Leptonychotes weddellii (Weddell seal) (Otaria weddellii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Carnivora;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 34224 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U3YBR7|A0A2U3YBR7_LEPWE PAK4-inhibitor INKA2 OS=Leptonychotes weddellii OX=9713 GN=INKA2 PE=3 SV=1
LL1 pKa = 8.17VCILCSQDD9 pKa = 3.35NCPLLPNSGQEE20 pKa = 4.8DD21 pKa = 4.05FDD23 pKa = 5.49KK24 pKa = 11.33DD25 pKa = 3.82GTGDD29 pKa = 3.68ACDD32 pKa = 4.45EE33 pKa = 4.97DD34 pKa = 5.18DD35 pKa = 6.3DD36 pKa = 5.46NDD38 pKa = 4.45GVDD41 pKa = 4.91DD42 pKa = 5.27EE43 pKa = 5.57KK44 pKa = 11.77DD45 pKa = 3.31NCQLLFNPRR54 pKa = 11.84QFDD57 pKa = 3.57YY58 pKa = 11.45DD59 pKa = 3.52KK60 pKa = 11.69DD61 pKa = 4.14EE62 pKa = 4.7VGDD65 pKa = 3.79RR66 pKa = 11.84CDD68 pKa = 3.2NCPYY72 pKa = 10.11VHH74 pKa = 6.82NPAQIDD80 pKa = 3.5TDD82 pKa = 3.98NNGEE86 pKa = 3.91GDD88 pKa = 3.81ACSVDD93 pKa = 3.02IDD95 pKa = 4.08GDD97 pKa = 4.08DD98 pKa = 3.82VFNEE102 pKa = 4.26RR103 pKa = 11.84DD104 pKa = 3.43NCPYY108 pKa = 10.72VYY110 pKa = 9.89NTDD113 pKa = 3.55QRR115 pKa = 11.84DD116 pKa = 3.41TDD118 pKa = 3.67GDD120 pKa = 4.33GVGDD124 pKa = 3.92HH125 pKa = 7.43CDD127 pKa = 3.4NCPLVHH133 pKa = 6.91NPDD136 pKa = 3.37QTDD139 pKa = 2.77VDD141 pKa = 3.78NDD143 pKa = 4.03LVGDD147 pKa = 3.72QCDD150 pKa = 3.64NNEE153 pKa = 5.1DD154 pKa = 3.49IDD156 pKa = 4.85EE157 pKa = 5.38DD158 pKa = 3.95GHH160 pKa = 6.99QNNQDD165 pKa = 3.17NCPYY169 pKa = 10.33ISNANQADD177 pKa = 3.98HH178 pKa = 7.28DD179 pKa = 4.49HH180 pKa = 7.58DD181 pKa = 4.53GQGDD185 pKa = 3.81ACDD188 pKa = 4.6SDD190 pKa = 4.48DD191 pKa = 6.02DD192 pKa = 4.76NDD194 pKa = 4.26GVPDD198 pKa = 4.51DD199 pKa = 4.98RR200 pKa = 11.84DD201 pKa = 3.34NCRR204 pKa = 11.84LVSNPGQEE212 pKa = 5.02DD213 pKa = 3.49SDD215 pKa = 4.2GDD217 pKa = 4.03GRR219 pKa = 11.84GDD221 pKa = 3.29ACKK224 pKa = 10.67DD225 pKa = 3.34DD226 pKa = 4.2FDD228 pKa = 4.98NDD230 pKa = 4.83RR231 pKa = 11.84IPDD234 pKa = 3.63IDD236 pKa = 4.49DD237 pKa = 3.64VCPEE241 pKa = 3.75NSAISEE247 pKa = 4.02TDD249 pKa = 3.4FRR251 pKa = 11.84NFQMVHH257 pKa = 7.1LDD259 pKa = 3.63PKK261 pKa = 10.23GTTQIDD267 pKa = 4.0PNWVIRR273 pKa = 11.84HH274 pKa = 4.99QGKK277 pKa = 9.11EE278 pKa = 3.87LVQTANSDD286 pKa = 3.28PGIAVGFDD294 pKa = 3.2EE295 pKa = 5.37FGSVDD300 pKa = 3.7FSGTFYY306 pKa = 11.53VNTDD310 pKa = 3.3RR311 pKa = 11.84DD312 pKa = 3.36DD313 pKa = 4.91DD314 pKa = 4.04YY315 pKa = 12.01AGFVFGYY322 pKa = 9.43QSSSRR327 pKa = 11.84FYY329 pKa = 10.25VVMWKK334 pKa = 9.82QVTQTYY340 pKa = 8.78WEE342 pKa = 4.39DD343 pKa = 3.58QPTRR347 pKa = 11.84AYY349 pKa = 10.06GYY351 pKa = 9.91SGVSLKK357 pKa = 10.51VVNSTTGTGEE367 pKa = 4.03HH368 pKa = 6.41LRR370 pKa = 11.84NALWHH375 pKa = 5.99TGNTEE380 pKa = 4.02GQVRR384 pKa = 11.84TLWHH388 pKa = 6.69DD389 pKa = 3.57PKK391 pKa = 11.28NIGWKK396 pKa = 10.31DD397 pKa = 3.26YY398 pKa = 9.58TAYY401 pKa = 10.32RR402 pKa = 11.84WHH404 pKa = 6.5LTHH407 pKa = 7.17RR408 pKa = 11.84PKK410 pKa = 10.37TGYY413 pKa = 9.82IRR415 pKa = 11.84VLVHH419 pKa = 6.46EE420 pKa = 5.84GKK422 pKa = 9.99QVMADD427 pKa = 3.41SGPVYY432 pKa = 10.46DD433 pKa = 3.47QTYY436 pKa = 9.92AGGRR440 pKa = 11.84LGLFVFSQEE449 pKa = 3.65MVYY452 pKa = 10.62FSDD455 pKa = 4.84LKK457 pKa = 10.82YY458 pKa = 10.49EE459 pKa = 4.28CRR461 pKa = 11.84DD462 pKa = 3.3VV463 pKa = 3.44

Molecular weight: 51.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7F8QEU1|A0A7F8QEU1_LEPWE sodium/hydrogen exchanger 8 OS=Leptonychotes weddellii OX=9713 GN=LOC115938833 PE=3 SV=1
RR1 pKa = 7.42PRR3 pKa = 11.84NAQSLPQKK11 pKa = 9.78PRR13 pKa = 11.84NAQSLPQRR21 pKa = 11.84PQNAQNLPQRR31 pKa = 11.84PQNIVRR37 pKa = 11.84LPRR40 pKa = 11.84RR41 pKa = 11.84PRR43 pKa = 11.84NAQSLPRR50 pKa = 11.84RR51 pKa = 11.84AQNILSLPQRR61 pKa = 11.84PRR63 pKa = 11.84NAQSLPQRR71 pKa = 11.84PQNTQNLPQRR81 pKa = 11.84PRR83 pKa = 11.84NIVNLPRR90 pKa = 11.84RR91 pKa = 11.84PRR93 pKa = 11.84NIVSLPGRR101 pKa = 11.84LRR103 pKa = 11.84NAQSLPWRR111 pKa = 11.84PRR113 pKa = 11.84NILNLPQRR121 pKa = 11.84PRR123 pKa = 11.84NAQSLPRR130 pKa = 11.84KK131 pKa = 8.74PRR133 pKa = 11.84NAQSLSRR140 pKa = 11.84RR141 pKa = 11.84PLNTQSLPRR150 pKa = 11.84GPRR153 pKa = 11.84NAQSLPQRR161 pKa = 11.84PWNAQSLPCGSQNAQSLSQRR181 pKa = 11.84PWNAQSLPQGSRR193 pKa = 11.84SILSLPRR200 pKa = 11.84KK201 pKa = 9.26LRR203 pKa = 11.84NAQSLPRR210 pKa = 11.84RR211 pKa = 11.84PQNTQSLQQKK221 pKa = 7.64PQNILSLSQRR231 pKa = 11.84PQNAQNLPRR240 pKa = 11.84RR241 pKa = 11.84PQNILSLPQKK251 pKa = 9.74PRR253 pKa = 11.84NAQSLPRR260 pKa = 11.84RR261 pKa = 11.84PQNTQSLPRR270 pKa = 11.84RR271 pKa = 11.84PQNAQNLPQRR281 pKa = 11.84PQNAQSLPQRR291 pKa = 11.84PQNTQNLQRR300 pKa = 11.84RR301 pKa = 11.84PQNAQNLPQGPQNILSLPQRR321 pKa = 11.84PQNAQSLPP329 pKa = 3.6

Molecular weight: 37.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22127

12097

34224

18266267

31

34619

533.7

59.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.002 ± 0.015

2.159 ± 0.011

4.79 ± 0.008

7.202 ± 0.019

3.562 ± 0.009

6.589 ± 0.018

2.617 ± 0.007

4.203 ± 0.012

5.763 ± 0.019

9.918 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.006

3.509 ± 0.011

6.465 ± 0.024

4.856 ± 0.013

5.796 ± 0.015

8.469 ± 0.019

5.216 ± 0.01

5.965 ± 0.012

1.188 ± 0.005

2.563 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski