Silicibacter phage DSS3phi2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Rhodovirinae; Aorunvirus; unclassified Aorunvirus

Average proteome isoelectric point is 5.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4NT47|C4NT47_9CAUD N4 gp14-like protein OS=Silicibacter phage DSS3phi2 OX=490912 PE=4 SV=1
MM1 pKa = 7.71APMGLLLAACGSPPEE16 pKa = 4.09ICDD19 pKa = 4.94RR20 pKa = 11.84EE21 pKa = 4.25LQTWDD26 pKa = 3.19KK27 pKa = 10.68FQTEE31 pKa = 4.37EE32 pKa = 4.3DD33 pKa = 3.83QCDD36 pKa = 3.65GTSNHH41 pKa = 5.93VYY43 pKa = 10.59SSGPDD48 pKa = 2.87WTYY51 pKa = 11.07TGGASDD57 pKa = 5.29SDD59 pKa = 4.28TEE61 pKa = 4.37SDD63 pKa = 5.75SSDD66 pKa = 3.42EE67 pKa = 4.93DD68 pKa = 3.65NGSDD72 pKa = 4.41SDD74 pKa = 4.03NGGSNSGPPSDD85 pKa = 5.08GGSDD89 pKa = 4.85DD90 pKa = 5.36SGDD93 pKa = 4.01DD94 pKa = 3.42NGGDD98 pKa = 3.76SDD100 pKa = 4.93HH101 pKa = 7.71DD102 pKa = 4.44GGSDD106 pKa = 3.57SPGSGGTDD114 pKa = 3.01DD115 pKa = 4.52GGKK118 pKa = 8.68NHH120 pKa = 6.8GNNGHH125 pKa = 6.52GNDD128 pKa = 4.78DD129 pKa = 4.66DD130 pKa = 4.81GHH132 pKa = 6.76DD133 pKa = 3.88SSNPGKK139 pKa = 10.77GHH141 pKa = 7.08GGPNGNPDD149 pKa = 3.25GTDD152 pKa = 3.11QDD154 pKa = 3.84GSPGKK159 pKa = 10.83GKK161 pKa = 9.97GHH163 pKa = 7.0

Molecular weight: 16.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4NT08|C4NT08_9CAUD N4 RNAP1-like protein OS=Silicibacter phage DSS3phi2 OX=490912 PE=4 SV=1
MM1 pKa = 8.08PDD3 pKa = 3.53GLSKK7 pKa = 11.24ANARR11 pKa = 11.84QSSKK15 pKa = 6.98WQRR18 pKa = 11.84SSKK21 pKa = 9.69SRR23 pKa = 11.84RR24 pKa = 11.84AANLPAKK31 pKa = 10.05AGRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.79PPAAKK41 pKa = 9.67KK42 pKa = 9.46GKK44 pKa = 9.76HH45 pKa = 4.48ITTHH49 pKa = 5.72EE50 pKa = 3.86EE51 pKa = 3.62RR52 pKa = 11.84QRR54 pKa = 11.84GIFLTQFGLKK64 pKa = 10.08PFVNPWINRR73 pKa = 11.84KK74 pKa = 8.71GPKK77 pKa = 9.57EE78 pKa = 3.97SVGEE82 pKa = 4.12DD83 pKa = 3.14EE84 pKa = 5.13FAA86 pKa = 5.27

Molecular weight: 9.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

23038

35

3632

284.4

31.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.978 ± 0.315

0.833 ± 0.164

6.507 ± 0.184

6.819 ± 0.245

3.967 ± 0.139

7.279 ± 0.25

1.927 ± 0.218

5.669 ± 0.184

5.434 ± 0.309

7.891 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.29 ± 0.171

5.252 ± 0.176

4.349 ± 0.201

4.358 ± 0.197

5.026 ± 0.19

6.068 ± 0.173

6.181 ± 0.197

6.611 ± 0.261

1.402 ± 0.158

3.156 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski