Amino acid dipepetide frequency for Caulobacter phage Seuss

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.637AlaAla: 12.637 ± 1.464
0.752AlaCys: 0.752 ± 0.202
5.303AlaAsp: 5.303 ± 0.446
6.13AlaGlu: 6.13 ± 0.592
3.159AlaPhe: 3.159 ± 0.386
8.312AlaGly: 8.312 ± 0.534
1.956AlaHis: 1.956 ± 0.296
5.077AlaIle: 5.077 ± 0.44
4.889AlaLys: 4.889 ± 0.406
9.102AlaLeu: 9.102 ± 0.745
3.197AlaMet: 3.197 ± 0.317
3.347AlaAsn: 3.347 ± 0.413
3.385AlaPro: 3.385 ± 0.389
4.739AlaGln: 4.739 ± 0.696
6.544AlaArg: 6.544 ± 0.615
6.356AlaSer: 6.356 ± 0.553
6.206AlaThr: 6.206 ± 0.552
6.657AlaVal: 6.657 ± 0.604
1.918AlaTrp: 1.918 ± 0.306
2.745AlaTyr: 2.745 ± 0.377
0.0AlaXaa: 0.0 ± 0.0
Cys
0.79CysAla: 0.79 ± 0.22
0.263CysCys: 0.263 ± 0.101
0.414CysAsp: 0.414 ± 0.109
0.301CysGlu: 0.301 ± 0.101
0.301CysPhe: 0.301 ± 0.12
0.602CysGly: 0.602 ± 0.176
0.188CysHis: 0.188 ± 0.09
0.301CysIle: 0.301 ± 0.103
0.451CysLys: 0.451 ± 0.117
0.827CysLeu: 0.827 ± 0.188
0.188CysMet: 0.188 ± 0.082
0.301CysAsn: 0.301 ± 0.117
0.489CysPro: 0.489 ± 0.141
0.527CysGln: 0.527 ± 0.149
0.414CysArg: 0.414 ± 0.121
0.263CysSer: 0.263 ± 0.095
0.451CysThr: 0.451 ± 0.134
0.376CysVal: 0.376 ± 0.118
0.188CysTrp: 0.188 ± 0.087
0.226CysTyr: 0.226 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
6.582AspAla: 6.582 ± 0.564
0.564AspCys: 0.564 ± 0.133
3.498AspAsp: 3.498 ± 0.341
3.686AspGlu: 3.686 ± 0.487
2.557AspPhe: 2.557 ± 0.256
4.099AspGly: 4.099 ± 0.429
1.429AspHis: 1.429 ± 0.266
2.708AspIle: 2.708 ± 0.304
2.633AspLys: 2.633 ± 0.34
5.641AspLeu: 5.641 ± 0.506
1.768AspMet: 1.768 ± 0.277
2.332AspAsn: 2.332 ± 0.312
3.686AspPro: 3.686 ± 0.322
3.197AspGln: 3.197 ± 0.372
3.799AspArg: 3.799 ± 0.371
2.069AspSer: 2.069 ± 0.268
3.046AspThr: 3.046 ± 0.372
4.513AspVal: 4.513 ± 0.493
1.241AspTrp: 1.241 ± 0.229
1.655AspTyr: 1.655 ± 0.297
0.0AspXaa: 0.0 ± 0.0
Glu
7.033GluAla: 7.033 ± 0.705
0.263GluCys: 0.263 ± 0.102
2.821GluAsp: 2.821 ± 0.425
3.761GluGlu: 3.761 ± 0.463
2.219GluPhe: 2.219 ± 0.336
3.648GluGly: 3.648 ± 0.481
1.542GluHis: 1.542 ± 0.333
4.175GluIle: 4.175 ± 0.344
2.896GluLys: 2.896 ± 0.368
5.905GluLeu: 5.905 ± 0.5
1.617GluMet: 1.617 ± 0.313
2.332GluAsn: 2.332 ± 0.266
2.934GluPro: 2.934 ± 0.497
2.52GluGln: 2.52 ± 0.319
3.949GluArg: 3.949 ± 0.456
1.88GluSer: 1.88 ± 0.253
3.46GluThr: 3.46 ± 0.393
5.002GluVal: 5.002 ± 0.344
0.752GluTrp: 0.752 ± 0.169
1.655GluTyr: 1.655 ± 0.258
0.0GluXaa: 0.0 ± 0.0
Phe
3.686PheAla: 3.686 ± 0.419
0.451PheCys: 0.451 ± 0.19
2.67PheAsp: 2.67 ± 0.3
3.799PheGlu: 3.799 ± 0.345
1.166PhePhe: 1.166 ± 0.213
2.821PheGly: 2.821 ± 0.306
1.091PheHis: 1.091 ± 0.241
1.617PheIle: 1.617 ± 0.268
2.069PheLys: 2.069 ± 0.271
2.557PheLeu: 2.557 ± 0.306
1.053PheMet: 1.053 ± 0.212
1.429PheAsn: 1.429 ± 0.214
1.429PhePro: 1.429 ± 0.212
1.843PheGln: 1.843 ± 0.252
2.257PheArg: 2.257 ± 0.306
1.692PheSer: 1.692 ± 0.262
2.181PheThr: 2.181 ± 0.253
2.369PheVal: 2.369 ± 0.269
0.489PheTrp: 0.489 ± 0.124
1.015PheTyr: 1.015 ± 0.175
0.0PheXaa: 0.0 ± 0.0
Gly
6.018GlyAla: 6.018 ± 0.512
0.451GlyCys: 0.451 ± 0.114
4.137GlyAsp: 4.137 ± 0.514
4.4GlyGlu: 4.4 ± 0.411
3.234GlyPhe: 3.234 ± 0.308
4.964GlyGly: 4.964 ± 0.319
1.015GlyHis: 1.015 ± 0.215
2.708GlyIle: 2.708 ± 0.363
3.611GlyLys: 3.611 ± 0.346
5.792GlyLeu: 5.792 ± 0.36
2.407GlyMet: 2.407 ± 0.27
3.385GlyAsn: 3.385 ± 0.425
2.294GlyPro: 2.294 ± 0.312
3.159GlyGln: 3.159 ± 0.28
4.325GlyArg: 4.325 ± 0.453
4.325GlySer: 4.325 ± 0.426
4.664GlyThr: 4.664 ± 0.525
5.679GlyVal: 5.679 ± 0.497
0.94GlyTrp: 0.94 ± 0.19
1.73GlyTyr: 1.73 ± 0.267
0.0GlyXaa: 0.0 ± 0.0
His
1.918HisAla: 1.918 ± 0.337
0.226HisCys: 0.226 ± 0.091
1.617HisAsp: 1.617 ± 0.281
0.639HisGlu: 0.639 ± 0.173
0.865HisPhe: 0.865 ± 0.185
1.354HisGly: 1.354 ± 0.289
0.564HisHis: 0.564 ± 0.207
1.128HisIle: 1.128 ± 0.202
0.827HisLys: 0.827 ± 0.197
1.655HisLeu: 1.655 ± 0.233
0.752HisMet: 0.752 ± 0.187
0.602HisAsn: 0.602 ± 0.167
1.429HisPro: 1.429 ± 0.324
1.015HisGln: 1.015 ± 0.232
1.053HisArg: 1.053 ± 0.227
0.865HisSer: 0.865 ± 0.174
1.053HisThr: 1.053 ± 0.227
1.392HisVal: 1.392 ± 0.263
0.564HisTrp: 0.564 ± 0.145
0.376HisTyr: 0.376 ± 0.119
0.0HisXaa: 0.0 ± 0.0
Ile
6.018IleAla: 6.018 ± 0.438
0.414IleCys: 0.414 ± 0.125
4.062IleAsp: 4.062 ± 0.405
3.573IleGlu: 3.573 ± 0.327
1.88IlePhe: 1.88 ± 0.298
3.197IleGly: 3.197 ± 0.366
1.166IleHis: 1.166 ± 0.208
2.181IleIle: 2.181 ± 0.289
2.745IleLys: 2.745 ± 0.285
3.761IleLeu: 3.761 ± 0.493
1.166IleMet: 1.166 ± 0.207
2.257IleAsn: 2.257 ± 0.227
2.294IlePro: 2.294 ± 0.318
2.369IleGln: 2.369 ± 0.269
3.122IleArg: 3.122 ± 0.312
3.122IleSer: 3.122 ± 0.347
3.836IleThr: 3.836 ± 0.463
3.723IleVal: 3.723 ± 0.441
0.903IleTrp: 0.903 ± 0.201
1.429IleTyr: 1.429 ± 0.247
0.0IleXaa: 0.0 ± 0.0
Lys
4.476LysAla: 4.476 ± 0.455
0.301LysCys: 0.301 ± 0.099
2.633LysAsp: 2.633 ± 0.3
2.181LysGlu: 2.181 ± 0.339
1.88LysPhe: 1.88 ± 0.262
2.708LysGly: 2.708 ± 0.375
0.715LysHis: 0.715 ± 0.202
2.783LysIle: 2.783 ± 0.369
1.918LysLys: 1.918 ± 0.245
4.739LysLeu: 4.739 ± 0.462
1.88LysMet: 1.88 ± 0.238
1.542LysAsn: 1.542 ± 0.266
2.971LysPro: 2.971 ± 0.35
1.843LysGln: 1.843 ± 0.268
3.498LysArg: 3.498 ± 0.39
2.971LysSer: 2.971 ± 0.297
3.611LysThr: 3.611 ± 0.454
3.686LysVal: 3.686 ± 0.329
0.564LysTrp: 0.564 ± 0.115
1.204LysTyr: 1.204 ± 0.215
0.0LysXaa: 0.0 ± 0.0
Leu
9.628LeuAla: 9.628 ± 0.75
0.639LeuCys: 0.639 ± 0.151
6.093LeuAsp: 6.093 ± 0.467
4.325LeuGlu: 4.325 ± 0.509
3.159LeuPhe: 3.159 ± 0.331
4.927LeuGly: 4.927 ± 0.468
1.467LeuHis: 1.467 ± 0.249
4.024LeuIle: 4.024 ± 0.451
4.739LeuLys: 4.739 ± 0.561
5.829LeuLeu: 5.829 ± 0.528
2.219LeuMet: 2.219 ± 0.296
3.723LeuAsn: 3.723 ± 0.467
4.024LeuPro: 4.024 ± 0.48
4.024LeuGln: 4.024 ± 0.684
5.19LeuArg: 5.19 ± 0.504
6.055LeuSer: 6.055 ± 0.513
6.206LeuThr: 6.206 ± 0.503
5.829LeuVal: 5.829 ± 0.46
0.715LeuTrp: 0.715 ± 0.167
2.106LeuTyr: 2.106 ± 0.256
0.0LeuXaa: 0.0 ± 0.0
Met
3.234MetAla: 3.234 ± 0.404
0.376MetCys: 0.376 ± 0.135
1.279MetAsp: 1.279 ± 0.222
1.429MetGlu: 1.429 ± 0.235
0.79MetPhe: 0.79 ± 0.222
1.316MetGly: 1.316 ± 0.218
0.564MetHis: 0.564 ± 0.164
1.655MetIle: 1.655 ± 0.225
1.467MetLys: 1.467 ± 0.247
1.918MetLeu: 1.918 ± 0.285
0.376MetMet: 0.376 ± 0.121
1.316MetAsn: 1.316 ± 0.208
1.73MetPro: 1.73 ± 0.267
0.865MetGln: 0.865 ± 0.176
1.73MetArg: 1.73 ± 0.22
2.557MetSer: 2.557 ± 0.342
2.294MetThr: 2.294 ± 0.315
1.467MetVal: 1.467 ± 0.204
0.15MetTrp: 0.15 ± 0.077
0.376MetTyr: 0.376 ± 0.114
0.0MetXaa: 0.0 ± 0.0
Asn
3.385AsnAla: 3.385 ± 0.462
0.226AsnCys: 0.226 ± 0.085
1.467AsnAsp: 1.467 ± 0.244
2.294AsnGlu: 2.294 ± 0.32
1.429AsnPhe: 1.429 ± 0.27
3.31AsnGly: 3.31 ± 0.316
0.715AsnHis: 0.715 ± 0.181
2.557AsnIle: 2.557 ± 0.346
1.015AsnLys: 1.015 ± 0.171
3.385AsnLeu: 3.385 ± 0.382
0.978AsnMet: 0.978 ± 0.189
1.88AsnAsn: 1.88 ± 0.379
2.52AsnPro: 2.52 ± 0.281
1.354AsnGln: 1.354 ± 0.248
2.482AsnArg: 2.482 ± 0.266
2.069AsnSer: 2.069 ± 0.251
2.896AsnThr: 2.896 ± 0.519
2.332AsnVal: 2.332 ± 0.281
0.451AsnTrp: 0.451 ± 0.133
1.504AsnTyr: 1.504 ± 0.232
0.0AsnXaa: 0.0 ± 0.0
Pro
5.077ProAla: 5.077 ± 0.486
0.414ProCys: 0.414 ± 0.153
3.084ProAsp: 3.084 ± 0.347
3.347ProGlu: 3.347 ± 0.352
1.73ProPhe: 1.73 ± 0.284
3.535ProGly: 3.535 ± 0.394
0.903ProHis: 0.903 ± 0.17
2.332ProIle: 2.332 ± 0.305
3.009ProLys: 3.009 ± 0.369
3.611ProLeu: 3.611 ± 0.397
0.865ProMet: 0.865 ± 0.184
1.843ProAsn: 1.843 ± 0.263
2.445ProPro: 2.445 ± 0.331
1.768ProGln: 1.768 ± 0.229
2.369ProArg: 2.369 ± 0.336
3.084ProSer: 3.084 ± 0.36
4.212ProThr: 4.212 ± 0.497
2.821ProVal: 2.821 ± 0.451
0.564ProTrp: 0.564 ± 0.181
1.392ProTyr: 1.392 ± 0.189
0.0ProXaa: 0.0 ± 0.0
Gln
4.964GlnAla: 4.964 ± 0.641
0.263GlnCys: 0.263 ± 0.129
2.257GlnAsp: 2.257 ± 0.33
2.294GlnGlu: 2.294 ± 0.284
1.504GlnPhe: 1.504 ± 0.219
2.557GlnGly: 2.557 ± 0.324
1.015GlnHis: 1.015 ± 0.197
2.821GlnIle: 2.821 ± 0.334
2.144GlnLys: 2.144 ± 0.391
4.701GlnLeu: 4.701 ± 0.628
1.091GlnMet: 1.091 ± 0.237
1.091GlnAsn: 1.091 ± 0.168
2.257GlnPro: 2.257 ± 0.317
1.692GlnGln: 1.692 ± 0.415
3.046GlnArg: 3.046 ± 0.429
2.257GlnSer: 2.257 ± 0.296
2.069GlnThr: 2.069 ± 0.314
3.911GlnVal: 3.911 ± 0.426
0.527GlnTrp: 0.527 ± 0.127
0.602GlnTyr: 0.602 ± 0.145
0.0GlnXaa: 0.0 ± 0.0
Arg
5.641ArgAla: 5.641 ± 0.551
0.527ArgCys: 0.527 ± 0.181
3.987ArgAsp: 3.987 ± 0.32
3.874ArgGlu: 3.874 ± 0.353
2.783ArgPhe: 2.783 ± 0.336
3.498ArgGly: 3.498 ± 0.388
1.392ArgHis: 1.392 ± 0.254
3.422ArgIle: 3.422 ± 0.363
2.708ArgLys: 2.708 ± 0.364
6.544ArgLeu: 6.544 ± 0.53
1.429ArgMet: 1.429 ± 0.238
2.257ArgAsn: 2.257 ± 0.294
2.633ArgPro: 2.633 ± 0.38
3.009ArgGln: 3.009 ± 0.347
4.889ArgArg: 4.889 ± 0.551
3.234ArgSer: 3.234 ± 0.455
3.648ArgThr: 3.648 ± 0.423
4.212ArgVal: 4.212 ± 0.428
1.204ArgTrp: 1.204 ± 0.22
2.294ArgTyr: 2.294 ± 0.275
0.0ArgXaa: 0.0 ± 0.0
Ser
5.453SerAla: 5.453 ± 0.561
0.376SerCys: 0.376 ± 0.143
3.009SerAsp: 3.009 ± 0.334
3.197SerGlu: 3.197 ± 0.387
2.482SerPhe: 2.482 ± 0.359
4.476SerGly: 4.476 ± 0.377
0.527SerHis: 0.527 ± 0.149
4.476SerIle: 4.476 ± 0.478
2.971SerLys: 2.971 ± 0.388
4.476SerLeu: 4.476 ± 0.469
1.843SerMet: 1.843 ± 0.324
2.031SerAsn: 2.031 ± 0.366
3.272SerPro: 3.272 ± 0.376
2.106SerGln: 2.106 ± 0.244
3.347SerArg: 3.347 ± 0.445
3.799SerSer: 3.799 ± 0.527
4.137SerThr: 4.137 ± 0.545
3.31SerVal: 3.31 ± 0.378
0.978SerTrp: 0.978 ± 0.176
1.542SerTyr: 1.542 ± 0.217
0.0SerXaa: 0.0 ± 0.0
Thr
6.243ThrAla: 6.243 ± 0.543
0.527ThrCys: 0.527 ± 0.172
4.062ThrAsp: 4.062 ± 0.395
3.498ThrGlu: 3.498 ± 0.289
2.482ThrPhe: 2.482 ± 0.323
5.829ThrGly: 5.829 ± 0.478
1.392ThrHis: 1.392 ± 0.251
4.099ThrIle: 4.099 ± 0.396
2.67ThrLys: 2.67 ± 0.348
5.641ThrLeu: 5.641 ± 0.457
1.73ThrMet: 1.73 ± 0.367
2.369ThrAsn: 2.369 ± 0.497
3.648ThrPro: 3.648 ± 0.514
2.144ThrGln: 2.144 ± 0.297
3.272ThrArg: 3.272 ± 0.326
4.476ThrSer: 4.476 ± 0.448
4.964ThrThr: 4.964 ± 0.59
4.513ThrVal: 4.513 ± 0.479
0.79ThrTrp: 0.79 ± 0.159
2.219ThrTyr: 2.219 ± 0.33
0.0ThrXaa: 0.0 ± 0.0
Val
6.093ValAla: 6.093 ± 0.478
0.489ValCys: 0.489 ± 0.123
4.889ValAsp: 4.889 ± 0.492
4.964ValGlu: 4.964 ± 0.504
2.557ValPhe: 2.557 ± 0.396
5.641ValGly: 5.641 ± 0.577
1.241ValHis: 1.241 ± 0.236
3.46ValIle: 3.46 ± 0.345
3.535ValLys: 3.535 ± 0.44
5.378ValLeu: 5.378 ± 0.414
1.316ValMet: 1.316 ± 0.21
2.482ValAsn: 2.482 ± 0.353
3.422ValPro: 3.422 ± 0.411
3.159ValGln: 3.159 ± 0.475
4.964ValArg: 4.964 ± 0.394
3.911ValSer: 3.911 ± 0.493
4.739ValThr: 4.739 ± 0.552
4.476ValVal: 4.476 ± 0.509
1.128ValTrp: 1.128 ± 0.221
1.429ValTyr: 1.429 ± 0.279
0.0ValXaa: 0.0 ± 0.0
Trp
1.392TrpAla: 1.392 ± 0.201
0.226TrpCys: 0.226 ± 0.12
1.091TrpAsp: 1.091 ± 0.213
0.715TrpGlu: 0.715 ± 0.178
0.639TrpPhe: 0.639 ± 0.151
0.79TrpGly: 0.79 ± 0.157
0.451TrpHis: 0.451 ± 0.146
0.79TrpIle: 0.79 ± 0.163
0.677TrpLys: 0.677 ± 0.152
1.204TrpLeu: 1.204 ± 0.249
0.489TrpMet: 0.489 ± 0.149
0.602TrpAsn: 0.602 ± 0.149
0.414TrpPro: 0.414 ± 0.141
0.564TrpGln: 0.564 ± 0.149
0.827TrpArg: 0.827 ± 0.224
0.978TrpSer: 0.978 ± 0.202
1.241TrpThr: 1.241 ± 0.187
1.316TrpVal: 1.316 ± 0.238
0.376TrpTrp: 0.376 ± 0.092
0.489TrpTyr: 0.489 ± 0.123
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.144TyrAla: 2.144 ± 0.288
0.113TyrCys: 0.113 ± 0.064
2.332TyrAsp: 2.332 ± 0.249
1.956TyrGlu: 1.956 ± 0.358
0.865TyrPhe: 0.865 ± 0.171
1.655TyrGly: 1.655 ± 0.209
0.564TyrHis: 0.564 ± 0.132
0.903TyrIle: 0.903 ± 0.196
1.128TyrLys: 1.128 ± 0.199
2.257TyrLeu: 2.257 ± 0.306
0.489TyrMet: 0.489 ± 0.129
1.128TyrAsn: 1.128 ± 0.183
1.128TyrPro: 1.128 ± 0.205
1.204TyrGln: 1.204 ± 0.208
2.106TyrArg: 2.106 ± 0.253
1.843TyrSer: 1.843 ± 0.307
1.655TyrThr: 1.655 ± 0.355
1.692TyrVal: 1.692 ± 0.273
0.827TyrTrp: 0.827 ± 0.194
0.903TyrTyr: 0.903 ± 0.194
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 117 proteins (26590 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski