Streptococcus satellite phage Javan307
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZIV5|A0A4D5ZIV5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan307 OX=2558626 GN=JavanS307_0007 PE=4 SV=1
MM1 pKa = 7.54 IADD4 pKa = 3.78 KK5 pKa = 11.39 LEE7 pKa = 4.85 AISTNLEE14 pKa = 4.11 EE15 pKa = 4.13 IQEE18 pKa = 4.13 ILVGNGTALAKK29 pKa = 10.45 RR30 pKa = 11.84 EE31 pKa = 3.86 ISKK34 pKa = 10.38 VLNDD38 pKa = 4.47 LDD40 pKa = 5.59 DD41 pKa = 4.4 IYY43 pKa = 11.79 NEE45 pKa = 4.13 LTSTEE50 pKa = 3.99 YY51 pKa = 10.82 HH52 pKa = 5.9 EE53 pKa = 4.26 QQEE56 pKa = 4.54 TLKK59 pKa = 10.72 EE60 pKa = 3.7 QTEE63 pKa = 4.21 RR64 pKa = 11.84 MKK66 pKa = 10.83 QRR68 pKa = 11.84 VVSEE72 pKa = 4.31 VIGEE76 pKa = 4.27 LEE78 pKa = 3.68 WRR80 pKa = 11.84 EE81 pKa = 3.45 YY82 pKa = 11.05 SYY84 pKa = 11.97 YY85 pKa = 10.94 KK86 pKa = 10.66 NFWGDD91 pKa = 3.24 MDD93 pKa = 5.26 LLKK96 pKa = 10.88 CLSNFDD102 pKa = 3.56 GFLFISTYY110 pKa = 10.01 LAEE113 pKa = 4.1 ITKK116 pKa = 10.6 EE117 pKa = 3.51 NDD119 pKa = 2.93 YY120 pKa = 11.52 PMDD123 pKa = 4.03 KK124 pKa = 10.74 NQVLNYY130 pKa = 9.47 VWEE133 pKa = 4.22 LLAIDD138 pKa = 3.6 IAKK141 pKa = 10.3 KK142 pKa = 10.58 KK143 pKa = 9.91 RR144 pKa = 11.84 DD145 pKa = 3.7 KK146 pKa = 11.36 KK147 pKa = 10.98 NLLDD151 pKa = 3.74 LWTSSIEE158 pKa = 4.03 YY159 pKa = 8.46 TRR161 pKa = 11.84 GMMIYY166 pKa = 10.2 EE167 pKa = 4.29 EE168 pKa = 4.23 NN169 pKa = 3.33
Molecular weight: 20.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.583
IPC2_protein 4.571
IPC_protein 4.482
Toseland 4.329
ProMoST 4.571
Dawson 4.418
Bjellqvist 4.558
Wikipedia 4.266
Rodwell 4.317
Grimsley 4.24
Solomon 4.406
Lehninger 4.368
Nozaki 4.52
DTASelect 4.647
Thurlkill 4.317
EMBOSS 4.279
Sillero 4.584
Patrickios 3.821
IPC_peptide 4.418
IPC2_peptide 4.571
IPC2.peptide.svr19 4.554
Protein with the highest isoelectric point:
>tr|A0A4D5ZJP7|A0A4D5ZJP7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan307 OX=2558626 GN=JavanS307_0003 PE=4 SV=1
MM1 pKa = 7.84 KK2 pKa = 9.12 ITEE5 pKa = 4.39 VIKK8 pKa = 10.77 KK9 pKa = 10.02 DD10 pKa = 3.19 GSKK13 pKa = 10.33 VYY15 pKa = 10.14 RR16 pKa = 11.84 ANVYY20 pKa = 10.6 LGVDD24 pKa = 3.13 QVTGKK29 pKa = 10.2 KK30 pKa = 10.32 VKK32 pKa = 9.81 TKK34 pKa = 9.67 VTGRR38 pKa = 11.84 TQKK41 pKa = 10.07 EE42 pKa = 4.39 VKK44 pKa = 10.08 QKK46 pKa = 11.27 ANQEE50 pKa = 3.99 KK51 pKa = 10.29 IAFQKK56 pKa = 10.81 AGSTRR61 pKa = 11.84 QKK63 pKa = 11.02 AITIKK68 pKa = 10.49 NYY70 pKa = 10.3 QEE72 pKa = 5.26 LATLWWEE79 pKa = 4.3 SYY81 pKa = 11.07 KK82 pKa = 10.18 NTVKK86 pKa = 10.71 PNTQGNVRR94 pKa = 11.84 ALIDD98 pKa = 3.5 NHH100 pKa = 6.36 ILPTFGEE107 pKa = 4.29 YY108 pKa = 10.7 KK109 pKa = 10.12 LDD111 pKa = 3.72 KK112 pKa = 10.14 LTTPLIQSIINKK124 pKa = 9.52 LADD127 pKa = 3.2 KK128 pKa = 10.1 TNRR131 pKa = 11.84 GEE133 pKa = 4.1 KK134 pKa = 10.44 GAFLHH139 pKa = 6.19 YY140 pKa = 10.82 DD141 pKa = 4.02 KK142 pKa = 11.05 IHH144 pKa = 6.59 ALNKK148 pKa = 10.18 RR149 pKa = 11.84 ILQYY153 pKa = 10.89 GVTMQAIPSNPARR166 pKa = 11.84 DD167 pKa = 3.48 VVLPRR172 pKa = 11.84 NTQKK176 pKa = 11.19 AKK178 pKa = 8.99 RR179 pKa = 11.84 QKK181 pKa = 10.16 VKK183 pKa = 10.6 HH184 pKa = 5.86 FEE186 pKa = 3.95 NQEE189 pKa = 3.86 LKK191 pKa = 10.93 KK192 pKa = 10.77 FLGYY196 pKa = 10.55 LDD198 pKa = 5.12 NLDD201 pKa = 3.56 SDD203 pKa = 4.32 RR204 pKa = 11.84 YY205 pKa = 8.96 RR206 pKa = 11.84 YY207 pKa = 10.17 YY208 pKa = 11.31 YY209 pKa = 8.28 EE210 pKa = 3.62 TTLYY214 pKa = 10.88 KK215 pKa = 10.46 FLLATGCRR223 pKa = 11.84 INEE226 pKa = 4.0 ALALSWSDD234 pKa = 2.88 IDD236 pKa = 4.45 LDD238 pKa = 3.81 NAVVHH243 pKa = 5.54 ITKK246 pKa = 9.23 TLNRR250 pKa = 11.84 DD251 pKa = 3.49 LEE253 pKa = 4.53 INSPKK258 pKa = 10.62 SKK260 pKa = 10.68 ASYY263 pKa = 10.37 RR264 pKa = 11.84 DD265 pKa = 2.95 IDD267 pKa = 3.41 IDD269 pKa = 3.65 QATVSMLKK277 pKa = 9.91 QYY279 pKa = 11.27 KK280 pKa = 9.69 LRR282 pKa = 11.84 QTKK285 pKa = 7.97 EE286 pKa = 3.19 AWKK289 pKa = 9.48 IGQRR293 pKa = 11.84 EE294 pKa = 4.21 SVVFSDD300 pKa = 6.46 FIHH303 pKa = 7.06 EE304 pKa = 4.42 YY305 pKa = 9.91 PSSSRR310 pKa = 11.84 LKK312 pKa = 10.58 RR313 pKa = 11.84 RR314 pKa = 11.84 LQTHH318 pKa = 6.76 FKK320 pKa = 10.46 RR321 pKa = 11.84 ADD323 pKa = 3.23 VPNIGFHH330 pKa = 6.1 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.03 KK348 pKa = 10.3 EE349 pKa = 3.58 LQYY352 pKa = 11.41 RR353 pKa = 11.84 LGHH356 pKa = 5.67 STLSMTMDD364 pKa = 3.58 IYY366 pKa = 11.68 SHH368 pKa = 6.98 LSKK371 pKa = 11.0 EE372 pKa = 4.25 NAKK375 pKa = 10.37 KK376 pKa = 10.37 AVSFYY381 pKa = 9.42 EE382 pKa = 4.24 TALKK386 pKa = 10.67 ALL388 pKa = 4.15
Molecular weight: 44.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.066
IPC2_protein 9.326
IPC_protein 9.224
Toseland 10.028
ProMoST 9.663
Dawson 10.218
Bjellqvist 9.853
Wikipedia 10.365
Rodwell 10.76
Grimsley 10.277
Solomon 10.233
Lehninger 10.189
Nozaki 9.999
DTASelect 9.853
Thurlkill 10.072
EMBOSS 10.423
Sillero 10.116
Patrickios 10.321
IPC_peptide 10.233
IPC2_peptide 8.302
IPC2.peptide.svr19 8.295
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
2684
48
507
191.7
22.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.179 ± 0.381
0.261 ± 0.066
6.11 ± 0.498
8.793 ± 0.822
4.173 ± 0.381
4.881 ± 0.356
1.528 ± 0.279
7.004 ± 0.451
8.979 ± 0.502
10.656 ± 0.817
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.496 ± 0.339
5.365 ± 0.569
2.571 ± 0.583
4.322 ± 0.389
5.44 ± 0.357
5.775 ± 0.281
6.259 ± 0.468
4.471 ± 0.392
1.043 ± 0.156
4.694 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here