Pseudomonas sp. M47T1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5675 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|I4N9F8|I4N9F8_9PSED Pyruvate kinase OS=Pseudomonas sp. M47T1 OX=1179778 GN=PMM47T1_02494 PE=3 SV=1
MM1 pKa = 7.23ATLIGVVSQVIGHH14 pKa = 5.69VFAMGADD21 pKa = 3.62GTRR24 pKa = 11.84RR25 pKa = 11.84EE26 pKa = 4.15VHH28 pKa = 5.55QGDD31 pKa = 3.68RR32 pKa = 11.84LFVGEE37 pKa = 4.4QLDD40 pKa = 3.89TGADD44 pKa = 3.49GAVAVHH50 pKa = 6.41LEE52 pKa = 3.95NGKK55 pKa = 9.12EE56 pKa = 3.87LTLGRR61 pKa = 11.84GSEE64 pKa = 4.35VTLSQQMLGGDD75 pKa = 3.97WAPHH79 pKa = 5.34VATQEE84 pKa = 3.92ATAPTQADD92 pKa = 3.65LTDD95 pKa = 3.48VQKK98 pKa = 10.66IQQAIAAGADD108 pKa = 3.37PTKK111 pKa = 10.6AAEE114 pKa = 4.09ATAAGPAALGTSTQGGAEE132 pKa = 4.25GGGHH136 pKa = 5.93SFVLLTEE143 pKa = 4.19TGGAIAPVLGYY154 pKa = 8.98ATAGFNGVADD164 pKa = 3.8FTVPQVAALTNANTAALVVDD184 pKa = 4.08NGVTLTGLGNGVSVDD199 pKa = 3.81EE200 pKa = 5.15ANLADD205 pKa = 4.15GSNPQTAALTQSSSFTVTAADD226 pKa = 3.77GLSNLVVGGITVVNNGAVVGIGQSITTGLGNTLTITGYY264 pKa = 10.92NPTTGQVSYY273 pKa = 10.66SYY275 pKa = 10.06TLTGPDD281 pKa = 3.51TQPAGGGANTVTEE294 pKa = 4.14NLGVSATDD302 pKa = 3.53SNGTNSSGSFTVTVVDD318 pKa = 5.09DD319 pKa = 4.13VPHH322 pKa = 7.42AINDD326 pKa = 3.83NPTTPATEE334 pKa = 3.99QNLVLTGNVLTNDD347 pKa = 2.84IQGADD352 pKa = 4.77RR353 pKa = 11.84IPITATSGPITAGTYY368 pKa = 8.03TGTYY372 pKa = 6.92GTLVLASDD380 pKa = 4.11GTYY383 pKa = 10.16TYY385 pKa = 9.95TLNAAGQAFNDD396 pKa = 3.51LHH398 pKa = 7.78GGGKK402 pKa = 8.49GTDD405 pKa = 3.11SFTYY409 pKa = 9.54TLNDD413 pKa = 3.62ADD415 pKa = 5.02GDD417 pKa = 3.93TSTAVLTLNINNINDD432 pKa = 3.74AVTITAPDD440 pKa = 3.58VNGVNTTVNEE450 pKa = 3.94ANLANGSSPAAAALTQTGQFTVSAPDD476 pKa = 3.5GLQTLTIGGVSVVTNGVGISSPVSVVTQLGTLTINSYY513 pKa = 10.74NATTGVVSYY522 pKa = 10.85SYY524 pKa = 10.12TLTHH528 pKa = 7.25ADD530 pKa = 3.3THH532 pKa = 6.94PDD534 pKa = 3.18VQGPNTITDD543 pKa = 4.03TFAVVATDD551 pKa = 3.64TDD553 pKa = 3.74GSTASTQLTTTIVDD567 pKa = 4.0DD568 pKa = 4.1VPKK571 pKa = 10.71AVADD575 pKa = 4.01TEE577 pKa = 4.69TVTEE581 pKa = 4.42GVTLQGNVLTNDD593 pKa = 2.95ASGADD598 pKa = 3.59GFGGVVGVKK607 pKa = 10.19AGSDD611 pKa = 3.01TSTAVSGGVGVAIEE625 pKa = 4.31GTYY628 pKa = 9.12GTLTINADD636 pKa = 3.51GSSSYY641 pKa = 10.19HH642 pKa = 6.03ANANITKK649 pKa = 9.94DD650 pKa = 3.45GAVSDD655 pKa = 3.86TFTYY659 pKa = 10.69SVADD663 pKa = 3.57KK664 pKa = 11.05DD665 pKa = 4.19GDD667 pKa = 3.54ISTTTVTVTINDD679 pKa = 3.89AGLKK683 pKa = 8.03ATNVDD688 pKa = 3.19GATVFEE694 pKa = 4.52AALPTGSHH702 pKa = 7.21PDD704 pKa = 3.04STAEE708 pKa = 3.97VGTGTVAGSVTGGVGALTYY727 pKa = 10.67SLQGADD733 pKa = 3.16ANGNVTSQYY742 pKa = 9.29GTLHH746 pKa = 6.71LNTDD750 pKa = 3.18GSYY753 pKa = 9.97TYY755 pKa = 9.64TLNVAPQVPGLGTLDD770 pKa = 3.45TFTLVTTDD778 pKa = 3.11SVGNQVDD785 pKa = 3.75SQVTIKK791 pKa = 10.53IVDD794 pKa = 3.84DD795 pKa = 3.97TPTAVPDD802 pKa = 3.83AVTMAEE808 pKa = 4.09GQIAQGNVLANDD820 pKa = 3.28ISGADD825 pKa = 3.44GFAGVVGVRR834 pKa = 11.84AGSDD838 pKa = 2.72TSTPVSGGVGTVIAGAYY855 pKa = 8.27GQLLIQADD863 pKa = 4.59GSAVYY868 pKa = 9.42HH869 pKa = 6.51ANANSVGEE877 pKa = 4.27AGATDD882 pKa = 3.78TFTYY886 pKa = 10.96SVMDD890 pKa = 3.67KK891 pKa = 10.99DD892 pKa = 4.55GDD894 pKa = 3.61ISTTTVTITINDD906 pKa = 3.71AGLVAKK912 pKa = 10.41NVDD915 pKa = 2.99GVTVFEE921 pKa = 4.74AALDD925 pKa = 3.89TTKK928 pKa = 11.11DD929 pKa = 3.83GNDD932 pKa = 3.34LAAGTSTGSHH942 pKa = 7.1PDD944 pKa = 3.29SPLEE948 pKa = 4.1TASGTVVGSVTGGVGTLTYY967 pKa = 10.45SVYY970 pKa = 10.9GSDD973 pKa = 3.7TNGDD977 pKa = 3.21MKK979 pKa = 10.94GQYY982 pKa = 10.39GVIHH986 pKa = 6.31MNADD990 pKa = 2.99GTYY993 pKa = 9.09TYY995 pKa = 10.53TLVTAPQVAGIGTTEE1010 pKa = 4.1NFVLVTTDD1018 pKa = 2.72SAGNHH1023 pKa = 4.97VVSDD1027 pKa = 3.69LTIKK1031 pKa = 10.19IVNDD1035 pKa = 3.38QPVALADD1042 pKa = 3.78SVTLTEE1048 pKa = 4.3GQVAQGNVLANDD1060 pKa = 3.34VSGADD1065 pKa = 3.41GFAGVVGVKK1074 pKa = 10.21AGSDD1078 pKa = 3.19TTTPVSGGVGSVVVGTYY1095 pKa = 10.87GEE1097 pKa = 4.32LVILADD1103 pKa = 3.98GSSIYY1108 pKa = 9.86HH1109 pKa = 6.54ANANSVGVTGATDD1122 pKa = 3.16VFTYY1126 pKa = 10.7SVADD1130 pKa = 3.55KK1131 pKa = 11.05DD1132 pKa = 4.19GDD1134 pKa = 3.54ISTTTVTININDD1146 pKa = 3.77SGLVAKK1152 pKa = 10.03NVNGVTVFEE1161 pKa = 4.48AALDD1165 pKa = 3.96LVKK1168 pKa = 10.83DD1169 pKa = 4.34GNDD1172 pKa = 3.54LAPGTSVGSHH1182 pKa = 7.15PDD1184 pKa = 3.1WTSEE1188 pKa = 4.12TASGTVAGSVTGGVGALTYY1207 pKa = 10.9SVFGSDD1213 pKa = 3.52ASGDD1217 pKa = 3.26IKK1219 pKa = 10.97GQYY1222 pKa = 9.49GVLHH1226 pKa = 6.16MNADD1230 pKa = 3.28GTYY1233 pKa = 9.17TYY1235 pKa = 10.5TLTTAPQVPGIGTTEE1250 pKa = 4.17SFTLVTTDD1258 pKa = 2.92SVGNHH1263 pKa = 5.22VASTLTVKK1271 pKa = 10.41IVDD1274 pKa = 4.27DD1275 pKa = 4.0QPHH1278 pKa = 6.97AFPDD1282 pKa = 4.33FAHH1285 pKa = 6.46LVEE1288 pKa = 4.8GQTAQGNVLANDD1300 pKa = 3.34VSGADD1305 pKa = 3.41GFAGVVGVKK1314 pKa = 10.47AGIDD1318 pKa = 3.38VSKK1321 pKa = 11.09AVIGGVGTVIQGQYY1335 pKa = 10.93GEE1337 pKa = 5.26LIIAADD1343 pKa = 3.82GTSIYY1348 pKa = 9.66HH1349 pKa = 6.67ANANSVGHH1357 pKa = 6.1FGIDD1361 pKa = 2.97VFTYY1365 pKa = 10.63SVADD1369 pKa = 3.55KK1370 pKa = 11.05DD1371 pKa = 4.19GDD1373 pKa = 3.53ISTTTLTIKK1382 pKa = 10.26IDD1384 pKa = 3.93DD1385 pKa = 4.39SGLKK1389 pKa = 10.05ASNVNGVTVFEE1400 pKa = 4.48AALDD1404 pKa = 3.96LVKK1407 pKa = 10.97DD1408 pKa = 4.28GNDD1411 pKa = 3.14LAAGNSVGSHH1421 pKa = 7.02PDD1423 pKa = 2.97WTSEE1427 pKa = 4.12TASGTVAGSVTGGVGALNYY1446 pKa = 10.29SVYY1449 pKa = 9.92GTDD1452 pKa = 3.2ASGDD1456 pKa = 3.22IKK1458 pKa = 10.95GQYY1461 pKa = 9.49GVLHH1465 pKa = 6.16MNADD1469 pKa = 3.28GTYY1472 pKa = 9.41TYY1474 pKa = 10.03TLTIAPQVAGIGTTEE1489 pKa = 4.04TFAVVTTDD1497 pKa = 3.04SVGNHH1502 pKa = 5.27VASTLTIKK1510 pKa = 10.47IVDD1513 pKa = 4.61DD1514 pKa = 3.73QPQAYY1519 pKa = 8.99PDD1521 pKa = 3.84FAHH1524 pKa = 6.08ITEE1527 pKa = 4.59GQTAQGNVLANDD1539 pKa = 3.34VSGADD1544 pKa = 3.41GFAGVVGVKK1553 pKa = 10.32AGADD1557 pKa = 3.3TSKK1560 pKa = 11.19AVTTGVGTVIQGQYY1574 pKa = 11.14GEE1576 pKa = 4.95LFIAADD1582 pKa = 3.91GTSTYY1587 pKa = 9.9HH1588 pKa = 7.16ANANSVNHH1596 pKa = 6.04VGIDD1600 pKa = 3.3VFTYY1604 pKa = 10.64SVADD1608 pKa = 3.55KK1609 pKa = 11.05DD1610 pKa = 4.19GDD1612 pKa = 3.53ISTTTLTIKK1621 pKa = 10.75VGDD1624 pKa = 3.84SHH1626 pKa = 6.79LTATNVDD1633 pKa = 3.36GVTVFEE1639 pKa = 4.6AALDD1643 pKa = 3.96LVKK1646 pKa = 10.97DD1647 pKa = 4.28GNDD1650 pKa = 3.14LAAGNSVGSHH1660 pKa = 7.02PDD1662 pKa = 2.97WTSEE1666 pKa = 4.12TASGTVAGSVTGGVGALNYY1685 pKa = 10.29SVYY1688 pKa = 9.92GTDD1691 pKa = 3.2ASGDD1695 pKa = 3.22IKK1697 pKa = 10.95GQYY1700 pKa = 9.49GVLHH1704 pKa = 6.16MNADD1708 pKa = 3.28GTYY1711 pKa = 9.17TYY1713 pKa = 10.5TLTTAPQVAGIGTTEE1728 pKa = 4.04TFAVVTTDD1736 pKa = 3.04SVGNHH1741 pKa = 5.59VASQLTIKK1749 pKa = 10.44IVDD1752 pKa = 4.51DD1753 pKa = 3.73QPQAYY1758 pKa = 8.1PDD1760 pKa = 3.59SAHH1763 pKa = 6.62ILEE1766 pKa = 4.8GQLAQGNVLQNDD1778 pKa = 3.38VSGADD1783 pKa = 3.38GFAGVVGVKK1792 pKa = 10.25AGSDD1796 pKa = 3.0TSTNVLGGVGTIIQGQYY1813 pKa = 8.53GQLVIAADD1821 pKa = 3.99GSSYY1825 pKa = 11.2YY1826 pKa = 10.11HH1827 pKa = 7.05ANPNSAAAGDD1837 pKa = 3.84VTDD1840 pKa = 3.6TFVYY1844 pKa = 10.51SVADD1848 pKa = 3.42KK1849 pKa = 11.24DD1850 pKa = 4.16GDD1852 pKa = 3.82VSTTTLTISIGNAGLHH1868 pKa = 5.77IVPGDD1873 pKa = 3.66GAGVTVAEE1881 pKa = 4.7AALDD1885 pKa = 3.98LNQDD1889 pKa = 3.84GKK1891 pKa = 10.87DD1892 pKa = 3.51LAAGTVTGSNPDD1904 pKa = 3.22STAEE1908 pKa = 4.03TGSGSVAGSVAGGVGTITYY1927 pKa = 10.25SIDD1930 pKa = 3.31GASNGLFTTAHH1941 pKa = 6.39GVIQLNADD1949 pKa = 3.37GSYY1952 pKa = 10.15TYY1954 pKa = 10.59TLTSAPKK1961 pKa = 9.5TDD1963 pKa = 3.75PSANNGADD1971 pKa = 3.34TTTDD1975 pKa = 3.04TVTYY1979 pKa = 10.19RR1980 pKa = 11.84ATDD1983 pKa = 3.45SQGNTITNQLTINITDD1999 pKa = 4.32DD2000 pKa = 3.56VPQAGAIDD2008 pKa = 3.71RR2009 pKa = 11.84AVIAPEE2015 pKa = 3.96VNSNVLLVIDD2025 pKa = 4.9ISGSMDD2031 pKa = 3.54DD2032 pKa = 3.76PSGVGKK2038 pKa = 10.83LSRR2041 pKa = 11.84LDD2043 pKa = 3.64LAKK2046 pKa = 10.94VSIDD2050 pKa = 3.71KK2051 pKa = 11.13LLDD2054 pKa = 3.3QYY2056 pKa = 12.06SGMGDD2061 pKa = 3.2VKK2063 pKa = 10.83VQVVTFSNGVSTPSSVWVDD2082 pKa = 3.13VATAKK2087 pKa = 10.69AYY2089 pKa = 9.85IAGLHH2094 pKa = 6.29ANGGTNYY2101 pKa = 10.75DD2102 pKa = 3.82YY2103 pKa = 11.32ALSGAEE2109 pKa = 4.31SAFNTNGKK2117 pKa = 9.31LVGGQNVAYY2126 pKa = 9.53FFSDD2130 pKa = 3.75GNPTLSSTHH2139 pKa = 5.74PTSGGSQSGSEE2150 pKa = 4.23TNPNLGDD2157 pKa = 4.53GIDD2160 pKa = 3.76TTEE2163 pKa = 3.91EE2164 pKa = 4.08KK2165 pKa = 10.76AWTDD2169 pKa = 3.47FLNANDD2175 pKa = 3.42IKK2177 pKa = 11.02AYY2179 pKa = 10.36AIGLGNGVDD2188 pKa = 3.78SQYY2191 pKa = 11.14LNPIAYY2197 pKa = 9.79DD2198 pKa = 3.48GTTGTNTNATVVTNLNNLGSVLSGTVQAVALTGSLLTNGTFGADD2242 pKa = 2.84GGFIKK2247 pKa = 10.63SITIDD2252 pKa = 3.52GVTYY2256 pKa = 9.67TYY2258 pKa = 10.88DD2259 pKa = 3.31PKK2261 pKa = 11.5ANNGEE2266 pKa = 4.18GGMTTAGGTSAGTFDD2281 pKa = 3.59TTTNSISVATSHH2293 pKa = 6.5GSTVVVDD2300 pKa = 4.3MDD2302 pKa = 3.74NGAYY2306 pKa = 8.61TYY2308 pKa = 11.03SSASSGSTATFTDD2321 pKa = 3.15KK2322 pKa = 10.54VTFVLADD2329 pKa = 3.48NDD2331 pKa = 4.07GDD2333 pKa = 4.15LSNAANLDD2341 pKa = 3.32ITVTPNSPPVAVADD2355 pKa = 3.75HH2356 pKa = 6.8VITNILSSTIIVPSEE2371 pKa = 3.4ALAANDD2377 pKa = 3.49TDD2379 pKa = 5.17ANGDD2383 pKa = 4.03PLTVANTGFDD2393 pKa = 3.57TGWAPKK2399 pKa = 10.42GADD2402 pKa = 3.52FTATTVQTIQFNGTSNLSSNQIKK2425 pKa = 9.73TIDD2428 pKa = 3.36RR2429 pKa = 11.84SDD2431 pKa = 3.87FNANTSNMTALLVVSGFLGAVGFGSNANASDD2462 pKa = 4.34SITVHH2467 pKa = 6.34LKK2469 pKa = 10.66AGEE2472 pKa = 4.13SLNLSDD2478 pKa = 5.67ASNSHH2483 pKa = 7.2LNMTWQLGNGSATTLGDD2500 pKa = 3.57GGVLTASTDD2509 pKa = 3.07GDD2511 pKa = 4.0YY2512 pKa = 10.78TIHH2515 pKa = 6.53VSHH2518 pKa = 7.2AANHH2522 pKa = 6.19TGQTAYY2528 pKa = 9.78TLDD2531 pKa = 3.34MSINYY2536 pKa = 9.6AGAANYY2542 pKa = 9.17TPDD2545 pKa = 3.92LPATYY2550 pKa = 9.37TVTDD2554 pKa = 3.42NHH2556 pKa = 6.63GGSDD2560 pKa = 3.3TAAATITYY2568 pKa = 10.04QDD2570 pKa = 3.27GHH2572 pKa = 6.13TLTGTAGDD2580 pKa = 5.47DD2581 pKa = 3.29ILISNDD2587 pKa = 3.22SGSTLLGLAGNDD2599 pKa = 3.52FMIGGAGNDD2608 pKa = 3.75KK2609 pKa = 10.7FDD2611 pKa = 4.1GGTGVNTVSYY2621 pKa = 9.4HH2622 pKa = 5.29AAKK2625 pKa = 10.72GPVTVDD2631 pKa = 3.32LSNTGVQDD2639 pKa = 3.66TVNAGHH2645 pKa = 7.03DD2646 pKa = 3.46QLINIQNLIGSDD2658 pKa = 3.82YY2659 pKa = 11.22GDD2661 pKa = 3.95HH2662 pKa = 6.56LTGNSQANVITGGAGNDD2679 pKa = 3.74VINGGGGNDD2688 pKa = 3.93TIIGGLGDD2696 pKa = 3.6NTLTGGDD2703 pKa = 3.54GADD2706 pKa = 3.33TFKK2709 pKa = 11.32YY2710 pKa = 10.12LAGNTGHH2717 pKa = 7.21DD2718 pKa = 4.14TITDD2722 pKa = 4.38FALGTDD2728 pKa = 4.08KK2729 pKa = 11.39LDD2731 pKa = 4.86LSQLLQGDD2739 pKa = 4.35GATASTLDD2747 pKa = 4.1DD2748 pKa = 3.64YY2749 pKa = 12.08LHH2751 pKa = 6.65FKK2753 pKa = 8.75VTGSGSGLVSSIDD2766 pKa = 3.47VSATAGGATTQTIDD2780 pKa = 3.5LQGVNVATHH2789 pKa = 5.56YY2790 pKa = 10.97GITPGAGGTISSHH2803 pKa = 5.95DD2804 pKa = 3.49TATIIGGMLGDD2815 pKa = 4.47HH2816 pKa = 5.92SLKK2819 pKa = 10.58VDD2821 pKa = 3.47VAA2823 pKa = 4.09

Molecular weight:
281.84 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|I4N9M8|I4N9M8_9PSED Tricarboxylate transport protein TctC OS=Pseudomonas sp. M47T1 OX=1179778 GN=PMM47T1_02854 PE=3 SV=1
MM1 pKa = 7.79APRR4 pKa = 11.84TTTPVITFGRR14 pKa = 11.84RR15 pKa = 11.84ATTRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84LITTVLHH28 pKa = 6.23PAAVTGQAGRR38 pKa = 11.84AGTVIAHH45 pKa = 5.9RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84GAARR54 pKa = 11.84VAQAGATGRR63 pKa = 11.84GTATMTTMVALATAGLAAAAAKK85 pKa = 9.92AASKK89 pKa = 11.35ANLL92 pKa = 3.33

Molecular weight:
9.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5675

0

5675

1837506

22

4676

323.8

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.419 ± 0.045

0.988 ± 0.011

5.427 ± 0.024

5.2 ± 0.036

3.585 ± 0.023

8.076 ± 0.033

2.442 ± 0.017

4.594 ± 0.024

3.274 ± 0.029

11.69 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.017

2.992 ± 0.02

4.949 ± 0.024

4.712 ± 0.027

6.288 ± 0.035

5.651 ± 0.028

5.036 ± 0.037

7.334 ± 0.027

1.466 ± 0.014

2.532 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski