Amino acid dipepetide frequency for Spiroplasma sp. WSS

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.355AlaAla: 3.355 ± 0.167
0.55AlaCys: 0.55 ± 0.06
2.105AlaAsp: 2.105 ± 0.116
2.074AlaGlu: 2.074 ± 0.129
2.297AlaPhe: 2.297 ± 0.14
3.049AlaGly: 3.049 ± 0.155
0.778AlaHis: 0.778 ± 0.063
5.75AlaIle: 5.75 ± 0.188
4.5AlaLys: 4.5 ± 0.178
5.273AlaLeu: 5.273 ± 0.174
1.047AlaMet: 1.047 ± 0.093
3.386AlaAsn: 3.386 ± 0.126
1.239AlaPro: 1.239 ± 0.082
1.804AlaGln: 1.804 ± 0.095
1.711AlaArg: 1.711 ± 0.093
2.717AlaSer: 2.717 ± 0.137
3.147AlaThr: 3.147 ± 0.15
2.971AlaVal: 2.971 ± 0.143
0.42AlaTrp: 0.42 ± 0.05
1.758AlaTyr: 1.758 ± 0.102
0.0AlaXaa: 0.0 ± 0.0
Cys
0.477CysAla: 0.477 ± 0.048
0.093CysCys: 0.093 ± 0.021
0.446CysAsp: 0.446 ± 0.049
0.513CysGlu: 0.513 ± 0.058
0.513CysPhe: 0.513 ± 0.06
0.513CysGly: 0.513 ± 0.051
0.187CysHis: 0.187 ± 0.029
0.674CysIle: 0.674 ± 0.059
0.684CysLys: 0.684 ± 0.054
0.887CysLeu: 0.887 ± 0.065
0.099CysMet: 0.099 ± 0.023
0.586CysAsn: 0.586 ± 0.053
0.233CysPro: 0.233 ± 0.033
0.436CysGln: 0.436 ± 0.047
0.161CysArg: 0.161 ± 0.029
0.544CysSer: 0.544 ± 0.053
0.358CysThr: 0.358 ± 0.045
0.394CysVal: 0.394 ± 0.041
0.109CysTrp: 0.109 ± 0.025
0.301CysTyr: 0.301 ± 0.038
0.0CysXaa: 0.0 ± 0.0
Asp
1.991AspAla: 1.991 ± 0.102
0.41AspCys: 0.41 ± 0.049
2.286AspAsp: 2.286 ± 0.124
3.261AspGlu: 3.261 ± 0.144
2.872AspPhe: 2.872 ± 0.119
2.302AspGly: 2.302 ± 0.121
0.674AspHis: 0.674 ± 0.06
5.045AspIle: 5.045 ± 0.148
4.158AspLys: 4.158 ± 0.181
5.47AspLeu: 5.47 ± 0.199
0.861AspMet: 0.861 ± 0.07
4.023AspAsn: 4.023 ± 0.164
1.358AspPro: 1.358 ± 0.09
1.457AspGln: 1.457 ± 0.089
1.011AspArg: 1.011 ± 0.079
2.675AspSer: 2.675 ± 0.13
2.369AspThr: 2.369 ± 0.119
2.655AspVal: 2.655 ± 0.128
0.638AspTrp: 0.638 ± 0.059
2.447AspTyr: 2.447 ± 0.117
0.0AspXaa: 0.0 ± 0.0
Glu
2.401GluAla: 2.401 ± 0.135
0.327GluCys: 0.327 ± 0.042
2.473GluAsp: 2.473 ± 0.137
4.075GluGlu: 4.075 ± 0.207
2.473GluPhe: 2.473 ± 0.113
2.089GluGly: 2.089 ± 0.129
0.933GluHis: 0.933 ± 0.067
6.942GluIle: 6.942 ± 0.194
6.429GluLys: 6.429 ± 0.222
5.537GluLeu: 5.537 ± 0.203
1.151GluMet: 1.151 ± 0.077
4.868GluAsn: 4.868 ± 0.168
1.192GluPro: 1.192 ± 0.086
3.225GluGln: 3.225 ± 0.145
1.846GluArg: 1.846 ± 0.118
2.598GluSer: 2.598 ± 0.141
3.1GluThr: 3.1 ± 0.131
3.66GluVal: 3.66 ± 0.15
0.524GluTrp: 0.524 ± 0.055
2.178GluTyr: 2.178 ± 0.104
0.0GluXaa: 0.0 ± 0.0
Phe
2.649PheAla: 2.649 ± 0.13
0.482PheCys: 0.482 ± 0.053
2.722PheAsp: 2.722 ± 0.105
2.395PheGlu: 2.395 ± 0.119
2.566PhePhe: 2.566 ± 0.169
2.592PheGly: 2.592 ± 0.126
0.674PheHis: 0.674 ± 0.061
5.2PheIle: 5.2 ± 0.186
4.36PheLys: 4.36 ± 0.146
5.242PheLeu: 5.242 ± 0.187
1.021PheMet: 1.021 ± 0.074
4.283PheAsn: 4.283 ± 0.165
1.172PhePro: 1.172 ± 0.087
1.695PheGln: 1.695 ± 0.082
1.135PheArg: 1.135 ± 0.074
3.671PheSer: 3.671 ± 0.146
2.841PheThr: 2.841 ± 0.142
2.359PheVal: 2.359 ± 0.111
0.586PheTrp: 0.586 ± 0.063
1.877PheTyr: 1.877 ± 0.094
0.0PheXaa: 0.0 ± 0.0
Gly
2.732GlyAla: 2.732 ± 0.15
0.342GlyCys: 0.342 ± 0.04
2.141GlyAsp: 2.141 ± 0.117
2.354GlyGlu: 2.354 ± 0.118
2.66GlyPhe: 2.66 ± 0.108
2.919GlyGly: 2.919 ± 0.187
1.021GlyHis: 1.021 ± 0.074
5.402GlyIle: 5.402 ± 0.185
4.454GlyLys: 4.454 ± 0.184
4.5GlyLeu: 4.5 ± 0.156
1.192GlyMet: 1.192 ± 0.086
2.831GlyAsn: 2.831 ± 0.123
1.11GlyPro: 1.11 ± 0.088
1.638GlyGln: 1.638 ± 0.091
1.555GlyArg: 1.555 ± 0.099
2.883GlySer: 2.883 ± 0.15
3.106GlyThr: 3.106 ± 0.178
3.033GlyVal: 3.033 ± 0.15
0.643GlyTrp: 0.643 ± 0.067
2.048GlyTyr: 2.048 ± 0.105
0.0GlyXaa: 0.0 ± 0.0
His
0.866HisAla: 0.866 ± 0.064
0.171HisCys: 0.171 ± 0.03
0.835HisAsp: 0.835 ± 0.068
0.835HisGlu: 0.835 ± 0.065
0.747HisPhe: 0.747 ± 0.067
0.933HisGly: 0.933 ± 0.082
0.482HisHis: 0.482 ± 0.053
1.415HisIle: 1.415 ± 0.083
1.161HisLys: 1.161 ± 0.071
1.69HisLeu: 1.69 ± 0.101
0.249HisMet: 0.249 ± 0.038
1.135HisAsn: 1.135 ± 0.089
0.71HisPro: 0.71 ± 0.061
0.918HisGln: 0.918 ± 0.064
0.534HisArg: 0.534 ± 0.049
0.944HisSer: 0.944 ± 0.077
0.674HisThr: 0.674 ± 0.062
0.83HisVal: 0.83 ± 0.067
0.161HisTrp: 0.161 ± 0.03
0.752HisTyr: 0.752 ± 0.067
0.0HisXaa: 0.0 ± 0.0
Ile
5.973IleAla: 5.973 ± 0.182
1.094IleCys: 1.094 ± 0.084
5.677IleAsp: 5.677 ± 0.169
5.797IleGlu: 5.797 ± 0.204
5.071IlePhe: 5.071 ± 0.205
5.195IleGly: 5.195 ± 0.205
1.472IleHis: 1.472 ± 0.093
12.35IleIle: 12.35 ± 0.337
10.769IleLys: 10.769 ± 0.258
9.908IleLeu: 9.908 ± 0.274
2.146IleMet: 2.146 ± 0.104
10.94IleAsn: 10.94 ± 0.336
3.225IlePro: 3.225 ± 0.141
2.39IleGln: 2.39 ± 0.117
2.831IleArg: 2.831 ± 0.126
7.342IleSer: 7.342 ± 0.238
6.693IleThr: 6.693 ± 0.21
5.631IleVal: 5.631 ± 0.172
0.928IleTrp: 0.928 ± 0.076
3.318IleTyr: 3.318 ± 0.134
0.0IleXaa: 0.0 ± 0.0
Lys
3.889LysAla: 3.889 ± 0.183
0.56LysCys: 0.56 ± 0.056
4.651LysAsp: 4.651 ± 0.175
6.719LysGlu: 6.719 ± 0.207
3.655LysPhe: 3.655 ± 0.166
3.479LysGly: 3.479 ± 0.165
1.426LysHis: 1.426 ± 0.084
10.841LysIle: 10.841 ± 0.298
9.623LysLys: 9.623 ± 0.277
8.057LysLeu: 8.057 ± 0.212
2.338LysMet: 2.338 ± 0.124
8.576LysAsn: 8.576 ± 0.245
2.665LysPro: 2.665 ± 0.117
4.106LysGln: 4.106 ± 0.155
2.421LysArg: 2.421 ± 0.117
5.003LysSer: 5.003 ± 0.185
5.911LysThr: 5.911 ± 0.208
5.34LysVal: 5.34 ± 0.171
1.12LysTrp: 1.12 ± 0.081
3.889LysTyr: 3.889 ± 0.14
0.0LysXaa: 0.0 ± 0.0
Leu
5.408LeuAla: 5.408 ± 0.186
0.757LeuCys: 0.757 ± 0.069
4.226LeuAsp: 4.226 ± 0.147
5.734LeuGlu: 5.734 ± 0.216
4.677LeuPhe: 4.677 ± 0.19
4.879LeuGly: 4.879 ± 0.217
1.343LeuHis: 1.343 ± 0.084
10.333LeuIle: 10.333 ± 0.268
9.239LeuLys: 9.239 ± 0.228
9.581LeuLeu: 9.581 ± 0.282
2.105LeuMet: 2.105 ± 0.11
7.259LeuAsn: 7.259 ± 0.225
3.329LeuPro: 3.329 ± 0.135
3.961LeuGln: 3.961 ± 0.182
2.649LeuArg: 2.649 ± 0.13
6.683LeuSer: 6.683 ± 0.188
6.331LeuThr: 6.331 ± 0.175
5.812LeuVal: 5.812 ± 0.186
0.938LeuTrp: 0.938 ± 0.075
2.852LeuTyr: 2.852 ± 0.126
0.0LeuXaa: 0.0 ± 0.0
Met
1.172MetAla: 1.172 ± 0.066
0.156MetCys: 0.156 ± 0.029
0.674MetAsp: 0.674 ± 0.053
1.135MetGlu: 1.135 ± 0.08
1.001MetPhe: 1.001 ± 0.085
0.985MetGly: 0.985 ± 0.069
0.296MetHis: 0.296 ± 0.043
2.224MetIle: 2.224 ± 0.126
1.908MetLys: 1.908 ± 0.113
2.126MetLeu: 2.126 ± 0.106
0.456MetMet: 0.456 ± 0.054
1.436MetAsn: 1.436 ± 0.088
0.814MetPro: 0.814 ± 0.058
0.83MetGln: 0.83 ± 0.067
0.591MetArg: 0.591 ± 0.054
1.353MetSer: 1.353 ± 0.083
1.094MetThr: 1.094 ± 0.066
1.027MetVal: 1.027 ± 0.077
0.223MetTrp: 0.223 ± 0.038
0.664MetTyr: 0.664 ± 0.057
0.0MetXaa: 0.0 ± 0.0
Asn
3.126AsnAla: 3.126 ± 0.159
0.658AsnCys: 0.658 ± 0.059
4.635AsnAsp: 4.635 ± 0.186
4.894AsnGlu: 4.894 ± 0.16
4.376AsnPhe: 4.376 ± 0.164
3.37AsnGly: 3.37 ± 0.176
1.483AsnHis: 1.483 ± 0.098
9.4AsnIle: 9.4 ± 0.24
8.451AsnLys: 8.451 ± 0.243
7.642AsnLeu: 7.642 ± 0.237
1.224AsnMet: 1.224 ± 0.091
8.985AsnAsn: 8.985 ± 0.316
2.592AsnPro: 2.592 ± 0.126
3.334AsnGln: 3.334 ± 0.144
2.048AsnArg: 2.048 ± 0.116
5.076AsnSer: 5.076 ± 0.201
3.52AsnThr: 3.52 ± 0.177
3.894AsnVal: 3.894 ± 0.151
1.11AsnTrp: 1.11 ± 0.088
3.65AsnTyr: 3.65 ± 0.172
0.0AsnXaa: 0.0 ± 0.0
Pro
1.156ProAla: 1.156 ± 0.083
0.181ProCys: 0.181 ± 0.035
1.307ProAsp: 1.307 ± 0.092
1.721ProGlu: 1.721 ± 0.101
1.592ProPhe: 1.592 ± 0.109
1.296ProGly: 1.296 ± 0.088
0.487ProHis: 0.487 ± 0.053
3.043ProIle: 3.043 ± 0.126
2.375ProLys: 2.375 ± 0.113
2.919ProLeu: 2.919 ± 0.123
0.544ProMet: 0.544 ± 0.056
2.38ProAsn: 2.38 ± 0.116
0.705ProPro: 0.705 ± 0.057
1.135ProGln: 1.135 ± 0.072
0.653ProArg: 0.653 ± 0.062
1.825ProSer: 1.825 ± 0.095
1.882ProThr: 1.882 ± 0.109
1.644ProVal: 1.644 ± 0.09
0.394ProTrp: 0.394 ± 0.051
1.167ProTyr: 1.167 ± 0.078
0.0ProXaa: 0.0 ± 0.0
Gln
1.763GlnAla: 1.763 ± 0.104
0.192GlnCys: 0.192 ± 0.031
1.716GlnAsp: 1.716 ± 0.093
2.758GlnGlu: 2.758 ± 0.115
1.908GlnPhe: 1.908 ± 0.097
1.457GlnGly: 1.457 ± 0.1
0.627GlnHis: 0.627 ± 0.063
3.707GlnIle: 3.707 ± 0.163
3.634GlnLys: 3.634 ± 0.151
4.48GlnLeu: 4.48 ± 0.168
0.83GlnMet: 0.83 ± 0.072
2.686GlnAsn: 2.686 ± 0.123
0.949GlnPro: 0.949 ± 0.065
1.965GlnGln: 1.965 ± 0.1
1.146GlnArg: 1.146 ± 0.079
1.991GlnSer: 1.991 ± 0.109
1.949GlnThr: 1.949 ± 0.094
2.048GlnVal: 2.048 ± 0.098
0.529GlnTrp: 0.529 ± 0.059
1.623GlnTyr: 1.623 ± 0.093
0.0GlnXaa: 0.0 ± 0.0
Arg
1.307ArgAla: 1.307 ± 0.094
0.207ArgCys: 0.207 ± 0.034
1.384ArgAsp: 1.384 ± 0.091
1.664ArgGlu: 1.664 ± 0.116
1.332ArgPhe: 1.332 ± 0.079
1.436ArgGly: 1.436 ± 0.096
0.451ArgHis: 0.451 ± 0.051
2.82ArgIle: 2.82 ± 0.123
2.67ArgLys: 2.67 ± 0.132
2.406ArgLeu: 2.406 ± 0.116
0.804ArgMet: 0.804 ± 0.069
2.157ArgAsn: 2.157 ± 0.108
0.788ArgPro: 0.788 ± 0.063
1.099ArgGln: 1.099 ± 0.082
0.954ArgArg: 0.954 ± 0.093
1.488ArgSer: 1.488 ± 0.089
1.529ArgThr: 1.529 ± 0.094
1.514ArgVal: 1.514 ± 0.084
0.311ArgTrp: 0.311 ± 0.036
1.11ArgTyr: 1.11 ± 0.079
0.0ArgXaa: 0.0 ± 0.0
Ser
3.075SerAla: 3.075 ± 0.144
0.596SerCys: 0.596 ± 0.057
3.152SerAsp: 3.152 ± 0.151
3.764SerGlu: 3.764 ± 0.136
3.054SerPhe: 3.054 ± 0.129
3.494SerGly: 3.494 ± 0.153
1.027SerHis: 1.027 ± 0.077
5.76SerIle: 5.76 ± 0.167
5.558SerLys: 5.558 ± 0.179
6.73SerLeu: 6.73 ± 0.208
0.985SerMet: 0.985 ± 0.078
4.744SerAsn: 4.744 ± 0.185
1.472SerPro: 1.472 ± 0.082
2.214SerGln: 2.214 ± 0.109
1.737SerArg: 1.737 ± 0.106
4.469SerSer: 4.469 ± 0.182
3.448SerThr: 3.448 ± 0.136
2.96SerVal: 2.96 ± 0.14
0.84SerTrp: 0.84 ± 0.07
2.416SerTyr: 2.416 ± 0.124
0.0SerXaa: 0.0 ± 0.0
Thr
2.94ThrAla: 2.94 ± 0.131
0.43ThrCys: 0.43 ± 0.048
2.473ThrAsp: 2.473 ± 0.122
2.338ThrGlu: 2.338 ± 0.129
3.049ThrPhe: 3.049 ± 0.145
2.986ThrGly: 2.986 ± 0.127
0.845ThrHis: 0.845 ± 0.074
6.916ThrIle: 6.916 ± 0.203
5.361ThrLys: 5.361 ± 0.19
5.568ThrLeu: 5.568 ± 0.168
1.032ThrMet: 1.032 ± 0.077
5.133ThrAsn: 5.133 ± 0.205
1.815ThrPro: 1.815 ± 0.116
1.535ThrGln: 1.535 ± 0.1
1.509ThrArg: 1.509 ± 0.103
3.852ThrSer: 3.852 ± 0.145
4.039ThrThr: 4.039 ± 0.17
3.012ThrVal: 3.012 ± 0.133
0.539ThrTrp: 0.539 ± 0.054
2.245ThrTyr: 2.245 ± 0.125
0.0ThrXaa: 0.0 ± 0.0
Val
3.36ValAla: 3.36 ± 0.147
0.472ValCys: 0.472 ± 0.05
2.437ValAsp: 2.437 ± 0.123
3.059ValGlu: 3.059 ± 0.137
2.525ValPhe: 2.525 ± 0.109
3.075ValGly: 3.075 ± 0.158
0.773ValHis: 0.773 ± 0.071
6.476ValIle: 6.476 ± 0.198
4.635ValLys: 4.635 ± 0.17
5.107ValLeu: 5.107 ± 0.161
1.135ValMet: 1.135 ± 0.088
4.054ValAsn: 4.054 ± 0.178
1.669ValPro: 1.669 ± 0.091
1.509ValGln: 1.509 ± 0.092
1.462ValArg: 1.462 ± 0.093
3.489ValSer: 3.489 ± 0.141
3.443ValThr: 3.443 ± 0.133
3.365ValVal: 3.365 ± 0.163
0.518ValTrp: 0.518 ± 0.048
1.866ValTyr: 1.866 ± 0.09
0.0ValXaa: 0.0 ± 0.0
Trp
0.42TrpAla: 0.42 ± 0.046
0.093TrpCys: 0.093 ± 0.023
0.601TrpAsp: 0.601 ± 0.059
0.591TrpGlu: 0.591 ± 0.052
0.607TrpPhe: 0.607 ± 0.062
0.508TrpGly: 0.508 ± 0.05
0.176TrpHis: 0.176 ± 0.033
1.182TrpIle: 1.182 ± 0.091
1.244TrpLys: 1.244 ± 0.084
0.97TrpLeu: 0.97 ± 0.079
0.259TrpMet: 0.259 ± 0.039
1.13TrpAsn: 1.13 ± 0.094
0.285TrpPro: 0.285 ± 0.042
0.43TrpGln: 0.43 ± 0.046
0.238TrpArg: 0.238 ± 0.035
0.627TrpSer: 0.627 ± 0.053
0.69TrpThr: 0.69 ± 0.073
0.555TrpVal: 0.555 ± 0.058
0.259TrpTrp: 0.259 ± 0.037
0.378TrpTyr: 0.378 ± 0.046
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.872TyrAla: 1.872 ± 0.112
0.436TyrCys: 0.436 ± 0.05
2.022TyrAsp: 2.022 ± 0.109
2.343TyrGlu: 2.343 ± 0.131
2.551TyrPhe: 2.551 ± 0.106
2.006TyrGly: 2.006 ± 0.114
0.887TyrHis: 0.887 ± 0.072
3.266TyrIle: 3.266 ± 0.141
3.054TyrLys: 3.054 ± 0.133
4.003TyrLeu: 4.003 ± 0.148
0.679TyrMet: 0.679 ± 0.053
2.878TyrAsn: 2.878 ± 0.149
1.084TyrPro: 1.084 ± 0.086
2.276TyrGln: 2.276 ± 0.132
1.239TyrArg: 1.239 ± 0.081
2.266TyrSer: 2.266 ± 0.115
1.493TyrThr: 1.493 ± 0.101
1.727TyrVal: 1.727 ± 0.098
0.456TyrTrp: 0.456 ± 0.052
1.882TyrTyr: 1.882 ± 0.115
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 648 proteins (192876 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski