Odobenus rosmarus divergens (Pacific walrus)
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 29573 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U3ZF50|A0A2U3ZF50_ODORO LOW QUALITY PROTEIN: uncharacterized protein C2orf91 homolog OS=Odobenus rosmarus divergens OX=9708 GN=LOC105757378 PE=4 SV=1
MM1 pKa = 7.22 EE2 pKa = 5.11 AVVLIPCVLGLLLLPLLAGALCVRR26 pKa = 11.84 CRR28 pKa = 11.84 EE29 pKa = 4.25 LPGSYY34 pKa = 8.64 DD35 pKa = 3.36 TTASDD40 pKa = 3.85 SLTPSSIVLKK50 pKa = 10.42 RR51 pKa = 11.84 PPTVVPWPSATSYY64 pKa = 11.6 APVTSYY70 pKa = 11.28 PSLSQPDD77 pKa = 4.54 LLPIPRR83 pKa = 11.84 SPQPHH88 pKa = 6.34 HH89 pKa = 6.86 VPSSRR94 pKa = 11.84 QDD96 pKa = 3.32 SEE98 pKa = 4.34 GANSVASYY106 pKa = 10.76 EE107 pKa = 4.13 NEE109 pKa = 3.86 EE110 pKa = 4.21 PVCEE114 pKa = 4.29 DD115 pKa = 3.85 EE116 pKa = 4.82 EE117 pKa = 4.97 EE118 pKa = 4.14 EE119 pKa = 5.45 DD120 pKa = 3.52 EE121 pKa = 5.4 DD122 pKa = 4.19 YY123 pKa = 11.5 HH124 pKa = 8.35 NEE126 pKa = 4.13 GYY128 pKa = 11.01 LEE130 pKa = 4.22 VLPDD134 pKa = 3.6 STPAPSSAVPPAPVLSNPGLRR155 pKa = 11.84 DD156 pKa = 3.09 SAFSMEE162 pKa = 4.23 SGEE165 pKa = 4.92 DD166 pKa = 3.56 YY167 pKa = 11.72 VNVLEE172 pKa = 4.69 SEE174 pKa = 4.33 EE175 pKa = 4.25 SADD178 pKa = 3.65 VSLDD182 pKa = 3.29 GSRR185 pKa = 11.84 EE186 pKa = 3.96 YY187 pKa = 11.72 VNVSQEE193 pKa = 4.41 LPPMAKK199 pKa = 8.92 TEE201 pKa = 3.92 PAILSSQKK209 pKa = 10.17 VEE211 pKa = 4.63 DD212 pKa = 4.1 EE213 pKa = 4.25 DD214 pKa = 5.59 DD215 pKa = 3.99 EE216 pKa = 4.77 EE217 pKa = 5.11 EE218 pKa = 4.32 EE219 pKa = 4.72 GAPDD223 pKa = 4.17 YY224 pKa = 11.56 EE225 pKa = 4.37 NLL227 pKa = 3.83
Molecular weight: 24.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.617
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.63
Grimsley 3.528
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.012
Thurlkill 3.643
EMBOSS 3.656
Sillero 3.91
Patrickios 0.846
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A2U3VB18|A0A2U3VB18_ODORO mycophenolic acid acyl-glucuronide esterase mitochondrial OS=Odobenus rosmarus divergens OX=9708 GN=ABHD10 PE=4 SV=1
MM1 pKa = 7.35 QSVCVPPLPHH11 pKa = 6.93 LLVGSFYY18 pKa = 10.24 PFPVLPTAQCPVPLPPSSPGSQPSGRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 SRR48 pKa = 11.84 ASPSGHH54 pKa = 6.81 ASQPGPALRR63 pKa = 11.84 SQARR67 pKa = 11.84 ASGPVLRR74 pKa = 11.84 SQARR78 pKa = 11.84 ALGPVLRR85 pKa = 11.84 SQAARR90 pKa = 11.84 PGPALNGRR98 pKa = 11.84 ATPPGTAFRR107 pKa = 11.84 GHH109 pKa = 6.72 ASGLGSALCSRR120 pKa = 11.84 ASAPGPARR128 pKa = 11.84 RR129 pKa = 11.84 RR130 pKa = 11.84 GAPAPGPALRR140 pKa = 11.84 RR141 pKa = 11.84 RR142 pKa = 11.84 HH143 pKa = 5.49 VPVSGPALRR152 pKa = 11.84 SRR154 pKa = 11.84 SRR156 pKa = 11.84 IRR158 pKa = 11.84 GRR160 pKa = 11.84 TSSSGAARR168 pKa = 11.84 RR169 pKa = 11.84 NRR171 pKa = 11.84 GRR173 pKa = 11.84 APAPGPALRR182 pKa = 11.84 SRR184 pKa = 11.84 RR185 pKa = 11.84 RR186 pKa = 11.84 SSSPGAARR194 pKa = 11.84 RR195 pKa = 11.84 SRR197 pKa = 11.84 GRR199 pKa = 11.84 APAPGPALRR208 pKa = 11.84 TLRR211 pKa = 11.84 SRR213 pKa = 11.84 RR214 pKa = 11.84 RR215 pKa = 11.84 SSSPGAARR223 pKa = 11.84 RR224 pKa = 11.84 SRR226 pKa = 11.84 GRR228 pKa = 11.84 APAPGPALRR237 pKa = 11.84 SRR239 pKa = 11.84 RR240 pKa = 11.84 RR241 pKa = 11.84 SSSPGAARR249 pKa = 11.84 RR250 pKa = 11.84 SRR252 pKa = 11.84 GRR254 pKa = 11.84 APAPGPALRR263 pKa = 11.84 SRR265 pKa = 11.84 HH266 pKa = 5.43 SSSPGAASRR275 pKa = 11.84 SRR277 pKa = 11.84 GRR279 pKa = 11.84 APAPGAASRR288 pKa = 11.84 SRR290 pKa = 11.84 RR291 pKa = 11.84 RR292 pKa = 11.84 APASGPARR300 pKa = 11.84 RR301 pKa = 11.84 SRR303 pKa = 11.84 RR304 pKa = 11.84 RR305 pKa = 11.84 SSSPGATRR313 pKa = 11.84 RR314 pKa = 11.84 SRR316 pKa = 11.84 GRR318 pKa = 11.84 ASAPGPALRR327 pKa = 11.84 SRR329 pKa = 11.84 RR330 pKa = 11.84 RR331 pKa = 11.84 SSSPGSARR339 pKa = 11.84 RR340 pKa = 11.84 SRR342 pKa = 11.84 GRR344 pKa = 11.84 VSARR348 pKa = 11.84 GPALRR353 pKa = 11.84 SRR355 pKa = 11.84 RR356 pKa = 11.84 SSSPGPARR364 pKa = 11.84 RR365 pKa = 11.84 SRR367 pKa = 11.84 ASAPGPAYY375 pKa = 10.51 RR376 pKa = 11.84 NRR378 pKa = 11.84 ASSSRR383 pKa = 11.84 ASGPSSALNNQASPPGPALRR403 pKa = 11.84 NRR405 pKa = 11.84 ASGSGPALRR414 pKa = 11.84 NGCPTPGFVLRR425 pKa = 11.84 GRR427 pKa = 11.84 TIQRR431 pKa = 11.84 SFPRR435 pKa = 11.84 SRR437 pKa = 11.84 ASLPVPPGPSPPSSPGVGLRR457 pKa = 11.84 RR458 pKa = 11.84 LVWQSSSSSPNPEE471 pKa = 3.95 VPSLGAQPRR480 pKa = 11.84 WHH482 pKa = 6.76 AVRR485 pKa = 11.84 MRR487 pKa = 11.84 ASSPSPPGRR496 pKa = 11.84 LLPFTEE502 pKa = 5.45 LYY504 pKa = 10.83 GEE506 pKa = 4.47 SSSSSSSTSSSGSPGQSPSSSSSSTSSPKK535 pKa = 10.06 FCGLGSISTPSPASLRR551 pKa = 11.84 RR552 pKa = 11.84 ALLPEE557 pKa = 4.99 LDD559 pKa = 4.03 ALSPLSPGEE568 pKa = 3.88 EE569 pKa = 4.44 DD570 pKa = 3.44 EE571 pKa = 4.5 TGSMPSSPTPPVVV584 pKa = 2.94
Molecular weight: 59.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.979
IPC_protein 12.53
Toseland 12.691
ProMoST 13.188
Dawson 12.691
Bjellqvist 12.691
Wikipedia 13.159
Rodwell 12.193
Grimsley 12.72
Solomon 13.188
Lehninger 13.086
Nozaki 12.691
DTASelect 12.691
Thurlkill 12.691
EMBOSS 13.188
Sillero 12.691
Patrickios 11.901
IPC_peptide 13.188
IPC2_peptide 12.179
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19331
10242
29573
17273837
31
34340
584.1
65.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.073 ± 0.015
2.162 ± 0.013
4.864 ± 0.009
7.116 ± 0.018
3.64 ± 0.01
6.624 ± 0.019
2.568 ± 0.007
4.369 ± 0.012
5.724 ± 0.018
9.843 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.184 ± 0.007
3.622 ± 0.009
6.337 ± 0.021
4.734 ± 0.013
5.738 ± 0.014
8.263 ± 0.017
5.25 ± 0.013
6.022 ± 0.012
1.177 ± 0.005
2.676 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here