Salinibacter sp. 10B

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidetes Order II. Incertae sedis; Rhodothermaceae; Salinibacter; unclassified Salinibacter

Average proteome isoelectric point is 5.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3709 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P6BWT0|A0A2P6BWT0_9BACT Robl_LC7 domain-containing protein OS=Salinibacter sp. 10B OX=1923971 GN=BSZ35_00815 PE=4 SV=1
MM1 pKa = 7.84RR2 pKa = 11.84SHH4 pKa = 6.37NRR6 pKa = 11.84LRR8 pKa = 11.84QAFNRR13 pKa = 11.84LRR15 pKa = 11.84ILAAGILVLCVCATGVGCGGAGSNDD40 pKa = 3.34DD41 pKa = 4.43PGPSEE46 pKa = 5.06EE47 pKa = 4.55EE48 pKa = 4.11APPPTPTDD56 pKa = 3.43LEE58 pKa = 4.69GNSEE62 pKa = 4.01NSLVEE67 pKa = 4.02LTWNGVNDD75 pKa = 4.85DD76 pKa = 4.88DD77 pKa = 5.17LGGYY81 pKa = 8.19NVYY84 pKa = 10.59RR85 pKa = 11.84DD86 pKa = 3.79TDD88 pKa = 4.67PIDD91 pKa = 3.99GVSGLSPEE99 pKa = 4.2NGSPISSTSYY109 pKa = 10.88SDD111 pKa = 4.95EE112 pKa = 3.92EE113 pKa = 4.57VQNGTPYY120 pKa = 10.35YY121 pKa = 9.81YY122 pKa = 10.94VVTAEE127 pKa = 5.12DD128 pKa = 3.88EE129 pKa = 4.53DD130 pKa = 5.13SNEE133 pKa = 4.03SDD135 pKa = 3.57PSNEE139 pKa = 3.96VEE141 pKa = 4.55KK142 pKa = 10.43TPFDD146 pKa = 4.4DD147 pKa = 4.83PPNRR151 pKa = 11.84PP152 pKa = 3.22

Molecular weight: 16.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P6BWB4|A0A2P6BWB4_9BACT His_kinase domain-containing protein OS=Salinibacter sp. 10B OX=1923971 GN=BSZ35_18555 PE=4 SV=1
MM1 pKa = 7.24RR2 pKa = 11.84TSPSCILTADD12 pKa = 3.88RR13 pKa = 11.84QPIRR17 pKa = 11.84RR18 pKa = 11.84GRR20 pKa = 11.84LALVTVFQYY29 pKa = 10.14MEE31 pKa = 4.24NLSVRR36 pKa = 11.84QKK38 pKa = 11.0AVRR41 pKa = 11.84ARR43 pKa = 11.84LDD45 pKa = 2.98WKK47 pKa = 10.71YY48 pKa = 11.55ALGLALIDD56 pKa = 3.55SGFEE60 pKa = 3.76RR61 pKa = 11.84TVLCEE66 pKa = 3.39FRR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84LVRR73 pKa = 11.84AGAGPFCWIDD83 pKa = 3.12WKK85 pKa = 11.23SEE87 pKa = 3.75EE88 pKa = 4.33SKK90 pKa = 10.32EE91 pKa = 3.8WRR93 pKa = 11.84QGRR96 pKa = 11.84FIGKK100 pKa = 9.46VGICKK105 pKa = 9.6EE106 pKa = 4.0CSSRR110 pKa = 11.84RR111 pKa = 11.84FVSGLHH117 pKa = 6.53DD118 pKa = 3.71AAFYY122 pKa = 10.94VATLRR127 pKa = 11.84QGDD130 pKa = 3.7SRR132 pKa = 11.84EE133 pKa = 4.0TLRR136 pKa = 11.84PQGLAEE142 pKa = 4.01RR143 pKa = 11.84AQRR146 pKa = 11.84KK147 pKa = 7.75PRR149 pKa = 11.84SS150 pKa = 3.51

Molecular weight: 17.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3709

0

3709

1286740

26

3361

346.9

38.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.572 ± 0.053

0.62 ± 0.01

6.759 ± 0.036

7.315 ± 0.048

3.53 ± 0.025

7.976 ± 0.044

2.249 ± 0.021

4.166 ± 0.03

2.469 ± 0.027

9.759 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.982 ± 0.02

2.831 ± 0.025

5.226 ± 0.033

3.838 ± 0.027

7.107 ± 0.043

6.269 ± 0.038

6.395 ± 0.036

7.695 ± 0.035

1.365 ± 0.018

2.879 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski