Idiomarinaceae phage Phi1M2-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088FB74|A0A088FB74_9CAUD Uncharacterized protein OS=Idiomarinaceae phage Phi1M2-2 OX=1527515 GN=M22_052 PE=4 SV=1
MM1 pKa = 7.51SNEE4 pKa = 4.21KK5 pKa = 9.0FTSGDD10 pKa = 2.88WMVSRR15 pKa = 11.84HH16 pKa = 4.95SDD18 pKa = 3.32EE19 pKa = 4.41VSVYY23 pKa = 10.38MGDD26 pKa = 3.5AVNNPYY32 pKa = 10.44EE33 pKa = 4.33YY34 pKa = 10.54NCSDD38 pKa = 2.94VWSCDD43 pKa = 3.83SYY45 pKa = 10.98WSEE48 pKa = 4.08EE49 pKa = 4.25DD50 pKa = 2.87EE51 pKa = 4.17TAIANAHH58 pKa = 7.04LISAAPDD65 pKa = 3.12MYY67 pKa = 10.65RR68 pKa = 11.84ALRR71 pKa = 11.84GIDD74 pKa = 3.37SKK76 pKa = 11.28RR77 pKa = 11.84IAVNGDD83 pKa = 3.21GTFTITLTADD93 pKa = 3.32EE94 pKa = 4.71VDD96 pKa = 4.83AIADD100 pKa = 3.87SVSKK104 pKa = 11.4AEE106 pKa = 4.44GANHH110 pKa = 7.14DD111 pKa = 3.8

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088F782|A0A088F782_9CAUD Putative capsid coat protein OS=Idiomarinaceae phage Phi1M2-2 OX=1527515 GN=M22_007 PE=4 SV=1
MM1 pKa = 7.73TDD3 pKa = 3.99LNAKK7 pKa = 7.59AQRR10 pKa = 11.84YY11 pKa = 5.14RR12 pKa = 11.84TIRR15 pKa = 11.84EE16 pKa = 3.74AAKK19 pKa = 10.15KK20 pKa = 9.54VAAEE24 pKa = 3.99RR25 pKa = 11.84AAQFTKK31 pKa = 10.27RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84DD35 pKa = 3.18AEE37 pKa = 3.81QARR40 pKa = 11.84DD41 pKa = 3.36IKK43 pKa = 10.7EE44 pKa = 4.05RR45 pKa = 11.84EE46 pKa = 4.01VV47 pKa = 3.15

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

11618

32

792

178.7

19.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.494 ± 0.348

1.231 ± 0.18

6.567 ± 0.227

7.626 ± 0.353

3.701 ± 0.176

7.204 ± 0.268

1.687 ± 0.179

5.431 ± 0.191

5.139 ± 0.474

7.213 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.143

4.235 ± 0.167

3.959 ± 0.241

4.51 ± 0.283

6.189 ± 0.295

6.24 ± 0.318

5.973 ± 0.279

6.817 ± 0.322

1.334 ± 0.095

3.064 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski