Cohnella faecalis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Cohnella

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A398CJ32|A0A398CJ32_9BACL Adenylosuccinate lyase OS=Cohnella faecalis OX=2315694 GN=D3H35_16645 PE=3 SV=1
MM1 pKa = 8.27VEE3 pKa = 3.73IAGCSWATEE12 pKa = 3.95LADD15 pKa = 4.58ARR17 pKa = 11.84TEE19 pKa = 4.19EE20 pKa = 4.08ITGCGWATEE29 pKa = 4.19LADD32 pKa = 3.67EE33 pKa = 4.58RR34 pKa = 11.84VVEE37 pKa = 4.35IAGCSWATEE46 pKa = 3.95LADD49 pKa = 4.58ARR51 pKa = 11.84TEE53 pKa = 4.41EE54 pKa = 4.35ITCCGWATEE63 pKa = 4.18LADD66 pKa = 3.94EE67 pKa = 4.32RR68 pKa = 11.84MEE70 pKa = 5.77EE71 pKa = 4.11ITCCGWATEE80 pKa = 4.22LADD83 pKa = 3.67EE84 pKa = 4.58RR85 pKa = 11.84VVEE88 pKa = 4.35IAGCSWATGLADD100 pKa = 3.78VRR102 pKa = 11.84MEE104 pKa = 4.81EE105 pKa = 3.87ITGCGWATGLADD117 pKa = 3.61EE118 pKa = 4.96RR119 pKa = 11.84VVEE122 pKa = 4.35IAGCSWATGLADD134 pKa = 3.51EE135 pKa = 4.96RR136 pKa = 11.84VVEE139 pKa = 4.35IAGCSWAIEE148 pKa = 3.92LAAEE152 pKa = 4.14RR153 pKa = 11.84VEE155 pKa = 4.75EE156 pKa = 4.17IAGG159 pKa = 3.59

Molecular weight: 17.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A398CRA7|A0A398CRA7_9BACL Sugar ABC transporter permease OS=Cohnella faecalis OX=2315694 GN=D3H35_16720 PE=3 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.87PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.57KK14 pKa = 8.17VHH16 pKa = 5.5GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 10.35NGRR28 pKa = 11.84KK29 pKa = 8.13VLKK32 pKa = 10.34ARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.21GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5209

0

5209

1609417

17

3685

309.0

34.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.109 ± 0.042

0.753 ± 0.01

5.274 ± 0.026

6.593 ± 0.041

4.067 ± 0.027

7.77 ± 0.037

1.88 ± 0.017

6.098 ± 0.029

5.006 ± 0.031

9.963 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.022

3.603 ± 0.037

4.201 ± 0.026

3.359 ± 0.023

5.849 ± 0.051

6.813 ± 0.037

5.42 ± 0.05

7.156 ± 0.035

1.32 ± 0.015

3.278 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski