Fur seal faeces associated circular DNA virus
Average proteome isoelectric point is 7.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4X522|S4X522_9CIRC Capsid protein OS=Fur seal faeces associated circular DNA virus OX=1353241 PE=4 SV=1
MM1 pKa = 8.0 PNPQVKK7 pKa = 8.29 MWALTVKK14 pKa = 10.18 NWDD17 pKa = 4.06 LFDD20 pKa = 4.52 HH21 pKa = 6.73 PEE23 pKa = 3.87 KK24 pKa = 10.98 LEE26 pKa = 4.44 EE27 pKa = 4.07 IGNYY31 pKa = 8.18 QDD33 pKa = 4.91 EE34 pKa = 4.74 DD35 pKa = 3.5 GQYY38 pKa = 10.56 LVRR41 pKa = 11.84 YY42 pKa = 9.4 LSIGKK47 pKa = 8.06 HH48 pKa = 4.55 TGEE51 pKa = 3.85 KK52 pKa = 9.0 TGYY55 pKa = 7.23 QHH57 pKa = 7.03 CHH59 pKa = 6.17 LNLEE63 pKa = 4.44 LEE65 pKa = 4.3 KK66 pKa = 10.16 RR67 pKa = 11.84 QYY69 pKa = 7.13 MTWIKK74 pKa = 10.27 RR75 pKa = 11.84 VLFNIPDD82 pKa = 3.39 MHH84 pKa = 6.71 CEE86 pKa = 3.68 KK87 pKa = 10.63 RR88 pKa = 11.84 QGTRR92 pKa = 11.84 EE93 pKa = 3.83 QCDD96 pKa = 3.29 TYY98 pKa = 10.93 LAKK101 pKa = 10.86 DD102 pKa = 3.54 GEE104 pKa = 4.49 FQVIVNRR111 pKa = 11.84 RR112 pKa = 11.84 RR113 pKa = 11.84 IEE115 pKa = 3.98 RR116 pKa = 11.84 GHH118 pKa = 6.89 RR119 pKa = 11.84 SDD121 pKa = 4.94 LDD123 pKa = 4.26 EE124 pKa = 4.13 IHH126 pKa = 7.6 DD127 pKa = 4.22 MIKK130 pKa = 10.29 EE131 pKa = 4.23 GKK133 pKa = 9.83 DD134 pKa = 3.14 LFDD137 pKa = 3.64 VYY139 pKa = 10.53 EE140 pKa = 4.07 SHH142 pKa = 7.11 FASTVRR148 pKa = 11.84 YY149 pKa = 9.59 SSGLEE154 pKa = 3.92 KK155 pKa = 11.04 YY156 pKa = 9.98 IVLHH160 pKa = 6.06 DD161 pKa = 3.97 SKK163 pKa = 10.76 RR164 pKa = 11.84 ARR166 pKa = 11.84 NSEE169 pKa = 3.89 TTAPQVIVYY178 pKa = 8.16 VGPAGSGKK186 pKa = 9.7 SWHH189 pKa = 6.71 CFNDD193 pKa = 3.01 EE194 pKa = 4.25 DD195 pKa = 4.55 YY196 pKa = 11.2 KK197 pKa = 10.67 EE198 pKa = 4.01 SGYY201 pKa = 10.62 RR202 pKa = 11.84 FPIQMYY208 pKa = 8.87 EE209 pKa = 3.61 KK210 pKa = 10.72 VYY212 pKa = 9.87 FDD214 pKa = 3.53 GYY216 pKa = 11.03 NRR218 pKa = 11.84 EE219 pKa = 3.75 KK220 pKa = 10.47 TIWFDD225 pKa = 3.48 EE226 pKa = 4.52 FNGRR230 pKa = 11.84 SMPFGKK236 pKa = 10.18 FCQLADD242 pKa = 4.28 RR243 pKa = 11.84 FPGIYY248 pKa = 6.94 EE249 pKa = 4.19 TKK251 pKa = 10.05 GGSVLISGLKK261 pKa = 9.94 KK262 pKa = 10.24 ILISTISYY270 pKa = 7.75 PATWWAGSNRR280 pKa = 11.84 FNLDD284 pKa = 2.84 PDD286 pKa = 3.38 QLYY289 pKa = 10.55 RR290 pKa = 11.84 RR291 pKa = 11.84 LTKK294 pKa = 10.12 CYY296 pKa = 9.83 YY297 pKa = 10.09 LGPPRR302 pKa = 11.84 RR303 pKa = 11.84 SSDD306 pKa = 2.91 GSIEE310 pKa = 3.84 YY311 pKa = 10.24 AIPLEE316 pKa = 4.23 FNPKK320 pKa = 9.74 EE321 pKa = 4.02 LLTKK325 pKa = 9.84 EE326 pKa = 3.8 QEE328 pKa = 4.54 EE329 pKa = 4.04 ILKK332 pKa = 10.46 RR333 pKa = 11.84 HH334 pKa = 5.31 VKK336 pKa = 10.35 YY337 pKa = 10.55 PSDD340 pKa = 4.12 YY341 pKa = 11.34 NPDD344 pKa = 3.73 DD345 pKa = 5.5 PILMIRR351 pKa = 11.84 DD352 pKa = 3.59 RR353 pKa = 11.84 LKK355 pKa = 11.12 CLLL358 pKa = 4.11
Molecular weight: 42.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.333
IPC2_protein 6.376
IPC_protein 6.491
Toseland 6.649
ProMoST 6.912
Dawson 6.898
Bjellqvist 6.839
Wikipedia 6.898
Rodwell 6.883
Grimsley 6.766
Solomon 6.912
Lehninger 6.912
Nozaki 7.146
DTASelect 7.278
Thurlkill 7.307
EMBOSS 7.307
Sillero 7.293
Patrickios 4.075
IPC_peptide 6.927
IPC2_peptide 6.942
IPC2.peptide.svr19 6.867
Protein with the highest isoelectric point:
>tr|S4X522|S4X522_9CIRC Capsid protein OS=Fur seal faeces associated circular DNA virus OX=1353241 PE=4 SV=1
MM1 pKa = 7.48 SPALTLIALVRR12 pKa = 11.84 CSLLRR17 pKa = 11.84 FFYY20 pKa = 10.94 VRR22 pKa = 11.84 MVYY25 pKa = 9.84 RR26 pKa = 11.84 RR27 pKa = 11.84 YY28 pKa = 9.53 GKK30 pKa = 9.99 RR31 pKa = 11.84 YY32 pKa = 7.59 SRR34 pKa = 11.84 RR35 pKa = 11.84 SGRR38 pKa = 11.84 SRR40 pKa = 11.84 RR41 pKa = 11.84 KK42 pKa = 9.38 VRR44 pKa = 11.84 MSKK47 pKa = 10.27 SLYY50 pKa = 8.61 KK51 pKa = 9.58 RR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 YY55 pKa = 9.22 RR56 pKa = 11.84 RR57 pKa = 11.84 YY58 pKa = 8.82 RR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 SRR63 pKa = 11.84 KK64 pKa = 9.73 SGGLTVSGNRR74 pKa = 11.84 EE75 pKa = 3.72 SCLEE79 pKa = 3.89 YY80 pKa = 11.05 SSFIPLATEE89 pKa = 4.21 VYY91 pKa = 9.18 GSYY94 pKa = 10.42 VGSLQDD100 pKa = 5.56 GFYY103 pKa = 10.05 QWSINPLPAGVCEE116 pKa = 4.1 MPGDD120 pKa = 3.63 ITYY123 pKa = 9.27 TGQWEE128 pKa = 4.2 AAGTDD133 pKa = 3.6 CFKK136 pKa = 11.02 RR137 pKa = 11.84 GIGKK141 pKa = 9.59 KK142 pKa = 9.9 EE143 pKa = 3.74 EE144 pKa = 3.97 ALMLVDD150 pKa = 5.99 LPICISQLSLRR161 pKa = 11.84 NLVRR165 pKa = 11.84 CDD167 pKa = 3.56 TLFEE171 pKa = 4.42 LCKK174 pKa = 10.12 SYY176 pKa = 10.85 RR177 pKa = 11.84 IVSVSCTFTVPEE189 pKa = 4.37 RR190 pKa = 11.84 TSDD193 pKa = 3.89 GPNHH197 pKa = 6.34 NLYY200 pKa = 11.12 LMWTHH205 pKa = 7.19 LPKK208 pKa = 10.61 CRR210 pKa = 11.84 AADD213 pKa = 3.64 AEE215 pKa = 4.87 SCFGMVCKK223 pKa = 10.2 EE224 pKa = 3.88 VVNIEE229 pKa = 4.24 GQYY232 pKa = 10.22 SPSVSGWNWIANPADD247 pKa = 3.22 IAEE250 pKa = 4.54 ACSCDD255 pKa = 3.69 GIEE258 pKa = 4.24 NGRR261 pKa = 11.84 NGWQRR266 pKa = 11.84 KK267 pKa = 5.3 QLAYY271 pKa = 10.37 NSPVTISWRR280 pKa = 11.84 PRR282 pKa = 11.84 HH283 pKa = 5.58 AKK285 pKa = 10.04 IIASHH290 pKa = 6.11 EE291 pKa = 4.09 NYY293 pKa = 9.52 IDD295 pKa = 3.33 SGYY298 pKa = 10.98 AHH300 pKa = 7.14 NAAQTYY306 pKa = 10.72 NKK308 pKa = 9.58 VLPNFASDD316 pKa = 3.53 LKK318 pKa = 10.36 MSRR321 pKa = 11.84 GYY323 pKa = 10.98 LPTDD327 pKa = 3.07 IDD329 pKa = 3.45 DD330 pKa = 3.93 RR331 pKa = 11.84 ARR333 pKa = 11.84 TEE335 pKa = 3.67 RR336 pKa = 11.84 QYY338 pKa = 11.82 WMGPCIRR345 pKa = 11.84 LIDD348 pKa = 4.0 GDD350 pKa = 3.98 IKK352 pKa = 11.05 ASEE355 pKa = 4.24 PVPEE359 pKa = 4.31 TSALNIFDD367 pKa = 4.0 RR368 pKa = 11.84 YY369 pKa = 9.79 GIRR372 pKa = 11.84 VTTTIKK378 pKa = 10.6 VRR380 pKa = 11.84 FKK382 pKa = 11.34 GMNTSDD388 pKa = 4.61 PIFPEE393 pKa = 4.2 YY394 pKa = 9.94 QAA396 pKa = 4.91
Molecular weight: 45.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.869
IPC2_protein 8.887
IPC_protein 8.858
Toseland 9.107
ProMoST 9.18
Dawson 9.531
Bjellqvist 9.472
Wikipedia 9.75
Rodwell 9.619
Grimsley 9.604
Solomon 9.575
Lehninger 9.516
Nozaki 9.531
DTASelect 9.355
Thurlkill 9.399
EMBOSS 9.633
Sillero 9.589
Patrickios 4.342
IPC_peptide 9.56
IPC2_peptide 8.609
IPC2.peptide.svr19 7.826
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
754
358
396
377.0
43.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.642 ± 1.204
2.785 ± 0.541
5.703 ± 0.834
6.233 ± 1.034
3.581 ± 0.397
6.764 ± 0.039
2.122 ± 0.619
6.101 ± 0.029
5.968 ± 1.025
7.692 ± 0.448
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.387 ± 0.099
4.111 ± 0.131
5.04 ± 0.008
3.05 ± 0.378
8.621 ± 1.067
7.56 ± 1.103
4.642 ± 0.294
4.907 ± 0.467
1.989 ± 0.022
6.101 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here