Novosphingobium subterraneum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4475 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B8Z671|A0A0B8Z671_9SPHN dTDP-glucose 4 6-dehydratase OS=Novosphingobium subterraneum OX=48936 GN=NJ75_04463 PE=3 SV=1
MM1 pKa = 7.02YY2 pKa = 10.5ASLNIAAAGPAADD15 pKa = 3.37QAAFVAALDD24 pKa = 3.81QAHH27 pKa = 6.32ARR29 pKa = 11.84SLHH32 pKa = 6.32SYY34 pKa = 6.89FTQYY38 pKa = 11.58VLTDD42 pKa = 3.76DD43 pKa = 3.83EE44 pKa = 5.33AGYY47 pKa = 10.33IAVDD51 pKa = 3.3EE52 pKa = 4.62GDD54 pKa = 3.72YY55 pKa = 11.34GALPKK60 pKa = 11.06AMLDD64 pKa = 3.53RR65 pKa = 11.84VIDD68 pKa = 3.93TVPGRR73 pKa = 11.84LSDD76 pKa = 3.66EE77 pKa = 4.4FF78 pKa = 5.17

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B8ZG09|A0A0B8ZG09_9SPHN Uncharacterized protein OS=Novosphingobium subterraneum OX=48936 GN=NJ75_02819 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.45GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4475

0

4475

1417282

31

2778

316.7

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.974 ± 0.058

0.897 ± 0.013

5.728 ± 0.025

5.631 ± 0.037

3.613 ± 0.022

8.834 ± 0.041

2.005 ± 0.019

4.85 ± 0.026

3.243 ± 0.028

9.795 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.513 ± 0.018

2.701 ± 0.029

5.263 ± 0.03

3.235 ± 0.019

7.092 ± 0.036

5.447 ± 0.032

5.258 ± 0.031

7.274 ± 0.031

1.454 ± 0.016

2.193 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski