Amino acid dipepetide frequency for Novosphingobium subterraneum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.718AlaAla: 17.718 ± 0.151	1.131AlaCys: 1.131 ± 0.032	7.138AlaAsp: 7.138 ± 0.08	7.88AlaGlu: 7.88 ± 0.084	4.336AlaPhe: 4.336 ± 0.053	10.795AlaGly: 10.795 ± 0.098	2.31AlaHis: 2.31 ± 0.039	6.452AlaIle: 6.452 ± 0.072	4.275AlaLys: 4.275 ± 0.069	13.562AlaLeu: 13.562 ± 0.125	4.14AlaMet: 4.14 ± 0.061	3.409AlaAsn: 3.409 ± 0.057	6.134AlaPro: 6.134 ± 0.078	4.721AlaGln: 4.721 ± 0.06	9.491AlaArg: 9.491 ± 0.087	7.215AlaSer: 7.215 ± 0.084	6.344AlaThr: 6.344 ± 0.08	8.439AlaVal: 8.439 ± 0.085	1.696AlaTrp: 1.696 ± 0.039	2.553AlaTyr: 2.553 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	1.135CysAla: 1.135 ± 0.031	0.128CysCys: 0.128 ± 0.011	0.587CysAsp: 0.587 ± 0.023	0.485CysGlu: 0.485 ± 0.02	0.294CysPhe: 0.294 ± 0.014	0.955CysGly: 0.955 ± 0.023	0.282CysHis: 0.282 ± 0.02	0.404CysIle: 0.404 ± 0.018	0.224CysLys: 0.224 ± 0.012	0.787CysLeu: 0.787 ± 0.024	0.187CysMet: 0.187 ± 0.012	0.257CysAsn: 0.257 ± 0.015	0.54CysPro: 0.54 ± 0.021	0.236CysGln: 0.236 ± 0.014	0.586CysArg: 0.586 ± 0.024	0.527CysSer: 0.527 ± 0.02	0.446CysThr: 0.446 ± 0.019	0.619CysVal: 0.619 ± 0.02	0.121CysTrp: 0.121 ± 0.009	0.164CysTyr: 0.164 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.08AspAla: 7.08 ± 0.077	0.57AspCys: 0.57 ± 0.024	3.032AspAsp: 3.032 ± 0.052	3.461AspGlu: 3.461 ± 0.057	2.168AspPhe: 2.168 ± 0.043	5.372AspGly: 5.372 ± 0.067	1.317AspHis: 1.317 ± 0.034	2.705AspIle: 2.705 ± 0.049	1.862AspLys: 1.862 ± 0.038	5.78AspLeu: 5.78 ± 0.06	1.374AspMet: 1.374 ± 0.03	1.393AspAsn: 1.393 ± 0.033	3.627AspPro: 3.627 ± 0.053	1.681AspGln: 1.681 ± 0.038	4.331AspArg: 4.331 ± 0.065	2.318AspSer: 2.318 ± 0.046	2.542AspThr: 2.542 ± 0.047	3.987AspVal: 3.987 ± 0.055	1.126AspTrp: 1.126 ± 0.033	1.554AspTyr: 1.554 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	7.961GluAla: 7.961 ± 0.106	0.409GluCys: 0.409 ± 0.017	2.886GluAsp: 2.886 ± 0.045	3.095GluGlu: 3.095 ± 0.061	1.707GluPhe: 1.707 ± 0.037	4.8GluGly: 4.8 ± 0.054	1.168GluHis: 1.168 ± 0.034	3.003GluIle: 3.003 ± 0.046	2.139GluLys: 2.139 ± 0.048	5.241GluLeu: 5.241 ± 0.069	1.484GluMet: 1.484 ± 0.033	1.489GluAsn: 1.489 ± 0.033	2.593GluPro: 2.593 ± 0.043	2.246GluGln: 2.246 ± 0.04	4.796GluArg: 4.796 ± 0.067	2.309GluSer: 2.309 ± 0.046	3.095GluThr: 3.095 ± 0.047	4.011GluVal: 4.011 ± 0.054	0.863GluTrp: 0.863 ± 0.025	1.014GluTyr: 1.014 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.871PheAla: 4.871 ± 0.064	0.392PheCys: 0.392 ± 0.018	2.683PheAsp: 2.683 ± 0.052	2.075PheGlu: 2.075 ± 0.04	1.287PhePhe: 1.287 ± 0.039	3.76PheGly: 3.76 ± 0.06	0.739PheHis: 0.739 ± 0.027	1.479PheIle: 1.479 ± 0.032	0.952PheLys: 0.952 ± 0.023	3.141PheLeu: 3.141 ± 0.056	0.795PheMet: 0.795 ± 0.024	1.092PheAsn: 1.092 ± 0.032	1.561PhePro: 1.561 ± 0.039	0.884PheGln: 0.884 ± 0.026	2.259PheArg: 2.259 ± 0.037	2.1PheSer: 2.1 ± 0.041	2.029PheThr: 2.029 ± 0.039	2.546PheVal: 2.546 ± 0.039	0.524PheTrp: 0.524 ± 0.023	0.963PheTyr: 0.963 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	9.621GlyAla: 9.621 ± 0.096	0.896GlyCys: 0.896 ± 0.027	4.532GlyAsp: 4.532 ± 0.067	4.918GlyGlu: 4.918 ± 0.057	3.694GlyPhe: 3.694 ± 0.055	7.863GlyGly: 7.863 ± 0.105	1.864GlyHis: 1.864 ± 0.041	4.418GlyIle: 4.418 ± 0.064	3.743GlyLys: 3.743 ± 0.051	8.617GlyLeu: 8.617 ± 0.094	2.486GlyMet: 2.486 ± 0.051	2.508GlyAsn: 2.508 ± 0.049	3.704GlyPro: 3.704 ± 0.055	3.172GlyGln: 3.172 ± 0.053	5.964GlyArg: 5.964 ± 0.065	5.168GlySer: 5.168 ± 0.079	5.012GlyThr: 5.012 ± 0.084	6.225GlyVal: 6.225 ± 0.075	1.564GlyTrp: 1.564 ± 0.032	2.369GlyTyr: 2.369 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.419HisAla: 2.419 ± 0.046	0.258HisCys: 0.258 ± 0.015	1.194HisAsp: 1.194 ± 0.03	1.14HisGlu: 1.14 ± 0.027	0.852HisPhe: 0.852 ± 0.024	1.98HisGly: 1.98 ± 0.039	0.565HisHis: 0.565 ± 0.022	0.862HisIle: 0.862 ± 0.026	0.54HisLys: 0.54 ± 0.019	1.882HisLeu: 1.882 ± 0.039	0.514HisMet: 0.514 ± 0.018	0.467HisAsn: 0.467 ± 0.019	1.243HisPro: 1.243 ± 0.031	0.551HisGln: 0.551 ± 0.02	1.453HisArg: 1.453 ± 0.03	0.958HisSer: 0.958 ± 0.027	0.778HisThr: 0.778 ± 0.025	1.512HisVal: 1.512 ± 0.036	0.327HisTrp: 0.327 ± 0.017	0.551HisTyr: 0.551 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	7.568IleAla: 7.568 ± 0.081	0.461IleCys: 0.461 ± 0.02	3.676IleAsp: 3.676 ± 0.047	3.527IleGlu: 3.527 ± 0.057	1.425IlePhe: 1.425 ± 0.034	4.788IleGly: 4.788 ± 0.061	0.842IleHis: 0.842 ± 0.024	1.859IleIle: 1.859 ± 0.039	1.312IleLys: 1.312 ± 0.028	3.627IleLeu: 3.627 ± 0.048	0.896IleMet: 0.896 ± 0.027	1.435IleAsn: 1.435 ± 0.033	2.293IlePro: 2.293 ± 0.042	1.131IleGln: 1.131 ± 0.029	3.116IleArg: 3.116 ± 0.042	2.719IleSer: 2.719 ± 0.046	2.452IleThr: 2.452 ± 0.04	3.826IleVal: 3.826 ± 0.057	0.574IleTrp: 0.574 ± 0.02	0.974IleTyr: 0.974 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	4.568LysAla: 4.568 ± 0.074	0.203LysCys: 0.203 ± 0.01	1.703LysAsp: 1.703 ± 0.042	1.346LysGlu: 1.346 ± 0.035	0.984LysPhe: 0.984 ± 0.027	2.968LysGly: 2.968 ± 0.046	0.607LysHis: 0.607 ± 0.022	1.517LysIle: 1.517 ± 0.039	1.088LysLys: 1.088 ± 0.035	3.345LysLeu: 3.345 ± 0.053	0.788LysMet: 0.788 ± 0.025	0.784LysAsn: 0.784 ± 0.023	2.176LysPro: 2.176 ± 0.046	1.001LysGln: 1.001 ± 0.032	2.266LysArg: 2.266 ± 0.046	1.686LysSer: 1.686 ± 0.036	1.668LysThr: 1.668 ± 0.036	2.669LysVal: 2.669 ± 0.046	0.435LysTrp: 0.435 ± 0.019	0.631LysTyr: 0.631 ± 0.024	0.0LysXaa: 0.0 ± 0.0
Leu	14.154LeuAla: 14.154 ± 0.117	0.84LeuCys: 0.84 ± 0.023	5.894LeuAsp: 5.894 ± 0.075	5.123LeuGlu: 5.123 ± 0.068	3.449LeuPhe: 3.449 ± 0.057	8.537LeuGly: 8.537 ± 0.085	1.818LeuHis: 1.818 ± 0.046	4.426LeuIle: 4.426 ± 0.058	3.212LeuLys: 3.212 ± 0.048	9.07LeuLeu: 9.07 ± 0.104	2.142LeuMet: 2.142 ± 0.04	2.441LeuAsn: 2.441 ± 0.041	5.548LeuPro: 5.548 ± 0.074	2.765LeuGln: 2.765 ± 0.049	6.856LeuArg: 6.856 ± 0.082	5.925LeuSer: 5.925 ± 0.07	5.437LeuThr: 5.437 ± 0.067	7.308LeuVal: 7.308 ± 0.084	1.173LeuTrp: 1.173 ± 0.033	1.833LeuTyr: 1.833 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	3.454MetAla: 3.454 ± 0.05	0.182MetCys: 0.182 ± 0.012	1.199MetAsp: 1.199 ± 0.029	1.155MetGlu: 1.155 ± 0.031	0.714MetPhe: 0.714 ± 0.023	1.947MetGly: 1.947 ± 0.038	0.497MetHis: 0.497 ± 0.016	1.295MetIle: 1.295 ± 0.029	1.001MetLys: 1.001 ± 0.028	2.566MetLeu: 2.566 ± 0.049	0.708MetMet: 0.708 ± 0.023	0.711MetAsn: 0.711 ± 0.024	1.554MetPro: 1.554 ± 0.034	0.81MetGln: 0.81 ± 0.021	1.881MetArg: 1.881 ± 0.04	1.453MetSer: 1.453 ± 0.034	1.744MetThr: 1.744 ± 0.034	1.729MetVal: 1.729 ± 0.037	0.248MetTrp: 0.248 ± 0.014	0.279MetTyr: 0.279 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.559AsnAla: 3.559 ± 0.059	0.269AsnCys: 0.269 ± 0.014	1.448AsnAsp: 1.448 ± 0.037	1.19AsnGlu: 1.19 ± 0.029	1.072AsnPhe: 1.072 ± 0.033	2.664AsnGly: 2.664 ± 0.056	0.5AsnHis: 0.5 ± 0.016	1.28AsnIle: 1.28 ± 0.035	0.689AsnLys: 0.689 ± 0.021	2.664AsnLeu: 2.664 ± 0.051	0.586AsnMet: 0.586 ± 0.022	0.705AsnAsn: 0.705 ± 0.031	1.857AsnPro: 1.857 ± 0.04	0.774AsnGln: 0.774 ± 0.025	1.882AsnArg: 1.882 ± 0.033	1.341AsnSer: 1.341 ± 0.035	1.285AsnThr: 1.285 ± 0.033	2.05AsnVal: 2.05 ± 0.045	0.452AsnTrp: 0.452 ± 0.019	0.74AsnTyr: 0.74 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	6.8ProAla: 6.8 ± 0.081	0.379ProCys: 0.379 ± 0.017	3.612ProAsp: 3.612 ± 0.053	3.724ProGlu: 3.724 ± 0.061	1.921ProPhe: 1.921 ± 0.036	4.723ProGly: 4.723 ± 0.065	1.02ProHis: 1.02 ± 0.03	2.305ProIle: 2.305 ± 0.042	1.642ProLys: 1.642 ± 0.034	4.927ProLeu: 4.927 ± 0.071	1.279ProMet: 1.279 ± 0.033	1.308ProAsn: 1.308 ± 0.034	2.541ProPro: 2.541 ± 0.066	1.863ProGln: 1.863 ± 0.036	2.931ProArg: 2.931 ± 0.05	2.789ProSer: 2.789 ± 0.045	2.606ProThr: 2.606 ± 0.044	4.482ProVal: 4.482 ± 0.063	0.711ProTrp: 0.711 ± 0.024	1.065ProTyr: 1.065 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.315GlnAla: 4.315 ± 0.065	0.272GlnCys: 0.272 ± 0.014	1.523GlnAsp: 1.523 ± 0.032	1.382GlnGlu: 1.382 ± 0.034	1.221GlnPhe: 1.221 ± 0.033	2.601GlnGly: 2.601 ± 0.049	0.625GlnHis: 0.625 ± 0.021	1.78GlnIle: 1.78 ± 0.034	0.996GlnLys: 0.996 ± 0.029	3.124GlnLeu: 3.124 ± 0.05	0.868GlnMet: 0.868 ± 0.025	0.843GlnAsn: 0.843 ± 0.027	1.794GlnPro: 1.794 ± 0.04	1.273GlnGln: 1.273 ± 0.032	2.544GlnArg: 2.544 ± 0.047	1.833GlnSer: 1.833 ± 0.036	1.744GlnThr: 1.744 ± 0.037	2.458GlnVal: 2.458 ± 0.045	0.512GlnTrp: 0.512 ± 0.018	0.648GlnTyr: 0.648 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	8.159ArgAla: 8.159 ± 0.092	0.569ArgCys: 0.569 ± 0.023	3.923ArgAsp: 3.923 ± 0.056	4.315ArgGlu: 4.315 ± 0.059	3.001ArgPhe: 3.001 ± 0.05	4.863ArgGly: 4.863 ± 0.068	1.648ArgHis: 1.648 ± 0.037	3.898ArgIle: 3.898 ± 0.047	2.575ArgLys: 2.575 ± 0.045	7.656ArgLeu: 7.656 ± 0.087	1.886ArgMet: 1.886 ± 0.038	2.029ArgAsn: 2.029 ± 0.037	3.535ArgPro: 3.535 ± 0.05	2.596ArgGln: 2.596 ± 0.051	5.284ArgArg: 5.284 ± 0.082	3.787ArgSer: 3.787 ± 0.058	3.526ArgThr: 3.526 ± 0.052	4.707ArgVal: 4.707 ± 0.059	1.14ArgTrp: 1.14 ± 0.03	1.82ArgTyr: 1.82 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	6.856SerAla: 6.856 ± 0.075	0.499SerCys: 0.499 ± 0.021	3.131SerAsp: 3.131 ± 0.051	2.794SerGlu: 2.794 ± 0.052	2.136SerPhe: 2.136 ± 0.043	5.704SerGly: 5.704 ± 0.089	1.035SerHis: 1.035 ± 0.027	2.591SerIle: 2.591 ± 0.046	1.494SerLys: 1.494 ± 0.029	5.483SerLeu: 5.483 ± 0.072	1.234SerMet: 1.234 ± 0.031	1.53SerAsn: 1.53 ± 0.04	2.879SerPro: 2.879 ± 0.049	1.627SerGln: 1.627 ± 0.037	3.649SerArg: 3.649 ± 0.053	3.074SerSer: 3.074 ± 0.056	2.699SerThr: 2.699 ± 0.052	3.942SerVal: 3.942 ± 0.06	0.793SerTrp: 0.793 ± 0.025	1.323SerTyr: 1.323 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	6.394ThrAla: 6.394 ± 0.081	0.488ThrCys: 0.488 ± 0.02	2.799ThrAsp: 2.799 ± 0.047	2.425ThrGlu: 2.425 ± 0.042	1.952ThrPhe: 1.952 ± 0.037	5.361ThrGly: 5.361 ± 0.083	0.95ThrHis: 0.95 ± 0.028	2.833ThrIle: 2.833 ± 0.055	1.36ThrLys: 1.36 ± 0.033	5.591ThrLeu: 5.591 ± 0.068	1.234ThrMet: 1.234 ± 0.033	1.382ThrAsn: 1.382 ± 0.038	3.229ThrPro: 3.229 ± 0.049	1.501ThrGln: 1.501 ± 0.033	3.382ThrArg: 3.382 ± 0.053	2.831ThrSer: 2.831 ± 0.052	2.724ThrThr: 2.724 ± 0.055	4.238ThrVal: 4.238 ± 0.06	0.732ThrTrp: 0.732 ± 0.024	1.177ThrTyr: 1.177 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	9.153ValAla: 9.153 ± 0.086	0.626ValCys: 0.626 ± 0.021	4.133ValAsp: 4.133 ± 0.066	4.522ValGlu: 4.522 ± 0.064	2.467ValPhe: 2.467 ± 0.044	5.554ValGly: 5.554 ± 0.078	1.363ValHis: 1.363 ± 0.034	3.843ValIle: 3.843 ± 0.061	2.143ValLys: 2.143 ± 0.043	7.004ValLeu: 7.004 ± 0.083	1.731ValMet: 1.731 ± 0.038	2.076ValAsn: 2.076 ± 0.039	4.087ValPro: 4.087 ± 0.059	2.141ValGln: 2.141 ± 0.039	5.121ValArg: 5.121 ± 0.062	4.296ValSer: 4.296 ± 0.057	4.562ValThr: 4.562 ± 0.065	5.601ValVal: 5.601 ± 0.069	0.948ValTrp: 0.948 ± 0.029	1.371ValTyr: 1.371 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.337TrpAla: 1.337 ± 0.031	0.15TrpCys: 0.15 ± 0.01	0.758TrpAsp: 0.758 ± 0.025	0.618TrpGlu: 0.618 ± 0.025	0.588TrpPhe: 0.588 ± 0.019	1.022TrpGly: 1.022 ± 0.031	0.376TrpHis: 0.376 ± 0.019	0.717TrpIle: 0.717 ± 0.024	0.554TrpLys: 0.554 ± 0.02	1.776TrpLeu: 1.776 ± 0.039	0.37TrpMet: 0.37 ± 0.017	0.467TrpAsn: 0.467 ± 0.017	0.711TrpPro: 0.711 ± 0.021	0.696TrpGln: 0.696 ± 0.022	1.272TrpArg: 1.272 ± 0.03	0.907TrpSer: 0.907 ± 0.027	0.817TrpThr: 0.817 ± 0.023	0.847TrpVal: 0.847 ± 0.025	0.246TrpTrp: 0.246 ± 0.015	0.315TrpTyr: 0.315 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.621TyrAla: 2.621 ± 0.04	0.242TyrCys: 0.242 ± 0.014	1.429TyrAsp: 1.429 ± 0.035	1.137TyrGlu: 1.137 ± 0.027	0.861TyrPhe: 0.861 ± 0.025	2.083TyrGly: 2.083 ± 0.041	0.521TyrHis: 0.521 ± 0.02	0.834TyrIle: 0.834 ± 0.024	0.631TyrLys: 0.631 ± 0.024	2.109TyrLeu: 2.109 ± 0.042	0.425TyrMet: 0.425 ± 0.018	0.682TyrAsn: 0.682 ± 0.024	1.023TyrPro: 1.023 ± 0.03	0.686TyrGln: 0.686 ± 0.026	1.851TyrArg: 1.851 ± 0.037	1.238TyrSer: 1.238 ± 0.034	1.072TyrThr: 1.072 ± 0.032	1.547TyrVal: 1.547 ± 0.035	0.357TyrTrp: 0.357 ± 0.017	0.584TyrTyr: 0.584 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4475 proteins (1417282 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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