Caulobacter phage Seuss
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 117 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1LM91|A0A0K1LM91_9CAUD Uncharacterized protein OS=Caulobacter phage Seuss OX=1675601 GN=CPT_Seuss116 PE=4 SV=1
MM1 pKa = 7.6 LKK3 pKa = 10.6 LKK5 pKa = 10.58 LYY7 pKa = 10.7 LVTNEE12 pKa = 5.04 DD13 pKa = 3.06 VDD15 pKa = 4.61 GNNLDD20 pKa = 3.6 AFVWAEE26 pKa = 3.95 TLEE29 pKa = 4.17 QAIQLYY35 pKa = 10.65 LPVFDD40 pKa = 5.93 QDD42 pKa = 4.05 TLSDD46 pKa = 3.48 AARR49 pKa = 11.84 VFEE52 pKa = 4.81 VPTTPPPEE60 pKa = 4.07 ARR62 pKa = 11.84 ALEE65 pKa = 4.43 WYY67 pKa = 10.15 DD68 pKa = 3.73 GVIHH72 pKa = 7.05 VYY74 pKa = 10.41 GG75 pKa = 3.82
Molecular weight: 8.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.063
IPC2_protein 4.012
IPC_protein 3.91
Toseland 3.719
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.834
Nozaki 4.024
DTASelect 4.202
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.024
Patrickios 3.083
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.939
Protein with the highest isoelectric point:
>tr|A0A0K1LMV3|A0A0K1LMV3_9CAUD Uncharacterized protein OS=Caulobacter phage Seuss OX=1675601 GN=CPT_Seuss7 PE=4 SV=1
MM1 pKa = 7.2 SQLGYY6 pKa = 10.56 KK7 pKa = 10.68 DD8 pKa = 3.7 MVFDD12 pKa = 3.54 PHH14 pKa = 7.79 AVVWLKK20 pKa = 9.9 PLPKK24 pKa = 9.6 IQPRR28 pKa = 11.84 PMPRR32 pKa = 11.84 HH33 pKa = 4.97 ATALPDD39 pKa = 3.34 HH40 pKa = 6.5 SAGIEE45 pKa = 3.95 FEE47 pKa = 4.88 AFDD50 pKa = 3.66 ARR52 pKa = 11.84 AVHH55 pKa = 6.05 RR56 pKa = 11.84 VFWKK60 pKa = 10.21 FRR62 pKa = 11.84 APNEE66 pKa = 3.67 ALAWRR71 pKa = 11.84 EE72 pKa = 3.53 VDD74 pKa = 4.41 RR75 pKa = 11.84 LCQRR79 pKa = 11.84 AGYY82 pKa = 8.67 NRR84 pKa = 11.84 AWMGLRR90 pKa = 11.84 RR91 pKa = 11.84 AKK93 pKa = 10.16 QEE95 pKa = 3.96 PLVGRR100 pKa = 11.84 DD101 pKa = 3.13 VRR103 pKa = 11.84 EE104 pKa = 3.9 PNEE107 pKa = 3.66 ALRR110 pKa = 11.84 AHH112 pKa = 5.92 VRR114 pKa = 11.84 AALLGRR120 pKa = 11.84 SGPGSAEE127 pKa = 3.5 IGAA130 pKa = 4.33
Molecular weight: 14.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.399
IPC_protein 10.058
Toseland 10.526
ProMoST 10.189
Dawson 10.613
Bjellqvist 10.321
Wikipedia 10.818
Rodwell 10.774
Grimsley 10.657
Solomon 10.745
Lehninger 10.716
Nozaki 10.511
DTASelect 10.306
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.555
Patrickios 10.54
IPC_peptide 10.745
IPC2_peptide 9.194
IPC2.peptide.svr19 8.683
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
117
0
117
26590
55
1822
227.3
25.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.241 ± 0.423
0.786 ± 0.094
6.07 ± 0.168
6.036 ± 0.252
3.768 ± 0.159
6.924 ± 0.185
1.989 ± 0.188
5.322 ± 0.15
4.81 ± 0.191
8.492 ± 0.214
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.689 ± 0.165
3.825 ± 0.188
4.84 ± 0.208
4.31 ± 0.274
6.318 ± 0.221
5.78 ± 0.251
6.657 ± 0.32
6.927 ± 0.247
1.527 ± 0.101
2.689 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here