Streptococcus phage Javan350

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B3I7|A0A4D6B3I7_9CAUD Uncharacterized protein OS=Streptococcus phage Javan350 OX=2548121 GN=Javan350_0042 PE=4 SV=1
MM1 pKa = 7.47SVEE4 pKa = 3.77FDD6 pKa = 4.39EE7 pKa = 5.82IEE9 pKa = 4.13LMQSTGLEE17 pKa = 4.02DD18 pKa = 4.96DD19 pKa = 3.6SGKK22 pKa = 10.85EE23 pKa = 3.6IFEE26 pKa = 4.4GDD28 pKa = 3.93VILWTYY34 pKa = 9.1WDD36 pKa = 3.75EE37 pKa = 4.71FEE39 pKa = 5.82DD40 pKa = 3.65SGRR43 pKa = 11.84AKK45 pKa = 10.21IVFDD49 pKa = 3.35KK50 pKa = 11.53GMFKK54 pKa = 10.89LLDD57 pKa = 3.05IHH59 pKa = 5.96TEE61 pKa = 3.99KK62 pKa = 10.85EE63 pKa = 4.12VWDD66 pKa = 3.96SLFDD70 pKa = 4.84CIEE73 pKa = 3.94NCNVYY78 pKa = 10.4LQGNIYY84 pKa = 9.88EE85 pKa = 4.27NPEE88 pKa = 4.03LLEE91 pKa = 4.58DD92 pKa = 3.89MEE94 pKa = 4.52

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B3F9|A0A4D6B3F9_9CAUD Uncharacterized protein OS=Streptococcus phage Javan350 OX=2548121 GN=Javan350_0012 PE=4 SV=1
MM1 pKa = 7.86KK2 pKa = 10.38YY3 pKa = 11.02NKK5 pKa = 8.8TKK7 pKa = 10.72YY8 pKa = 9.37PNIYY12 pKa = 10.07YY13 pKa = 10.48YY14 pKa = 9.49EE15 pKa = 4.01TAKK18 pKa = 10.52GKK20 pKa = 9.94RR21 pKa = 11.84YY22 pKa = 8.66YY23 pKa = 10.51VRR25 pKa = 11.84RR26 pKa = 11.84SFFFRR31 pKa = 11.84GKK33 pKa = 9.82KK34 pKa = 8.3KK35 pKa = 10.64KK36 pKa = 9.33KK37 pKa = 9.8VKK39 pKa = 10.29VVSQLSLKK47 pKa = 10.36LVQPWW52 pKa = 2.92

Molecular weight: 6.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11923

39

1422

205.6

23.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.601 ± 0.603

0.604 ± 0.116

6.55 ± 0.319

7.624 ± 0.457

4.479 ± 0.331

6.408 ± 0.324

1.518 ± 0.131

6.601 ± 0.265

8.672 ± 0.426

8.63 ± 0.35

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.483 ± 0.173

5.603 ± 0.345

2.734 ± 0.211

3.85 ± 0.247

4.076 ± 0.264

6.659 ± 0.314

5.854 ± 0.528

6.005 ± 0.282

1.283 ± 0.169

3.766 ± 0.352
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski