Pseudoalteromonas piratica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3997 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7EB23|A0A0A7EB23_9GAMM Iron-sulfur cluster assembly scaffold protein IscU OS=Pseudoalteromonas piratica OX=1348114 GN=OM33_00190 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 10.37KK3 pKa = 10.36IMFGAALIAATLAPQAKK20 pKa = 10.05ADD22 pKa = 4.1TLLGLYY28 pKa = 10.22VGGDD32 pKa = 3.22YY33 pKa = 9.91WAAQSSGGLGTSSDD47 pKa = 3.41LQNFNFEE54 pKa = 4.1DD55 pKa = 3.68QEE57 pKa = 4.36YY58 pKa = 9.57GSYY61 pKa = 10.22YY62 pKa = 10.66AALEE66 pKa = 4.13HH67 pKa = 6.98PIPLVPNIKK76 pKa = 10.21LKK78 pKa = 10.94YY79 pKa = 9.38NQLEE83 pKa = 4.32LNADD87 pKa = 3.4ATLNTSFGFGDD98 pKa = 3.65KK99 pKa = 10.48VFKK102 pKa = 10.92VNSIVTGDD110 pKa = 3.5SDD112 pKa = 4.43LSHH115 pKa = 7.39VDD117 pKa = 3.09YY118 pKa = 11.27VLYY121 pKa = 11.13YY122 pKa = 10.38EE123 pKa = 5.58IFDD126 pKa = 4.13NDD128 pKa = 4.15LVSIDD133 pKa = 4.69LGLSAKK139 pKa = 10.33QFDD142 pKa = 4.51GYY144 pKa = 10.63IAVKK148 pKa = 10.66GDD150 pKa = 3.79DD151 pKa = 3.4QSMGMSEE158 pKa = 4.26EE159 pKa = 4.29SVDD162 pKa = 4.17FNGIIPMAYY171 pKa = 9.61GAVQFGLPFTGLSAFAEE188 pKa = 4.67GQLLAIGDD196 pKa = 4.35SKK198 pKa = 10.87IQDD201 pKa = 3.84YY202 pKa = 10.49QVGVAWEE209 pKa = 5.09FIDD212 pKa = 4.96NMAVDD217 pKa = 4.23VAVKK221 pKa = 10.21AGYY224 pKa = 9.83RR225 pKa = 11.84AMLLEE230 pKa = 5.33LDD232 pKa = 4.74DD233 pKa = 4.92VDD235 pKa = 6.39DD236 pKa = 4.8IYY238 pKa = 11.52TDD240 pKa = 3.67VEE242 pKa = 4.01VDD244 pKa = 3.53GFFAGLQVHH253 pKa = 6.54FF254 pKa = 4.92

Molecular weight: 27.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7EC85|A0A0A7EC85_9GAMM Uncharacterized protein OS=Pseudoalteromonas piratica OX=1348114 GN=OM33_03250 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3997

0

3997

1331845

44

2642

333.2

37.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.539 ± 0.038

0.975 ± 0.014

5.526 ± 0.033

6.306 ± 0.042

4.483 ± 0.026

6.267 ± 0.04

2.189 ± 0.02

6.561 ± 0.03

5.959 ± 0.036

10.456 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.02

5.082 ± 0.028

3.535 ± 0.021

4.622 ± 0.035

3.935 ± 0.025

6.852 ± 0.039

5.404 ± 0.029

6.674 ± 0.033

1.111 ± 0.013

3.252 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski