Spiroplasma sp. WSS

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; unclassified Spiroplasma

Average proteome isoelectric point is 7.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 648 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A533R6Y8|A0A533R6Y8_9MOLU Uncharacterized protein OS=Spiroplasma sp. WSS OX=2575700 GN=FCO83_01970 PE=4 SV=1
MM1 pKa = 7.45INKK4 pKa = 9.84KK5 pKa = 10.23IILSTCLGNIIGGSLFTLSDD25 pKa = 4.29LYY27 pKa = 10.77QKK29 pKa = 10.63RR30 pKa = 11.84EE31 pKa = 3.88QNILEE36 pKa = 3.97FDD38 pKa = 3.18ITSAITVLSGCFTGALFGFISGLFCCLNDD67 pKa = 3.64EE68 pKa = 4.81EE69 pKa = 6.11DD70 pKa = 3.47NGDD73 pKa = 3.3YY74 pKa = 11.11RR75 pKa = 11.84EE76 pKa = 4.21FFEE79 pKa = 4.52SQQIIDD85 pKa = 3.95NTHH88 pKa = 6.06SFQSSNEE95 pKa = 3.96FAPLLQEE102 pKa = 4.65YY103 pKa = 9.92ILEE106 pKa = 4.46INDD109 pKa = 3.32EE110 pKa = 4.05QFIPNNNADD119 pKa = 3.81LVFNPRR125 pKa = 11.84LVYY128 pKa = 10.67

Molecular weight: 14.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A533R896|A0A533R896_9MOLU 30S ribosomal protein S11 OS=Spiroplasma sp. WSS OX=2575700 GN=rpsK PE=3 SV=1
MM1 pKa = 7.57KK2 pKa = 8.61PTYY5 pKa = 9.87QPSKK9 pKa = 9.86RR10 pKa = 11.84KK11 pKa = 8.47HH12 pKa = 5.32QKK14 pKa = 6.68THH16 pKa = 5.1GFFARR21 pKa = 11.84MKK23 pKa = 9.52SATGINVIKK32 pKa = 10.5RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.11KK41 pKa = 9.68LTAA44 pKa = 4.27

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

648

0

648

192876

16

1536

297.6

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.993 ± 0.084

0.821 ± 0.024

4.796 ± 0.065

5.803 ± 0.101

4.952 ± 0.076

4.856 ± 0.09

1.604 ± 0.041

10.875 ± 0.104

9.427 ± 0.111

9.809 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.016 ± 0.035

8.347 ± 0.122

2.841 ± 0.049

3.796 ± 0.054

2.743 ± 0.059

6.15 ± 0.073

5.762 ± 0.072

5.443 ± 0.064

1.125 ± 0.04

3.842 ± 0.06
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski