Marinobacter lutaoensis
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3411 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V2DR87|A0A1V2DR87_9ALTE Uncharacterized protein OS=Marinobacter lutaoensis OX=135739 GN=BTO32_10035 PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 10.14 NPCVTSRR9 pKa = 11.84 WLPLAAVLALAGCGGGSDD27 pKa = 4.16 NEE29 pKa = 4.47 PGLTSDD35 pKa = 3.49 NTGLPEE41 pKa = 4.23 GMVEE45 pKa = 4.09 KK46 pKa = 9.66 TLPAASDD53 pKa = 3.46 EE54 pKa = 4.67 VYY56 pKa = 11.39 VNLEE60 pKa = 3.81 TGEE63 pKa = 4.39 LVDD66 pKa = 6.4 PSDD69 pKa = 3.4 TWHH72 pKa = 6.77 LKK74 pKa = 10.3 ASRR77 pKa = 11.84 LTFNLNGGEE86 pKa = 4.21 SGTGNVGGALAVAQDD101 pKa = 5.17 DD102 pKa = 4.6 FYY104 pKa = 11.68 KK105 pKa = 11.16 DD106 pKa = 3.36 NGDD109 pKa = 3.4 PDD111 pKa = 4.18 EE112 pKa = 4.91 NVFTNASASSEE123 pKa = 4.19 LEE125 pKa = 3.82 HH126 pKa = 8.04 LLGPFEE132 pKa = 6.1 APAAWQTDD140 pKa = 3.8 GLQSAFGSWEE150 pKa = 3.81 TWSSYY155 pKa = 11.06 DD156 pKa = 3.52 YY157 pKa = 10.89 STGQISALSDD167 pKa = 2.48 IGYY170 pKa = 9.5 LVRR173 pKa = 11.84 SAEE176 pKa = 4.28 GNSFARR182 pKa = 11.84 MKK184 pKa = 10.5 VVSFDD189 pKa = 3.24 FPTRR193 pKa = 11.84 ANQGIRR199 pKa = 11.84 DD200 pKa = 3.87 FEE202 pKa = 4.65 FEE204 pKa = 4.47 FSVQGAGEE212 pKa = 4.37 VAFSDD217 pKa = 3.69 QPVTFTPPADD227 pKa = 3.74 YY228 pKa = 11.0 DD229 pKa = 3.85 GGDD232 pKa = 3.12 ACFDD236 pKa = 3.78 FDD238 pKa = 3.66 SGQVVDD244 pKa = 5.52 CDD246 pKa = 4.51 TSDD249 pKa = 2.96 TWDD252 pKa = 3.4 VMVGFSGYY260 pKa = 8.37 NWYY263 pKa = 10.26 LRR265 pKa = 11.84 SNSGPSGSGDD275 pKa = 3.2 GGASGPIGWDD285 pKa = 3.06 EE286 pKa = 4.26 LDD288 pKa = 4.36 SYY290 pKa = 11.05 EE291 pKa = 4.92 ADD293 pKa = 3.36 PGVPQIYY300 pKa = 10.03 AQDD303 pKa = 3.52 STGGVFSEE311 pKa = 4.61 HH312 pKa = 4.68 TWYY315 pKa = 10.52 AYY317 pKa = 11.12 NLLGNHH323 pKa = 7.02 KK324 pKa = 9.35 IWPNFRR330 pKa = 11.84 TYY332 pKa = 10.8 LIKK335 pKa = 10.65 ADD337 pKa = 3.58 IDD339 pKa = 3.63 EE340 pKa = 4.88 TEE342 pKa = 3.96 ASVWALQIVNYY353 pKa = 10.28 YY354 pKa = 10.85 DD355 pKa = 4.51 DD356 pKa = 6.02 DD357 pKa = 4.48 GVSGNPTIRR366 pKa = 11.84 WHH368 pKa = 7.0 RR369 pKa = 11.84 VTLNN373 pKa = 2.99
Molecular weight: 40.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.846
IPC_protein 3.859
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.694
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.215
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.986
Patrickios 1.329
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|A0A1V2DUZ4|A0A1V2DUZ4_9ALTE Poly(Hydroxyalkanoate) granule-associated protein OS=Marinobacter lutaoensis OX=135739 GN=BTO32_07740 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.34 RR12 pKa = 11.84 KK13 pKa = 9.1 RR14 pKa = 11.84 VHH16 pKa = 6.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.91 VLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.44 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3411
0
3411
1153080
15
3247
338.0
37.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.666 ± 0.045
0.932 ± 0.012
5.807 ± 0.035
6.297 ± 0.042
3.553 ± 0.023
7.915 ± 0.041
2.3 ± 0.021
4.728 ± 0.035
2.983 ± 0.035
11.24 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.319 ± 0.019
2.776 ± 0.023
5.118 ± 0.028
3.995 ± 0.027
7.613 ± 0.041
5.259 ± 0.032
5.134 ± 0.03
7.4 ± 0.034
1.413 ± 0.018
2.552 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here