Moraxella phage Mcat28
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PJV6|A0A0R6PJV6_9CAUD Uncharacterized protein OS=Moraxella phage Mcat28 OX=1647544 PE=4 SV=1
MM1 pKa = 7.73 LNRR4 pKa = 11.84 KK5 pKa = 8.29 SVEE8 pKa = 3.42 LFYY11 pKa = 10.69 WVNHH15 pKa = 5.39 EE16 pKa = 4.32 MNASRR21 pKa = 11.84 IIYY24 pKa = 8.12 EE25 pKa = 4.05 KK26 pKa = 10.54 LAPLVDD32 pKa = 3.52 SRR34 pKa = 11.84 VYY36 pKa = 10.48 PLFVPEE42 pKa = 4.73 SVGQRR47 pKa = 11.84 PPYY50 pKa = 10.0 IVYY53 pKa = 8.12 QTISTQPDD61 pKa = 3.13 NTLDD65 pKa = 4.44 GITGHH70 pKa = 5.86 EE71 pKa = 4.5 WINIQIDD78 pKa = 4.47 VYY80 pKa = 11.32 HH81 pKa = 6.95 NDD83 pKa = 3.56 YY84 pKa = 11.67 DD85 pKa = 5.25 DD86 pKa = 4.45 MLALTDD92 pKa = 3.72 QVISVLDD99 pKa = 4.0 NIKK102 pKa = 9.54 PSEE105 pKa = 4.16 YY106 pKa = 10.69 GGCQYY111 pKa = 10.98 LYY113 pKa = 10.79 EE114 pKa = 5.38 DD115 pKa = 3.74 GLYY118 pKa = 10.44 RR119 pKa = 11.84 SLIEE123 pKa = 3.73 YY124 pKa = 8.58 GFWQTYY130 pKa = 7.57
Molecular weight: 15.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.271
IPC2_protein 4.406
IPC_protein 4.317
Toseland 4.139
ProMoST 4.457
Dawson 4.291
Bjellqvist 4.431
Wikipedia 4.19
Rodwell 4.151
Grimsley 4.05
Solomon 4.279
Lehninger 4.24
Nozaki 4.406
DTASelect 4.596
Thurlkill 4.164
EMBOSS 4.202
Sillero 4.431
Patrickios 0.375
IPC_peptide 4.279
IPC2_peptide 4.418
IPC2.peptide.svr19 4.347
Protein with the highest isoelectric point:
>tr|A0A0R6PK39|A0A0R6PK39_9CAUD Tail protein OS=Moraxella phage Mcat28 OX=1647544 PE=4 SV=1
MM1 pKa = 7.15 KK2 pKa = 10.48 VSVKK6 pKa = 10.34 IEE8 pKa = 3.9 GLKK11 pKa = 10.69 EE12 pKa = 3.69 LDD14 pKa = 3.64 EE15 pKa = 4.12 QLGKK19 pKa = 9.65 IDD21 pKa = 3.55 KK22 pKa = 9.69 EE23 pKa = 4.12 FRR25 pKa = 11.84 GKK27 pKa = 10.07 SLYY30 pKa = 10.37 EE31 pKa = 3.95 SLNFASQPMLNAARR45 pKa = 11.84 RR46 pKa = 11.84 GARR49 pKa = 11.84 VAEE52 pKa = 3.93 KK53 pKa = 10.16 RR54 pKa = 11.84 YY55 pKa = 9.61 RR56 pKa = 11.84 RR57 pKa = 11.84 YY58 pKa = 9.05 MSHH61 pKa = 6.08 GQGEE65 pKa = 4.36 ATYY68 pKa = 11.15 VKK70 pKa = 9.44 TKK72 pKa = 9.15 NGKK75 pKa = 7.56 WRR77 pKa = 11.84 AGKK80 pKa = 9.56 SKK82 pKa = 10.08 RR83 pKa = 11.84 AKK85 pKa = 9.61 RR86 pKa = 11.84 GEE88 pKa = 4.3 GKK90 pKa = 10.53 FEE92 pKa = 3.85 WQEE95 pKa = 3.59 PGLLKK100 pKa = 10.63 KK101 pKa = 10.01 SVRR104 pKa = 11.84 RR105 pKa = 11.84 RR106 pKa = 11.84 RR107 pKa = 11.84 LNKK110 pKa = 9.46 KK111 pKa = 9.66 SKK113 pKa = 8.28 NKK115 pKa = 9.38 HH116 pKa = 5.0 RR117 pKa = 11.84 ASVGIYY123 pKa = 6.38 MHH125 pKa = 7.01 KK126 pKa = 10.08 GTGKK130 pKa = 7.35 THH132 pKa = 6.21 GSAYY136 pKa = 7.75 YY137 pKa = 9.61 WRR139 pKa = 11.84 FIEE142 pKa = 4.36 YY143 pKa = 7.92 GTKK146 pKa = 10.32 KK147 pKa = 9.82 MPATPFIRR155 pKa = 11.84 PAYY158 pKa = 8.92 HH159 pKa = 5.27 NHH161 pKa = 5.33 EE162 pKa = 4.56 AEE164 pKa = 3.98 AVDD167 pKa = 3.77 RR168 pKa = 11.84 FKK170 pKa = 11.5 SRR172 pKa = 11.84 LAKK175 pKa = 10.25 SIKK178 pKa = 9.81 KK179 pKa = 9.99 HH180 pKa = 5.5 GGG182 pKa = 3.1
Molecular weight: 21.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.271
IPC2_protein 9.94
IPC_protein 10.423
Toseland 10.862
ProMoST 10.423
Dawson 10.95
Bjellqvist 10.584
Wikipedia 11.096
Rodwell 11.418
Grimsley 10.979
Solomon 11.008
Lehninger 10.994
Nozaki 10.818
DTASelect 10.584
Thurlkill 10.847
EMBOSS 11.242
Sillero 10.862
Patrickios 11.125
IPC_peptide 11.023
IPC2_peptide 9.165
IPC2.peptide.svr19 8.57
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
86
0
86
16520
37
1175
192.1
21.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.245 ± 0.443
1.108 ± 0.149
6.277 ± 0.204
5.823 ± 0.195
3.674 ± 0.164
6.386 ± 0.254
2.421 ± 0.188
6.495 ± 0.195
7.222 ± 0.262
8.916 ± 0.248
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.694 ± 0.147
5.291 ± 0.22
3.281 ± 0.201
4.534 ± 0.186
4.594 ± 0.247
6.459 ± 0.352
6.096 ± 0.317
5.751 ± 0.203
1.241 ± 0.092
3.493 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here