Streptacidiphilus bronchialis
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5673 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345T3S0|A0A345T3S0_9ACTN WYL domain-containing protein OS=Streptacidiphilus bronchialis OX=2126346 GN=C7M71_027790 PE=4 SV=1
MM1 pKa = 7.44 SATEE5 pKa = 4.18 PLEE8 pKa = 3.84 VWIDD12 pKa = 3.44 QDD14 pKa = 3.99 LCTGDD19 pKa = 5.01 GICAQYY25 pKa = 10.9 APEE28 pKa = 4.23 VFEE31 pKa = 5.9 LDD33 pKa = 3.05 IDD35 pKa = 3.65 GLAYY39 pKa = 10.7 VKK41 pKa = 10.78 GADD44 pKa = 3.53 EE45 pKa = 4.92 EE46 pKa = 5.33 LRR48 pKa = 11.84 QQPGEE53 pKa = 4.28 SVPVPLTVLQDD64 pKa = 3.69 VVDD67 pKa = 4.16 SAKK70 pKa = 10.25 EE71 pKa = 3.92 CPGDD75 pKa = 4.18 CIHH78 pKa = 6.36 VRR80 pKa = 11.84 RR81 pKa = 11.84 VSDD84 pKa = 3.3 RR85 pKa = 11.84 VEE87 pKa = 4.16 VYY89 pKa = 10.89 GPDD92 pKa = 3.45 AEE94 pKa = 4.31
Molecular weight: 10.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.859
IPC_protein 3.808
Toseland 3.605
ProMoST 3.935
Dawson 3.795
Bjellqvist 3.999
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.101
Thurlkill 3.668
EMBOSS 3.732
Sillero 3.923
Patrickios 1.875
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A345SYE6|A0A345SYE6_9ACTN DNA polymerase III subunit beta OS=Streptacidiphilus bronchialis OX=2126346 GN=C7M71_016310 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILASRR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.69 GRR40 pKa = 11.84 TRR42 pKa = 11.84 LSAA45 pKa = 3.71
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5673
0
5673
1967069
29
7994
346.7
36.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.198 ± 0.057
0.825 ± 0.009
5.82 ± 0.024
5.413 ± 0.034
2.605 ± 0.018
9.651 ± 0.029
2.325 ± 0.016
2.968 ± 0.022
1.735 ± 0.026
10.616 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.605 ± 0.013
1.627 ± 0.02
6.449 ± 0.033
2.906 ± 0.018
8.343 ± 0.037
5.028 ± 0.032
5.981 ± 0.033
8.404 ± 0.03
1.502 ± 0.012
2.001 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here