Actinidia virus 1
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A223A477|A0A223A477_9CLOS Leader protease OS=Actinidia virus 1 OX=2024724 PE=4 SV=1
MM1 pKa = 8.4 DD2 pKa = 5.15 EE3 pKa = 4.33 LLQVAFSMLFLLGFCLLLCTCLVGSFSVYY32 pKa = 10.67 KK33 pKa = 10.51 KK34 pKa = 11.0 VSDD37 pKa = 3.95 PDD39 pKa = 3.49 AATEE43 pKa = 4.22 NISRR47 pKa = 11.84 VGRR50 pKa = 11.84 LL51 pKa = 3.07
Molecular weight: 5.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.855
IPC2_protein 5.27
IPC_protein 4.749
Toseland 4.571
ProMoST 4.876
Dawson 4.711
Bjellqvist 4.863
Wikipedia 4.622
Rodwell 4.584
Grimsley 4.495
Solomon 4.685
Lehninger 4.647
Nozaki 4.838
DTASelect 5.003
Thurlkill 4.622
EMBOSS 4.635
Sillero 4.851
Patrickios 3.503
IPC_peptide 4.698
IPC2_peptide 4.838
IPC2.peptide.svr19 4.949
Protein with the highest isoelectric point:
>tr|A0A223A3K8|A0A223A3K8_9CLOS Uncharacterized protein OS=Actinidia virus 1 OX=2024724 PE=4 SV=1
MM1 pKa = 7.35 GALFTVYY8 pKa = 10.0 EE9 pKa = 4.38 SEE11 pKa = 4.23 RR12 pKa = 11.84 HH13 pKa = 3.91 VAYY16 pKa = 10.42 DD17 pKa = 3.04 NRR19 pKa = 11.84 DD20 pKa = 3.31 RR21 pKa = 11.84 HH22 pKa = 5.9 PSWGSSCHH30 pKa = 6.36 YY31 pKa = 8.87 DD32 pKa = 2.99 TYY34 pKa = 11.73 YY35 pKa = 11.26 NNCGSGGPYY44 pKa = 10.12 GRR46 pKa = 11.84 STDD49 pKa = 3.88 SLISYY54 pKa = 9.79 YY55 pKa = 10.71 NFSEE59 pKa = 4.3 MQQHH63 pKa = 5.92 LMRR66 pKa = 11.84 RR67 pKa = 11.84 EE68 pKa = 4.17 RR69 pKa = 11.84 ILSEE73 pKa = 3.74 NLIEE77 pKa = 5.08 RR78 pKa = 11.84 DD79 pKa = 3.4 KK80 pKa = 11.59 LYY82 pKa = 10.86 DD83 pKa = 3.3 AEE85 pKa = 3.98 IRR87 pKa = 11.84 KK88 pKa = 10.0 RR89 pKa = 11.84 NEE91 pKa = 3.53 CYY93 pKa = 10.25 AKK95 pKa = 10.07 HH96 pKa = 6.11 KK97 pKa = 10.16 RR98 pKa = 11.84 RR99 pKa = 11.84 SRR101 pKa = 11.84 KK102 pKa = 9.46 RR103 pKa = 11.84 FSLYY107 pKa = 10.63 SLLYY111 pKa = 9.88 RR112 pKa = 4.76
Molecular weight: 13.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.794
IPC2_protein 8.902
IPC_protein 9.033
Toseland 8.96
ProMoST 9.209
Dawson 9.37
Bjellqvist 9.326
Wikipedia 9.633
Rodwell 9.399
Grimsley 9.472
Solomon 9.472
Lehninger 9.414
Nozaki 9.238
DTASelect 9.238
Thurlkill 9.209
EMBOSS 9.458
Sillero 9.399
Patrickios 4.024
IPC_peptide 9.458
IPC2_peptide 8.419
IPC2.peptide.svr19 7.992
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12
0
12
6042
51
3185
503.5
56.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.991 ± 0.714
2.234 ± 0.222
5.793 ± 0.612
4.651 ± 0.566
4.187 ± 0.443
5.627 ± 0.606
1.953 ± 0.273
6.256 ± 0.786
5.826 ± 0.411
9.335 ± 0.775
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.383 ± 0.264
4.982 ± 0.447
3.956 ± 0.564
2.052 ± 0.122
6.521 ± 0.736
8.739 ± 0.524
5.412 ± 0.423
8.342 ± 0.795
0.927 ± 0.11
4.833 ± 0.656
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here