Amino acid dipepetide frequency for Pseudomonas sp. M47T1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.389AlaAla: 13.389 ± 0.117
1.297AlaCys: 1.297 ± 0.03
6.215AlaAsp: 6.215 ± 0.063
6.088AlaGlu: 6.088 ± 0.061
3.821AlaPhe: 3.821 ± 0.054
9.102AlaGly: 9.102 ± 0.087
2.478AlaHis: 2.478 ± 0.039
5.202AlaIle: 5.202 ± 0.061
3.586AlaLys: 3.586 ± 0.054
14.299AlaLeu: 14.299 ± 0.118
3.06AlaMet: 3.06 ± 0.045
3.158AlaAsn: 3.158 ± 0.044
5.201AlaPro: 5.201 ± 0.081
5.874AlaGln: 5.874 ± 0.067
7.18AlaArg: 7.18 ± 0.068
6.548AlaSer: 6.548 ± 0.067
5.383AlaThr: 5.383 ± 0.059
8.018AlaVal: 8.018 ± 0.082
1.694AlaTrp: 1.694 ± 0.035
2.599AlaTyr: 2.599 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.18CysAla: 1.18 ± 0.024
0.149CysCys: 0.149 ± 0.01
0.539CysAsp: 0.539 ± 0.017
0.541CysGlu: 0.541 ± 0.017
0.347CysPhe: 0.347 ± 0.014
0.937CysGly: 0.937 ± 0.024
0.276CysHis: 0.276 ± 0.014
0.395CysIle: 0.395 ± 0.014
0.262CysLys: 0.262 ± 0.012
1.14CysLeu: 1.14 ± 0.027
0.23CysMet: 0.23 ± 0.01
0.271CysAsn: 0.271 ± 0.011
0.476CysPro: 0.476 ± 0.018
0.432CysGln: 0.432 ± 0.015
0.591CysArg: 0.591 ± 0.02
0.569CysSer: 0.569 ± 0.018
0.49CysThr: 0.49 ± 0.017
0.675CysVal: 0.675 ± 0.019
0.152CysTrp: 0.152 ± 0.01
0.23CysTyr: 0.23 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
5.999AspAla: 5.999 ± 0.05
0.551AspCys: 0.551 ± 0.016
2.958AspAsp: 2.958 ± 0.05
3.244AspGlu: 3.244 ± 0.04
2.124AspPhe: 2.124 ± 0.038
4.481AspGly: 4.481 ± 0.064
1.298AspHis: 1.298 ± 0.028
2.651AspIle: 2.651 ± 0.038
1.868AspLys: 1.868 ± 0.036
5.946AspLeu: 5.946 ± 0.063
1.178AspMet: 1.178 ± 0.025
1.663AspAsn: 1.663 ± 0.028
2.827AspPro: 2.827 ± 0.039
2.294AspGln: 2.294 ± 0.039
3.109AspArg: 3.109 ± 0.041
2.98AspSer: 2.98 ± 0.041
2.712AspThr: 2.712 ± 0.045
3.691AspVal: 3.691 ± 0.053
0.97AspTrp: 0.97 ± 0.026
1.728AspTyr: 1.728 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
5.904GluAla: 5.904 ± 0.06
0.395GluCys: 0.395 ± 0.014
2.321GluAsp: 2.321 ± 0.043
2.642GluGlu: 2.642 ± 0.056
1.64GluPhe: 1.64 ± 0.035
3.77GluGly: 3.77 ± 0.051
1.742GluHis: 1.742 ± 0.036
2.428GluIle: 2.428 ± 0.04
1.827GluLys: 1.827 ± 0.039
6.262GluLeu: 6.262 ± 0.075
1.197GluMet: 1.197 ± 0.027
1.377GluAsn: 1.377 ± 0.027
2.429GluPro: 2.429 ± 0.047
3.458GluGln: 3.458 ± 0.046
4.319GluArg: 4.319 ± 0.059
2.337GluSer: 2.337 ± 0.039
2.303GluThr: 2.303 ± 0.034
3.848GluVal: 3.848 ± 0.047
0.635GluTrp: 0.635 ± 0.018
1.164GluTyr: 1.164 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
4.1PheAla: 4.1 ± 0.059
0.442PheCys: 0.442 ± 0.016
2.487PheAsp: 2.487 ± 0.042
1.971PheGlu: 1.971 ± 0.035
1.44PhePhe: 1.44 ± 0.034
3.238PheGly: 3.238 ± 0.058
0.781PheHis: 0.781 ± 0.019
1.812PheIle: 1.812 ± 0.036
1.288PheLys: 1.288 ± 0.029
3.099PheLeu: 3.099 ± 0.056
0.884PheMet: 0.884 ± 0.021
1.417PheAsn: 1.417 ± 0.025
1.423PhePro: 1.423 ± 0.03
1.278PheGln: 1.278 ± 0.025
1.65PheArg: 1.65 ± 0.028
2.454PheSer: 2.454 ± 0.039
1.999PheThr: 1.999 ± 0.031
2.528PheVal: 2.528 ± 0.042
0.556PheTrp: 0.556 ± 0.018
1.006PheTyr: 1.006 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
7.761GlyAla: 7.761 ± 0.08
0.955GlyCys: 0.955 ± 0.023
4.028GlyAsp: 4.028 ± 0.049
4.445GlyGlu: 4.445 ± 0.052
3.363GlyPhe: 3.363 ± 0.046
6.206GlyGly: 6.206 ± 0.077
2.046GlyHis: 2.046 ± 0.03
4.074GlyIle: 4.074 ± 0.048
3.313GlyLys: 3.313 ± 0.05
9.388GlyLeu: 9.388 ± 0.088
2.202GlyMet: 2.202 ± 0.043
2.492GlyAsn: 2.492 ± 0.051
2.787GlyPro: 2.787 ± 0.042
3.975GlyGln: 3.975 ± 0.051
4.768GlyArg: 4.768 ± 0.058
4.623GlySer: 4.623 ± 0.061
4.171GlyThr: 4.171 ± 0.088
6.234GlyVal: 6.234 ± 0.068
1.387GlyTrp: 1.387 ± 0.029
2.543GlyTyr: 2.543 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
2.481HisAla: 2.481 ± 0.038
0.315HisCys: 0.315 ± 0.015
1.379HisAsp: 1.379 ± 0.025
1.219HisGlu: 1.219 ± 0.026
1.027HisPhe: 1.027 ± 0.024
2.216HisGly: 2.216 ± 0.038
0.689HisHis: 0.689 ± 0.019
1.081HisIle: 1.081 ± 0.027
0.687HisLys: 0.687 ± 0.019
2.848HisLeu: 2.848 ± 0.044
0.569HisMet: 0.569 ± 0.016
0.667HisAsn: 0.667 ± 0.018
1.437HisPro: 1.437 ± 0.028
1.024HisGln: 1.024 ± 0.027
1.402HisArg: 1.402 ± 0.028
1.364HisSer: 1.364 ± 0.029
1.133HisThr: 1.133 ± 0.021
1.537HisVal: 1.537 ± 0.031
0.536HisTrp: 0.536 ± 0.019
0.813HisTyr: 0.813 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.793IleAla: 5.793 ± 0.068
0.47IleCys: 0.47 ± 0.016
3.263IleAsp: 3.263 ± 0.045
3.025IleGlu: 3.025 ± 0.047
1.381IlePhe: 1.381 ± 0.034
4.398IleGly: 4.398 ± 0.057
0.938IleHis: 0.938 ± 0.024
2.036IleIle: 2.036 ± 0.034
1.67IleLys: 1.67 ± 0.034
3.859IleLeu: 3.859 ± 0.05
0.842IleMet: 0.842 ± 0.021
1.761IleAsn: 1.761 ± 0.033
2.098IlePro: 2.098 ± 0.033
1.566IleGln: 1.566 ± 0.031
2.608IleArg: 2.608 ± 0.036
2.888IleSer: 2.888 ± 0.042
2.546IleThr: 2.546 ± 0.042
3.247IleVal: 3.247 ± 0.045
0.482IleTrp: 0.482 ± 0.016
1.07IleTyr: 1.07 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.219LysAla: 4.219 ± 0.065
0.174LysCys: 0.174 ± 0.009
1.741LysAsp: 1.741 ± 0.033
1.425LysGlu: 1.425 ± 0.036
0.819LysPhe: 0.819 ± 0.022
2.635LysGly: 2.635 ± 0.043
0.786LysHis: 0.786 ± 0.019
1.515LysIle: 1.515 ± 0.032
1.185LysLys: 1.185 ± 0.034
3.484LysLeu: 3.484 ± 0.05
0.715LysMet: 0.715 ± 0.018
0.968LysAsn: 0.968 ± 0.025
2.079LysPro: 2.079 ± 0.037
1.496LysGln: 1.496 ± 0.029
2.282LysArg: 2.282 ± 0.039
1.635LysSer: 1.635 ± 0.03
1.716LysThr: 1.716 ± 0.036
2.853LysVal: 2.853 ± 0.043
0.364LysTrp: 0.364 ± 0.015
0.647LysTyr: 0.647 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
14.726LeuAla: 14.726 ± 0.112
1.214LeuCys: 1.214 ± 0.028
6.672LeuAsp: 6.672 ± 0.071
5.89LeuGlu: 5.89 ± 0.072
4.065LeuPhe: 4.065 ± 0.064
9.488LeuGly: 9.488 ± 0.078
2.68LeuHis: 2.68 ± 0.042
5.282LeuIle: 5.282 ± 0.064
4.195LeuLys: 4.195 ± 0.057
13.072LeuLeu: 13.072 ± 0.136
2.717LeuMet: 2.717 ± 0.045
3.667LeuAsn: 3.667 ± 0.049
6.364LeuPro: 6.364 ± 0.078
4.723LeuGln: 4.723 ± 0.068
7.135LeuArg: 7.135 ± 0.083
7.018LeuSer: 7.018 ± 0.069
5.955LeuThr: 5.955 ± 0.064
8.059LeuVal: 8.059 ± 0.071
1.396LeuTrp: 1.396 ± 0.031
2.588LeuTyr: 2.588 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
2.869MetAla: 2.869 ± 0.042
0.177MetCys: 0.177 ± 0.011
1.09MetAsp: 1.09 ± 0.027
0.937MetGlu: 0.937 ± 0.021
0.693MetPhe: 0.693 ± 0.019
1.828MetGly: 1.828 ± 0.034
0.514MetHis: 0.514 ± 0.016
1.174MetIle: 1.174 ± 0.028
0.883MetLys: 0.883 ± 0.027
2.649MetLeu: 2.649 ± 0.042
0.521MetMet: 0.521 ± 0.018
0.867MetAsn: 0.867 ± 0.018
1.34MetPro: 1.34 ± 0.029
1.011MetGln: 1.011 ± 0.025
1.5MetArg: 1.5 ± 0.03
1.694MetSer: 1.694 ± 0.028
1.484MetThr: 1.484 ± 0.031
1.657MetVal: 1.657 ± 0.03
0.179MetTrp: 0.179 ± 0.01
0.368MetTyr: 0.368 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.302AsnAla: 3.302 ± 0.048
0.273AsnCys: 0.273 ± 0.011
1.63AsnAsp: 1.63 ± 0.034
1.377AsnGlu: 1.377 ± 0.026
1.04AsnPhe: 1.04 ± 0.028
2.638AsnGly: 2.638 ± 0.053
0.676AsnHis: 0.676 ± 0.018
1.374AsnIle: 1.374 ± 0.031
0.886AsnLys: 0.886 ± 0.024
3.527AsnLeu: 3.527 ± 0.051
0.593AsnMet: 0.593 ± 0.018
0.945AsnAsn: 0.945 ± 0.028
1.944AsnPro: 1.944 ± 0.032
1.312AsnGln: 1.312 ± 0.029
1.876AsnArg: 1.876 ± 0.033
1.589AsnSer: 1.589 ± 0.037
1.544AsnThr: 1.544 ± 0.034
2.102AsnVal: 2.102 ± 0.038
0.474AsnTrp: 0.474 ± 0.015
0.821AsnTyr: 0.821 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
6.031ProAla: 6.031 ± 0.073
0.402ProCys: 0.402 ± 0.017
2.933ProAsp: 2.933 ± 0.04
2.894ProGlu: 2.894 ± 0.038
1.849ProPhe: 1.849 ± 0.034
4.165ProGly: 4.165 ± 0.048
1.222ProHis: 1.222 ± 0.024
1.989ProIle: 1.989 ± 0.034
1.534ProLys: 1.534 ± 0.033
5.792ProLeu: 5.792 ± 0.066
1.196ProMet: 1.196 ± 0.027
1.314ProAsn: 1.314 ± 0.031
2.093ProPro: 2.093 ± 0.047
2.41ProGln: 2.41 ± 0.041
2.635ProArg: 2.635 ± 0.044
2.613ProSer: 2.613 ± 0.039
2.412ProThr: 2.412 ± 0.038
3.95ProVal: 3.95 ± 0.052
0.825ProTrp: 0.825 ± 0.021
1.235ProTyr: 1.235 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
6.615GlnAla: 6.615 ± 0.076
0.336GlnCys: 0.336 ± 0.012
1.918GlnAsp: 1.918 ± 0.035
1.824GlnGlu: 1.824 ± 0.032
1.418GlnPhe: 1.418 ± 0.028
3.728GlnGly: 3.728 ± 0.049
1.259GlnHis: 1.259 ± 0.028
1.887GlnIle: 1.887 ± 0.033
1.194GlnLys: 1.194 ± 0.03
5.562GlnLeu: 5.562 ± 0.064
1.115GlnMet: 1.115 ± 0.023
1.049GlnAsn: 1.049 ± 0.024
2.595GlnPro: 2.595 ± 0.043
2.803GlnGln: 2.803 ± 0.057
3.717GlnArg: 3.717 ± 0.059
2.155GlnSer: 2.155 ± 0.035
2.014GlnThr: 2.014 ± 0.031
3.996GlnVal: 3.996 ± 0.057
0.829GlnTrp: 0.829 ± 0.023
1.101GlnTyr: 1.101 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
5.965ArgAla: 5.965 ± 0.064
0.596ArgCys: 0.596 ± 0.019
3.447ArgAsp: 3.447 ± 0.045
3.826ArgGlu: 3.826 ± 0.058
2.589ArgPhe: 2.589 ± 0.042
4.028ArgGly: 4.028 ± 0.057
1.821ArgHis: 1.821 ± 0.033
3.07ArgIle: 3.07 ± 0.039
1.991ArgLys: 1.991 ± 0.037
8.221ArgLeu: 8.221 ± 0.095
1.567ArgMet: 1.567 ± 0.03
1.892ArgAsn: 1.892 ± 0.033
2.798ArgPro: 2.798 ± 0.043
3.461ArgGln: 3.461 ± 0.058
4.146ArgArg: 4.146 ± 0.065
3.23ArgSer: 3.23 ± 0.044
2.811ArgThr: 2.811 ± 0.044
4.42ArgVal: 4.42 ± 0.053
1.055ArgTrp: 1.055 ± 0.028
1.938ArgTyr: 1.938 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.168SerAla: 6.168 ± 0.065
0.432SerCys: 0.432 ± 0.016
2.893SerAsp: 2.893 ± 0.043
2.679SerGlu: 2.679 ± 0.035
2.156SerPhe: 2.156 ± 0.038
5.029SerGly: 5.029 ± 0.059
1.42SerHis: 1.42 ± 0.027
2.701SerIle: 2.701 ± 0.042
1.684SerLys: 1.684 ± 0.033
6.954SerLeu: 6.954 ± 0.073
1.377SerMet: 1.377 ± 0.028
1.684SerAsn: 1.684 ± 0.032
2.74SerPro: 2.74 ± 0.042
2.503SerGln: 2.503 ± 0.041
3.381SerArg: 3.381 ± 0.045
3.297SerSer: 3.297 ± 0.06
3.042SerThr: 3.042 ± 0.051
4.151SerVal: 4.151 ± 0.058
0.781SerTrp: 0.781 ± 0.023
1.441SerTyr: 1.441 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
5.524ThrAla: 5.524 ± 0.062
0.497ThrCys: 0.497 ± 0.015
2.577ThrAsp: 2.577 ± 0.041
2.235ThrGlu: 2.235 ± 0.038
1.9ThrPhe: 1.9 ± 0.035
4.307ThrGly: 4.307 ± 0.069
1.164ThrHis: 1.164 ± 0.028
1.955ThrIle: 1.955 ± 0.04
1.102ThrLys: 1.102 ± 0.029
7.098ThrLeu: 7.098 ± 0.072
0.839ThrMet: 0.839 ± 0.022
1.234ThrAsn: 1.234 ± 0.03
3.265ThrPro: 3.265 ± 0.042
2.244ThrGln: 2.244 ± 0.033
3.11ThrArg: 3.11 ± 0.047
2.745ThrSer: 2.745 ± 0.049
2.653ThrThr: 2.653 ± 0.056
3.856ThrVal: 3.856 ± 0.062
0.756ThrTrp: 0.756 ± 0.023
1.298ThrTyr: 1.298 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
8.3ValAla: 8.3 ± 0.07
0.747ValCys: 0.747 ± 0.021
4.18ValAsp: 4.18 ± 0.042
4.064ValGlu: 4.064 ± 0.056
2.626ValPhe: 2.626 ± 0.039
5.507ValGly: 5.507 ± 0.061
1.582ValHis: 1.582 ± 0.03
3.789ValIle: 3.789 ± 0.043
2.38ValLys: 2.38 ± 0.039
8.363ValLeu: 8.363 ± 0.078
1.812ValMet: 1.812 ± 0.037
2.27ValAsn: 2.27 ± 0.039
3.566ValPro: 3.566 ± 0.047
3.032ValGln: 3.032 ± 0.047
4.359ValArg: 4.359 ± 0.05
4.447ValSer: 4.447 ± 0.061
3.994ValThr: 3.994 ± 0.069
5.755ValVal: 5.755 ± 0.052
0.922ValTrp: 0.922 ± 0.024
1.649ValTyr: 1.649 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.325TrpAla: 1.325 ± 0.03
0.162TrpCys: 0.162 ± 0.01
0.633TrpAsp: 0.633 ± 0.021
0.552TrpGlu: 0.552 ± 0.018
0.57TrpPhe: 0.57 ± 0.019
0.961TrpGly: 0.961 ± 0.028
0.446TrpHis: 0.446 ± 0.016
0.598TrpIle: 0.598 ± 0.018
0.439TrpLys: 0.439 ± 0.014
2.281TrpLeu: 2.281 ± 0.048
0.372TrpMet: 0.372 ± 0.015
0.461TrpAsn: 0.461 ± 0.016
0.7TrpPro: 0.7 ± 0.019
0.953TrpGln: 0.953 ± 0.028
1.117TrpArg: 1.117 ± 0.026
0.801TrpSer: 0.801 ± 0.023
0.641TrpThr: 0.641 ± 0.019
1.047TrpVal: 1.047 ± 0.027
0.251TrpTrp: 0.251 ± 0.013
0.352TrpTyr: 0.352 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.541TyrAla: 2.541 ± 0.041
0.298TyrCys: 0.298 ± 0.013
1.368TyrAsp: 1.368 ± 0.033
1.121TyrGlu: 1.121 ± 0.025
0.982TyrPhe: 0.982 ± 0.023
2.096TyrGly: 2.096 ± 0.039
0.605TyrHis: 0.605 ± 0.018
0.927TyrIle: 0.927 ± 0.022
0.762TyrLys: 0.762 ± 0.024
3.06TyrLeu: 3.06 ± 0.04
0.451TyrMet: 0.451 ± 0.015
0.769TyrAsn: 0.769 ± 0.023
1.332TyrPro: 1.332 ± 0.025
1.269TyrGln: 1.269 ± 0.024
1.99TyrArg: 1.99 ± 0.033
1.525TyrSer: 1.525 ± 0.035
1.356TyrThr: 1.356 ± 0.035
1.72TyrVal: 1.72 ± 0.037
0.419TyrTrp: 0.419 ± 0.016
0.724TyrTyr: 0.724 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5675 proteins (1837506 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski