Amino acid dipepetide frequency for Vibrio phage VEJphi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.202AlaAla: 6.202 ± 1.883
0.0AlaCys: 0.0 ± 0.0
2.067AlaAsp: 2.067 ± 0.77
3.618AlaGlu: 3.618 ± 0.808
4.651AlaPhe: 4.651 ± 0.902
3.618AlaGly: 3.618 ± 1.519
1.034AlaHis: 1.034 ± 0.668
6.202AlaIle: 6.202 ± 1.285
4.651AlaLys: 4.651 ± 1.437
9.819AlaLeu: 9.819 ± 3.077
3.101AlaMet: 3.101 ± 1.381
2.584AlaAsn: 2.584 ± 0.845
3.101AlaPro: 3.101 ± 1.071
4.134AlaGln: 4.134 ± 1.009
2.067AlaArg: 2.067 ± 1.345
2.067AlaSer: 2.067 ± 1.21
2.067AlaThr: 2.067 ± 1.21
6.718AlaVal: 6.718 ± 2.397
1.034AlaTrp: 1.034 ± 0.784
2.067AlaTyr: 2.067 ± 0.874
0.0AlaXaa: 0.0 ± 0.0
Cys
1.55CysAla: 1.55 ± 0.714
0.0CysCys: 0.0 ± 0.0
1.55CysAsp: 1.55 ± 0.701
0.517CysGlu: 0.517 ± 0.398
2.067CysPhe: 2.067 ± 0.861
1.55CysGly: 1.55 ± 0.977
0.0CysHis: 0.0 ± 0.0
1.55CysIle: 1.55 ± 0.774
1.034CysLys: 1.034 ± 0.63
0.517CysLeu: 0.517 ± 0.622
0.517CysMet: 0.517 ± 0.398
0.0CysAsn: 0.0 ± 0.0
1.55CysPro: 1.55 ± 1.299
1.034CysGln: 1.034 ± 0.467
0.517CysArg: 0.517 ± 0.398
2.584CysSer: 2.584 ± 0.946
2.067CysThr: 2.067 ± 0.935
1.034CysVal: 1.034 ± 0.502
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
5.168AspAla: 5.168 ± 1.248
0.517AspCys: 0.517 ± 0.398
5.168AspAsp: 5.168 ± 1.393
3.101AspGlu: 3.101 ± 1.321
2.584AspPhe: 2.584 ± 0.762
3.618AspGly: 3.618 ± 1.454
1.034AspHis: 1.034 ± 0.502
5.168AspIle: 5.168 ± 1.867
1.034AspLys: 1.034 ± 0.866
4.134AspLeu: 4.134 ± 1.24
1.55AspMet: 1.55 ± 0.737
1.55AspAsn: 1.55 ± 0.939
5.168AspPro: 5.168 ± 2.581
1.034AspGln: 1.034 ± 0.502
1.55AspArg: 1.55 ± 1.014
2.584AspSer: 2.584 ± 1.247
4.134AspThr: 4.134 ± 1.665
3.618AspVal: 3.618 ± 2.117
1.55AspTrp: 1.55 ± 0.631
3.101AspTyr: 3.101 ± 1.115
0.0AspXaa: 0.0 ± 0.0
Glu
4.651GluAla: 4.651 ± 0.904
2.584GluCys: 2.584 ± 0.972
1.55GluAsp: 1.55 ± 0.774
2.067GluGlu: 2.067 ± 0.927
2.584GluPhe: 2.584 ± 1.057
1.034GluGly: 1.034 ± 0.672
2.067GluHis: 2.067 ± 1.005
1.55GluIle: 1.55 ± 0.84
4.134GluLys: 4.134 ± 0.656
5.168GluLeu: 5.168 ± 2.011
1.034GluMet: 1.034 ± 0.675
2.584GluAsn: 2.584 ± 0.974
5.168GluPro: 5.168 ± 1.697
2.584GluGln: 2.584 ± 1.308
0.0GluArg: 0.0 ± 0.0
3.618GluSer: 3.618 ± 1.424
3.101GluThr: 3.101 ± 1.332
2.584GluVal: 2.584 ± 0.973
0.517GluTrp: 0.517 ± 0.57
1.55GluTyr: 1.55 ± 0.586
0.0GluXaa: 0.0 ± 0.0
Phe
4.651PheAla: 4.651 ± 2.003
0.517PheCys: 0.517 ± 0.398
3.618PheAsp: 3.618 ± 0.919
2.584PheGlu: 2.584 ± 0.998
1.55PhePhe: 1.55 ± 0.696
5.168PheGly: 5.168 ± 1.218
0.517PheHis: 0.517 ± 0.598
2.584PheIle: 2.584 ± 0.715
2.584PheLys: 2.584 ± 0.985
3.618PheLeu: 3.618 ± 1.339
2.067PheMet: 2.067 ± 0.873
1.55PheAsn: 1.55 ± 0.791
1.55PhePro: 1.55 ± 0.775
0.517PheGln: 0.517 ± 0.471
2.584PheArg: 2.584 ± 1.204
4.651PheSer: 4.651 ± 1.541
3.101PheThr: 3.101 ± 0.661
3.101PheVal: 3.101 ± 1.027
1.034PheTrp: 1.034 ± 0.502
2.584PheTyr: 2.584 ± 1.149
0.0PheXaa: 0.0 ± 0.0
Gly
3.101GlyAla: 3.101 ± 0.919
1.034GlyCys: 1.034 ± 0.467
4.134GlyAsp: 4.134 ± 1.241
2.584GlyGlu: 2.584 ± 0.601
3.618GlyPhe: 3.618 ± 1.351
4.134GlyGly: 4.134 ± 1.558
1.55GlyHis: 1.55 ± 1.029
9.302GlyIle: 9.302 ± 2.17
2.584GlyLys: 2.584 ± 1.088
5.168GlyLeu: 5.168 ± 1.861
2.584GlyMet: 2.584 ± 1.05
2.067GlyAsn: 2.067 ± 0.935
0.517GlyPro: 0.517 ± 0.433
1.55GlyGln: 1.55 ± 0.808
2.067GlyArg: 2.067 ± 0.902
5.168GlySer: 5.168 ± 1.441
3.101GlyThr: 3.101 ± 0.676
3.618GlyVal: 3.618 ± 1.825
0.517GlyTrp: 0.517 ± 0.398
3.618GlyTyr: 3.618 ± 1.43
0.0GlyXaa: 0.0 ± 0.0
His
2.584HisAla: 2.584 ± 0.935
0.0HisCys: 0.0 ± 0.0
1.034HisAsp: 1.034 ± 0.915
0.0HisGlu: 0.0 ± 0.0
1.034HisPhe: 1.034 ± 0.795
1.034HisGly: 1.034 ± 0.488
0.0HisHis: 0.0 ± 0.0
0.517HisIle: 0.517 ± 0.471
1.034HisLys: 1.034 ± 0.866
0.517HisLeu: 0.517 ± 0.398
0.517HisMet: 0.517 ± 0.471
0.517HisAsn: 0.517 ± 0.398
0.0HisPro: 0.0 ± 0.0
0.0HisGln: 0.0 ± 0.0
1.034HisArg: 1.034 ± 0.713
0.517HisSer: 0.517 ± 0.57
1.034HisThr: 1.034 ± 0.943
1.034HisVal: 1.034 ± 0.943
0.517HisTrp: 0.517 ± 0.433
2.584HisTyr: 2.584 ± 1.88
0.0HisXaa: 0.0 ± 0.0
Ile
6.202IleAla: 6.202 ± 2.513
3.101IleCys: 3.101 ± 1.005
7.235IleAsp: 7.235 ± 2.96
5.168IleGlu: 5.168 ± 1.384
3.618IlePhe: 3.618 ± 1.47
3.101IleGly: 3.101 ± 1.333
1.034IleHis: 1.034 ± 0.668
5.685IleIle: 5.685 ± 1.704
4.134IleLys: 4.134 ± 1.917
4.651IleLeu: 4.651 ± 1.731
1.034IleMet: 1.034 ± 0.673
3.618IleAsn: 3.618 ± 0.99
5.685IlePro: 5.685 ± 1.751
3.618IleGln: 3.618 ± 1.161
3.101IleArg: 3.101 ± 1.346
6.202IleSer: 6.202 ± 1.951
7.235IleThr: 7.235 ± 1.957
3.618IleVal: 3.618 ± 0.955
1.55IleTrp: 1.55 ± 0.793
4.134IleTyr: 4.134 ± 0.923
0.0IleXaa: 0.0 ± 0.0
Lys
5.168LysAla: 5.168 ± 1.49
2.067LysCys: 2.067 ± 1.21
3.101LysAsp: 3.101 ± 1.066
1.034LysGlu: 1.034 ± 0.675
1.55LysPhe: 1.55 ± 0.563
2.584LysGly: 2.584 ± 1.136
0.517LysHis: 0.517 ± 0.433
4.134LysIle: 4.134 ± 0.888
5.685LysLys: 5.685 ± 1.904
6.202LysLeu: 6.202 ± 2.05
2.067LysMet: 2.067 ± 1.114
4.134LysAsn: 4.134 ± 0.926
3.101LysPro: 3.101 ± 0.969
4.134LysGln: 4.134 ± 1.407
3.101LysArg: 3.101 ± 1.167
3.618LysSer: 3.618 ± 0.727
4.651LysThr: 4.651 ± 0.93
3.618LysVal: 3.618 ± 2.215
0.0LysTrp: 0.0 ± 0.0
2.067LysTyr: 2.067 ± 0.807
0.0LysXaa: 0.0 ± 0.0
Leu
4.134LeuAla: 4.134 ± 2.107
1.55LeuCys: 1.55 ± 0.886
3.101LeuAsp: 3.101 ± 0.951
3.618LeuGlu: 3.618 ± 1.365
1.034LeuPhe: 1.034 ± 0.784
9.819LeuGly: 9.819 ± 1.495
2.067LeuHis: 2.067 ± 1.003
10.336LeuIle: 10.336 ± 3.065
5.168LeuLys: 5.168 ± 1.494
9.819LeuLeu: 9.819 ± 3.736
2.584LeuMet: 2.584 ± 1.466
5.685LeuAsn: 5.685 ± 1.491
4.134LeuPro: 4.134 ± 1.204
2.067LeuGln: 2.067 ± 1.005
2.584LeuArg: 2.584 ± 1.039
6.202LeuSer: 6.202 ± 2.061
4.134LeuThr: 4.134 ± 1.44
3.101LeuVal: 3.101 ± 1.319
1.034LeuTrp: 1.034 ± 0.861
4.134LeuTyr: 4.134 ± 1.042
0.0LeuXaa: 0.0 ± 0.0
Met
3.101MetAla: 3.101 ± 1.599
0.0MetCys: 0.0 ± 0.0
1.034MetAsp: 1.034 ± 1.244
1.034MetGlu: 1.034 ± 0.672
1.034MetPhe: 1.034 ± 0.502
1.034MetGly: 1.034 ± 0.697
0.517MetHis: 0.517 ± 0.471
2.584MetIle: 2.584 ± 1.005
0.517MetLys: 0.517 ± 0.398
3.101MetLeu: 3.101 ± 1.217
0.517MetMet: 0.517 ± 0.643
2.067MetAsn: 2.067 ± 0.812
0.517MetPro: 0.517 ± 0.567
0.0MetGln: 0.0 ± 0.0
2.067MetArg: 2.067 ± 0.879
2.067MetSer: 2.067 ± 1.149
2.584MetThr: 2.584 ± 1.226
2.584MetVal: 2.584 ± 0.704
0.0MetTrp: 0.0 ± 0.0
0.517MetTyr: 0.517 ± 0.6
0.0MetXaa: 0.0 ± 0.0
Asn
1.55AsnAla: 1.55 ± 0.873
0.517AsnCys: 0.517 ± 0.398
1.55AsnAsp: 1.55 ± 0.808
5.168AsnGlu: 5.168 ± 2.27
2.067AsnPhe: 2.067 ± 0.831
2.067AsnGly: 2.067 ± 0.996
0.517AsnHis: 0.517 ± 0.471
3.618AsnIle: 3.618 ± 1.78
5.685AsnLys: 5.685 ± 1.845
4.134AsnLeu: 4.134 ± 1.002
0.0AsnMet: 0.0 ± 0.0
2.067AsnAsn: 2.067 ± 0.6
4.134AsnPro: 4.134 ± 1.756
1.034AsnGln: 1.034 ± 0.502
2.584AsnArg: 2.584 ± 1.002
2.584AsnSer: 2.584 ± 1.131
2.584AsnThr: 2.584 ± 0.602
2.067AsnVal: 2.067 ± 1.048
0.517AsnTrp: 0.517 ± 0.598
1.034AsnTyr: 1.034 ± 0.845
0.0AsnXaa: 0.0 ± 0.0
Pro
2.067ProAla: 2.067 ± 0.713
0.517ProCys: 0.517 ± 0.398
5.685ProAsp: 5.685 ± 2.124
4.651ProGlu: 4.651 ± 2.698
4.134ProPhe: 4.134 ± 0.709
0.0ProGly: 0.0 ± 0.0
1.034ProHis: 1.034 ± 0.866
1.55ProIle: 1.55 ± 1.156
3.618ProLys: 3.618 ± 0.966
4.651ProLeu: 4.651 ± 2.293
2.067ProMet: 2.067 ± 0.977
3.101ProAsn: 3.101 ± 1.283
2.584ProPro: 2.584 ± 0.601
2.584ProGln: 2.584 ± 0.877
2.067ProArg: 2.067 ± 0.973
5.685ProSer: 5.685 ± 2.287
4.651ProThr: 4.651 ± 1.723
2.584ProVal: 2.584 ± 0.623
0.517ProTrp: 0.517 ± 0.433
1.034ProTyr: 1.034 ± 0.985
0.0ProXaa: 0.0 ± 0.0
Gln
1.55GlnAla: 1.55 ± 0.632
1.034GlnCys: 1.034 ± 0.784
2.584GlnAsp: 2.584 ± 0.868
1.034GlnGlu: 1.034 ± 0.795
1.55GlnPhe: 1.55 ± 1.414
2.067GlnGly: 2.067 ± 1.446
0.517GlnHis: 0.517 ± 0.471
2.584GlnIle: 2.584 ± 0.744
1.55GlnLys: 1.55 ± 0.793
4.651GlnLeu: 4.651 ± 1.614
0.0GlnMet: 0.0 ± 0.0
1.55GlnAsn: 1.55 ± 0.601
2.584GlnPro: 2.584 ± 0.883
2.067GlnGln: 2.067 ± 0.92
1.55GlnArg: 1.55 ± 0.752
3.101GlnSer: 3.101 ± 1.779
1.55GlnThr: 1.55 ± 0.774
2.584GlnVal: 2.584 ± 0.774
0.517GlnTrp: 0.517 ± 0.57
1.034GlnTyr: 1.034 ± 0.75
0.0GlnXaa: 0.0 ± 0.0
Arg
2.584ArgAla: 2.584 ± 1.311
1.034ArgCys: 1.034 ± 0.866
0.517ArgAsp: 0.517 ± 0.471
2.067ArgGlu: 2.067 ± 1.124
3.618ArgPhe: 3.618 ± 0.835
1.55ArgGly: 1.55 ± 1.299
0.0ArgHis: 0.0 ± 0.0
6.718ArgIle: 6.718 ± 1.915
3.101ArgLys: 3.101 ± 1.32
4.651ArgLeu: 4.651 ± 1.492
1.55ArgMet: 1.55 ± 0.866
2.067ArgAsn: 2.067 ± 1.479
2.584ArgPro: 2.584 ± 0.749
0.517ArgGln: 0.517 ± 0.433
1.034ArgArg: 1.034 ± 0.866
2.067ArgSer: 2.067 ± 1.11
2.067ArgThr: 2.067 ± 1.541
2.067ArgVal: 2.067 ± 0.812
1.034ArgTrp: 1.034 ± 0.675
1.034ArgTyr: 1.034 ± 0.635
0.0ArgXaa: 0.0 ± 0.0
Ser
5.685SerAla: 5.685 ± 1.62
1.034SerCys: 1.034 ± 0.467
4.134SerAsp: 4.134 ± 2.322
1.034SerGlu: 1.034 ± 0.675
4.651SerPhe: 4.651 ± 1.404
6.718SerGly: 6.718 ± 2.194
1.034SerHis: 1.034 ± 0.502
5.168SerIle: 5.168 ± 1.298
6.202SerLys: 6.202 ± 1.228
4.134SerLeu: 4.134 ± 0.947
4.134SerMet: 4.134 ± 1.586
2.067SerAsn: 2.067 ± 0.927
2.067SerPro: 2.067 ± 1.009
1.55SerGln: 1.55 ± 0.701
4.134SerArg: 4.134 ± 1.44
3.101SerSer: 3.101 ± 0.951
0.517SerThr: 0.517 ± 0.398
3.101SerVal: 3.101 ± 1.46
0.517SerTrp: 0.517 ± 0.433
2.584SerTyr: 2.584 ± 0.791
0.0SerXaa: 0.0 ± 0.0
Thr
3.618ThrAla: 3.618 ± 1.002
2.584ThrCys: 2.584 ± 1.243
3.101ThrAsp: 3.101 ± 1.256
3.101ThrGlu: 3.101 ± 0.761
1.55ThrPhe: 1.55 ± 0.586
5.685ThrGly: 5.685 ± 1.609
0.0ThrHis: 0.0 ± 0.0
3.618ThrIle: 3.618 ± 1.208
5.168ThrLys: 5.168 ± 1.541
2.067ThrLeu: 2.067 ± 0.796
1.034ThrMet: 1.034 ± 0.75
2.067ThrAsn: 2.067 ± 0.868
4.134ThrPro: 4.134 ± 1.273
2.584ThrGln: 2.584 ± 0.774
4.134ThrArg: 4.134 ± 0.834
1.55ThrSer: 1.55 ± 0.752
4.134ThrThr: 4.134 ± 1.09
4.651ThrVal: 4.651 ± 1.179
1.55ThrTrp: 1.55 ± 0.793
3.101ThrTyr: 3.101 ± 1.345
0.0ThrXaa: 0.0 ± 0.0
Val
3.618ValAla: 3.618 ± 1.059
1.034ValCys: 1.034 ± 0.636
3.101ValAsp: 3.101 ± 1.322
5.168ValGlu: 5.168 ± 1.48
4.134ValPhe: 4.134 ± 0.957
2.067ValGly: 2.067 ± 0.636
0.517ValHis: 0.517 ± 0.598
7.235ValIle: 7.235 ± 2.06
2.067ValLys: 2.067 ± 0.811
3.101ValLeu: 3.101 ± 0.952
0.517ValMet: 0.517 ± 0.398
3.618ValAsn: 3.618 ± 1.31
4.134ValPro: 4.134 ± 1.092
2.067ValGln: 2.067 ± 1.109
2.067ValArg: 2.067 ± 0.636
4.651ValSer: 4.651 ± 1.269
3.618ValThr: 3.618 ± 1.803
2.067ValVal: 2.067 ± 0.784
0.517ValTrp: 0.517 ± 0.598
2.584ValTyr: 2.584 ± 1.464
0.0ValXaa: 0.0 ± 0.0
Trp
0.0TrpAla: 0.0 ± 0.0
0.0TrpCys: 0.0 ± 0.0
0.517TrpAsp: 0.517 ± 0.57
0.517TrpGlu: 0.517 ± 0.6
0.0TrpPhe: 0.0 ± 0.0
2.584TrpGly: 2.584 ± 1.006
0.517TrpHis: 0.517 ± 0.57
0.517TrpIle: 0.517 ± 0.398
1.034TrpLys: 1.034 ± 0.488
2.067TrpLeu: 2.067 ± 1.481
0.0TrpMet: 0.0 ± 0.0
1.034TrpAsn: 1.034 ± 0.467
1.034TrpPro: 1.034 ± 0.697
0.0TrpGln: 0.0 ± 0.0
1.034TrpArg: 1.034 ± 0.488
0.0TrpSer: 0.0 ± 0.0
0.517TrpThr: 0.517 ± 0.433
1.55TrpVal: 1.55 ± 1.347
1.034TrpTrp: 1.034 ± 0.672
0.517TrpTyr: 0.517 ± 0.433
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.618TyrAla: 3.618 ± 0.902
0.517TyrCys: 0.517 ± 0.471
2.584TyrAsp: 2.584 ± 0.831
2.067TyrGlu: 2.067 ± 0.6
3.101TyrPhe: 3.101 ± 1.04
2.584TyrGly: 2.584 ± 1.225
1.034TyrHis: 1.034 ± 0.943
3.101TyrIle: 3.101 ± 1.107
1.55TyrLys: 1.55 ± 0.988
4.134TyrLeu: 4.134 ± 1.643
0.0TyrMet: 0.0 ± 0.0
1.55TyrAsn: 1.55 ± 1.017
1.034TyrPro: 1.034 ± 0.795
2.584TyrGln: 2.584 ± 1.34
2.584TyrArg: 2.584 ± 0.793
1.55TyrSer: 1.55 ± 1.414
2.584TyrThr: 2.584 ± 1.367
2.584TyrVal: 2.584 ± 1.453
0.517TyrTrp: 0.517 ± 0.433
2.584TyrTyr: 2.584 ± 1.006
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 11 proteins (1936 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski