Glycine soja (Wild soybean)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; indigoferoid/millettioid clade;

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75060 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A445G958|A0A445G958_GLYSO 40S ribosomal protein S15-4 OS=Glycine soja OX=3848 GN=D0Y65_046405 PE=3 SV=1
MM1 pKa = 7.76ADD3 pKa = 2.79VTTYY7 pKa = 10.67LRR9 pKa = 11.84HH10 pKa = 6.46HH11 pKa = 7.57PDD13 pKa = 3.09SDD15 pKa = 4.17PNPNPNPNPNPNPDD29 pKa = 4.23DD30 pKa = 4.51DD31 pKa = 3.91QTLIPFPYY39 pKa = 9.11WDD41 pKa = 4.87LDD43 pKa = 3.55FDD45 pKa = 5.29FDD47 pKa = 5.46PEE49 pKa = 4.58FPSNTLSFTDD59 pKa = 4.82RR60 pKa = 11.84EE61 pKa = 4.22NQVNFIMDD69 pKa = 4.73LFHH72 pKa = 7.46QSVEE76 pKa = 4.16QSQLTDD82 pKa = 3.85PLSNDD87 pKa = 3.19AVFGAIDD94 pKa = 4.9GIDD97 pKa = 3.62LGFPAADD104 pKa = 3.82DD105 pKa = 4.09FFVGQRR111 pKa = 11.84FSVGSDD117 pKa = 3.01EE118 pKa = 5.08SHH120 pKa = 5.85THH122 pKa = 5.45PHH124 pKa = 5.6TLAANDD130 pKa = 3.95DD131 pKa = 4.18GVLGFCAHH139 pKa = 6.42SNEE142 pKa = 4.06NDD144 pKa = 3.61DD145 pKa = 3.72VASIPLCWDD154 pKa = 3.83ALQLEE159 pKa = 4.81EE160 pKa = 5.38NNNNNNTYY168 pKa = 11.1EE169 pKa = 4.07DD170 pKa = 4.43FEE172 pKa = 4.57WEE174 pKa = 4.05EE175 pKa = 4.15VMDD178 pKa = 4.11EE179 pKa = 4.17RR180 pKa = 11.84DD181 pKa = 4.3VISMLDD187 pKa = 3.48DD188 pKa = 3.67TVSVSLGIEE197 pKa = 4.2EE198 pKa = 4.11EE199 pKa = 4.5TEE201 pKa = 3.6AAAAEE206 pKa = 4.03EE207 pKa = 4.36DD208 pKa = 3.95AEE210 pKa = 4.65SEE212 pKa = 4.34VSILEE217 pKa = 4.08WQVLLNSTNLEE228 pKa = 4.29GPNSEE233 pKa = 4.82PYY235 pKa = 10.57FGDD238 pKa = 3.42SEE240 pKa = 5.02DD241 pKa = 3.78FVYY244 pKa = 9.68TAEE247 pKa = 4.07YY248 pKa = 11.07EE249 pKa = 4.32MMFGQFNDD257 pKa = 3.1NAFNGKK263 pKa = 8.54PPASASIVRR272 pKa = 11.84SLPSVVVTEE281 pKa = 4.61ADD283 pKa = 3.42VANDD287 pKa = 3.25NNVVVVCAVCKK298 pKa = 10.78DD299 pKa = 3.55EE300 pKa = 4.95FGVGEE305 pKa = 4.3GVKK308 pKa = 10.21VLPCSHH314 pKa = 7.35RR315 pKa = 11.84YY316 pKa = 9.2HH317 pKa = 7.02GEE319 pKa = 4.17CIVPWLGIRR328 pKa = 11.84NTCPVCRR335 pKa = 11.84YY336 pKa = 7.43EE337 pKa = 5.81FPTDD341 pKa = 3.36DD342 pKa = 3.85ADD344 pKa = 3.72YY345 pKa = 10.81EE346 pKa = 4.12RR347 pKa = 11.84RR348 pKa = 11.84KK349 pKa = 9.84AQRR352 pKa = 11.84SVMM355 pKa = 3.78

Molecular weight: 39.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A445GQT2|A0A445GQT2_GLYSO Glutaredoxin-C9 OS=Glycine soja OX=3848 GN=D0Y65_040273 PE=3 SV=1
MM1 pKa = 8.27ILMLGFGWIALRR13 pKa = 11.84GIIPPSLRR21 pKa = 11.84MISIHH26 pKa = 6.13RR27 pKa = 11.84RR28 pKa = 11.84LRR30 pKa = 11.84SSILPTLRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84FGSLKK45 pKa = 10.38FPAKK49 pKa = 9.66WNRR52 pKa = 11.84IWKK55 pKa = 9.29LNFWFGLGYY64 pKa = 10.47SRR66 pKa = 5.72

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55104

19956

75060

32485611

49

5288

432.8

48.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.523 ± 0.009

1.888 ± 0.004

5.281 ± 0.006

6.397 ± 0.009

4.229 ± 0.006

6.357 ± 0.009

2.543 ± 0.004

5.335 ± 0.007

6.169 ± 0.008

9.761 ± 0.011

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.004

4.708 ± 0.007

4.89 ± 0.008

3.794 ± 0.007

5.107 ± 0.006

9.032 ± 0.01

4.931 ± 0.006

6.556 ± 0.007

1.287 ± 0.003

2.808 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski