Tuhoko virus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Rubulavirinae; Pararubulavirus; Tuhoko pararubulavirus 3

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8WJ37|D8WJ37_9MONO Phosphoprotein OS=Tuhoko virus 3 OX=798074 GN=V/P PE=3 SV=1
MM1 pKa = 7.63ASLFRR6 pKa = 11.84ALEE9 pKa = 4.01QFTLEE14 pKa = 3.83QDD16 pKa = 2.79QGGRR20 pKa = 11.84GNVGDD25 pKa = 4.39QPPEE29 pKa = 3.9TLQATIKK36 pKa = 10.49VFVINTPDD44 pKa = 2.96PRR46 pKa = 11.84LRR48 pKa = 11.84YY49 pKa = 10.36RR50 pKa = 11.84MMCFCLRR57 pKa = 11.84LIVSNSARR65 pKa = 11.84TGQRR69 pKa = 11.84HH70 pKa = 5.2GALMTLLSLPTAVMQNHH87 pKa = 5.7FRR89 pKa = 11.84TAEE92 pKa = 3.95RR93 pKa = 11.84SPDD96 pKa = 3.6CQIEE100 pKa = 4.28RR101 pKa = 11.84IEE103 pKa = 4.03VDD105 pKa = 3.04GFEE108 pKa = 4.54PGTYY112 pKa = 9.68RR113 pKa = 11.84IRR115 pKa = 11.84TNARR119 pKa = 11.84TPLTHH124 pKa = 6.63GEE126 pKa = 4.27VVALEE131 pKa = 4.17EE132 pKa = 4.17MADD135 pKa = 4.56DD136 pKa = 4.66IPEE139 pKa = 4.17ALANHH144 pKa = 6.27TPFVDD149 pKa = 3.4AQTEE153 pKa = 4.25LEE155 pKa = 4.45EE156 pKa = 5.24CDD158 pKa = 3.62EE159 pKa = 4.19MEE161 pKa = 4.74KK162 pKa = 10.56FLEE165 pKa = 5.01AIYY168 pKa = 9.24STLIQVWIMVTKK180 pKa = 10.91SMTAFDD186 pKa = 4.14QPTGSDD192 pKa = 3.06EE193 pKa = 4.23RR194 pKa = 11.84RR195 pKa = 11.84VAKK198 pKa = 9.97YY199 pKa = 8.5QQQGRR204 pKa = 11.84LNPHH208 pKa = 6.3YY209 pKa = 10.19LLQGEE214 pKa = 4.44VRR216 pKa = 11.84RR217 pKa = 11.84RR218 pKa = 11.84IQLCIRR224 pKa = 11.84QSLPIRR230 pKa = 11.84QFLVNEE236 pKa = 4.35LQTASNQGPITGKK249 pKa = 10.1YY250 pKa = 7.77YY251 pKa = 11.55AMVADD256 pKa = 3.46IGKK259 pKa = 10.03YY260 pKa = 9.88IMNAGMGGFFMTIRR274 pKa = 11.84FALGQKK280 pKa = 8.73WPPLALAAFSGEE292 pKa = 3.91IVKK295 pKa = 9.67MKK297 pKa = 10.77SLMLQYY303 pKa = 10.52RR304 pKa = 11.84RR305 pKa = 11.84LGDD308 pKa = 3.3RR309 pKa = 11.84AKK311 pKa = 10.92FMALLEE317 pKa = 4.23MSEE320 pKa = 4.33MMDD323 pKa = 3.59FAPSNFPLLYY333 pKa = 9.8SYY335 pKa = 11.8AMGIGSVQDD344 pKa = 3.55PQMRR348 pKa = 11.84GYY350 pKa = 9.17TFGRR354 pKa = 11.84PYY356 pKa = 10.92LNAAFYY362 pKa = 10.48QLGVEE367 pKa = 4.61TANQQQGSVDD377 pKa = 3.17KK378 pKa = 11.53DD379 pKa = 3.21MAAEE383 pKa = 4.42LGLTEE388 pKa = 4.17ADD390 pKa = 3.59KK391 pKa = 11.18RR392 pKa = 11.84AMAATVTRR400 pKa = 11.84LTTGRR405 pKa = 11.84GGLQGNMGINAMARR419 pKa = 11.84RR420 pKa = 11.84GQQLPQPQDD429 pKa = 3.21NVVEE433 pKa = 4.09EE434 pKa = 4.65DD435 pKa = 3.58EE436 pKa = 5.03EE437 pKa = 4.3EE438 pKa = 4.54DD439 pKa = 3.84EE440 pKa = 5.51EE441 pKa = 5.06EE442 pKa = 4.91EE443 pKa = 4.28EE444 pKa = 4.24PQNQEE449 pKa = 4.0RR450 pKa = 11.84EE451 pKa = 4.03VDD453 pKa = 3.49PEE455 pKa = 4.05LEE457 pKa = 3.83RR458 pKa = 11.84RR459 pKa = 11.84VALRR463 pKa = 11.84IAQDD467 pKa = 3.2QEE469 pKa = 3.29RR470 pKa = 11.84WARR473 pKa = 11.84RR474 pKa = 11.84LAEE477 pKa = 3.99IEE479 pKa = 3.95AEE481 pKa = 4.16RR482 pKa = 11.84VARR485 pKa = 11.84QAASHH490 pKa = 7.01PPQHH494 pKa = 7.12DD495 pKa = 3.44ATRR498 pKa = 11.84DD499 pKa = 3.62QIPDD503 pKa = 3.34QQEE506 pKa = 3.79YY507 pKa = 10.96DD508 pKa = 3.74SNLIEE513 pKa = 4.2

Molecular weight: 58.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8WJ40|D8WJ40_9MONO Fusion glycoprotein F0 OS=Tuhoko virus 3 OX=798074 GN=F PE=3 SV=1
MM1 pKa = 8.02AGRR4 pKa = 11.84QATIPVPINFEE15 pKa = 4.1SPKK18 pKa = 10.51NYY20 pKa = 10.39LNAFPIVQADD30 pKa = 3.58PSVSGEE36 pKa = 3.79AGKK39 pKa = 10.23LLKK42 pKa = 10.27QIRR45 pKa = 11.84FKK47 pKa = 11.3DD48 pKa = 3.34LTLRR52 pKa = 11.84GSTEE56 pKa = 3.84APISFVNSYY65 pKa = 11.02GFIKK69 pKa = 10.27PLRR72 pKa = 11.84TRR74 pKa = 11.84EE75 pKa = 3.92EE76 pKa = 4.52FFSEE80 pKa = 3.8MHH82 pKa = 6.62KK83 pKa = 10.54PSQAPCLTACCLPFGAGPAIEE104 pKa = 5.06HH105 pKa = 7.27PDD107 pKa = 4.28KK108 pKa = 11.44IMDD111 pKa = 4.58DD112 pKa = 3.59LDD114 pKa = 3.5KK115 pKa = 11.59VFIVVRR121 pKa = 11.84KK122 pKa = 8.48SASTIEE128 pKa = 3.99EE129 pKa = 4.4CVFDD133 pKa = 3.97IRR135 pKa = 11.84KK136 pKa = 9.34LPSSLSRR143 pKa = 11.84HH144 pKa = 4.48QLAGNRR150 pKa = 11.84VLCVASDD157 pKa = 3.84KK158 pKa = 11.02YY159 pKa = 11.05IKK161 pKa = 10.46APCKK165 pKa = 9.03LTSGMDD171 pKa = 3.18YY172 pKa = 10.04TYY174 pKa = 11.03NIVFLSITHH183 pKa = 6.78CPPSQKK189 pKa = 9.82FRR191 pKa = 11.84VPMPIQSLRR200 pKa = 11.84AKK202 pKa = 10.31VMRR205 pKa = 11.84SVHH208 pKa = 6.84LEE210 pKa = 3.32IMIKK214 pKa = 9.99VDD216 pKa = 3.63CDD218 pKa = 3.53KK219 pKa = 11.31NSPITKK225 pKa = 9.97NLIYY229 pKa = 10.67DD230 pKa = 4.19PSNDD234 pKa = 2.29IWMASIWFHH243 pKa = 6.49LCNFYY248 pKa = 10.75KK249 pKa = 10.53GSKK252 pKa = 8.91PFKK255 pKa = 10.3EE256 pKa = 4.09YY257 pKa = 10.65DD258 pKa = 3.59DD259 pKa = 3.38QHH261 pKa = 6.17FASKK265 pKa = 9.8CRR267 pKa = 11.84AMGLEE272 pKa = 4.02VGLVDD277 pKa = 3.92FWGPTFLVKK286 pKa = 10.56AHH288 pKa = 6.08GKK290 pKa = 9.03IPHH293 pKa = 6.69AARR296 pKa = 11.84PFFGKK301 pKa = 9.88HH302 pKa = 4.5GWVCHH307 pKa = 5.64PMMDD311 pKa = 4.2CAPAISKK318 pKa = 9.21SLWALSISILQVNAVLQASDD338 pKa = 4.18LNQMIRR344 pKa = 11.84MTDD347 pKa = 3.23VVFPKK352 pKa = 10.83VKK354 pKa = 10.17INPDD358 pKa = 2.73IAGVQKK364 pKa = 9.66TRR366 pKa = 11.84WNPVKK371 pKa = 10.67KK372 pKa = 10.35LVTIDD377 pKa = 3.08

Molecular weight: 42.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

1

7

4925

244

2271

703.6

78.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.558 ± 0.89

2.091 ± 0.359

4.731 ± 0.523

5.401 ± 0.588

3.492 ± 0.283

5.036 ± 0.366

2.213 ± 0.362

7.777 ± 0.638

4.832 ± 0.725

10.457 ± 1.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.944 ± 0.336

5.137 ± 0.472

5.442 ± 0.78

4.65 ± 0.849

4.995 ± 0.456

8.751 ± 0.694

6.112 ± 0.464

5.442 ± 0.448

1.015 ± 0.161

2.924 ± 0.406
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski