Aspergillus uvarum CBS 121591
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12014 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A319CKJ5|A0A319CKJ5_9EURO Uncharacterized protein OS=Aspergillus uvarum CBS 121591 OX=1448315 GN=BO82DRAFT_300919 PE=4 SV=1
MM1 pKa = 7.85 HH2 pKa = 7.35 SFQLLGLAAVGSVVSAAPTASRR24 pKa = 11.84 VSDD27 pKa = 3.71 LVKK30 pKa = 10.58 KK31 pKa = 10.77 SSSTCTFTSASEE43 pKa = 3.88 ASEE46 pKa = 4.57 SISSCSNVVLSNIEE60 pKa = 3.99 VPAGEE65 pKa = 4.33 TLDD68 pKa = 5.08 LSDD71 pKa = 4.59 AADD74 pKa = 3.83 GATITFEE81 pKa = 4.09 GTTSFGYY88 pKa = 9.98 EE89 pKa = 3.81 EE90 pKa = 4.45 WDD92 pKa = 3.63 GPLIRR97 pKa = 11.84 FGGKK101 pKa = 8.74 QLTITQSDD109 pKa = 4.43 GAVIDD114 pKa = 4.31 GDD116 pKa = 4.05 GSRR119 pKa = 11.84 WWDD122 pKa = 3.35 SEE124 pKa = 3.76 GTNGGKK130 pKa = 8.21 TKK132 pKa = 10.35 PKK134 pKa = 9.92 FMYY137 pKa = 10.07 VHH139 pKa = 7.06 DD140 pKa = 4.85 VEE142 pKa = 6.36 DD143 pKa = 3.77 STIKK147 pKa = 10.66 GLQIKK152 pKa = 7.5 NTPVQAISVQATNVYY167 pKa = 8.84 LTDD170 pKa = 3.36 ITIDD174 pKa = 3.67 NSDD177 pKa = 3.3 GDD179 pKa = 4.6 DD180 pKa = 3.68 NGGHH184 pKa = 5.52 NTDD187 pKa = 4.04 GFDD190 pKa = 3.2 ISEE193 pKa = 4.27 STGVYY198 pKa = 9.94 ISGATVKK205 pKa = 10.87 NQDD208 pKa = 3.2 DD209 pKa = 4.45 CIAINSGEE217 pKa = 4.27 NILFTGGTCSGGHH230 pKa = 5.98 GLSIGSVGGRR240 pKa = 11.84 DD241 pKa = 3.68 DD242 pKa = 3.79 NTVKK246 pKa = 10.74 NVTISDD252 pKa = 3.87 STVTDD257 pKa = 3.23 SANGVRR263 pKa = 11.84 IKK265 pKa = 9.9 TIYY268 pKa = 10.64 GDD270 pKa = 3.53 TGDD273 pKa = 3.8 VSEE276 pKa = 4.45 VTYY279 pKa = 11.42 SNIQLSGITDD289 pKa = 3.43 YY290 pKa = 11.75 GIVIEE295 pKa = 4.22 QDD297 pKa = 3.68 YY298 pKa = 11.2 EE299 pKa = 4.24 NGSPTGTPSTGVPITDD315 pKa = 3.4 VTVDD319 pKa = 4.86 GITGSIEE326 pKa = 3.88 DD327 pKa = 4.79 DD328 pKa = 3.32 AVQVYY333 pKa = 9.15 ILCGDD338 pKa = 4.56 GSCSDD343 pKa = 3.75 WTWSGVDD350 pKa = 3.03 ITGGEE355 pKa = 4.17 TSSDD359 pKa = 3.92 CEE361 pKa = 4.18 NVPSGASCC369 pKa = 4.0
Molecular weight: 38.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.77
IPC_protein 3.808
Toseland 3.579
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.478
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.19
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.935
Patrickios 1.888
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A319CWA2|A0A319CWA2_9EURO Uncharacterized protein OS=Aspergillus uvarum CBS 121591 OX=1448315 GN=BO82DRAFT_397350 PE=4 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.84 LAKK15 pKa = 9.55 AQKK18 pKa = 8.59 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.14 WRR45 pKa = 11.84 KK46 pKa = 7.41 TRR48 pKa = 11.84 LGII51 pKa = 4.46
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12014
0
12014
5616582
49
7777
467.5
51.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.921 ± 0.018
1.331 ± 0.009
5.49 ± 0.017
6.001 ± 0.019
3.712 ± 0.013
6.819 ± 0.02
2.54 ± 0.01
4.785 ± 0.014
4.158 ± 0.019
9.311 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.098 ± 0.009
3.402 ± 0.011
6.072 ± 0.026
4.114 ± 0.013
6.271 ± 0.018
8.171 ± 0.028
6.144 ± 0.018
6.297 ± 0.017
1.514 ± 0.009
2.849 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here