Apis mellifera associated microvirus 18

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UUB4|A0A3S8UUB4_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 18 OX=2494745 PE=4 SV=1
MM1 pKa = 7.18VARR4 pKa = 11.84DD5 pKa = 3.88GDD7 pKa = 3.69PSLRR11 pKa = 11.84EE12 pKa = 4.06DD13 pKa = 4.39LLTMFDD19 pKa = 4.5KK20 pKa = 11.08PFANPRR26 pKa = 11.84LQTSNHH32 pKa = 4.83SFARR36 pKa = 11.84VPQADD41 pKa = 3.5IPRR44 pKa = 11.84STFNRR49 pKa = 11.84SHH51 pKa = 6.84GYY53 pKa = 8.02KK54 pKa = 7.97TTFDD58 pKa = 3.33AGYY61 pKa = 9.42LVPIFVDD68 pKa = 3.68EE69 pKa = 4.73ALPGDD74 pKa = 4.18TFSLHH79 pKa = 4.64MTTFARR85 pKa = 11.84LATPIAPLMDD95 pKa = 4.16NLYY98 pKa = 10.78CDD100 pKa = 3.57VFFFAVPIRR109 pKa = 11.84LVWDD113 pKa = 3.05NWEE116 pKa = 4.44KK117 pKa = 11.05FNGAQDD123 pKa = 4.02NPDD126 pKa = 3.99DD127 pKa = 4.12STDD130 pKa = 3.64FLTPKK135 pKa = 9.55LTTPVGGWATEE146 pKa = 4.0SLSDD150 pKa = 4.5YY151 pKa = 10.44FGLPIGVQHH160 pKa = 6.82ANVHH164 pKa = 5.9AFAHH168 pKa = 6.17RR169 pKa = 11.84AYY171 pKa = 11.1NLIWNEE177 pKa = 3.57WFRR180 pKa = 11.84DD181 pKa = 3.62QNLQDD186 pKa = 3.8SLTVPKK192 pKa = 10.33TDD194 pKa = 4.2GPDD197 pKa = 3.24SATLYY202 pKa = 9.62SLKK205 pKa = 10.4RR206 pKa = 11.84RR207 pKa = 11.84GKK209 pKa = 8.15RR210 pKa = 11.84HH211 pKa = 7.14DD212 pKa = 4.1YY213 pKa = 8.15FTSALPWPQKK223 pKa = 10.69GPAVNLPLGTEE234 pKa = 4.19APITSNFNGAIIGLGEE250 pKa = 4.47ASGQLARR257 pKa = 11.84FRR259 pKa = 11.84PTASTATPAIGAATTSGNPMTASEE283 pKa = 4.06ILEE286 pKa = 4.28WPPSDD291 pKa = 3.41VQYY294 pKa = 11.61YY295 pKa = 10.55NYY297 pKa = 10.91ADD299 pKa = 3.88LSEE302 pKa = 4.4ATAATINQLRR312 pKa = 11.84EE313 pKa = 3.84AFQMQKK319 pKa = 10.54LFEE322 pKa = 5.01RR323 pKa = 11.84DD324 pKa = 3.03ARR326 pKa = 11.84GGTRR330 pKa = 11.84YY331 pKa = 8.58TEE333 pKa = 4.72IIRR336 pKa = 11.84SHH338 pKa = 6.74FGVTSPDD345 pKa = 3.04ARR347 pKa = 11.84LQRR350 pKa = 11.84PEE352 pKa = 3.81YY353 pKa = 10.18LGGGSAPINVAPIPQTSSTDD373 pKa = 2.94ATTPQGNLAAMGTFSHH389 pKa = 6.52SGIGFTKK396 pKa = 10.46SFTEE400 pKa = 3.78HH401 pKa = 7.02CIVIGLISARR411 pKa = 11.84ADD413 pKa = 3.26LNYY416 pKa = 10.22QQGINRR422 pKa = 11.84MWSRR426 pKa = 11.84NTRR429 pKa = 11.84YY430 pKa = 10.59DD431 pKa = 3.87FYY433 pKa = 11.01WPALSHH439 pKa = 6.62IGEE442 pKa = 4.16QAVLNQEE449 pKa = 4.65IYY451 pKa = 10.96AQGTGDD457 pKa = 4.04DD458 pKa = 4.62DD459 pKa = 5.69DD460 pKa = 4.46VFGYY464 pKa = 7.44QEE466 pKa = 4.2RR467 pKa = 11.84YY468 pKa = 9.58AEE470 pKa = 4.04YY471 pKa = 9.71RR472 pKa = 11.84YY473 pKa = 10.38KK474 pKa = 10.77PSLVTGKK481 pKa = 9.86FRR483 pKa = 11.84SDD485 pKa = 2.8ASGSLDD491 pKa = 3.32VWHH494 pKa = 7.26LAQDD498 pKa = 4.08FTGLPVLGDD507 pKa = 3.25TFIQEE512 pKa = 4.55DD513 pKa = 4.42PPVDD517 pKa = 3.27RR518 pKa = 11.84VIAVPSEE525 pKa = 3.67PHH527 pKa = 6.13FLLDD531 pKa = 3.49SAINLKK537 pKa = 9.75CARR540 pKa = 11.84PMPVYY545 pKa = 10.36SVPGLIDD552 pKa = 3.2HH553 pKa = 7.26FF554 pKa = 4.9

Molecular weight: 61.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UU77|A0A3S8UU77_9VIRU Nonstructural protein OS=Apis mellifera associated microvirus 18 OX=2494745 PE=4 SV=1
MM1 pKa = 7.99RR2 pKa = 11.84PPDD5 pKa = 3.76ARR7 pKa = 11.84VLCARR12 pKa = 11.84SHH14 pKa = 6.69RR15 pKa = 11.84SLLSVAMWKK24 pKa = 10.55LIFPVLRR31 pKa = 11.84ASIRR35 pKa = 11.84SIDD38 pKa = 4.31PIVGGALIGGGFNLLSGLFGGSSSLKK64 pKa = 9.63AQKK67 pKa = 10.08AANKK71 pKa = 9.88ANLKK75 pKa = 9.35IAHH78 pKa = 7.02DD79 pKa = 3.4NRR81 pKa = 11.84VFQEE85 pKa = 4.19RR86 pKa = 11.84MSSTAHH92 pKa = 4.67QRR94 pKa = 11.84QVEE97 pKa = 4.25DD98 pKa = 3.34MRR100 pKa = 11.84AAGLNPILSATQGGSSAPQGSSATMVAEE128 pKa = 4.34DD129 pKa = 4.27AMGQAVSDD137 pKa = 3.6AGHH140 pKa = 6.42RR141 pKa = 11.84VASTARR147 pKa = 11.84DD148 pKa = 3.64MFRR151 pKa = 11.84LQTDD155 pKa = 3.47VALADD160 pKa = 3.77AEE162 pKa = 4.39IAGKK166 pKa = 9.9KK167 pKa = 9.23AAALNAASQAEE178 pKa = 4.58LNLATAKK185 pKa = 10.54NVTLQRR191 pKa = 11.84PKK193 pKa = 10.84LIAEE197 pKa = 4.62GRR199 pKa = 11.84ASGHH203 pKa = 6.01SADD206 pKa = 3.75ATIGEE211 pKa = 4.27AAVRR215 pKa = 11.84KK216 pKa = 9.96HH217 pKa = 4.76MSEE220 pKa = 3.89YY221 pKa = 10.63DD222 pKa = 3.66KK223 pKa = 11.62KK224 pKa = 10.6FVPYY228 pKa = 10.06DD229 pKa = 3.54AVSSRR234 pKa = 11.84IFDD237 pKa = 4.99LIGNTGKK244 pKa = 10.57AVGTAIHH251 pKa = 7.34GIRR254 pKa = 11.84NGFSGSKK261 pKa = 8.75NQPRR265 pKa = 11.84STPKK269 pKa = 9.92PPFNPRR275 pKa = 11.84YY276 pKa = 9.54PMGGKK281 pKa = 9.37SGRR284 pKa = 3.59

Molecular weight: 29.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1323

96

554

264.6

29.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.582 ± 1.435

0.907 ± 0.337

6.274 ± 0.596

4.989 ± 0.97

4.837 ± 0.602

6.878 ± 0.793

2.872 ± 0.449

4.157 ± 0.476

4.837 ± 1.253

7.559 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.041 ± 0.388

3.704 ± 0.446

6.803 ± 0.686

3.855 ± 0.205

7.029 ± 0.774

7.483 ± 0.626

5.745 ± 1.016

5.14 ± 0.294

1.134 ± 0.347

3.175 ± 0.672
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski