = 3.8PSLYY169 pKa = 10.79DD170 pKa = 3.68CDD172 pKa = 3.91ALYY175 pKa = 10.18IVQDD179 pKa = 4.39TPCSPSGDD187 pKa = 3.77YY188 pKa = 10.5PFCTDD193 pKa = 3.63EE194 pKa = 4.45NCSSFLPEE202 pKa = 4.96PNPNPNPEE210 pKa = 4.47PEE212 pKa = 4.8PEE214 pKa = 4.76PEE216 pKa = 4.01PQPDD220 pKa = 3.99PEE222 pKa = 5.19HH223 pKa = 6.61NPSDD227 pKa = 3.78PTAPLPDD234 pKa = 3.67SGGEE238 pKa = 4.12VINPSVPPKK247 pKa = 10.39PPTEE251 pKa = 4.16EE252 pKa = 3.87KK253 pKa = 10.28PKK255 pKa = 10.44PDD257 pKa = 3.82VEE259 pKa = 4.76TPDD262 pKa = 3.77PTPDD266 pKa = 3.45SNSDD270 pKa = 3.38VVQSVTGMNEE280 pKa = 3.81DD281 pKa = 3.54MNEE284 pKa = 3.69LLTRR288 pKa = 11.84LNSDD292 pKa = 3.45NNKK295 pKa = 9.66QLDD298 pKa = 3.95DD299 pKa = 4.12VNNQLLQLNTQSQRR313 pKa = 11.84IVAQIAKK320 pKa = 9.05QEE322 pKa = 4.11KK323 pKa = 8.44QDD325 pKa = 3.42AAIYY329 pKa = 9.41EE330 pKa = 4.23NTKK333 pKa = 11.01ALIQNLNKK341 pKa = 10.62DD342 pKa = 3.06VTTAVNKK349 pKa = 6.75TTNAVNALGSKK360 pKa = 10.33VDD362 pKa = 3.84GLSDD366 pKa = 3.48AVDD369 pKa = 3.62GLGEE373 pKa = 4.32DD374 pKa = 3.59VSAIKK379 pKa = 10.5DD380 pKa = 3.59AITNVDD386 pKa = 3.12TSGAGISGTCIEE398 pKa = 5.16SDD400 pKa = 3.5TCTGFYY406 pKa = 10.59EE407 pKa = 4.24SGYY410 pKa = 9.37PDD412 pKa = 5.33GISGIFTEE420 pKa = 5.44HH421 pKa = 7.08FDD423 pKa = 3.95TVSKK427 pKa = 11.01SVTDD431 pKa = 3.68TVKK434 pKa = 11.13DD435 pKa = 3.93FMKK438 pKa = 10.12IDD440 pKa = 3.92LSHH443 pKa = 6.56AQRR446 pKa = 11.84PSFSIPVLHH455 pKa = 7.04FGNFSFDD462 pKa = 4.17DD463 pKa = 4.45YY464 pKa = 11.88INLDD468 pKa = 3.69WIFGFVRR475 pKa = 11.84VCMMVSTAFLCRR487 pKa = 11.84KK488 pKa = 9.77IIFGGG493 pKa = 3.5

Molecular weight: 53.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C3W4P2|C3W4P2_9VIRU Putative regulator protein OS=Vibrio phage VEJphi OX=642111 PE=4 SV=1
MM1 pKa = 7.7LIKK4 pKa = 10.73LPILGVLSMNRR15 pKa = 11.84SEE17 pKa = 4.0MTKK20 pKa = 9.96NFVFRR25 pKa = 11.84EE26 pKa = 4.35FEE28 pKa = 4.32CGLSVEE34 pKa = 4.72EE35 pKa = 4.5AAKK38 pKa = 10.83LCFKK42 pKa = 9.77TVNEE46 pKa = 4.24VKK48 pKa = 10.31QWDD51 pKa = 4.03AGEE54 pKa = 4.75KK55 pKa = 9.98IPPICKK61 pKa = 9.68RR62 pKa = 11.84LMRR65 pKa = 11.84WHH67 pKa = 6.19SRR69 pKa = 11.84KK70 pKa = 9.28EE71 pKa = 3.83LYY73 pKa = 10.74YY74 pKa = 10.93GDD76 pKa = 4.06EE77 pKa = 3.55WWGFRR82 pKa = 11.84MEE84 pKa = 4.38GGRR87 pKa = 11.84LIFPTGDD94 pKa = 2.67RR95 pKa = 11.84VAPQQLLAAIAILQIQAPDD114 pKa = 3.52DD115 pKa = 3.8AMTRR119 pKa = 11.84SKK121 pKa = 10.76LLKK124 pKa = 9.49YY125 pKa = 9.56ARR127 pKa = 11.84AMARR131 pKa = 11.84IKK133 pKa = 10.59GIKK136 pKa = 8.89

Molecular weight: 15.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

1936

29

493

176.0

19.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.076 ± 0.877

1.963 ± 0.479

5.733 ± 0.848

5.01 ± 0.566

5.01 ± 0.327

6.198 ± 0.494

1.55 ± 0.399

8.368 ± 0.982

6.25 ± 0.559

8.626 ± 1.008

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.738 ± 0.422

4.545 ± 0.769

5.475 ± 0.71

3.564 ± 0.382

4.184 ± 0.864

6.353 ± 0.83

5.94 ± 0.669

5.785 ± 0.549

1.446 ± 0.407

4.184 ± 0.778
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski