Campylobacter sp. FMV-PI01
Average proteome isoelectric point is 7.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1625 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6L5WKD9|A0A6L5WKD9_9PROT 1-acyl-sn-glycerol-3-phosphate acyltransferase OS=Campylobacter sp. FMV-PI01 OX=2608384 GN=F1B92_05955 PE=4 SV=1
MM1 pKa = 7.41 SKK3 pKa = 10.84 AMDD6 pKa = 4.13 LADD9 pKa = 5.34 EE10 pKa = 4.87 ISDD13 pKa = 4.26 SLSDD17 pKa = 3.68 YY18 pKa = 11.15 LNDD21 pKa = 3.5 VFADD25 pKa = 3.72 DD26 pKa = 4.59 SLPPFHH32 pKa = 7.6 DD33 pKa = 3.82 NKK35 pKa = 10.0 ATNPNFLDD43 pKa = 4.47 PYY45 pKa = 10.09 LNPFTSLTIYY55 pKa = 10.77 DD56 pKa = 4.56 PLILDD61 pKa = 4.71 LDD63 pKa = 4.17 CDD65 pKa = 4.7 GIEE68 pKa = 4.22 TTTLKK73 pKa = 10.95 DD74 pKa = 3.39 GVYY77 pKa = 10.24 FDD79 pKa = 5.41 HH80 pKa = 7.25 NGG82 pKa = 2.96
Molecular weight: 9.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.567
IPC_protein 3.554
Toseland 3.325
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.605
Rodwell 3.389
Grimsley 3.236
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 4.037
Thurlkill 3.414
EMBOSS 3.605
Sillero 3.694
Patrickios 0.769
IPC_peptide 3.554
IPC2_peptide 3.656
IPC2.peptide.svr19 3.681
Protein with the highest isoelectric point:
>tr|A0A6L5WGJ4|A0A6L5WGJ4_9PROT M23 family metallopeptidase OS=Campylobacter sp. FMV-PI01 OX=2608384 GN=F1B92_03670 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.0 QPHH8 pKa = 5.36 KK9 pKa = 7.98 TPRR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.78 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.07 TKK25 pKa = 10.39 NGRR28 pKa = 11.84 KK29 pKa = 9.3 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1625
0
1625
491741
36
3483
302.6
34.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.727 ± 0.069
1.041 ± 0.026
5.967 ± 0.055
6.317 ± 0.07
6.059 ± 0.076
5.889 ± 0.063
1.379 ± 0.023
9.796 ± 0.074
10.069 ± 0.067
9.674 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.185 ± 0.031
7.16 ± 0.076
2.567 ± 0.032
2.499 ± 0.033
2.933 ± 0.049
6.759 ± 0.05
4.239 ± 0.048
5.234 ± 0.056
0.63 ± 0.017
3.875 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here