Panacagrimonas perspica
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4772 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R7NXK3|A0A4R7NXK3_9GAMM Uncharacterized protein OS=Panacagrimonas perspica OX=381431 GN=DFR24_4352 PE=4 SV=1
MM1 pKa = 7.13 KK2 pKa = 10.18 RR3 pKa = 11.84 EE4 pKa = 4.51 LIAAIAACGFAAPALAATGNNYY26 pKa = 9.7 VDD28 pKa = 3.89 GYY30 pKa = 10.99 FYY32 pKa = 11.09 LSDD35 pKa = 3.76 IDD37 pKa = 4.86 VGPADD42 pKa = 4.57 DD43 pKa = 5.49 DD44 pKa = 4.45 GVGVGVKK51 pKa = 10.38 GGFQVADD58 pKa = 3.38 QVYY61 pKa = 7.95 LTGEE65 pKa = 4.28 YY66 pKa = 7.98 QTVEE70 pKa = 3.86 YY71 pKa = 11.02 DD72 pKa = 4.08 DD73 pKa = 5.55 FNVDD77 pKa = 4.0 LDD79 pKa = 3.8 QLRR82 pKa = 11.84 LGAGFGPGMGTKK94 pKa = 10.36 GQGLYY99 pKa = 10.82 GRR101 pKa = 11.84 AEE103 pKa = 4.06 YY104 pKa = 11.12 VKK106 pKa = 10.68 FDD108 pKa = 4.2 IDD110 pKa = 4.21 SEE112 pKa = 4.34 DD113 pKa = 3.49 DD114 pKa = 3.54 SGIGGHH120 pKa = 6.08 VGYY123 pKa = 10.61 ALPLSDD129 pKa = 3.44 QFRR132 pKa = 11.84 LHH134 pKa = 6.99 GEE136 pKa = 3.91 VGYY139 pKa = 11.02 LLLDD143 pKa = 4.46 DD144 pKa = 4.83 SDD146 pKa = 4.61 GPEE149 pKa = 3.84 LAIGGTYY156 pKa = 9.73 RR157 pKa = 11.84 VAQNFGIFADD167 pKa = 4.09 YY168 pKa = 10.22 RR169 pKa = 11.84 ASFLDD174 pKa = 3.97 GDD176 pKa = 4.18 HH177 pKa = 6.99 GGDD180 pKa = 4.69 LDD182 pKa = 4.96 ISDD185 pKa = 4.56 LRR187 pKa = 11.84 IGARR191 pKa = 11.84 FYY193 pKa = 11.22 FF194 pKa = 4.26
Molecular weight: 20.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.91
IPC_protein 3.923
Toseland 3.694
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.897
Rodwell 3.745
Grimsley 3.605
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.342
Thurlkill 3.757
EMBOSS 3.91
Sillero 4.05
Patrickios 1.189
IPC_peptide 3.923
IPC2_peptide 4.024
IPC2.peptide.svr19 3.921
Protein with the highest isoelectric point:
>tr|A0A4R7P0E0|A0A4R7P0E0_9GAMM Uncharacterized protein OS=Panacagrimonas perspica OX=381431 GN=DFR24_3742 PE=4 SV=1
MM1 pKa = 7.43 LVLFALGICIGLTLGLTGAGGSIIAVPILMFGMGWTVAQAGPVALLAVASSAAVGAMIGLRR62 pKa = 11.84 QRR64 pKa = 11.84 QVRR67 pKa = 11.84 YY68 pKa = 9.26 RR69 pKa = 11.84 AAILVASCGVVVSPAGLMTAKK90 pKa = 10.31 HH91 pKa = 5.93 IPPHH95 pKa = 6.22 LLTVAFAALLAWASFTALKK114 pKa = 10.23 KK115 pKa = 10.61 SRR117 pKa = 11.84 GSSHH121 pKa = 6.67 VGVSEE126 pKa = 4.36 SVGGDD131 pKa = 3.25 GASCLRR137 pKa = 11.84 DD138 pKa = 3.0 AATGRR143 pKa = 11.84 FCWTRR148 pKa = 11.84 SCGQALAITGVGAGFLSGLFGVGGGFFIVPALRR181 pKa = 11.84 RR182 pKa = 11.84 ITDD185 pKa = 3.76 LSMNAVIATSMLIIALVSSGAAISAALAGRR215 pKa = 11.84 LPLDD219 pKa = 3.33 VGLPFAFGAVCGMAGGRR236 pKa = 11.84 MVSGKK241 pKa = 10.25 VDD243 pKa = 3.49 GVLLARR249 pKa = 11.84 GFSALCGVIAVLLVARR265 pKa = 11.84 EE266 pKa = 4.03 IYY268 pKa = 10.07 AWRR271 pKa = 4.39
Molecular weight: 27.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.385
IPC_protein 10.043
Toseland 10.409
ProMoST 10.438
Dawson 10.54
Bjellqvist 10.306
Wikipedia 10.73
Rodwell 10.672
Grimsley 10.584
Solomon 10.657
Lehninger 10.628
Nozaki 10.54
DTASelect 10.262
Thurlkill 10.438
EMBOSS 10.818
Sillero 10.496
Patrickios 10.452
IPC_peptide 10.657
IPC2_peptide 9.867
IPC2.peptide.svr19 8.549
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4772
0
4772
1604628
29
2717
336.3
36.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.995 ± 0.048
0.97 ± 0.012
5.654 ± 0.023
5.641 ± 0.03
3.654 ± 0.024
8.373 ± 0.032
2.19 ± 0.018
4.49 ± 0.024
3.343 ± 0.032
10.424 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.336 ± 0.017
2.565 ± 0.023
5.341 ± 0.026
3.442 ± 0.021
7.531 ± 0.039
5.622 ± 0.023
5.131 ± 0.027
7.496 ± 0.03
1.474 ± 0.016
2.326 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here