Calidithermus roseus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Calidithermus

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3553 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A399EVX4|A0A399EVX4_9DEIN Uncharacterized protein OS=Calidithermus roseus OX=1644118 GN=Mrose_01180 PE=4 SV=1
MM1 pKa = 7.9DD2 pKa = 4.44EE3 pKa = 5.24KK4 pKa = 10.49IIRR7 pKa = 11.84YY8 pKa = 9.14NPDD11 pKa = 2.83GSVMAEE17 pKa = 4.07VEE19 pKa = 3.89WRR21 pKa = 11.84GPGDD25 pKa = 3.47YY26 pKa = 10.22TVVNGALDD34 pKa = 3.92EE35 pKa = 4.4EE36 pKa = 5.19GKK38 pKa = 10.81AILDD42 pKa = 3.79AVGDD46 pKa = 3.48IYY48 pKa = 11.13GYY50 pKa = 10.66VEE52 pKa = 5.27GYY54 pKa = 9.65TNVNDD59 pKa = 4.1AYY61 pKa = 10.39EE62 pKa = 4.2DD63 pKa = 3.31RR64 pKa = 11.84PYY66 pKa = 10.88FGWPEE71 pKa = 3.8EE72 pKa = 4.27KK73 pKa = 10.72

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A399EWH1|A0A399EWH1_9DEIN 30S ribosomal protein S3 OS=Calidithermus roseus OX=1644118 GN=rpsC PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 9.47RR3 pKa = 11.84TWQPNKK9 pKa = 9.68RR10 pKa = 11.84KK11 pKa = 9.52RR12 pKa = 11.84AKK14 pKa = 8.26THH16 pKa = 5.09GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.07TKK25 pKa = 10.29NGRR28 pKa = 11.84KK29 pKa = 8.9VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84ARR41 pKa = 11.84LSVSGG46 pKa = 3.91

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3553

0

3553

1074463

30

2742

302.4

33.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.895 ± 0.05

0.603 ± 0.011

4.111 ± 0.028

7.062 ± 0.047

3.602 ± 0.027

8.83 ± 0.038

1.972 ± 0.021

3.951 ± 0.031

3.175 ± 0.038

12.643 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.837 ± 0.015

2.324 ± 0.029

5.793 ± 0.03

3.803 ± 0.03

7.581 ± 0.04

5.131 ± 0.034

4.497 ± 0.031

7.68 ± 0.037

1.622 ± 0.019

2.887 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski